Article
Chemistry, Multidisciplinary
Jia Liu, Wei Ha, Eshbakova Komila Alibekovna, Rui Ma, Yan-Ping Shi
Summary: Abnormal expression of acetylcholinesterase (AChE) is related to Alzheimer's disease (AD). In this study, a platform of ruptured organosilica nanocapsules (RONs) immobilized AChE coupled with an MnO2-OPD colorimetric assay was developed to monitor AChE activity and screen AChE inhibitors. The results showed that beta-cyclocostunolide from Inula macrophylla exhibited the best AChE inhibitory activity.
Article
Physics, Applied
Yuji Nakagawa, Masayuki Takagishi, Naoyuki Narita, Tazumi Nagasawa, Gaku Koizumi, Wenyu Chen, Shohei Kawasaki, Tetsuya Roppongi, Akihiko Takeo, Tomoyuki Maeda
Summary: This study demonstrates the coupled out-of-plane oscillation between two oscillation layers within a spin-torque oscillator (STO). The characteristics of the coupled oscillation state and its degradation due to the mixing of the free oscillation state depending on the bias current are analyzed. The behaviors are described analytically and reproduced numerically based on the Landau-Lifshitz-Gilbert equation. The coupled oscillation state and the subsequent microwave magnetic field can be applied for microwave-assisted magnetic recording for high-density recording as STOs are fabricated in the write head of a hard disk drive.
APPLIED PHYSICS LETTERS
(2023)
Article
Chemistry, Analytical
Qingnan Li, Wenzhi Qiang, Jie Yuan, Lehui Xiao
Summary: The sensitive and accurate detection of biomarkers is crucial in clinical diagnosis and drug discovery. Existing amplification-based methods face challenges of limited reproducibility and high background noise. To solve these problems, a robust plasmonic nanoparticle-coupled single-molecule kinetic fingerprinting (PNP-SMKF) method is developed, allowing ultrasensitive detection of protein kinase A (PKA). This method shows great potential as a versatile platform for enzyme detection and inhibitor screening.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Tomas Mikysek, Michaela Fruhbauerova, Ivan Svancara, Miroslav Novak, Milan Sys
Summary: A new voltammetric method is presented for determining the kinetic parameters of artificial metalloenzymes. This method offers a promising alternative to the commonly used spectrophotometric method.
Article
Biochemical Research Methods
Limeng Cai, Yuqi Wang, Ying Yang, Hao Wu
Summary: This study demonstrates a convenient, fast, and low-cost assay for quantifying glycolysis rate in small amount of cells. The assay showed good sensitivity and stability and could be applied in cancerous and immunological research.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
F. Peter Guengerich, Stella A. Child, Ian R. Barckhausen, Margo H. Goldfarb
Summary: The bacterial cytochrome P450(cam) binds its substrate camphor through an induced-fit mechanism, where the enzyme changes conformation after binding the substrate. This behavior is different from other P450 enzymes that utilize a conformational selection process for substrate recognition. The accessory protein putidaredoxin has no effect on substrate binding, indicating a unique mechanism for substrate recognition by P450(cam).
Article
Chemistry, Multidisciplinary
Mirko Lohse, Manuel W. Thesen, Anja Haase, Martin Smolka, Nerea Briz Iceta, Ana Ayerdi Izquierdo, Isbaal Ramos, Clarisa Salado, Arne Schleunitz
Summary: This study presents the fabrication process of a novel network of micron-sized channels using UV-nanoimprint lithography, along with an evaluation of a suitable UV-curable material. The results suggest that the given pattern, in combination with the resist, can lead to faster, cheaper, and more reliable drug screening.
Article
Cardiac & Cardiovascular Systems
Juliette Hall, Kate M. Bourne, Steven Vernino, Viktor Hamrefors, Isabella Kharraziha, Jan Nilsson, Robert S. Sheldon, Artur Fedorowski, Satish R. Raj
Summary: This study compared concentrations of autoantibodies to cardiovascular G protein-coupled receptors between patients with POTS and healthy controls, and found no significant differences. These findings suggest that these tests are not useful for establishing the role of autoimmunity in POTS.
