Article
Plant Sciences
Samuel Gamez-Arcas, Francisco Jose Munoz, Adriana Ricarte-Bermejo, Angela Maria Sanchez-Lopez, Marouane Baslam, Edurne Baroja-Fernandez, Abdellatif Bahaji, Goizeder Almagro, Nuria De Diego, Karel Dolezal, Ondrej Novak, Jesus Leal-Lopez, Rafael Jorge Leon Morcillo, Araceli G. Castillo, Javier Pozueta-Romero
Summary: The response to microbial volatile compounds (VCs) in Arabidopsis involves the action of glucose-6-phosphate/phosphate translocator2 (GPT2) rather than phosphoglucose isomerase1 (PGI1), promoting growth, photosynthesis, and starch accumulation. Overexpression of GPT2 controlled by a vascular tissue- and root tip-specific promoter resets the photosynthesis-related proteome in leaves.
Article
Biochemistry & Molecular Biology
Nicole Swope, Katherine E. Lake, Golda H. Barrow, Daniel Yu, Daniel A. Fox, Linda Columbus
Summary: The article discusses the function and catalytic mechanism of phosphoglucose isomerase TmPGI. Experimental results show that TmPGI proceeds through a cis-enediol-based mechanism and E281 is a key amino acid residue, with H310 or K422 potentially facilitating the catalysis.
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2021)
Article
Biochemistry & Molecular Biology
R. W. Meek, I. T. Cadby, A. L. Lovering
Summary: Glycolysis and gluconeogenesis are central metabolic pathways in all domains of life. The enzyme phosphoglucose isomerase (PGI) plays a crucial role in these pathways, with an induced-fit conformational change within the active site not always necessary for substrate binding in some PGIs. The B. bacteriovorus PGI enzyme, characterized by a phenylalanine residue substitution, represents a minimal functional PGI.
Article
Plant Sciences
Fei Gao, Huijun Zhang, Wenjuan Zhang, Ning Wang, Shijia Zhang, Chengcai Chu, Cuimin Liu
Summary: Starch plays a crucial role in plant carbon assimilation and energy storage, with phosphoglucose isomerase (PGI) influencing starch synthesis and plant growth. Manipulating PGI activity can lead to increased plant biomass and yield productivity.
Article
Multidisciplinary Sciences
Carlos Anerillas, Allison Herman, Rachel Munk, Amanda Garrido, Kwan-Wood Gabriel Lam, Matthew J. Payea, Martina Rossi, Dimitrios Tsitsipatis, Jennifer L. Martindale, Yulan Piao, Krystyna Mazan-Mamczarz, Jinshui Fan, Chang-Yi Cui, Supriyo De, Kotb Abdelmohsen, Rafael de Cabo, Myriam Gorospe
Summary: This study demonstrates that BDNF enhances the viability of senescent cells through TrkB activation, and inhibition of TrkB can reduce the accumulation of senescent cells in aged mouse organs.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Yao Zhou, Chao Du, Arome Solomon Odiba, Rui He, Chukwuemeka Samson Ahamefule, Bin Wang, Cheng Jin, Wenxia Fang
Summary: The phosphoglucose isomerase (PGI) plays a crucial role in maintaining sugar homeostasis, stress response, and pathogenicity in Aspergillus flavus. Deletion of PGI results in specific carbon requirement for survival, reduced conidiation, slowed germination, hypersusceptibility to various stresses, and significant attenuated virulence. PGI could be a potential target for controlling infection and aflatoxin contamination caused by A. flavus.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nam Ho Jeoung, Ae Lim Jo, Hee Sung Park
Summary: AMF from different cancer cells can suppress breast cancer cell growth, with AMF from AsPC-1 pancreatic cancer cells showing stronger inhibitory activity. AsPC-1:AMF can arrest breast cancer cells by regulating multiple signaling pathways. Additionally, AsPC-1:AMF and methyl jasmonate (MJ) cooperatively act against breast cancer cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Chemistry, Physical
Timothy A. Coulther, Jaeju Ko, Mary Jo Ondrechen
Summary: Interactions between catalytic and neighboring amino acids can increase catalytic rates by elongating the buffer range and enhancing coupling of protonation equilibria. Differences in intrinsic pK(a)s and energy of interaction between residues play a key role in optimizing this coupling for efficient catalysis.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Food Science & Technology
Seok Joong Kim, Yangji Kim, Thanh Van Duong, Hee Sung Park
Summary: Research has revealed the inhibitory effect of autocrine motility factor (AMF) on colorectal cancer (CRC) cell proliferation, achieved by downregulating the expression of glucose-6-phosphate dehydrogenase (G6PD) and increasing the generation of reactive oxygen species (ROS). The combination of D-AMF and curcumin proved highly effective in eliminating curcumin-resistant CRC cells.
