Article
Chemistry, Multidisciplinary
Vadim Krivitsky, Adva Krivitsky, Valeria Mantella, Maya Ben-Yehuda Greenwald, Devanarayanan Siva Sankar, Jil Betschmann, Johannes Bader, Nicole Zoratto, Kento Schreier, Sarah Feiss, Dario Walker, Jorn Dengjel, Sabine Werner, Jean-Christophe Leroux
Summary: This study presents an electrochemical all-in-one device that can rapidly capture, load, and release extracellular vesicles (EVs). The device, composed of antibody-coated microstructured electrodes, effectively isolates EVs from various biofluids and improves EV loading with polyplexes. This portable device offers a lab-on-a-chip approach for efficient EV isolation and manipulation.
ADVANCED MATERIALS
(2023)
Review
Pharmacology & Pharmacy
Fanhua Kang, Yuanliang Yan, Yuanhong Liu, Qiuju Liang, Zhijie Xu, Wei Zhu, Abhimanyu Thakur
Summary: Exosomes, secreted by various cells, play a crucial role in cell-cell communication and have gained attention for their cargoes, including protein, lipids, DNA, and RNA. Among these cargoes, exosomal circular RNAs (circRNAs) have shown promising functional roles in cancer chemotherapeutic response by regulating gene expression. This review focuses on the important roles of exosomal circRNAs in cancer-associated signaling pathways and their potential effect on cancer therapeutic resistance.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Cell Biology
Artur Slomka, Miroslaw Kornek, William C. Cho
Summary: Extracellular vesicles, particularly exosomes or small EVs (sEVs), have been found to play a significant role in modulating the sensitivity of cancer cells to chemotherapeutic agents through the delivery of non-coding RNAs (ncRNAs). The mechanisms involved include translational repression of anti-oncogenes by exosomal microRNAs (miRs) and lack of translational repression of oncogenes by sponging of miRs through long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs). These processes ultimately result in increased proliferation and survival of cancer cells, enhanced metastasis, and resistance to apoptosis, leading to chemotherapy failures in different types of cancer patients.
Article
Engineering, Biomedical
Shi-Cong Tao, Ji-Yan Huang, Yuan Gao, Zi-Xiang Li, Zhan-Ying Wei, Helen Dawes, Shang-Chun Guo
Summary: OA is a common age-related degenerative joint disease with disturbance of ECM balance. CircRNA3503, increased after MT-induced cell sleep, acts as a sponge of hsa-miR-181c-3p and hsa-let-7b-3p to rescue IL-1 beta effects on OA. PLEL@circRNA3503-OE-sEVs show potential as an effective therapeutic strategy to prevent OA progression.
BIOACTIVE MATERIALS
(2021)
Review
Chemistry, Analytical
Marco Morani, Thanh Duc Mai, Zuzana Krupova, Guillaume van Niel, Pierre Defrenaix, Myriam Taverna
Summary: Extracellular vesicles (EVs) are a family of cell-derived membrane vesicles present in almost all body fluids, playing important roles in various patho-physiological conditions. Knowledge about the features of individual EVs is still in its infancy, but electrokinetically driven methods provide new trends for isolation and characterization of EVs.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Aleksandra Stec, Monika Targonska, Edyta Karkosinska, Monika Slowik, Agata Ploska, Leszek Kalinowski, Bartosz Wielgomas, Krzysztof Waleron, Jacek Jasiecki, Szymon Dziomba
Summary: The overproduction of proteins in Chinese hamster ovary (CHO) cells can induce the secretion of extracellular vesicles (EVs), potentially leading to the packaging of overexpressed proteins into exosomes. These findings are crucial for the manufacturing of therapeutic drugs in CHO expression systems, as there is a risk of product loss during downstream processing of culture medium and the use of exosomes as nanocarriers for therapeutic proteins.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Oncology
Weiqiang Tang, Min Xia, Yajie Liao, Yuan Fang, Gebo Wen, Jing Zhong
Summary: Exosomes are lipid bilayer extracellular vesicles that play critical roles in cancer initiation and progression, including TNBC. Tumor-derived exosomes have been found to be involved in various key processes of cancer, such as invasion, metastasis, angiogenesis, epithelial-mesenchymal transition, and drug resistance. Their dysregulation has been linked to the clinicopathological characteristics and prognosis of TNBC patients, making them a potential target for diagnosis and treatment.
