Article
Multidisciplinary Sciences
Yusuke Oizumi, Takuto Kaji, Sanki Tashiro, Yumiko Takeshita, Yuko Date, Junko Kanoh
Summary: The complete sequences of subtelomeric homologous (SH) regions of the fission yeast Schizosaccharomyces pombe were reported, revealing highly polymorphic and hot spots for genome variation features. These subtelomeric regions may contribute to genome diversity and potentially lead to various diseases.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Barbara Sivakova, Jan Jurcik, Veronika Lukacova, Tomas Selicky, Ingrid Cipakova, Peter Barath, Lubos Cipak
Summary: The LFQ phosphoproteomics workflow presented in this study, based on sequential Fe-IMAC phosphopeptide enrichment, strong anion exchange (SAX), and porous graphitic carbon (PGC) fractionation strategies, enabled the identification of 8353 phosphosites in the fission yeast Schizosaccharomyces pombe, with 1274 sites being newly discovered. The results demonstrate that the combination of PGC and SAX fractionation strategies significantly enhances the robustness and specificity of LFQ phosphoproteomics studies, providing valuable insights into the complexity of protein kinase functions in S. pombe.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jie Zhao, Sijie Liu, Clemens Alexander Wolf, Gerhard Wolber, Maria Kristina Parr, Matthias Bureik
Summary: Alprazolam, a commonly used drug for managing anxiety disorders, is metabolized by CYP3A enzymes. This study compared the metabolism of alprazolam by all four human CYP3A enzymes and found that CYP3A4 and CYP3A5 have the highest production rates of 4-hydroxyalprazolam, while CYP3A5 alone is the major producer of alpha-hydroxyalprazolam. Computational simulations explained the difference in preferred oxidation sites between CYP3A5 and CYP3A43. Unexpectedly, three CYP3A43 mutants produced a different metabolite, 5-N-O alprazolam. The mutants also showed higher production rates of 4-hydroxyalprazolam compared to the wild-type, while the wild-type did not produce 5-N-O alprazolam.
Review
Plant Sciences
Thibault Kosuth, Alexandra Leskova, Loren Castaings, Catherine Curie
Summary: Manganese (Mn) is crucial for plant growth and development, and recent studies have shed light on the processes controlling its homeostasis in the cell through the identification of new Mn transporters. These transporters, particularly those at the Golgi membranes, play a central role in regulating the synthesis of cell walls and the various Mn-dependent pathways. These findings have highlighted the importance of Golgi-resident transporters in maintaining cellular Mn levels.
Article
Biochemistry & Molecular Biology
Kim Kiat Lim, Hwei Yee Teo, Yuan Yee Tan, Yi Bing Zeng, Ulysses Tsz Fung Lam, Mahesh Choolani, Ee Sin Chen
Summary: MTHFR is a key enzyme in the folate metabolic pathway, linked to various human conditions. The fission yeast gene met11(+) shows similar function to MTHFR, affecting chromosome stability and meiotic fidelity. Cooperation among Met11, Rec8, and Sgo1 is crucial for proper genetic inheritance during mitosis and meiosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biotechnology & Applied Microbiology
R. Sahaya Glingston, Jyoti Yadav, Jitika Rajpoot, Neha Joshi, Shirisha Nagotu
Summary: Yeast has been proven to be a valuable model system for studying viral infection and expression of viral proteins, advancing our understanding of important viruses. Through high-throughput analysis, genetic manipulation, and its compact genome, yeast provides insights into various aspects related to virus replication.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Bandita Mohapatra, Nimisha Sharma
Summary: Biosynthesized Ag-ZnO nanohybrids were characterized using various techniques and were found to have significant antifungal activity against Schizosaccharomyces pombe. It was discovered that the antifungal action of these nanohybrids is mainly due to the generation of reactive oxygen species (ROS), which leads to oxidative stress, lipid peroxidation, and cell damage. These findings provide insights into the mechanism of action and potential applications of Ag-ZnO nanohybrids for antifungal purposes.