APPLIED BIOLOGICAL CHEMISTRY
(2023)
Article
Immunology
Abhinandan Chowdhury, Matthew R. Lewin, Christina N. Zdenek, Rebecca Carter, Bryan G. Fry
Summary: African spitting cobras have potent anticoagulant venom activity due to strong inhibition of Factor Xa by venom phospholipase A(2) toxins, which current antivenoms cannot neutralize. Research has expanded this trait to include more cobras and found that enzyme-inhibitors can effectively counteract the toxic effects. This suggests potential alternative treatments for snakebite envenoming beyond traditional antivenoms.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Elitsa Boteva, Konstantin Doychev, Rositsa Tsekovska, Yordan Handzhyski, Ivan Ivanov, Roumyana Mironova
Summary: The Maillard reaction is a non-enzymatic reaction that affects proteins and DNA in organisms. Recent studies have shown that the enzyme PGI in Escherichia coli can catalyze the deglycation of DNA modified with Amadori products. In this study, we tested the deglycation activity of PGI on a model protein, glycated lysozyme, and found that PGI had higher affinity but lower catalytic constant compared to the free substrate.
COMPTES RENDUS DE L ACADEMIE BULGARE DES SCIENCES
(2023)
Article
Microbiology
Yao Zhou, Kaizhou Yan, Qijian Qin, Olawale G. Raimi, Chao Du, Bin Wang, Chukwuemeka Samson Ahamefule, Bartosz Kowalski, Cheng Jin, Daan M. F. van Aalten, Wenxia Fang
Summary: Aspergillus fumigatus is a deadly opportunistic fungal pathogen causing hundreds of thousands of deaths every year. Our study reveals that phosphoglucose isomerase (PGI) plays an important role in the growth, cell wall biosynthesis, and virulence of A. fumigatus. The crystal structure of PGI provides a foundation for the development of PGI as a potential antifungal target.
Review
Biotechnology & Applied Microbiology
Renan Yuji Miyamoto, Ricardo Rodrigues de Melo, Isabelle Lobo de Mesquita Sampaio, Amanda Silva de Sousa, Edvaldo Rodrigo Morais, Cintia Regina Sargo, Leticia Maria Zanphorlin
Summary: Isomerases, particularly xylose isomerases (XIs), are enzymes widely studied for their role in industrial processes such as biotransforming xylose. The application of XIs in downstream metabolism of microorganisms is a smart strategy for producing value-added biochemicals with broad industrial applications. Recent advancements in XIs have expanded their range of applications, providing comprehensive insights into their mechanisms of action and potential for developing xylose bioprocesses.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Article
Medicine, Research & Experimental
Mohamed E. Shaker, Nada H. Eisa, Abdelaziz Elgaml, Ahmed El-Mesery, Mohamed El-Shafey, Mohamed El-Dosoky, Mohammed El-Mowafy, Mohamed El-Mesery
Summary: Mannose significantly alleviated TAA-induced liver fibrosis by reducing fibrotic bridges, limiting hepatocellular injury, reversing oxidative stress and proinflammatory cytokine release, inhibiting hepatic stellate cells activation, among other mechanisms.
Article
Entomology
Jianing Yang, Di Wang, Hui Liu, Lin Wang, Ling Jin, Virpi Ahola, Chongren Xu, Rongjiang Wang
Summary: Temperature is a crucial environmental factor that affects organisms, particularly ectotherms, through its impact on protein stability. This study investigates the amino acid substitutions in phosphoglucose isomerase (PGI) that are related to thermostability in the Glanville fritillary butterfly. The results show that specific amino acid substitutions enhance PGI's thermostability, which increases the interaction between PGI monomers and stabilizes the protein's 3D structure. Furthermore, this adaptation strategy through amino acid substitutions is also observed in other butterfly species in the same region, suggesting convergent evolution.
