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Biochemistry & Molecular Biology
Laszlo Imre, Erfaneh Firouzi Niaki, Rosevalentine Bosire, Peter Nanasi, Peter Nagy, Zsolt Bacso, Nubar Hamidova, Yves Pommier, Albert Jordan, Gabor Szabo
Summary: This study explores the roles and interactions of polyamines (PAs) in the nucleus, specifically their effect on nucleosome stability. The results demonstrate that PAs, such as spermine, spermidine, and putrescine, have a strong destabilizing effect on nucleosomes in the millimolar concentration range. This effect is dependent on pH and salt concentration, and remains significant even at neutral pH. It was also found that PA treatment does not affect the integrity of genomic DNA. Furthermore, the study shows that PAs bind to DNA, as evidenced by the displacement of ethidium bromide.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Simon Obermeyer, Richard Stoeckl, Tobias Schnekenburger, Henna Kapoor, Thomas Stempfl, Uwe Schwartz, Klaus D. Grasser
Summary: In addition to transcriptional initiation, mRNA production is regulated during elongation, and various transcript elongation factors modulate the synthesis of mRNAs by RNA polymerase II (RNAPII) in the chromatin context. TFIIS is an elongation factor that stimulates the transcript cleavage activity of RNAPII, and its deficiency in Arabidopsis plants leads to increased sensitivity to heat stress and decreased expression of heat-induced genes. Further analysis reveals that TFIIS is required for proper RNAPII accumulation at specific nucleosomes and plays a role in reprogramming gene expression for establishing plant thermotolerance.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Cell Biology
Tadashi Nakagawa, Akane Morohoshi, Yuko Nagasawa, Makiko Nakagawa, Masaki Hosogane, Yasuhiro Noda, Toru Hosoi, Keiko Nakayama
Summary: Human SPT16 protein is ubiquitylated at the K674 residue by DCAF14-CRL4, inhibiting its binding to histone H3.1-H4. The K674R mutation of SPT16 affects cell proliferation and sensitivity to S phase stress.
Article
Biochemistry & Molecular Biology
Se Won Park, Jaehoon Kim, Sungryong Oh, Jeongyoon Lee, Joowon Cha, Hyun Sik Lee, Keun Il Kim, Daechan Park, Sung Hee Baek
Summary: Autophagy is a crucial pathway for maintaining cellular homeostasis under stress conditions. The regulation of autophagy genes through transcriptional and epigenetic control is essential. This study reveals that PHF20 plays a role in controlling the expression of autophagy genes by recognizing H3K36me2, emphasizing the importance of nuclear events in autophagy regulation.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Irina Bogolyubova, Dmitry Bogolyubov
Summary: DAXX is a multifunctional protein involved in various cellular processes, interacting with transcription factors and chromatin-remodeling proteins in the nucleus. Its specific function in mammalian early development as a chaperone of H3.3 supports proper development, impacting recruitment of ATRX and transcription of tandem repeats. Loss of DAXX results in decreased viability of early embryos.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Hongchang Li, Chaonan Li, Wenjing Zhai, Xin Zhang, Lei Li, Bo Wu, Biyue Yu, Pengfei Zhang, Jie Li, Chun-Ping Cui, Lingqiang Zhang
Summary: Autophagy is essential for neonatal energy homeostasis and survival. The deubiquitylase ubiquitin-specific peptidase 10 (USP10) destabilizes TP53 to regulate neonatal autophagy and viability.
Article
Multidisciplinary Sciences
Amy E. Loats, Samantha Carrera, Anna F. Fleming, Abigail R. E. Roberts, Alice Sherrard, Eneda Toska, Alexander J. Moorhouse, Kathryn F. Medler, Stefan G. E. Roberts
Summary: The study reveals that the lipidated protein BASP1 recruits cholesterol to the promoter region of target genes in the cell nucleus through a conserved interaction motif, and that BASP1's interaction with cholesterol is required for chromatin remodeling and the direction of transcription programs controlling cell differentiation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Editorial Material
Psychology, Biological
Marret K. Noordewier
Summary: Scientific misconduct can greatly affect scholars, especially junior ones, and the consequences can linger for years. Support is essential for them.
