Article
Cell Biology
Maria Giovanna Riparbelli, Veronica Persico, Giuliano Callaini
Summary: During early oogenesis in Drosophila, there are specific spatial interactions between somatic and germ cells, indicating inherent asymmetry between the anterior and posterior regions of the egg chambers. The oocyte is located at the posterior end, giving the egg chamber distinct asymmetry.
Article
Agriculture, Dairy & Animal Science
Atsuko Kageyama, Ayumi Suyama, Ruka Kinoshita, Junya Ito, Naomi Kashiwazaki
Summary: This study investigated the amounts and localization of zinc ions during maturation, fertilization, activation, and embryonic development in mouse oocytes. The results showed a dramatic decrease of zinc ions during fertilization and activation, but a rapid increase in blastocysts.
ANIMAL SCIENCE JOURNAL
(2022)
Article
Cell Biology
Lili Guo, Yongda Zhao, Yanjun Huan
Summary: The study showed that chlorpyrifos had toxic effects on porcine oocytes, including delaying meiotic progression, decreasing polar body extrusion, disrupting spindle assembly and causing DNA damage, as well as impairing cytoplasmic maturation. Pterostilbene was able to effectively alleviate the damage caused by chlorpyrifos and improve oocyte maturation and embryonic development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Environmental Sciences
Yong-Sheng Wang, Sheng-Ji Yang, Muhammad Jamil Ahmad, Zhi-Ming Ding, Ze-Qun Duan, Yang-Wu Chen, Ming Liu, Ai-Xin Liang, Guo-Hua Hua, Li-Jun Huo
Summary: This study investigated the adverse effects of Zinc Pyrithione (ZPT) on mouse oocyte maturation and quality. The results showed that ZPT compromised oocyte maturation by disrupting meiotic cell cycle and causing cytoskeletal defects. In addition, ZPT altered epigenetic modifications and resulted in zinc accumulation, which further impaired the quality of oocytes.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Cell Biology
Long Jin, Hai-Ying Zhu, Xiang-Jin Kang, Li-Ping Lin, Pu-Yao Zhang, Tao Tan, Yang Yu, Yong Fan
Summary: Oxybenzone, a common UV filter in sunscreens and cosmetics, has been found to have negative effects on oocyte maturation, but melatonin shows protective effects against these detrimental effects.
Article
Reproductive Biology
Z. Trebichalska, D. Kyjovska, S. Kloudova, P. Otevrel, A. Hampl, Z. Holubcova
Summary: Ultrastructural analysis of maturing human oocytes reveals complex changes in cytoplasmic architecture associated with the acquisition of egg's developmental competence. The study proposes a morphological map of cytoplasmic maturation, improving our understanding of human egg morphology, quality, and intracellular factors. This analysis provides essential insight into the enigmatic process by which human egg progenitors prepare for fertilization.
BIOLOGY OF REPRODUCTION
(2021)
Article
Cell Biology
Virginia Savy, Paula Stein, Min Shi, Carmen J. Williams
Summary: The study found that while there were subtle differences in calcium signaling in eggs following superovulation during parthenogenetic activation, there was no impact on the physiological calcium signaling at fertilization. This suggests that superovulation does not disrupt normal calcium signaling during fertilization, supporting its use for both clinical and experimental purposes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Reproductive Biology
Rebecca L. Krisher
Summary: This review examines the current use of in vitro oocyte maturation in clinical settings, including its different definitions in practice. It discusses the role of in vitro oocyte maturation in patient care and the challenges for implementation, as well as exploring its efficiency and safety. The review also discusses the role of in vitro oocyte maturation in oncofertility and provides an outlook for its future in clinical settings.
BIOLOGY OF REPRODUCTION
(2022)
Review
Cell Biology
Sherif Mostafa, Nancy Nader, Khaled Machaca
Summary: Cell lipids play important roles in signaling, especially during the maturation of reproductive cells. This review summarizes the current understanding of lipid signaling and proposes future research directions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Zhi-Yong Yang, Min Ye, Ya-Xin Xing, Qi-Gui Xie, Jian-Hong Zhou, Xin-Rui Qi, Kehkooi Kee, Ri-Cheng Chian
Summary: By performing single-cell analysis, this study identified the differentially expressed genes (DEGs) and pathways related to mitochondria during human oocyte maturation. The results showed that oxidative phosphorylation, pyruvate metabolism, peroxisome, and amino acid metabolism pathways played important roles in oocyte maturation. Additionally, it was observed that mitochondria were regulated by nuclear genes to meet the developmental needs, and the current in vitro maturation culture medium should be optimized based on these findings.
Article
Reproductive Biology
Yue Wang, Chun-Hua Xing, Shun Chen, Shao-Chen Sun
Summary: The study showed that ZEN exposure disrupted organelle function in porcine oocyte meiotic maturation, affecting mitochondria, endoplasmic reticulum, Golgi apparatus, and lysosome.