Article
Chemistry, Multidisciplinary
Marion Devillers, Jean-Philip Piquemal, Laurent Salmon, Nohad Gresh
JOURNAL OF COMPUTATIONAL CHEMISTRY
(2020)
Article
Materials Science, Biomaterials
Rabih Khoder, Hafsa Korri-Youssoufi
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2020)
Article
Chemistry, Analytical
Zouhour Hsine, Salma Bizid, Rym Mlika, Helene Sauriat-Dorizon, Ayoub Haj Said, Hafsa Korri-Youssoufi
Article
Biochemical Research Methods
Zouhour Hsine, Saber Blili, Rym Milka, Helene Dorizon, Ayoub Haj Said, Hafsa Korri-Youssoufi
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2020)
Article
Nanoscience & Nanotechnology
Nada Elgiddawy, Shiwei Ren, Abderrahim Yassar, Alain Louis-Joseph, Helene Sauriat-Dorizon, Waleed M. A. El Rouby, Ahmed O. El-Gendy, Ahmed A. Farghali, Hafsa Korri-Youssoufi
ACS APPLIED MATERIALS & INTERFACES
(2020)
Article
Electrochemistry
Yaqiong Wang, Zouhour Hsine, Helene Sauriat-Dorizon, Rym Mlika, Hafsa Korri-Youssoufi
ELECTROCHIMICA ACTA
(2020)
Article
Materials Science, Multidisciplinary
Meryem Bouaziz, Wei Zhang, Yongfeng Tong, Hamid Oughaddou, Hanna Enriquez, Rym Mlika, Hafsa Korri-Youssoufi, Zhesheng Chen, Heqi Xiong, Yingchun Cheng, Azzedine Bendounan
Summary: In this study, the structural and electronic properties of Au(111) surface after Te deposition in ultrahigh vacuum were investigated. The results showed different surface reconstructions and potential formation of an Au-Te alloy structure at different Te film thicknesses.
Review
Chemistry, Analytical
Maliana El Aamri, Ghita Yammouri, Hasna Mohammadi, Aziz Amine, Hafsa Korri-Youssoufi
Article
Chemistry, Analytical
Nada Elgiddawy, Shiwei Ren, Wadih Ghattas, Waleed M. A. El Rouby, Ahmed O. El-Gendy, Ahmed A. Farghali, Abderrahim Yassar, Hafsa Korri-Youssoufi
Summary: In this study, stable CTAB-P3HT NPs were prepared using a simple mini-emulsion method and characterized using various techniques. The NPs exhibited excellent fluorescence properties and electroactivity, making them suitable for bacterial detection and antimicrobial activity.
Article
Chemistry, Multidisciplinary
Maroua Hamami, Noureddine Raouafi, Hafsa Korri-Youssoufi
Summary: This study presents a biosensor platform based on DNA aptamers for detecting the agricultural pesticide acetamiprid, with stable capacitance performance and high selectivity. By combining DNA aptamers with MoS2 nanosheets, the detection of acetamiprid and ion diffusion are facilitated.
APPLIED SCIENCES-BASEL
(2021)
Article
Chemistry, Analytical
Maroua Hamami, Meryem Bouaziz, Noureddine Raouafi, Azzedine Bendounan, Hafsa Korri-Youssoufi
Summary: The study introduces an electrochemical aptasensor for the detection of ampicillin, utilizing a nanostructure composed of MoS2 nanosheets and conductive polypyrrole nanoparticles. By studying the structural and morphological properties of the nanocomposite, it was found that the material formed a synergetic effect on the electrochemical redox process. The biosensor demonstrated high sensitivity and selectivity, with a limit of detection of 10 pg/L for ampicillin, making it a promising device for environmental antibiotic detection.
Review
Chemistry, Analytical
Zouhour Hsine, Rym Mlika, Nicole Jaffrezic-Renault, Hafsa Korri-Youssoufi
Summary: Graphene and its derivatives have been widely used for electrochemical detection of dopamine due to their high surface area and good conductivity. Modified graphene and graphene-based nanocomposites show improved catalytic activity for dopamine detection. This review summarizes recent advances in graphene-based electrodes for electrochemical detection of dopamine and highlights the advantages of using polymers as linker platforms to form graphene-based nanocomposites for electrochemical dopamine sensors.
