4.5 Article

Microbial decontamination of chicken using atmospheric plasma bubbles

Journal

PLASMA PROCESSES AND POLYMERS
Volume 18, Issue 1, Pages -

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/ppap.202000052

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The study demonstrates that atmospheric air plasma bubbles are an effective, energy-efficient, and residue-free alternative for decontamination, with higher plasma discharge frequencies leading to greater reduction in bacterial colony-forming units. Scanning electron microscopy images of treated bacteria show cell damage, while an increase in conductivity and various chemical concentrations in plasma-activated water contribute to the observed antimicrobial effect.
In this study, we demonstrate that atmospheric air plasma bubbles are an effective, energy-efficient, residue-free alternative to current decontamination techniques. Five to fifteen minutes of plasma-bubble treatments of inoculated chicken skin led to a significant reduction in colony-forming units (CFUs). We show that the activation efficiency is dependent on the plasma discharge frequency, with a higher one (2,000 Hz) leading to a higher CFU reduction (1.4 log) as compared with a lower (0.3 log) reduction at 1,000 Hz. Scanning electron microscopy pictures of treated bacteria reveal damage to the cells. An evaluation of the physicochemical properties of the generated plasma-activated water revealed an increase in conductivity and in ozone, nitrite, nitrate, hydroxyl, and peroxide concentrations with higher frequencies, all contributing to the observed antimicrobial effect.

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