Article
Multidisciplinary Sciences
Lena A. K. Boegeholz, Evan Mercier, Wolfgang Wintermeyer, Marina V. Rodnina
Summary: Protein synthesis in bacteria starts with a formylated methionine on the ribosome, with removal essential for further processing. Peptide deformylase (PDF) quickly binds to ribosome-bound nascent-chains for efficient scanning of substrates. The rate-limiting step in the PDF mechanism is a conformational rearrangement of the nascent-chain post formyl group cleavage, with PDF also potentially acting as an early chaperone.
SCIENTIFIC REPORTS
(2021)
Article
Food Science & Technology
Kunal Garg, Fausto Villavicencio-Aguilar, Flora Solano-Rivera, Leona Gilbert
Summary: A direct competitive ELISA was developed to detect multiple mycotoxins in human serum with low limits of quantitation and high recovery rates. The assay demonstrated excellent accuracy, precision, and specificity, making it a reliable method for mycotoxin exposure assessment in humans.
Article
Agricultural Engineering
Shazia Rehman, Yvette Shihui Yang, Raffel Dharma Patria, Talha Zulfiqar, Noman Khalid Khanzada, Rabia Jalil Khan, Carol Sze Ki Lin, Duu-Jong Lee, Shao-Yuan Leu
Summary: Food waste biorefinery is a sustainable method for producing green chemicals, but the factors hindering enzymatic hydrolysis efficiency have not been clear. This study investigated the effects of different cooking and storing methods on food waste and determined the optimal dosages and reaction coefficients. Drying and frying decreased the hydrolysis rate, while hydrothermal pretreatment increased it.
BIORESOURCE TECHNOLOGY
(2023)
Article
Thermodynamics
Chenjun Liu, Wei Cao, Xuding Song, Yipin Wan
Summary: With the development of picosecond laser technology, this paper establishes a three-dimensional dual-phase-lagging heat transfer model and provides a new approach to solve the problem of three-dimensional dual-phase-lagging heat transfer. This semianalytical method shows high accuracy and significant impact of phase lag constants on the temperature field, which can be explained by using the two-step heat transfer theory.
INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER
(2024)
Article
Chemistry, Multidisciplinary
Li-Jun Ouyang, Bing-Qing Xie, Bin Ding
Summary: This paper investigates the approximate lag synchronization and anticipating synchronization between two unidirectionally coupled hyperchaotic Chen systems. The synchronization condition for exact lag synchronization in systems with time delay in signal transmission is obtained, and approximate lag synchronization and anticipating synchronization are discussed by replacing the true time-delay terms. The study demonstrates a new method for recreating past signals or predicting future signals using current signals, and provides a simple way to eliminate the negative effects of time delay in signal transmission.
APPLIED SCIENCES-BASEL
(2023)
Article
Engineering, Electrical & Electronic
Chua-Chin Wang, Pang-Yen Lou, Tsung-Yi Tsai, I-Yu Huang, Yu-Cheng Lin, Tzung-Je Lee, Guan-Ru Chen
Summary: This study proposes a high-accuracy impedance read-out circuit implemented using a TSMC 0.18 um mixed-signal CMOS process for BIA sensors to detect biomarkers. By utilizing a high-order approximation model and incorporating operational amplifiers for noise rejection, the system demonstrates high accuracy with a maximum impedance error of less than 6% and a phase error of less than 3 degrees in a frequency range from 1 kHz to 2 MHz.
