Review
Biochemistry & Molecular Biology
Yoichiro Abe, Masato Yasui
Summary: The discovery of NMO-IgG in patients with neuromyelitis optica spectrum disorder (NMOSD) has highlighted the importance of the water channel aquaporin-4 (AQP4) in autoimmune diseases of the central nervous system. The unique structure of AQP4 and its subcellular localization may play a crucial role in the pathogenesis of NMOSD, in addition to the complement-dependent cytotoxic effects of NMO-IgG. Studies have also shown complement-independent cytotoxic effects of NMO-IgG, potentially involving antibody-induced endocytosis of AQP4.
Article
Plant Sciences
Hamza Sohail, Iqra Noor, Muhammad Azher Nawaz, Mingru Ma, Fareeha Shireen, Yuan Huang, Li Yang, Zhilong Bie
Summary: This study characterized the PIP gene family and examined the role of CmoPIP1-4 in salt stress tolerance in pumpkin. The researchers found that CmoPIP1-4 plays a crucial role in salt stress tolerance, but overexpression of these genes also resulted in hypersensitivity to salt. These findings suggest the potential of CmoPIP1-4 as a candidate for developing stress-resistant cucurbit crops.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Chemistry, Physical
Juhee Lee, Yoo Kyung Kang, Eonju Oh, Juhee Jeong, San Hae Im, Duk Ki Kim, Haeshin Lee, Sang-Gyu Kim, Keehoon Jung, Hyun Jung Chung
Summary: The study presents a cancer gene therapy strategy based on NanoRNP that efficiently blocks the PD-L1 immune checkpoint and induces an antitumor effect in vivo without the need for combination therapy. In vivo results demonstrate that NanoRNP can induce indels in target cells at high frequencies, significantly suppressing tumor growth.
CHEMISTRY OF MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Hyo-Ju Jang, Hye-Jeong Park, Hong Seok Choi, Hyun Jun Jung, Tae-Hwan Kwon
Summary: This study aimed to establish mpkCCDc14 cells constitutively expressing V2R and AQP2 via CRISPR/Cas9-mediated genome engineering technology. The results showed that these cells had higher abundance of AQP2 protein in the absence of stimulation and exhibited normal response to dDAVP stimulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Neurosciences
Jenny I. Szu, Devin K. Binder
Summary: This review evaluates the role of AQP4 dysregulation and mislocalization in epilepsy, highlighting their significant impact on neuronal hyperexcitability. Research has shown that the mislocalization of AQP4 can greatly affect the excitability of neurons, playing a crucial role in the development of epilepsy.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2022)
Article
Biochemical Research Methods
Elena Velazquez, Yamal Al-Ramahi, Jonathan Tellechea-Luzardo, Natalio Krasnogor, Victor de Lorenzo
Summary: Research demonstrates that by improving targetron technology, exogenous sequences can be successfully inserted into the genomes of Gram-negative bacteria. Through the establishment of a series of standardized vectors and the combination of CRISPR/Cas9-facilitated counterselection, successful intron-mediated DNA delivery in bacteria was achieved, and P. putida microorganism was successfully tagged.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Ji Liu, Tianli Luo, Yifei Xue, Lanqun Mao, Peter J. Stang, Ming Wang
Summary: The hierarchical self-assembly of metal-organic cages into supramolecular nanoparticles allows for efficient encapsulation of proteins for intracellular delivery and potential therapeutic applications, such as CRISPR/Cas9 genome editing.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biotechnology & Applied Microbiology
Pablo Jimenez-Bonilla, Jun Feng, Shangjun Wang, Jie Zhang, Yifen Wang, David Blersch, Luz Estela de-Bashan, Philippe Gaillard, Liang Guo, Yi Wang
Summary: This study identified key genes responsible for cell autolysis in Clostridium saccharoperbutylacetonicum, providing insights for enhancing strain stability and biofuel production through genome engineering.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sandra Gawlitt, Chunyu Liao, Tatjana Achmedov, Chase L. Beisel
Summary: CRISPR technologies can utilize multiple gRNAs to enact multi-site editing or regulation. Researchers have found that CRISPR arrays utilized by the Cas9 nuclease can be shortened without compromising and sometimes even enhancing targeting activity. Shortened CRISPR-Cas9 arrays can facilitate multiplexed editing and gene regulation from a smaller DNA footprint across many bacterial applications of CRISPR technologies.