NATURE HUMAN BEHAVIOUR
(2023)
Article
Biology
Anastasiia L. Sivkina, Maria G. Karlova, Maria E. Valieva, Laura L. McCullough, Timothy Formosa, Alexey K. Shaytan, Alexey Feofanov, Mikhail P. Kirpichnikov, Olga S. Sokolova, Vasily M. Studitsky
Summary: Sivkina et al. characterized the interaction between S. cerevisiae histone chaperone FACT and the nucleosome core particle. They found that FACT adopts a more open geometry in the presence of Nhp6, and together they unfold nucleosomes. Nhp6 alters the structure of FACT and the properties of nucleosomes to support nucleosome unfolding.
COMMUNICATIONS BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Haruhiko Ehara, Tomoya Kujirai, Mikako Shirouzu, Hitoshi Kurumizaka, Shun-ichi Sekine
Summary: This study used cryo-electron microscopy to obtain structures of RNA polymerase II (RNAPII) passing through nucleosomes during gene transcription. The researchers also identified the roles of transcription elongation factors and histone chaperones in nucleosome disassembly and reassembly.
Article
Plant Sciences
Ricarda Hohner, Philip M. Day, Sandra E. Zimmermann, Laura S. Lopez, Moritz Kramer, Patrick Giavalisco, Viviana Correa Galvis, Ute Armbruster, Mark Aurel Schoettler, Peter Jahns, Stephan Krueger, Hans-Henning Kunz
Summary: This study unveils a stromal mechanism with NAD(H) specificity that aids in balancing chloroplast redox poise, potentially providing a promising target for manipulating plant photosynthesis.
Article
Multidisciplinary Sciences
Bradley J. Lukasak, Michelle M. Mitchener, Lingchun Kong, Barbara E. Dul, Cole D. Lazarus, Aarthi Ramakrishnan, Jizhi Ni, Li Shen, Ian Maze, Tom W. Muir
Summary: Recent research has identified a novel posttranslational modification (PTM) called serotonylation of glutamine-5 on histone H3 (H3Q5ser), which is associated with active transcription. However, this modification does not occur in constitutive heterochromatic regions and is hindered by higher-order chromatin structures. The activity of tissue transglutaminase 2 (TGM2) on chromatin is dictated by substrate accessibility rather than primary sequence determinants or preexisting PTMs.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Run Wang, Xuebing Li, Cuiyun Sun, Lin Yu, Dan Hua, Cuijuan Shi, Qian Wang, Chun Rao, Wenjun Luo, Zhendong Jiang, Xuexia Zhou, Shizhu Yu
Summary: Pontin is upregulated in gliomas and higher levels may predict worse prognosis. It is involved in regulating cell cycle and RNA processing, impacting gene expression and splicing through interaction with E2F1. The study sheds light on the potential application of Pontin as an anticancer target in glioma.
CELL DEATH & DISEASE
(2021)
Article
Multidisciplinary Sciences
Anfeng Luo, Jingwei Kong, Jun Chen, Xue Xiao, Jie Lan, Xiaorong Li, Cuifang Liu, Peng-Ye Wang, Guohong Li, Wei Li, Ping Chen
Summary: This study reveals the mechanism of direct collaboration between ubH2B and FACT at the nucleosome level. The researchers found that ubH2B impairs nucleosome stability and recruits FACT by enhancing its binding on the nucleosome. FACT preferentially binds and deposits H2A-ubH2B dimers to form an intact nucleosome. The stable altered nucleosome state obtained by ubH2B and FACT provides a key platform for gene transcription, as shown by genome-wide and time-course ChIP-qPCR analyses.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Xuezheng Wang, Yuantao Tang, Jiawei Xu, He Leng, Guojun Shi, Zaifeng Hu, Jiale Wu, Yuwen Xiu, Jianxun Feng, Qing Li
Summary: This study uncovers a new mechanism for parental histone recycling, revealing the important role of histone chaperone FACT and its N-terminus of the Spt16 subunit in budding yeast. The Spt16-N domain interacts with the replicative helicase MCM2-7, facilitating the formation of a critical ternary complex for efficient parental histone recycling and transfer to the lagging strand.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Oncology
Daria Fleyshman, Laura Prendergast, Alfiya Safina, Geraldine Paszkiewicz, Mairead Commane, Kelsey Morgan, Kristopher Attwood, Katerina Gurova
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Biochemistry & Molecular Biology
Laura Prendergast, Erin Hong, Alfiya Safina, Dante Poe, Katerina Gurova
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Oncology
Kristopher Attwood, Daria Fleyshman, Laura Prendergast, Geraldine Paszkiewicz, Angela R. Omilian, Wiam Bshara, Katerina Gurova
BREAST CANCER-TARGETS AND THERAPY
(2017)