CIRCUITS SYSTEMS AND SIGNAL PROCESSING
(2021)
Article
Biochemical Research Methods
Ruben R. G. Soares, Ahmad S. Akhtar, Ines F. Pinto, Noa Lapins, Donal Barrett, Gustaf Sandh, Xiushan Yin, Vicent Pelechano, Aman Russom
Summary: This study aimed to develop a cost-effective viral load detection system for point-of-care COVID-19 diagnostics. By developing an integrated modular centrifugal microfluidic platform, rapid amplification and detection of viral RNA from nasopharyngeal swab samples with high specificity and sensitivity were achieved.
Article
Engineering, Electrical & Electronic
Mihir Gupta, Sybren Santermans, Bert Du Bois, Rita Vos, Simone Severi, Geert Hellings, Liesbet Lagae, Koen Martens, Willem Van Roy
IEEE SENSORS JOURNAL
(2020)
Article
Engineering, Electrical & Electronic
Mihir Gupta, Sybren Santermans, Geert Hellings, Liesbet Lagae, Koen Martens, Willem Van Roy
Summary: The experimental results show that controlling the surface total charge and modulating the local ion concentration can alter the sensitivity of FET to charged molecules, with excess screening leading to a decrease in sensitivity.
IEEE SENSORS JOURNAL
(2021)
Article
Biochemical Research Methods
P. Neutens, R. Jansen, G. Woronoff, M. Rutowska, N. Hosseini, F. Buja, A. Humbert, F. Colle, T. Stakenborg, W. Van Roy
Summary: This paper presents a miniaturized waveguide-based absorption measurement system operating at a wavelength of 635 nm, based on a silicon nitride integrated photonic platform, suitable for lab-on-chip applications. Experimental results demonstrate a high correlation between the bulk dye concentration and the measured absorption loss levels in the waveguides. A photonic design process is explained to choose the ideal waveguide for minimizing the coefficient of variation on the analyte concentration, designed for camera readout and easy integration for lab-on-chip cartridge approach.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Biophysics
Olivier Tytgat, Maarten Fauvart, Tim Stakenborg, Dieter Deforce, Filip Van Nieuwerburgh
Summary: The demand for forensic DNA profiling is increasing, leading to challenges in decentralized workflow due to the need for bulky equipment. Researchers developed a novel hybridization-based STR genotyping method using STRide probes, showing high sensitivity and potential for on-site forensic DNA fingerprinting.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Pharmacology & Pharmacy
Antoine D'Hollander, Ruben Van Roosbroeck, Jesse Trekker, Tim Stakenborg, Tom Dresselaers, Greetje Vande Velde, Tom Struys, Ivo Lambrichts, Jeroen Lammertyn, Liesbet Lagae, Uwe Himmelreich
Summary: The use of synthetic antiferromagnetic nanoparticles (SAF-NPs) as bimodal contrast agents for cell labeling and tracking showed high contrast in both MRI and CT imaging. These nanoparticles accumulated in the liver and spleen after injection, leading to significant MRI contrast enhancement. Furthermore, the cell accumulation in the lungs after injection of SAF-NP-labeled cells was detectable using CT but not MRI. SAF-NPs are efficient agents for complementary MRI- and CT-based cell tracking, particularly useful in applications where visualization of particles and cells in all organs is needed.
Article
Engineering, Electrical & Electronic
Senne Fransen, Kherim Willems, Harold Philipsen, Devin Verreck, Willem Van Roy, Olivier Y. F. Henry, Antonio Arreghini, Geert Van den Bosch, Arnaud Furnemont, Maarten Rosmeulen
Summary: In this paper, a storage memory device based on the electro-deposition and electrodissolution of multilayered metal stacks in deep nanometer-sized wells is proposed, which enables bit densities of >1 Tbit/mm(2). It addresses the challenge of bit density scaling slowdown expected for 3-D NAND flash beyond 2030 and provides a proof-of-principle demonstration of the write/read mechanism on millimeter- and micrometer-sized electrodes, showing the device's potential for reaching very high bit densities. Simple analytical expressions based on finite element simulations are derived to evaluate the response time scaling for nanometer-sized electrodes in the envisioned device.