MATERIALS TODAY COMMUNICATIONS
(2023)
Article
Oncology
Han-Chao Feng, Christos Andreadis, Ji-Long Liu
Summary: The formation of cytoophidia in fission yeast is influenced by the histone chaperone Slm9, as knockout of the slm9 gene leads to fragmentation and changes in morphology of the cytoophidia. This study reveals a potential link between a chromosomal regulatory factor and cytoophidium biogenesis.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Cell Biology
Xiuping Sun, Divyanshu Mahajan, Bing Chen, Zhiwei Song, Lei Lu
Summary: The mechanism of Golgi localization of Golgi glycosyltransferases has been found to be primarily through retention rather than retrieval. The study also revealed the contributions of different regions of ST6GAL1 to Golgi retention, showing that the N-terminal cytosolic tail and transmembrane domain can act as Golgi export signals.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Ying-Ying Wang, Jianxiu Zhang, Xiao-Man Liu, Yulu Li, Jianhua Sui, Meng-Qiu Dong, Keqiong Ye, Li-Lin Du
Summary: This study uncovers the mechanism of how a receptor called Nbr1 in fission yeast recognizes specific protein cargos, revealing the mechanism of autophagy cargo recognition. The research shows that Nbr1-ZZ1 can not only recognize the N-termini of cargos via a conserved acidic pocket, but also engage other parts of the cargos in a cargo-specific manner.
Article
Multidisciplinary Sciences
Akihisa Matsuyama, Atsushi Hashimoto, Shinichi Nishimura, Minoru Yoshida
Summary: Considering the frequent use of leu1 and ura4 genes in fission yeast, we developed new host-vector systems by editing lys1 and arg3 genes with the CRISPR/Cas9 system. We obtained critical mutations in the ORF region and developed complementary vectors for lys1 and arg3 mutants. By combining these vectors with the integration vector pDUAL, we successfully visualized the localization of three proteins fused with different fluorescent proteins in a single cell. Thus, these vectors enable combinatorial expression of heterologous genes, addressing the increasingly diverse experimental challenges.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Karen Baker, Michael A. Geeves, Daniel P. Mulvihill
Summary: This study investigates the impact of amino-terminal acetylation on the structure and function of fission yeast calmodulin. The findings show that NatA-dependent acetylation stabilizes the structure and affects the ability of calmodulin to bind to myosin at endocytic foci. Furthermore, the study reveals that this conserved modification also influences the calcium-binding capacity of both yeast and human calmodulins.
Article
Genetics & Heredity
Alia Ebrahim, Manal A. Alfwuaires, Mohammad H. Abukhalil, Fawaz Alasmari, Fawad Ahmad, Rui Yao, Ying Luo, Ying Huang
Summary: This study used bioinformatics analysis to investigate the role of three genes, spGrx4, spFep1, and spPhp4, in maintaining iron homeostasis in the fission yeast Schizosaccharomyces pombe. Multiple sequence alignment and protein-protein interaction analysis provided insights into their similarities with sequence homologs in other species and their cooperative roles in iron homeostasis.
FRONTIERS IN GENETICS
(2022)
Article
Microbiology
Yuhei O. Tahara, Makoto Miyata, Taro Nakamura
Summary: This study visualized S. pombe spores using QFDE-EM technology, revealing novel structural features including the interwoven fibrillar Isp3 layer and deep invaginations distinct from vegetative cell membranes. Observations of spore germination showed a transition in cell surface structure from smooth to dendritic filamentous, characteristic of vegetative cells.
Article
Oncology
Christos Andreadis, Tianhao Li, Ji-Long Liu
Summary: In this study, it was found that ubiquitination plays an important role in regulating the formation of cytoophidia. The proteins associated with CTPS and the ubiquitination regulators that significantly affect CTPS filamentation were identified. Additionally, a group of deubiquitinating enzymes were found to be important for regulating the filamentous morphology of cytoophidia.