Review
Medicine, Research & Experimental
Fang Wei, Yan Li
Summary: Exosomes, as important messengers, play a crucial role in regulating the tumor immune microenvironment. Noncoding RNAs encapsulated in exosomes have significant regulatory functions in the tumor immune microenvironment.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Medicine, Research & Experimental
Artur Bryja, Lukasz Zadka, Maryam Farzaneh, Mojtaba Zehtabi, Majid Ghasemian, Marta Dyszkiewicz-Konwinska, Paul Mozdziak, Maciej Zabel, Marzenna Podhorska-Okolow, Piotr Dziegiel, Hanna Piotrowska-Kempisty, Bartosz Kempisty
Summary: sEVs are membranous vesicles released by cells into the extracellular space, which have a complex and interdependent relationship with ncRNAs. By encapsulating therapeutic ncRNAs, sEVs can act as efficient carriers with the potential to traverse biological barriers and target specific cells. However, their application as therapeutic agents also raises concerns about potential risks and unintended impacts.
Review
Biochemistry & Molecular Biology
J. Makarova, A. Turchinovich, M. Shkurnikov, A. Tonevitsky
Summary: miRNAs are short RNA molecules that regulate multiple cellular processes through post-transcriptional gene silencing and mediate functional communication between cells in the extracellular space. While it is widely accepted that miRNA transfer between cells occurs via extracellular vesicles (EVs), there are also other carriers of cell-free miRNA.
TRENDS IN BIOCHEMICAL SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Dimitra Ioanna Lampropoulou, Evangelia Pliakou, Gerasimos Aravantinos, Dimitrios Filippou, Maria Gazouli
Summary: Exosomal ncRNAs play a significant role in CRC drug resistance, and this field has great research potential for discovering new biomarkers for therapy monitoring or reversing drug desensitization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Fereshteh Nazari-Khanamiri, Nima Abdyazdani, Reza Abbasi, Mahdi Ahmadi, Jafar Rezaie
Summary: Exosomes are membrane-bound nanoparticles that originated from eukaryotic cells and contribute to intracellular communication. Noncoding RNAs carried by exosomes play important roles in tumor angiogenesis, with some promoting tumor progression and others inhibiting angiogenesis. Understanding the roles of exosomal noncoding RNAs can aid in the development of exosome-based cancer therapies and biomarkers.
CELL BIOCHEMISTRY AND FUNCTION
(2023)
Review
Cell Biology
Yanhui Lu, Zhichao Zheng, Yunyi Yuan, Janak L. Pathak, Xuechao Yang, Lijing Wang, Zhitong Ye, William C. Cho, Mingtao Zeng, Lihong Wu
Summary: Oral cancer, primarily oral squamous cell carcinoma, remains a challenge to treat with high rates of metastasis. Exosomes play a crucial role in the tumorigenesis and progression of oral squamous cell carcinoma, and show potential for clinical application in treatment.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Lara Ottaviani, Rio P. Juni, Ricardo C. de Abreu, Marida Sansonetti, Vasco Sampaio-Pinto, Julie Halkein, Jana C. Hegenbarth, Nadja Ring, Kevin Knoops, Jordy M. M. Kocken, Carlos de Jesus, Auriane C. Ernault, Hamid El Azzouzi, Frank Ruhle, Serve Olieslagers, Hugo Fernandes, Lino Ferreira, Luca Braga, Monika Stoll, Diana S. Nascimento, Leon J. de Windt, Paula A. da Costa Martins
Summary: miR-200c-3p in extracellular vesicles secreted by cardiac myocytes plays a crucial role in the communication between cardiac cells and endothelial cells, affecting cardiac function and disease development.
Review
Cell Biology
Turner W. Seay, Zucai Suo
Summary: Liver cancer is a global health challenge and the third leading cause of cancer death worldwide. Hepatocellular carcinoma (HCC), the most common type of liver cancer, is associated with high morbidity and mortality rates. Recent studies have shown that extracellular vesicles (EVs) secreted by HCC cells play a critical role in HCC progression and metastasis by transferring oncogenic factors to surrounding cells. This review summarizes the roles of EVs and their cargos in HCC progression and metastasis, and identifies potential therapeutic targets for HCC treatment.
Review
Biochemistry & Molecular Biology
Norberto A. Guzman, Daniel E. Guzman
Summary: Protein biomarkers, specifically proteoforms, play a crucial role in disease diagnosis and prognosis. Immunoaffinity capillary electrophoresis (IACE) is an emerging diagnostic tool that can isolate and detect proteoform biomarkers from liquid biopsy, offering rapid and accurate results with reliability and reproducibility. Additionally, IACE has the potential to monitor the efficacy of therapeutic agents and improve early detection of medical conditions through advancements in telemedicine and artificial intelligence.