IEEE TRANSACTIONS ON ELECTRON DEVICES
(2022)
Article
Chemistry, Analytical
Pieter Berden, Rodrigo S. Wiederkehr, Liesbet Lagae, Jan Michiels, Tim Stakenborg, Maarten Fauvart, Willem Van Roy
Summary: We developed an analytical model that explains the relationship between amplification efficiency, template accessibility, and rain in dPCR assays. Through Monte Carlo simulations, we found that reduced amplification efficiency leads to broader threshold cycle distributions, while template inaccessibility results in an exponential tail rain pattern.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Sybren Santermans, Geert Hellings, Marc Heyns, Willem Van Roy, Koen Martens
Summary: Electrolyte-gated silicon FETs have the potential to detect single molecules, benefiting the development of high-throughput molecular sensing chips in genomics or proteomics. However, the sensitivity of these FETs to charges near the gate oxide is strongly reduced by electrolyte screening. We found that nano-scaling all FET channel dimensions is necessary to achieve a sufficiently large single-molecule SNR. In our study, a suspended nanowire FET architecture with specific dimensions showed the highest SNR of about 10 for DNA detection in an electrolyte solution.
Proceedings Paper
Thermodynamics
Grim Keulemans, Boshen Liang, Alexey Podkovskiy, Shruti Jambaldinni, Dominika Wysocka, Lei Zhang, Tim Stakenborg, David Cheyns, Paul Heremans, Veronique Rochus
Summary: In this study, fluid-structure interaction modeling was used to investigate the operation of an electrostatic actuated gas modulating microvalve. The simulation of valve position and gas flux during pull-in and snap-out events revealed voltage hysteresis and distinct flow patterns between the two events.
2021 22ND INTERNATIONAL CONFERENCE ON THERMAL, MECHANICAL AND MULTI-PHYSICS SIMULATION AND EXPERIMENTS IN MICROELECTRONICS AND MICROSYSTEMS (EUROSIME)
(2021)
Proceedings Paper
Engineering, Electrical & Electronic
Boshen Liang, Grim Keulemans, Dominika Wysocka, Alexey Podkovskiy, Lei Zhang, Veronique Rochus, Tim Stakenborg, Paul Heremans, David Cheyns
Summary: The integrated microfluidic system for next generation Lab-on-Chip systems is developed based on semiconductor-grade polymers and optimized excimer laser-aided transferring technique, enabling multi-step and highly controllable diagnostic point-of-care platforms with integrated electrical and optical detection schemes.
2021 5TH IEEE ELECTRON DEVICES TECHNOLOGY & MANUFACTURING CONFERENCE (EDTM)
(2021)
Article
Medical Laboratory Technology
Angela M. Jimenez Valencia, Lindsay A. Kryszak, Joshua Goheen, Willem Van Roy, Gabrielle Woronoff, Evelien Mathieu, Hildur Gudjonsdottir, Tim Stakenborg, William A. Clarke
Summary: Enzymatic assays are commonly used in clinical laboratories for diagnostic tests. A novel enzymatic method for measuring plasma creatinine using kinetic analysis with SOX as the limiting enzyme has been developed and shown to be rapid, sensitive, specific, and compatible with emerging technologies for portable diagnostic devices.
JOURNAL OF APPLIED LABORATORY MEDICINE
(2021)
Article
Engineering, Electrical & Electronic
Senne Fransen, Kherim Willems, Harold Philipsen, Devin Verreck, Willem Van Roy, Olivier Y. F. Henry, Antonio Arreghini, Geert Van den Bosch, Arnaud Furnemont, Maarten Rosmeulen
Summary: The proposed storage memory device achieves high bit densities through electro-deposition and electrodissolution in deep nanometer-sized wells, addressing the challenge of bit density scaling slowdown in future 3-D NAND flash. Experimental results on millimeter- and micrometer-sized electrodes demonstrate the device's potential for achieving very high bit densities.
