Review
Biotechnology & Applied Microbiology
Giuseppe Cala, Beatrice Sina, Paolo De Coppi, Giovanni Giuseppe Giobbe, Mattia Francesco Maria Gerli
Summary: Over the past 10 years, significant progress has been made in the field of organoids, particularly in studying organ development, homeostasis, and disease modeling in vitro. Organoid technology has been widely used to generate patient-specific 3D cultures from stem/progenitor cells, leading to the development of innovative disease models and regenerative therapies. Different organs and tissues have been successfully modeled using primary organoid culture systems, and there is ongoing research to improve their applications in various fields.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Xiaoyong Guan, Shigao Huang
Summary: Traditional tumor models are unable to accurately simulate the real state of tumors in vivo, leading to the termination of many clinical trials. However, 3D tumor models' technology offers new in vitro models that bridge the gap between in vitro and in vivo findings. Organoids, in particular, can maintain the properties of the original tissue over a long period of culture, enabling extensive research and serving as potential substitutes for animal and in vitro models. 3D tumor models also provide a promising platform for high-throughput research, drug testing, disease modeling, and regenerative medicine.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Juliana Sitta, Pier Paolo Claudio, Candace M. Howard
Summary: Immunotherapy has shown promising results in certain types of cancer, but the immune suppression mechanisms of tumors limit its efficacy. Oncolytic viruses, as a novel therapeutic approach, can enhance immune response and improve treatment outcomes through genetic engineering. However, developing preclinical models that replicate human immune response remains a challenge.
Article
Cell Biology
Bin Xue, Julia Schueler, Christopher M. Harrod, Kanstantsin Lashuk, Zoji Bomya, Kolin C. Hribar
Summary: In this study, a novel 3D in vitro tumor panel comprising 30 distinct PDX models cocultured with fibroblasts and PBMCs in planar extracellular matrix hydrogels was described. The panel represents a significant advancement toward screening in vitro models of the tumor microenvironment and can be used for rapidly screening various combinations and novel agents, accelerating drug discovery for the next generation of therapeutics.
Article
Biochemistry & Molecular Biology
Serena Calamaio, Marialaura Serzanti, Jennifer Boniotti, Annamaria Fra, Emirena Garrafa, Manuela Cominelli, Rosanna Verardi, Pietro Luigi Poliani, Silvia Dotti, Riccardo Villa, Giovanna Mazzoleni, Patrizia Dell'Era, Nathalie Steimberg
Summary: The process of developing a new drug is time-consuming and expensive, and hepatotoxicity is a major concern. Animal models have limitations due to interspecies differences, but human induced pluripotent stem cells (hiPSCs) offer new possibilities for drug testing. We miniaturized the process of generating hepatic organoids using hiPSC-derived mesenchymal stem cells, hepatocytes, and endothelial cells. These organoids were cultivated in vitro using static and dynamic cultures, and could be maintained for up to 1 week. This hiPSC-derived liver organoid model could potentially replace animal testing in pharmacological and toxicological studies.
Article
Biochemical Research Methods
Bisan Samara, Muhammedin Deliorman, Pavithra Sukumar, Mohammad A. Qasaimeh
Summary: A method of creating cryopreservable high throughput arrays of 3D tumor models using a paper platform is presented, utilizing virtual microwells to facilitate cell aggregation and prevent mixing, allowing for extended storage and drug treatment studies.
Review
Pharmacology & Pharmacy
Greymi Tan, Nicole Ioannou, Essyrose Mathew, Aristides D. Tagalakis, Dimitrios A. Lamprou, Cynthia Yu-Wai-Man
Summary: 3D printing has become increasingly important in healthcare, with applications in drug delivery, organ transplants, and surgical planning. Customized medical devices and implants provide more personalized treatment options for patients.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Review
Health Care Sciences & Services
Mark N. Pernik, Cylaina E. Bird, Jeffrey Traylor, Diana D. Shi, Timothy E. Richardson, Samuel K. McBrayer, Kalil G. Abdullah
Summary: The emergence of patient-derived cancer organoid (PDO) models, which closely recapitulate parental tumor tissue, provides a promising approach in cancer research. PDOs can efficiently retain tumor-specific properties and enhance precision oncologic care for patients compared to traditional cell lines and xenograft models. This review highlights the advantages of PDOs in cancer modeling and potential clinical applications.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Review
Biotechnology & Applied Microbiology
Amanda Marchini, Fabrizio Gelain
Summary: Three-dimensional cell culture systems and organoids are gaining increasing interest for their ability to recreate spatial arrangements and organ functionalities in vitro, providing more relevant and predictive data compared to traditional two-dimensional systems. Scaffold materials play a crucial role in supporting these 3D structures, with synthetic materials showing promising potential due to their customizability and biomimetic properties.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sakthi Lenin, Elise Ponthier, Kaitlin G. Scheer, Erica C. F. Yeo, Melinda N. Tea, Lisa M. Ebert, Mariana Oksdath Mansilla, Santosh Poonnoose, Ulrich Baumgartner, Bryan W. Day, Rebecca J. Ormsby, Stuart M. Pitson, Guillermo A. Gomez
Summary: This study conducted a systematic review on the molecular mechanisms driving glioblastoma progression, identifying 65 drugs/inhibitors for screening their efficacy on patient-derived glioma stem cells. Costunolide, a TERT inhibitor, was found effective in reducing cell viability in both primary and pre-treated tumor models. This novel workflow could lead to personalized and effective treatment for recurrent glioblastoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Stephen D. Clark, Daniel S. Reuland, Alison T. Brenner, Michael P. Pignone
Summary: This study aimed to evaluate the effectiveness of a lung cancer screening decision aid in improving participants' knowledge of screening benefits and risks. Results showed that reducing the chances of death from lung cancer and the risk of diagnosis were the most valued benefits for participants.