Article
Fisheries
Yuya Hasegawa, Shigeho Ijiri, Ryohei Surugaya, Ryuichi Sakai, Shinji Adachi
Summary: This study revealed that the sturgeon hsd17b12L gene encodes 20 beta-H5D, playing a key role in regulating DHP production. Transfection experiments and LC/MS analysis confirmed this function. The expression level of hsd17b12L gene was upregulated during sturgeon oocyte maturation, and stimulation with SPE increased DHP concentration.
Article
Biochemistry & Molecular Biology
Anna H. York-Andersen, Benjamin W. Wood, Elise L. Wilby, Alexander S. Berry, Timothy T. Weil
Summary: Egg activation is a complex process involving resumption of cell cycle, mRNA translation, and vitelline membrane cross-linking. While the mechanism of egg activation in insects is not well understood, in Drosophila, it requires volume increase and calcium entry.
Article
Obstetrics & Gynecology
Sylvie Lierman, Annelies Tolpe, Ilse De Croo, Stefanie De Gheselle, Justine Defreyne, Machteld Baetens, Annelies Dheedene, Roos Colman, Bjorn Menten, Guy T'Sjoen, Petra De Sutter, Kelly Tilleman
Summary: In this study, the feasibility of in vitro maturation of ovarian tissue oocytes for fertility preservation in transgender men on testosterone treatment was investigated. The results showed low developmental capacity of in vitro matured oocytes in these individuals under testosterone treatment, with abnormal development patterns observed. Additionally, the study found a negative association between the vitrification rate and progesterone levels, and a positive association with antimullerian hormone levels.
FERTILITY AND STERILITY
(2021)
Article
Reproductive Biology
Takuya Sato, Mao Hamazaki, Yuki Inoue, Sogo Aoki, Yuichi Koshiishi, Koumei Shirasuna, Hisataka Iwata
Summary: This study investigated the effects of low ethanol exposure on bovine oocytes. The results showed that even concentrations as low as 0.1% ethanol during in vitro maturation significantly affected oocyte metabolism and histone configuration of embryos. Ethanol exposure increased mitochondrial DNA copy number and protein levels, blastulation rate, ATP content and membrane potential, while decreasing lipid content and glucose consumption.
Article
Cell Biology
Mingyue Wang, Qinan Hu, Tianhang Lv, Yuhang Wang, Qing Lan, Rong Xiang, Zhencheng Tu, Yanrong Wei, Kai Han, Chang Shi, Junfu Guo, Chao Liu, Tao Yang, Wensi Du, Yanru An, Mengnan Cheng, Jiangshan Xu, Haorong Lu, Wangsheng Li, Shaofang Zhang, Ao Chen, Wei Chen, Yuxiang Li, Xiaoshan Wang, Xun Xu, Yuhui Hu, Longqi Liu
Summary: This study utilized Stereo-seq technology to analyze the spatiotemporal transcriptome of developing Drosophila with high resolution and sensitivity. The results revealed the spatial transcriptomic characteristics of Drosophila embryos and larvae, and provided key information for the analysis of transcription factor regulons.
DEVELOPMENTAL CELL
(2022)
Article
Genetics & Heredity
Aaron C. Sue, Sarah M. Wignall, Teresa K. Woodruff, Thomas O'Halloran
Summary: This study investigates the role of zinc transporters in reproductive systems using the nematode Caenorhabditis elegans as a model. Phenotypic analysis of mutants reveals that two zinc transporters, ZIPT-2.4 and ZIPT-15, play an important role in reproductive output and gonad development. These findings suggest a complex zinc trafficking network that is crucial for reproductive success.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2022)
Article
Multidisciplinary Sciences
Erin L. McCullough, Emma Whittington, Akanksha Singh, Scott Pitnick, Mariana F. Wolfner, Steve Dorus
Summary: This study investigates the life history of Drosophila melanogaster sperm proteome and reveals substantial compositional changes after sperm are transferred to the female reproductive tract (FRT). Multiple seminal fluid proteins initially associate with sperm, but become undetectable after sperm storage. Female-derived proteins also associate with sperm immediately after mating, and contribute nearly 20% to the postmating sperm proteome after storage in the FRT. These female-derived proteins are enriched in processes associated with energy metabolism, suggesting their role in supporting sperm viability during the period between copulation and fertilization.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Limnology
Laura E. Sofen, Olga A. Antipova, Michael J. Ellwood, Naomi E. Gilbert, Gary R. LeCleir, Maeve C. Lohan, Claire Mahaffey, Elizabeth L. Mann, Daniel C. Ohnemus, Steven W. Wilhelm, Benjamin S. Twining
Summary: Nano- and picophytoplankton, which are major components of open-ocean ecosystems, have little known information about their trace metal contents. This study found that iron limitation reduced iron quotas in Ostreococcus lucimarinus cultures, while the iron contents in Prochlorococcus cultures varied with iron availability. Additionally, the availability of macronutrients and metals may play equally important roles in controlling metal contents of small eukaryotic phytoplankton in the open ocean.