IEEE TRANSACTIONS ON ELECTRON DEVICES
(2022)
Article
Biotechnology & Applied Microbiology
Yiran Qu, Innocent Bekard, Ben Hunt, Jamie Black, Louis Fabri, Sally L. Gras, Sandra. E. Kentish
Summary: This study compares the performance of a nanofiber device and a resin column for antibody capture. The nanofiber device has a larger housing volume and lower binding capacity, but comparable eluate purity to the resin column. It shows high stability, can be used for multiple cycles, and maintains consistent eluate quality when scaled up. The use of a single nanofiber device can significantly reduce costs compared to a resin column, especially when the number of batches is limited.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Brandon Moore, Christos Georgakis, Chris Antoniou, Sarwat Khattak
Summary: Fed-batch cell culture processes are commonly used in biomanufacturing due to their simplicity and applicability in cGMP environments. However, the challenge lies in the changing physiochemical conditions within the bioreactor as the cell density changes. Traditional response surface models (RSMs) are commonly used for optimization but are limited by their use of time-invariant factors. Dynamic RSM (DRSM) models can predict the time-dependent impact of process inputs, allowing for optimization of process operations that change over time.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lin Li, Yunfan Bai, Chuhua Qi, Yile Du, Xiaoxiao Ma, Yutong Li, Pingping Wu, Shuangli Chen, Sijing Zhang
Summary: A succinic anhydride-modified apple pomace (SAMAP) was synthesized to address environmental issues caused by the accumulation of apple pomace and effectively treat heavy metal ions. SAMAP exhibited high adsorption capacity for Cu(II) and Pb(II), suggesting its potential application in wastewater treatment.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Peter Satzer
Summary: Water for injection (WFI) production in the biopharmaceutical industry consumes excessive amounts of water and energy. Recycling buffers can potentially save up to 90% of resources, but achieving the full theoretical potential is impossible when a risk-aware design is used. Universal risk-based assessment is important for regulatory authorities to consider the implementation of such a strategy.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Gaoya Sun, Lingkai Jin, Guangxue Wang, Xiaoge Wang, Jin Huang
Summary: In this study, heterologous expression and homologous overexpression of ABC transporter proteins AatA and MdlB were found to improve butyric acid production in C. tyrobutyricum. The overexpression of these proteins upregulated the expression levels of key enzymes in the acetate synthesis pathway and promoted the synthesis and secretion of acetic acid. Additionally, the increase in ATPase activity facilitated sugar utilization, induced extracellular secretion of acetate, and shortened fermentation periods.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Taiki Okamura, Rina Aritomi, Takuya Matsumoto, Ryosuke Yamada, Hidehiko Hirakawa, Hiroyasu Ogino
Summary: In this study, proline was introduced to improve the stability of putidaredoxin reductase (PdR) in the Pseudomonas putida cytochrome P450 system. It was found that PdR_T221P had a longer half-life at high temperatures compared to wild-type PdR, but a shorter half-life in the presence of methanol. Molecular dynamics simulations supported these findings.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yuying Hu, Xiaofan Wang, Shihao Zhang, Zimu Liu, Tengfang Hu, Xin Wang, Xiaoming Peng, Hongling Dai, Jing Wu, Fengping Hu
Summary: This study investigates the effect of iron-carbon micro-electrolysis (ICME) materials on high-solid anaerobic digestion (HSAD). The results show that ICME materials promote methane production in HSAD by increasing the attachment area of microorganisms and facilitating symbiotic metabolism of certain bacterial species. This study provides new insights into microbial mechanisms and enhances our understanding of ICME material enhancement in HSAD.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jibao Liu, Yufeng Xu, Yuansong Wei