EXPERIMENTAL CELL RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Thi Sam Nguyen, Ryo Misaki, Takao Ohashi, Kazuhito Fujiyama
Article
Multidisciplinary Sciences
Hiroyuki Kajiura, Ryousuke Miyauchi, Akemi Kakudo, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama
Summary: N-glycosylation is a significant post-translational modification in eukaryotic cells, with limited information on insect N-glycosylation. The N-acetylgalactosaminyltransferase in Bombyx mori is involved in complex N-glycan biosynthesis, localizing in the Golgi and being expressed in various organs and developmental stages. Despite a multifunctional glycosyltransferase, the N-glycosylation in B. mori is strictly regulated by endogenous machineries.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Chih-Chan Wu, Takao Ohashi, Hiroyuki Kajiura, Yu Sato, Ryo Misaki, Kohsuke Honda, Savitree Limtong, Kazuhito Fujiyama
Summary: The overexpression of RtFAD2 in oleaginous yeast Rhodotorula toruloides significantly increased the production of linoleic acid (LA), indicating a potential application in the production of LA or polyunsaturated fatty acids. This finding may assist in the rational design of metabolic engineering related to LA production in R. toruloides.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)
Article
Plant Sciences
Ratna Sariyatun, Hiroyuki Kajiura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama
Summary: This study demonstrated the production of an alternative acid-alpha glucosidase (GAA) for enzyme replacement therapy of Pompe disease in a glycoengineered Arabidopsis alg3 cell culture. Supplementing the culture medium with NaCl to a final concentration of 50 mM increased GAA production by 3.8-fold, without affecting N-glycosylation.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Takamasa Fukunaga, Yuki Sakurai, Takao Ohashi, Yujiro Higuchi, Hiromi Maekawa, Kaoru Takegawa
Summary: Through a genome-wide screen of a deletion mutant strain in fission yeast, two genes (pwp1(+), SPBC1E8.05) encoding GPI-anchored proteins were identified to improve the growth rate of cells lacking the outer chain, thereby enhancing productivity of heterologous proteins. Mutational analysis revealed the importance of the GPI-anchored region of Pwp1p in attenuating the growth defect and supporting beta-glucan on the cell-surface of S. pombe non-enzymatically.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ryo Misaki, Masashi Iwasaki, Hiroki Takechi, Noriko Yamano-Adachi, Takao Ohashi, Hiroyuki Kajiura, Kazuhito Fujiyama
Summary: Although antibodies are promising biopharmaceuticals, the high costs of purification and cell cultivation have posed challenges. This study successfully produced afucosylated antibodies by developing a Chinese hamster ovary cell line with no fucosylated glycans. The researchers also discovered that fucosylation occurs due to contamination from serum components.
Article
Biotechnology & Applied Microbiology
Takao Ohashi, Nabilah Sari, Ryo Misaki, Kazuhito Fujiyama
Summary: In this study, the researchers cloned the genes encoding Golgi-localized PG proteins in Arabidopsis, and characterized the enzymatic properties of AtPGF10.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Cell Biology
Hiromi Maekawa, Shen Jiangyan, Kaoru Takegawa, Gislene Pereira
Summary: The mitotic exit network (MEN) is important for terminating mitosis in yeasts, but its molecular mechanisms differ between different species.
Article
Biotechnology & Applied Microbiology
Takamasa Fukunaga, Takao Ohashi, Yutaka Tanaka, Tomoki Yoshimatsu, Yujiro Higuchi, Hiromi Maekawa, Kaoru Takegawa
Summary: Schizosaccharomyces japonicus requires Gal-containing oligosaccharides for cell wall integrity during filamentous growth. The cell-surface of S. japonicus primarily consists of tri-saccharides (Gal-Man-Man), unlike other species. Disruption of the UDP-Gal transporter gene results in a complete lack of Gal on the cell surface and blocks hyphal development.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Edjohn Aaron Macauyag, Hiroyuki Kajiura, Takao Ohashi, Ryo Misaki, Kazuhito Fujiyama
Summary: This study demonstrates a high-level transient production system of functional PRbFGF in N. benthamiana leaves and an efficient tag-less purification technique of leaf crude extracts. The optimized Agrobacterium-mediated transient expression system achieved a 3-fold increase in bFGF production, and the introduction of a mutant protease-resistant version further doubled the yield. The purified PRbFGF showed comparable cell proliferation activity to E. coli-produced bFGF.
PLANT BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Akira Hosomi, Chinatsu Okachi, Yudai Fujiwara
Summary: Soluble proteins with a signal peptide are translocated into the endoplasmic reticulum (ER), while some proteins without a signal peptide, like SOD1, can still enter the ER. SOD1 is a causative gene of ALS, and its secretion has been implicated in the pathogenesis of ALS. This study reveals that SOD1 is translocated into the ER lumen through the translocon Sec61 and subsequently secreted extracellularly, suggesting the potential of targeting SOD1 secretion as a therapeutic approach for ALS.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Takamasa Fukunaga, Naotaka Tanaka, Toshio Furumoto, Shinichi Nakakita, Takao Ohashi, Yujiro Higuchi, Hiromi Maekawa, Kaoru Takegawa
Summary: Eight galactosyltransferases in S. pombe have been shown to have different substrate specificities and are involved in galactosylation of N- and O-glycans.
Article
Biotechnology & Applied Microbiology
Emiko Matsunaga, Yutaka Tanaka, Saki Toyota, Hisae Yamada, Takuji Oka, Yujiro Higuchi, Kaoru Takegawa
Summary: In this study, two Galf-specific Galf-ase genes, gfgA and gfgB, were identified in Aspergillus nidulans. The encoded proteins exhibited Galf-specific Galf-ase activity without Araf-ase activity. Additionally, Galf-ases in Aspergillus species may cooperatively degrade Galf-containing oligosaccharides depending on environmental conditions.
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2021)