Article
Biochemistry & Molecular Biology
Kenta Tomihara, Katsuya Satta, Shohei Matsuzaki, Kazutoshi Yoshitake, Kimiko Yamamoto, Hironobu Uchiyama, Shunsuke Yajima, Ryo Futahashi, Susumu Katsuma, Mizuko Osanai-Futahashi, Takashi Kiuchi
Summary: The researchers identified a gene called Bmmah in silkworm, the knockout of which results in reddish-brown color in eggs and adult compound eyes. Experimental evidence confirmed that Bmmah is responsible for the b-4 phenotype.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Biochemical Research Methods
Ana P. Jacobus, Joneclei A. . Barreto, Lucas S. de Bem, Yasmine A. . Menegon, Icaro Fier, Joao G. R. Bueno, Leandro V. dos Santos, Jeferson Gross
Summary: In this study, a series of EasyGuide plasmids were constructed to simplify CRISPR/Cas9 applications in yeast. By assembling gRNAs in yeast, the experimental workload was significantly reduced, and a highly efficient genome editing procedure was provided.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Virology
Hejin Zhang, Kaiqi Duan, Yingbin Du, Shaobo Xiao, Liurong Fang, Yanrong Zhou
Summary: In this study, a recombinant PRRS virus (rPRRSV) was successfully generated using reverse genetics systems, and a restrictive endonucleases-free method based on CRISPR/Cas9 was developed to edit the PRRSV genome. The study provides a rapid and convenient approach for the development of genetically engineered vaccines against PRRSV and the exploration of PRRSV gene functions.
Article
Biochemical Research Methods
Mohamed Ramadan, Muna Alariqi, Yizan Ma, Yanlong Li, Zhenping Liu, Rui Zhang, Shuangxia Jin, Ling Min, Xianlong Zhang
Summary: By optimizing the CRISPR/Cas9 system, 112 plant development-related genes were successfully knocked out in a single experiment. Using a pooled sgRNAs assembly method, over 800 plants were generated, all of which covered the targeted genes. Different sgRNA insertions resulted in various combinations of phenotypes in plant tissues.
Article
Agronomy
Leyuan Tao, Bing Wang, Shichao Xin, Wei Li, Shengcai Huang, Laihua Liu, Jing Cui, Qianru Zhang, Xianguo Cheng
Summary: In this study, the editing of OsPIP1 genes in rice using CRISPR/Cas9 system revealed that functional loss of OsPIP1 genes leads to delayed growth and compromised salt stress tolerance in rice. The editing also affected ion balance and osmotic regulation in the roots of rice plants.
Article
Veterinary Sciences
Quan Xie, Shiya Cao, Wei Zhang, Weikang Wang, Luyuan Li, Qiuqi Kan, Hui Fu, Tuoyu Geng, Tuofan Li, Zhimin Wan, Wei Gao, Hongxia Shao, Aijian Qin, Jianqiang Ye
Summary: This study developed a recombinant virus FA4-EGFP expressing EGFP-Fiber-2 fusion protein using the CRISPR/Cas9 technique, which showed significant attenuation compared to wild type FAdV-4 and provided protection against lethal challenge in chickens. Additionally, FA4-EGFP induced neutralizing antibodies with higher titers earlier than an inactivated vaccine, suggesting its potential as a live-attenuated vaccine candidate against highly pathogenic FAdV-4.
VETERINARY RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Claudia Palazzo, Pasqua Abbrescia, Onofrio Valente, Grazia Paola Nicchia, Shervin Banitalebi, Mahmood Amiry-Moghaddam, Maria Trojano, Antonio Frigeri
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Eloisa Sardella, Maria Grazia Mola, Roberto Gristina, Monica Piccione, Valeria Veronico, Manuela De Bellis, Antonio Cibelli, Maura Buttiglione, Vincenza Armenise, Pietro Favia, Grazia Paola Nicchia
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Pharmacology & Pharmacy
Elena Conte, Adriano Fonzino, Antonio Cibelli, Vito De Benedictis, Paola Imbrici, Grazia Paola Nicchia, Sabata Pierno, Giulia Maria Camerino
FRONTIERS IN PHARMACOLOGY
(2020)
Article
Neurosciences
Antonio Cibelli, Sandra Veronica Lopez-Quintero, Sean Mccutcheon, Eliana Scemes, David C. Spray, Randy F. Stout, Sylvia O. Suadicani, Mia M. Thi, Marcia Urban-Maldonado
Summary: The study successfully transduced mouse cortical astrocytes using a lentiviral vector encoding hTERT, generating immortalized cell lines with characteristics consistent with neonatal astrocytes. These cell lines showed stability in the expression of astrocyte-specific markers over cell passages.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Neurosciences
Francesco Pisani, Laura Simone, Maria Grazia Mola, Manuela De Bellis, Antonio Frigeri, Grazia Paola Nicchia, Maria Svelto
Summary: In astrocytes, unknown mechanisms regulate the expression of M1 and M23 isoforms of water channel aquaporin-4. This study found that deletion of M23-AQP4 in mouse spinal cord alters the interaction between AQP4 mRNA and some RBPs involved in AQP4 translation. The RNA helicase DDX17 was identified as a regulator of AQP4 expression in astrocytes.