Review
Biochemistry & Molecular Biology
Alessandro Posa, Andrea Contegiacomo, Francesca Romana Ponziani, Ernesto Punzi, Giulia Mazza, Annarita Scrofani, Maurizio Pompili, Shraga Nahum Goldberg, Luigi Natale, Antonio Gasbarrini, Evis Sala, Roberto Iezzi
Summary: Personalized cancer treatments aim to provide tailored and biologically driven therapies for cancer patients. Interventional oncology techniques offer locoregional treatment options for malignancies with various mechanisms of action leading to tumor necrosis. The destruction of tumors results in the release of tumor antigens that can stimulate an immune response, which has led to the investigation of combining interventional oncology treatments with immunotherapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
Klaudia Englezos, Lingxin Wang, Edwin C. K. Tan, Lifeng Kang
Summary: The healthcare dynamic has shifted to patient-centred care, requiring more individualised therapies. However, the pharmaceutical industry has not fully adapted to this shift, preventing pharmacists from providing completely personalised medicine to patients. This article reviews the limitations of current pharmaceutical manufacturing methods, discusses the benefits of 3D printing for personalised medicine, and explores the implications for pharmacy practice and policy.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Review
Biotechnology & Applied Microbiology
Arpana Parihar, Vasundhara Pandita, Raju Khan
Summary: This article discusses the use of 3D-printed human organoids for drug screening, as well as their application in personalized medicine for COVID-19. This technology allows for faster and cost-effective drug screening, potentially saving more lives.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Biochemistry & Molecular Biology
Isabella A. Silveira, Adam S. Mullis, Dana M. Cairns, Anna Shevzov-Zebrun, Jordyn Whalen, Alexa Galuppo, Katherine G. Walsh, David L. Kaplan
Summary: This study used 2D and 3D tissue culture models to investigate herpesvirus-induced Alzheimer's Disease (AD). Screening 21 medications, supplements, and nutraceuticals, the researchers found that green tea catechins and resveratrol showed strong anti-plaque properties and neuroprotective benefits, making them potential candidates for further investigation in AD prevention and therapies.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Helmuth Gehart, Johan H. van Es, Karien Hamer, Joep Beumer, Kai Kretzschmar, Johanna F. Dekkers, Anne Rios, Hans Clevers
Editorial Material
Immunology
Kai Kretzschmar, Hans Clevers
SCIENCE IMMUNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Joep Beumer, Jens Puschhof, Julia Bauza-Martinez, Adriana Martinez-Silgado, Rasa Elmentaite, Kylie R. James, Alexander Ross, Delilah Hendriks, Benedetta Artegiani, Georg A. Busslinger, Bas Ponsioen, Amanda Andersson-Rolf, Aurelia Saftien, Charelle Boot, Kai Kretzschmar, Maarten H. Geurts, Yotam E. Bar-Ephraim, Cayetano Pleguezuelos-Manzano, Yorick Post, Harry Begthel, Franka van der Linden, Carmen Lopez-Iglesias, Willine J. van de Wetering, Reinier van der Linden, Peter J. Peters, Albert J. R. Heck, Joachim Goedhart, Hugo Snippert, Matthias Zilbauer, Sarah A. Teichmann, Wei Wu, Hans Clevers
Correction
Biochemistry & Molecular Biology
Joep Beumer, Jens Puschhof, Julia Bauza-Martinez, Adriana Martinez-Silgado, Rasa Elmentaite, Kylie R. James, Alexander Ross, Delilah Hendriks, Benedetta Artegiani, Georg A. Busslinger, Bas Ponsioen, Amanda Andersson-Rolf, Aurelia Saftien, Charelle Boot, Kai Kretzschmar, Maarten H. Geurts, Yotam E. Bar-Ephraim, Cayetano Pleguezuelos-Manzano, Yorick Post, Harry Begthel, Franka van der Linden, Carmen Lopez-Iglesias, Willine J. van de Wetering, Reinier van der Linden, Peter J. Peters, Albert J. R. Heck, Joachim Goedhart, Hugo Snippert, Matthias Zilbauer, Sarah A. Teichmann, Wei Wu, Hans Clevers