Article
Chemistry, Analytical
Rabiaa Helim, Ali Zazoua, Nicole Jaffrezic-Renault, Hafsa Korri-Youssoufi
Summary: We aimed to develop an electrochemical sensor for detecting the highly toxic water contaminant divalent metal ion (lead II). A sensor was formed using hemicellulose polysaccharide extracted from the Opuntia Ficus Indica cactus associated with agarose as a sensitive layer on a gold electrode. The sensor demonstrated high sensitivity towards Pb2+ with a detection limit of 1.3 fM, showing a potential application for lead detection in river and sea water.
Correction
Biochemistry & Molecular Biology
Mohamed Marzouk, Shimaa M. Khalifa, Amal H. Ahmed, Ahmed M. Metwaly, Hala Sh. Mohammed, Hanan A. A. Taie
BIOORGANIC CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Gerardo Andres Libreros-Zuniga, Danilo Pava e Pavao, Vinicius de Morais Barroso, Nathalya Cristina de Moraes Roso Mesquita, Saulo Fehelberg Pinto Braga, Glaucius Oliva, Rafaela Salgado Ferreira, Kelly Ishida, Marcio Vinicius Bertacine Dias
Summary: Tuberculosis is a major global cause of death, and the emergence of drug-resistant strains has increased the burden of this disease. New alternative therapies are constantly needed, and recent research has identified small molecules as potential inhibitors of Ldts in M. tuberculosis, which have antimycobacterial activity.
BIOORGANIC CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Xiao-Dong Wang, Yong-Si Liu, Ming-Hao Hu
Summary: In this study, a selffolded fluorescent probe was designed to selectively illuminate G4s by unfolding its intramolecular aggregation mediated by G4 binding. This probe showed more controllable background emission and promising ability to track G4 forming dynamics compared to previous disaggregation-induced emission (DIE) probes.
BIOORGANIC CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Yu Xiang, Zhuo Yuan, Qichuan Deng, Linshen Xie, Dongke Yu, Jianyou Shi
Summary: This review provides a brief description of the diagnosis, pathogenesis, and potential therapeutic inhibitors for renal fibrosis. Currently, there are no clear therapeutic targets or drugs for renal fibrosis; however, some natural products may have potential efficacy for treating renal fibrosis.
BIOORGANIC CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Simone Giovannuzzi, Anna Nikitjuka, Bruna Rafaela Pereira Resende, Michael Smietana, Alessio Nocentini, Claudiu T. Supuran, Jean-Yves Winum
Summary: Boron-based compounds have been extensively studied in medicinal chemistry, playing a crucial role in designing small molecule drugs for various diseases. Boron is particularly valuable in developing inhibitors for metalloenzymes carbonic anhydrases, and it can modulate ligand recognition ability and selectivity. Recent advancements have led to the discovery of novel boron-based inhibitors that can inhibit carbonic anhydrases through a Lewis acid-base mechanism. Further research is needed to fully explore the potential of boron-based inhibitors and advance their clinical applications.
BIOORGANIC CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Xinxin Liu, Lei Chen, Ze Chen
Summary: This study developed a nanostructured photosensitizer loaded with oxygen-throttling drug and demonstrated its enhanced cytotoxicity against tumor cells under hypoxic conditions. Animal experiments showed the enhanced tumor targeting capability of the photosensitizer and its inhibitory effect on tumor growth.
BIOORGANIC CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Shuai Jiang, Wen-Yan Li, Zai-Feng Yuan, Qin-Shi Zhao
Summary: This study isolated two new dimeric Lycopodium alkaloids and twelve previously undescribed Lycopodium alkaloids from Lycopodiastrum casuarinoides. The structures of these compounds were determined and their inhibitory activities on the Cav3.1 channel were evaluated.
