Article
Medicine, General & Internal
Xingwang Jia, Pengjun Zhang, Yaping Tian, Junli Wang, Huadong Zeng, Jun Wang, Jiao Liu, Zeyan Chen, Lijun Zhang, Haihong He, Kunlun He, Yajie Liu
Summary: This study aimed to investigate the clinical significance of IgM and IgG testing in the diagnosis of highly suspected COVID-19 patients, with positive detection rates being higher when both IgM and IgG testing were combined. The method provides a rapid, simple, and accurate way to diagnose COVID-19, especially in patients who initially test negative for nucleic acid.
FRONTIERS IN MEDICINE
(2021)
Article
Immunology
Jennifer Serwanga, Violet Ankunda, Jackson Sembera, Laban Kato, Gerald Kevin Oluka, Claire Baine, Geoffrey Odoch, John Kayiwa, Betty Oliver Auma, Mark Jjuuko, Christopher Nsereko, Matthew Cotten, Nathan Onyachi, Moses Muwanga, Tom Lutalo, Julie Fox, Monica Musenero, Pontiano Kaleebu
Summary: This study aimed to investigate the development of antibody responses in mild and asymptomatic COVID-19 cases in Africa and their interactions with SARS-CoV-2. The results showed that asymptomatic patients exhibited a faster and more robust antibody response against SARS-CoV-2 spike protein compared to mild symptomatic patients. The spike IgG antibodies were more durable and lasted for 28 months.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Ojas Kaduskar, Yogesh K. Gurav, Ketki Deshpande, Gururaj Rao Desphande, Pragya Yadav, Aparana Rakhe, Bipin N. Tilekar, Prasad Gomade, Asha Salunke, Chetan Patil, Varsha Dange, Pavan Salve, Sudhir Patsute, Priya Abraham, Gajanan N. Sapkal
Summary: This study investigated the dynamics and persistence of IgG and IgM antibodies in COVID-19 patients using chemiluminescent assay. The results showed that both symptomatic and asymptomatic patients started producing antibodies from day five of onset of disease. IgG antibodies persisted for a longer period compared to IgM antibodies. However, relying solely on binding antibody values may not be a perfect guide for selecting plasma therapy donors.
INDIAN JOURNAL OF MEDICAL RESEARCH
(2022)
Article
Medicine, General & Internal
Jie Lin, Wei Dai, Weiwei Li, Li Xiao, Tao Luo, Yanju Guo, Yang Yang, Ying Han, Peiran Zhu, Qiuyue Wu, Bangshun He, Jian Wu, Xinyi Xia
Summary: This study tested seven antibody detection kits from four manufacturers for detection of SARS-CoV-2 IgG, IgM, and total antibody under different heat-inactivation conditions. The results indicated that heat-inactivation could potentially cause false-positive or false-negative results for COVID-19 antibody detection using the indirect immunity method, with increased IgG antibody values and decreased IgM antibody values.
FRONTIERS IN MEDICINE
(2021)
Article
Clinical Neurology
Renata Barbosa Paolilo, Carolina de Medeiros Rimkus, Jose Albino da Paz, Samira Luisa Apostolos-Pereira, Dagoberto Callegaro, Douglas Kazutoshi Sato
Summary: This study evaluated the MRI findings of pediatric-onset AQP4-IgG positive NMOSD and found that most lesions either reduced, remained stable, or developed atrophy/cavitation. Some patients had asymptomatic brain lesions or enlargement of existing lesions, and these lesions also occurred during acute attacks in other locations. One patient had an asymptomatic spinal cord lesion, and no asymptomatic lesions were observed in optic nerve MRI. Acute phase exams showed longitudinally extensive transverse myelitis, cervical myelitis, and lumbar myelitis.
MULTIPLE SCLEROSIS AND RELATED DISORDERS
(2022)
Article
Biotechnology & Applied Microbiology
G. Vinaya Chandu Vidya Sagar, P. V. Janardhan Reddy, Prashanth Suravajhala, Renuka Suravajhala, V. Uday Kiran, P. B. Kavi Kishor, T. C. Venkateswarulu, Rathnagiri Polavarapu
Summary: In this study, the effectiveness of an in-house developed rapid antibody assay for COVID-19 was evaluated and found to be satisfactory in determining virus infection and evaluating immune response. These findings support the widespread utilization of serological antibody testing in COVID-19 diagnosis.