Review
Cell Biology
Joris H. Hageman, Maria C. Heinz, Kai Kretzschmar, Jelte van der Vaart, Hans Clevers, Hugo J. G. Snippert
DEVELOPMENTAL CELL
(2020)
Article
Biochemical Research Methods
Else Driehuis, Kai Kretzschmar, Hans Clevers
Article
Multidisciplinary Sciences
Laura C. Demmers, Kai Kretzschmar, Arne Van Hoeck, Yotam E. Bar-Epraim, Henk W. P. van den Toorn, Mandy Koomen, Gijs van Son, Joost van Gorp, Apollo Pronk, Niels Smakman, Edwin Cuppen, Hans Clevers, Albert J. R. Heck, Wei Wu
NATURE COMMUNICATIONS
(2020)
Correction
Biochemical Research Methods
Else Driehuis, Kai Kretzschmar, Hans Clevers
Article
Multidisciplinary Sciences
Anna C. Seubert, Marion Krafft, Kai Kretzschmar
Summary: The oral mucosa is a highly compartmentalized tissue that can be subdivided into multiple parts. Research into the proliferation and differentiation of adult epithelial stem cells has been conducted using in vivo mouse models and organoid technology. Utilizing organoid technology, oral mucosal organoids can be cultured for further study.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Cell & Tissue Engineering
Kai Kretzschmar, Kim E. Boonekamp, Margit Bleijs, Priyanca Asra, Mandy Koomen, Susana M. Chuva de Sousa Lopes, Barbara Giovannone, Hans Clevers
Summary: Research has identified Troy as a marker of epidermal basal cells that play a role in the long-term renewal and cornification of the skin. Single-cell transcriptomics and organoid assays confirmed their stem cell identity and ability to generate differentiating daughter cells.
Article
Biotechnology & Applied Microbiology
Johanna F. Dekkers, Maria Alieva, Astrid Cleven, Farid Keramati, Amber K. L. Wezenaar, Esmee J. van Vliet, Jens Puschhof, Peter Brazda, Inez Johanna, Angelo D. Meringa, Heggert G. Rebel, Maj-Britt Buchholz, Mario Barrera Roman, Amber L. Zeeman, Sam de Blank, Domenico Fasci, Maarten H. Geurts, Annelisa M. Cornel, Else Driehuis, Rosemary Millen, Trudy Straetemans, Mara J. T. Nicolasen, Tineke Aarts-Riemens, Hendrikus C. R. Ariese, Hannah R. Johnson, Ravian L. van Ineveld, Froso Karaiskaki, Oded Kopper, Yotam E. Bar-Ephraim, Kai Kretzschmar, Alexander M. M. Eggermont, Stefan Nierkens, Ellen J. Wehrens, Henk G. Stunnenberg, Hans Clevers, Jurgen Kuball, Zsolt Sebestyen, Anne C. Rios
Summary: This study utilizes a system called BEHAV3D to investigate the dynamic interactions between immune cells and patient cancer organoids. By live-tracking a large number of engineered T cells cultured with patient-derived solid-tumor organoids, a cluster of "super engager" T cells with potent serial killing capacity is identified. The study also examines cancer metabolome-sensing engineered T cells (TEGs) and identifies behavior-specific gene signatures in these cells. Additionally, it demonstrates the ability of type I interferon to prime resistant organoids for TEG-mediated killing.
NATURE BIOTECHNOLOGY
(2022)
Editorial Material
Cell Biology
Delilah Hendriks, Benedetta Artegiani, Kai Kretzschmar
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Annie S. P. Yang, Devanjali Dutta, Kai Kretzschmar, Delilah Hendriks, Jens Puschhof, Huili Hu, Kim E. Boonekamp, Youri van Waardenburg, Susana M. Chuva de Sousa Lopes, Geert-Jan van Gemert, Johannes H. W. de Wilt, Teun Bousema, Hans Clevers, Robert W. Sauerwein
Summary: Researchers have found that hepatocytes derived from human hepatocyte organoids (HepOrgs) can support the development of Plasmodium falciparum, and have identified the importance of the host factor scavenger receptor B1 (SRB1) in parasite development.
NATURE COMMUNICATIONS
(2023)
Review
Immunology
Yotam E. Bar-Ephraim, Kai Kretzschmar, Hans Clevers
NATURE REVIEWS IMMUNOLOGY
(2020)