Summary: This study investigated the role of sludge rheology in anaerobic digestion (AD) and found that rheological properties increased with the increase of solid content, resulting in a negative effect on methane production. An extended ADM1 model revealed that enhanced sludge rheological properties increased mass diffusion resistance and reduced uptake rate of acetate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyan Liu, Zongze Chen, Dewen Kong, Xinying Zhang, Chuanhua Wang, Yongqi Wang
Summary: This study explored the role of intracellular and extracellular enzymes of Acinetobacter baumannii and Talaromyces sp. in the degradation of crude oil. The extracellular enzymes of Talaromyces sp. were more effective in degrading n-alkanes, while those of Acinetobacter baumannii had a better effect on aromatic hydrocarbons. The degradation enzyme systems of both bacteria and fungi complemented each other, improving the overall degradation ability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Jing Dong, Lingli Xu, Yuxiang Liu, Li Ren, Ke Yuan
Summary: The utilization of biochar-immobilized microorganisms is an effective method for eliminating phenol from water. The high susceptibility of bacteria to environmental factors is a challenge for practical implementation. In this study, biochar was used to reduce microbial susceptibility and enhance phenol removal. The addition of biochar altered the dominant species of phenol-degrading bacteria and response surface analysis indicated the significant influence of biochar pyrolysis temperature and experimental temperature on phenol removal rate.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Zibin Pan, Mengying Liu, Zuliang Chen
Summary: This study successfully removed metalloids and heavy metals from acid mine drainage (AMD) using bio-synthesized Fe/Cu nanoparticles (Fe/Cu NPs). The Fe/Cu NPs showed high removal capacities and the presence of organic substances contributed to their stability.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Guangbing Liu, Han Zhang, Jincan Huang, Lu Zhang, Teng Zhang, Xuemin Yu, Weijing Liu, Chunkai Huang
Summary: This study investigated the effect of Fenton pre-treatment on the treatment efficiency of printing and dyeing wastewater (PDW) using two anaerobic/aerobic-membrane bioreactors (A/O-MBRs). The results showed that Fenton pre-treatment significantly improved the removal efficiency of COD and AOX in PDW, and reduced membrane fouling. The Shannon indices and metagenomics analysis indicated that the microbial diversity in anaerobic flocs was higher than that in aerobic flocs, and EC3.1.1.45 and pcaI were identified as key functional genes.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Lidia Borgosz, Duygu Dikicioglu
Summary: The Industrial Internet of Things (IIoT) is a system that connects devices and provides real-time insight into industrial processes. However, the complexity and regulatory requirements of the biomanufacturing sector make it challenging to implement IIoT. There is a need for universal solutions to overcome this challenge and advance the field of biomanufacturing.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yajie Li, Weikang Kong, Yuyao Zhang, Huarui Zhou, Hongbo Liu, Salma Tabassum
Summary: In this study, the iron-carbon multi-micro electric field coupling anaerobic co-digestion technique was used to treat coal gasification wastewater (CGW). The experimental results showed that under optimal operating conditions, this technique can significantly reduce the toxicity of the wastewater and achieve high removal efficiencies. Additionally, the analysis of microbial communities revealed that the coupling system promotes direct interspecies electron transfer.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Aparecido Nivaldo Modenes, Debora Gozzi Fernandes, Daniela Estelita Goes Trigueros, Matheus Guilherme Amador, Fernando Rodolfo Espinoza-Quinones, Taysa de Souza Braniz, Adilson Ricken Schuelter, Glacy Jaqueline da Silva, Lucimar Pereira Bonett
Summary: This study aimed to systematically remove organic pollutants from raw dairy wastewater with high concentrations of COD, TOC, and TN using Poterioochromonas malhamensis algae strains. The results showed that the biomass yield rate using FP-PBRs was 10% higher than tubular PBRs, and the organic pollution in wastewater was significantly reduced with a decrease of about 98% in COD, 95% in TN, and 92% in TOC.
BIOCHEMICAL ENGINEERING JOURNAL
(2024)