BIOORGANIC CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Yan Yang, Dong-Xiao Yan, Rui-Xue Rong, Bing-Ye Shi, Man Zhang, Jing Liu, Jie Xin, Tao Xu, Wen-Jie Ma, Xiao-Liu Li, Ke-Rang Wang
Summary: In this study, a series of nucleolar fluorescent probes based on naphthalimide derivatives were designed and synthesized, which could achieve clear nucleolar staining in living cells. The results showed that these probes exhibited good targeting to the cell nucleolus and could bind to RNA and enhance fluorescence. This has positive implications for the diagnosis and treatment of nucleolus-related diseases.
BIOORGANIC CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Yongxi Dong, Fang Wang, Jinlan Wen, Yongqing Mao, Shanhui Zhang, Tiemei Long, Zhangxiang Yang, Lei Li, Jiquan Zhang, Li Dong, Gang Liu, Jianwei Xu
Summary: The hybrid molecules of Scutellarein and Tertramethylpyrazine show excellent neuroprotective and antiplatelet effects in the treatment of ischemic stroke. Compound 1e is particularly effective, enhancing cell membrane permeability and inhibiting cell uptake, as well as significantly reducing cerebral infarction volume.
BIOORGANIC CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Yu Chen, Yuanyuan Ying, Jonathan Lalsiamthara, Yuheng Zhao, Saber Imani, Xin Li, Sijing Liu, Qingjing Wang
Summary: This paper examines the role and metabolic regulation of NAD+ in bacteria, highlighting its impact on physiology and virulence. It explores enzymes associated with NAD+ metabolism as potential targets for antibacterial drugs and vaccine candidates. Additionally, it scrutinizes the medical potential of NAD+ and provides insights for its application in biomedicine.
BIOORGANIC CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Jon Macicior, Daniel Fernandez, Silvia Ortega-Gutierrez
Summary: Hutchinson-Gilford progeria syndrome (HGPS), also known as progeria, is a rare genetic disease that causes premature aging and significantly reduces life expectancy. Currently, there is only one approved drug for treating progeria, but its efficacy is limited. Progerin levels are believed to be the most important biomarker related to disease severity.
BIOORGANIC CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Fuko Hirano, Naoya Kondo, Yusuke Murata, Aya Sudani, Takashi Temma
Summary: Fluorinated alpha-methyl 3BPA derivatives showed improved water solubility, tumor targetability, and biodistribution compared to 3BPA and BPA, resulting in significantly improved tumor-to-normal tissue ratios.
BIOORGANIC CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Ying Shi, Jiaqin Tang, Shumeng Zhi, Ruiqi Jiang, Qing Huang, Lei Sun, Zhizhong Wang, Yanran Wu
Summary: Necroptosis is a type of cell death associated with various diseases. In this study, we identified a small molecule inhibitor, SY-1, that effectively blocks necroptosis by inhibiting the phosphorylation of RIP1/RIP3/MLKL pathway. SY-1 also showed protective effects against TNF-induced hypothermia and improved survival in mice with SIRS. These findings highlight the potential therapeutic applications of SY-1.
BIOORGANIC CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Andrea Bagan, Sonia Abas, Judith Pala-Pujadas, Alba Irisarri, Christian Grinan-Ferre, Merce Pallas, Itziar Muneta-Arrate, Carolina Muguruza, Luis F. Callado, Belen Perez, Elies Molins, Jose A. Morales-Garcia, Carmen Escolano
Summary: Recent studies have identified the modulation of imidazoline I-2 receptors (I-2-IR) by selective ligands as a potential strategy for treating neurodegenerative diseases. This study reports a family of bicyclic alpha-iminophosphonates that show high affinity and selectivity for I-2-IR and demonstrates their neuroprotective and anti-inflammatory effects in in vitro and in vivo models. The findings emphasize the importance of exploring structurally novel I-2-IR ligands for therapeutic strategies in neurodegeneration.
BIOORGANIC CHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Qiuping Xiang, Tianbang Wu, Cheng Zhang, Chao Wang, Hongrui Xu, Qingqing Hu, Jiankang Hu, Guolong Luo, Xiaoxi Zhuang, Xishan Wu, Yan Zhang, Yong Xu
Summary: This study reports the discovery of a 1-(indolizin-3-yl)ethan-1-one derivative as a potent and selective CBP bromodomain inhibitor for AML drug development.
BIOORGANIC CHEMISTRY
(2024)