Article
Biochemical Research Methods
Thisara Jayawickrama Withanage, Rami Krieger, Ellen Wachtel, Guy Patchornik
Summary: IgG antibodies provide longer term immunity against infections compared to IgM antibodies. It is necessary to separate IgG from IgM for diagnostic purposes. A purification platform using conjugated Brij-O20 or Tween-20/DDM/Tyr micelles has been developed to efficiently recover IgG with high purity, while IgM recovery yields are lower and contain small amounts of co-extracted IgG. Chromatographic separation with Protein A or Protein G resins also achieve similar yields but lower purity.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Infectious Diseases
Armel Landry Batchi-Bouyou, Line Lobaloba Ingoba, Matthieu Ndounga, Jeannhey Christevy Vouvoungui, Claujens Chastel Mfoutou Mapanguy, Kamal Rauchelvy Boumpoutou, Francine Ntoumi
Summary: The Republic of the Congo conducted a seroprevalence study on healthy individuals to evaluate the incidence of SARS-CoV-2, finding a certain proportion testing positive for the virus. Among RT-PCR negative subjects, the seroprevalence of IgG and IgM antibodies increased over time, with higher rates in women compared to men.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Virology
Keren Kang, Lingfang Huang, Chaolin Ouyang, Jiaming Du, Bin Yang, Yaqin Chi, Shuyu He, Le Ying, Guoling Chen, Jihua Wang
Summary: A Rapid Antibody Test Kit was developed for the sensitive, accurate, and automated detection of IgM and IgG antibodies against SARS-CoV-2 in human samples, showing good performance in sensitivity, specificity, precision, and stability. The combined use of IgM and IgG as an alternative detection method for COVID-19 patients achieved high sensitivity and specificity, making it of great public health significance for the prevention and control of the pandemic.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Chemistry, Analytical
Qiaoge Bayin, Lei Huang, Chunhui Ren, Yusheng Fu, Xing Ma, Jinhong Guo
Summary: The study introduces a novel lateral flow immunoassay method based on superparamagnetic nanoparticles and giant magnetoresistance sensing system for simultaneous quantitative detection of anti-SARS-CoV-2 immunoglobulin M and G. This method offers advantages in cost-effectiveness, time efficiency, and ease of operation.
Article
Virology
Zu-Li Zhang, Yu-Lei Hou, De-Tao Li, Feng-Zeng Li
Summary: The detection of anti-SARS-CoV-2 IgG and IgM showed high diagnostic efficiency in assisting the diagnosis of SARS-CoV-2 infection, with gold immunochromatography assay potentially being the preferred method.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Immunology
Hannah A. D. King, Vincent Dussupt, Letzibeth Mendez-Rivera, Bonnie M. M. Slike, Ursula Tran, Nathan D. D. Jackson, Erica Barkei, Michelle Zemil, Emily Tourtellott-Fogt, Caitlin H. H. Kuklis, Sandrine Soman, Aslaa Ahmed, Maciel Porto, Christopher Kitajewski, Brittany Spence, Dalia Benetiene, Lindsay Wieczorek, Swagata Kar, Gregory Gromowski, Victoria R. R. Polonis, Shelly J. J. Krebs, Kayvon Modjarrad, Diane L. L. Bolton
Summary: This study investigates the protective effects of convalescent immunoglobulins against SARS-CoV-2 in a Syrian golden hamster model. It shows that IgM has higher neutralization potency than IgG, but fails to protect hamsters from disease. IgG infusion, on the other hand, effectively prevents disease in a dose-dependent manner, with detectable neutralizing titers correlating with protection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Virology
Wei Zhou, Xiaomao Xu, Zhigang Chang, He Wang, Xuefeng Zhong, Xunliang Tong, Taotao Liu, Yanming Li
Summary: This study observed significant decline in IgM and IgG antibodies in COVID-19 patients between weeks 4 to 10 after symptom onset. Notably, 77.5% of patients experienced a rapid reduction by half in IgG levels within the second month. Furthermore, in the recovery stage, IgG levels decreased significantly by a median of 50%.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Immunology
Baolin Liao, Zhao Chen, Peiyan Zheng, Linghua Li, Jianfen Zhuo, Fang Li, Suxiang Li, Dingbin Chen, Chunyan Wen, Weiping Cai, Shanhui Wu, Yanhong Tang, Linwei Duan, Peilan Wei, Fangli Chen, Jinwei Yuan, Jinghong Yang, Jiaxin Feng, Jingxian Zhao, Jincun Zhao, Baoqing Sun, Airu Zhu, Yimin Li, Xiaoping Tang
Summary: Characterizing the serologic features of asymptomatic SARS-CoV-2 infection is crucial for diagnostics and control. This study found that asymptomatic infections exhibit weaker overall antibody responses, primarily inducing IgG antibody responses. Anti-S2-IgG shows better sensitivity in identifying asymptomatic infections early after infection compared to anti-RBD-IgG.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Shun Kaneko, Yoko Nukui, Takeshi Arashiro, Yoshibumi Aiso, Maya Sugii, Yoshiro Hadano, Kaoru Nagata, Reiko Taki, Ken Ueda, Satoko Hanada, Shinichiro Suzaki, Naoshige Harada, Yoshimi Yamaguchi, Hiroyuki Nakanishi, Masayuki Kurosaki, Masayuki Nagasawa, Namiki Izumi
Summary: This study evaluated the clinical performance of the colloidal gold immunochromatography assay for SARS-CoV-2 IgM/IgG antibodies in COVID-19 patients, showing a high sensitivity and specificity for the diagnosis of the disease.
JOURNAL OF MEDICAL VIROLOGY
(2021)