4.5 Article

Genetic analysis, realized heritability and synergistic suppression of indoxacarb resistance in Phenacoccus solenopsis Tinsley (Homoptera: Pseudococcidae)

Journal

CROP PROTECTION
Volume 84, Issue -, Pages 62-68

Publisher

ELSEVIER SCI LTD
DOI: 10.1016/j.cropro.2016.02.009

Keywords

Indoxacarb; Resistance; Cotton; Inheritance; Synergist

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Cotton mealybug Phenacoccus solenopsis Tinsley (Homoptera: Pseudococcidae) has caused huge crop and economic losses in different countries of the world including Pakistan. Considering P. solenopsis as a key cotton pest, research was conducted to understand genetics, realized heritability and preliminary mechanism of indoxacarb resistance. As a result of selection with indoxacarb, P. solenopsis developed a 2223.13-fold resistance after five generations. Indoxacarb resistance was autosomal in nature, and the degree of dominance for F-1 and F-1 ' was 0.60 and 0.62, respectively, indicating the incomplete dominance of indoxacarb resistance. A monogenic model of inheritance also showed the involvement of multiple loci in indoxacarb resistance in P. solenopsis. Realized heritability for indoxacarb resistance was markedly high (h(2) = 1.13) in P. solenopsis suggesting how quickly resistance can develop as a result of selection. Synergism tests with piperonylbutoxide (PBO) and S,S,S-tributylphosphorotrithioate (DEF) also suggested that indoxacarb resistance in P. solenopsis was metabolism-based due to possible involvement of both mono-oxygenases and esterases. Hence, it was concluded that indoxacarb resistance in P. solenopsis was autosomal, incompletely dominant, polygenic and metabolism-based. These findings will be helpful in the management of P. solenopsis. (C) 2016 Elsevier Ltd. All rights reserved.

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