Article
Multidisciplinary Sciences
David O. Dias, Jannis Kalkitsas, Yildiz Kelahmetoglu, Cynthia P. Estrada, Jemal Tatarishvili, Daniel Holl, Linda Jansson, Shervin Banitalebi, Mahmood Amiry-Moghaddam, Aurelie Ernst, Hagen B. Huttner, Zaal Kokaia, Olle Lindvall, Lou Brundin, Jonas Frisen, Christian Goritz
Summary: The study reveals that fibrotic scarring is common in mice and humans following central nervous system lesions, deriving from a distinct population of GLAST-expressing perivascular cells.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Antonio Cibelli, Randy Stout, Aline Timmermann, Laura de Menezes, Peng Guo, Karen Maass, Gerald Seifert, Christian Steinhaeuser, David C. Spray, Eliana Scemes
Summary: The neurovascular unit consists of endothelial cells, pericytes, and astrocytes, with connexin 43 (Cx43) and Cx30 forming gap junctions in astrocyte endfeet, influencing blood-brain barrier integrity. The study shows that the distribution and mobility of gap junction proteins in astrocyte endfeet impact the organization of the unit and BBB integrity.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Ross Luu, Silvana Valdebenito, Eliana Scemes, Antonio Cibelli, David C. Spray, Maximiliano Rovegno, Juan Tichauer, Andrea Cottignies-Calamarte, Arielle Rosenberg, Calude Capron, Sandrine Belouzard, Jean Dubuisson, Djillali Annane, Geoffroy Lorin de la Grandmaison, Elisabeth Cramer-Borde, Morgane Bomsel, Eliseo Eugenin
Summary: The study found that SARS-CoV-2 induces a more significant and prolonged opening of Panx-1 channels compared to other viruses, leading to increased release of ATP, PGE(2), and IL-1 beta. Therefore, the Panx-1 channel is considered a critical factor in SARS-CoV-2 infection and could be a potential alternative therapy.
Article
Cell Biology
Antonio Cibelli, Eliana Scemes, David C. Spray
Summary: Cholesterol levels play a crucial role in the distribution and function of Pannexin1 channels in neural cells. Manipulation of cholesterol levels could potentially modulate various pathological conditions through the activation of Pannexin1 channels.
Review
Biochemistry & Molecular Biology
Giacomina Brunetti, Barbara Barile, Grazia Paola Nicchia, Francesco Onorati, Giovanni Battista Luciani, Antonella Galeone
Summary: ST2 is a member of the interleukin 1 receptor family and has soluble sST2 and transmembrane ST2L isoforms. The ligand of ST2 is IL-33, which activates intracytoplasmic mediators through binding with ST2L and IL-1RAcP, leading to nuclear signal and cardiovascular effects. Conversely, sST2 is released in the blood and acts as a decoy receptor, binding IL-33 and blocking IL-33/ST2L interaction. sST2 is mainly involved in maintaining homeostasis and/or tissue alterations, as the balance/activation of the IL-33/ST2L axis plays a role in fibrosis, tissue damage, inflammation, and remodeling.
Article
Biochemistry & Molecular Biology
Shervin Banitalebi, Nadia Skauli, Samuel Geiseler, Ole Petter Ottersen, Mahmood Amiry-Moghaddam
Summary: This study investigates the mechanisms of scar formation in the brain and finds pronounced changes in the astrocytic protein AQP4 in the infarct border zone, which may be related to the downregulation of the AQP4 isoform AQP4ex. These findings provide new insights into the molecular basis of scar formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)