LIMNOLOGY AND OCEANOGRAPHY LETTERS
(2022)
Editorial Material
Biochemistry & Molecular Biology
Yu-Ying Chen, Thomas V. O'Halloran
Summary: Zinc is crucial for living organisms, and it has been discovered that the ZNG1 family of GTPases function as metallochaperones, directly transferring zinc to and activating methionine aminopeptidases which play essential roles in protein modification during or after translation.
Editorial Material
Biochemistry & Molecular Biology
Stuart Wigby, Nora C. Brown, Irem Sepil, Mariana F. Wolfner
Summary: Some Drosophila melanogaster seminal fluid proteins are not exclusively expressed in the accessory glands, but also come from other male reproductive tissues.
INSECT MOLECULAR BIOLOGY
(2022)
Article
Genetics & Heredity
Dawn S. Chen, Andrew G. Clark, Mariana F. Wolfner
Summary: In a study on Drosophila, researchers found that inhibiting the activity of Tdc2 neurons in females resulted in a higher proportion of offspring from the first male. This suggests that octopaminergic/tyraminergic signaling plays a role in biased sperm usage in multiply-mated females, and highlights a new role for the female nervous system in postcopulatory sexual selection.
Review
Entomology
Melissa A. White, Mariana F. Wolfner
Summary: This article summarizes the studies on post-mating signaling events in the reproductive tract and digestive system of fruit flies, highlighting the role of male seminal fluid proteins. It also identifies incomplete parts of these pathways and proposes questions for future research.
Article
Biology
Snigdha Misra, Norene A. Buehner, Akanksha Singh, Mariana F. Wolfner
Summary: This study reveals that the SP-sperm binding pathway is not entirely male-derived and that female contributions are needed to regulate the levels of SP associated with sperm stored in their storage sites.
Article
Multidisciplinary Sciences
Sofie Y. N. Delbare, Sara Venkatraman, Kate Scuderi, Martin T. Wells, Mariana F. Wolfner, Sumanta Basu, Andrew G. Clark
Summary: This study used high-resolution time series RNA-sequencing data to explore the genetic architecture of female's response to SP in Drosophila melanogaster. The results showed that SP not only triggers the transcriptional switch in females after mating, but also affects metabolic gene regulatory network and neuronal gene regulatory network, especially the disruption of female's circadian rhythm.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Andrology
Mariana F. F. Wolfner, Susan S. S. Suarez, Steve Dorus
Summary: Interactions between spermatozoa and the female reproductive tract are complex and have been historically viewed as hostile. However, recent research reveals that these interactions are beneficial and essential for fertility.
Article
Anatomy & Morphology
Uwe Kierdorf, Santiago Gomez, Stuart R. Stock, Olga Antipova, Horst Kierdorf
Summary: We examined the pedicle bone of roe bucks that died during antler casting or around the rutting period. Pedicles obtained during antler casting showed signs of intense osteoclastic activity and the formation of an abscission line. Pedicles obtained during the rutting period were compact with newly formed secondary osteons exhibiting lower mineral density. This suggests mineral element competition between growing antlers and compacting pedicles, which is likely more severe in Capreolus capreolus due to their antler regrowth during a period of limited food and mineral supply.
JOURNAL OF ANATOMY
(2023)
Article
Genetics & Heredity
Hideyuki Iwahata, So-Youn Kim, Yuriko Iwahata, Nao Suzuki, Teresa K. Woodruff
Summary: Cancer therapy can cause ovarian insufficiency, and current methods for fertility preservation in female cancer patients are limited. This study investigates the potential protective effects of triiodothyronine (T3) on apoptosis induced by cisplatin and X-ray in mouse ovaries, as well as in breast cancer cells. The findings suggest that T3 is not effective as a fertoprotective agent and its candidacy for fertility preservation should be reconsidered.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2023)
Editorial Material
Biochemistry & Molecular Biology
Jonathon M. Thomalla, Mariana F. Wolfner
Article
Forestry
Magdalena Broda, Joseph E. Jakes, Luxi Li, Olga A. Antipova
Summary: This study investigates the mechanism of dimensional stabilization of archeological wood by organosilicon compounds using synchrotron-based X-ray fluorescence microscopy and nanoindentation. The results demonstrate that the organosilicons infiltrate wood cell walls and enhance their mechanical properties.
WOOD SCIENCE AND TECHNOLOGY
(2023)
