Journal
COMMUNICATIONS BIOLOGY
Volume 3, Issue 1, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s42003-020-1011-4
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Funding
- MEXT/JSPS KAKENHI [JP19K16088, JP17K07361, JP19KK0071, JP20K06579, JP17K07816, JP18H05229, JP18H05534, JP18H03681]
- Future Development Funding Program ofKyoto University Research Coordination Alliance
- Research Fund for Young Scientists in Kyoto University
- Fund for Project Research in Institute for Integrated Radiation and Nuclear Science, Kyoto University (KURNS)
- project for Construction of the basis for the advanced materials science and analytical study by the innovative use of quantum beam and nuclear sciences in KURNS
- IMS [205]
- Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED [JP20am0101076]
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Currently, a sample for small-angle scattering (SAS) is usually highly purified and looks monodispersed: The Guinier plot of its SAS intensity shows a fine straight line. However, it could include the slight aggregates which make the experimental SAS profile different from the monodispersed one. A concerted method with analytical-ultracentrifugation (AUC) and SAS, named as AUC-SAS, offers the precise scattering intensity of a concerned biomacromolecule in solution even with aggregates as well that of a complex under an association-dissociation equilibrium. AUC-SAS overcomes an aggregation problem which has been an obstacle for SAS analysis and, furthermore, has a potential to lead to a structural analysis for a general multi-component system. Ken Morishima et al. integrate small-angle scattering (SAS) with analytical-ultracentrifugation (AUC) to analyze the scattering intensity of biomacromolecules in solution. Their new approach allows to correct for the aggregation effect and can be applied to multi-component systems.
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