Article
Biochemistry & Molecular Biology
Jun Jiang, Ursula Kraneburg, Ulf Dornseifer, Arndt F. Schilling, Ektoras Hadjipanayi, Hans-Guenther Machens, Philipp Moog
Summary: This study investigated the effect of topical application of hypoxia preconditioned serum (HPS) on wound healing in mice. The results showed that HPS could accelerate wound healing, increase lymphatic vessels, and inhibit cell proliferation, but had no effect on vascularization.
Article
Biochemistry & Molecular Biology
Jun Jiang, Lynn Roeper, Sarah Alageel, Ulf Dornseifer, Arndt F. Schilling, Ektoras Hadjipanayi, Hans-Guenther Machens, Philipp Moog
Summary: This study demonstrated the osteogenic potential of hypoxia preconditioned serum (HPS) on human osteoblasts in vitro. HPS stimulated osteoblast proliferation, enhanced cell migration, upregulated osteoprotegerin (OPG), increased alkaline phosphatase (ALP) enzyme activity, and promoted extracellular deposition of calcium-phosphate. These findings suggest that HPS has the potential to accelerate bone regeneration by promoting osteogenic activity in human osteoblasts.
Article
Biochemistry & Molecular Biology
Philipp Moog, Jessica Hughes, Jun Jiang, Lynn Roeper, Ulf Dornseifer, Arndt F. F. Schilling, Hans-Guenther Machens, Ektoras Hadjipanayi
Summary: By replacing the autologous liquid components of hypoxia-preconditioned plasma (HPP) and serum (HPS) with different conditioning media, such as NaCl, PBS, Glucose 5%, and AIM V medium, the growth factor profile of HPP and HPS can be optimized, leading to changes in their angiogenic abilities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Marina Katsman, Aviva Azriel, Guy Horev, Yitzhak Reizel, Ben-Zion Levi
Summary: The study reveals the crucial role of N-VEGF as an autoregulatory arm of VEGF-A in angiogenesis and cell survival under hypoxic conditions.
Article
Biochemistry & Molecular Biology
Anna Wrona, Veronika Aleksandrovych, Tomasz Bereza, Pawel Basta, Anna Gil, Magdalena Ulatowska-Bialas, Malgorzata Mazur-Laskowska, Kazimierz Pitynski, Krzysztof Gil
Summary: Oxygen balance is crucial for reproductive function, and there is a relationship between the oviduct and uterine myoma. Patients with uterine myoma have different expressions of sex steroid receptors and an increased number of telocytes. Blood biochemical analyses reveal a higher progesterone level and lower AMH in patients with uterine myoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, Research & Experimental
Mariko Miyashita-Ishiwata, Malak El Sabeh, Lauren D. Reschke, Sadia Afrin, Mostafa A. Borahay
Summary: This study reveals an aberrant response of leiomyoma cells to hypoxia, characterized by increased cell proliferation and secretion of angiogenic factors. This abnormal response may contribute to the development and growth of leiomyoma.
Article
Biochemistry & Molecular Biology
Jun Jiang, Jannat Altammar, Xiaobin Cong, Lukas Ramsauer, Vincent Steinbacher, Ulf Dornseifer, Arndt F. Schilling, Hans-Guenther Machens, Philipp Moog
Summary: This study analysed the concentrations of chondrogenic growth factors in the blood-derived secretome of HPS and assessed the effects of HPS-10% and HPS-40% on human articular chondrocytes from osteoarthritic cartilage. It was found that HPS had higher concentrations of growth factors compared to NS, and HPS-10% promoted chondrocyte proliferation and improved chondrogenic phenotype. The findings suggest that HPS-10% has the potential to enhance conventional FCS-based ACI culture protocols.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Ajmal Ahmad, Mohd Imtiaz Nawaz
Summary: This review article summarizes the significant scientific contributions made in the past seven decades regarding the implications of vascular endothelial-derived growth factor (VEGF) in angiogenesis processes. It highlights the role of VEGF in pathological conditions and its potential as a therapeutic target for managing cancers and intraocular neovascular disorders. The article also emphasizes the need for further research and development of alternative strategies in the management of VEGF-mediated pathological angiogenesis. Additionally, it discusses the emerging field of therapeutic angiogenesis for the treatment of ischemic disorders.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Jun Jiang, Xiaobin Cong, Sarah Alageel, Ulf Dornseifer, Arndt F. Schilling, Ektoras Hadjipanayi, Hans-Guenther Machens, Philipp Moog
Summary: This study compared the lymphangiogenic potential of Hypoxia Preconditioned Serum (HPS) and Platelet-rich Plasma (PRP) and found higher concentrations of pro-lymphangiogenic growth factors in HPS. Both HPS and PRP significantly promoted the proliferation and migration of lymphatic endothelial cells (LECs), with the strongest effect observed with HPS-40% dilution. HPS-10% also showed the highest tube formation and ex vivo lymphatic sprouting.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Grazia Maugeri, Agata Grazia D'Amico, Salvatore Saccone, Concetta Federico, Daniela Maria Rasa, Rosario Caltabiano, Giuseppe Broggi, Salvatore Giunta, Giuseppe Musumeci, Velia D'Agata
Summary: The study shows that PACAP plays a role in regulating the hypoxic micro-environment, angiogenesis and EMT in GBM cells. By inhibiting specific pathways and molecules, PACAP can reduce invasiveness of GBM cells and interfere with VEGF release and vessel formation. This study provides new insights into the complex role of PACAP in GBM malignancy.
Article
Oncology
Baoyu Li, Ailin Liang, Yanling Zhou, Yihua Huang, Chenxi Liao, Xufang Zhang, Qimei Gong
Summary: The mechanism of the angiogenic potential in hypoxia-pretreated dental pulp stem cells (DPSCs) was investigated by studying the exosomes secreted by these cells. It was found that these exosomes promote angiogenesis via transfer of lysyl oxidase-like 2 (LOXL2).
EXPERIMENTAL CELL RESEARCH
(2023)
Article
Ophthalmology
Minsup Lee, Wendy Leskova, Randa S. Eshaq, Norman R. Harris
Summary: The study revealed that METH administration resulted in increased retinal hypoxia, HIF-1α, VEGFa, and angiogenesis.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Multidisciplinary Sciences
Adrian Moure, Guillermo Vilanova, Hector Gomez
Summary: This study investigates the complex interactions between interstitial flow and the affinity for matrix binding of different chemokine isoforms using a computational model. The results demonstrate that changing the matrix affinity of the chemokine isoform can invert the effect of interstitial flow on angiogenesis. These findings provide insight into the intricate mechanisms of angiogenesis in the tumor microenvironment.
SCIENTIFIC REPORTS
(2022)
Review
Physiology
Pierre Lemieux, Olivier Birot
Summary: Hypoxia, defined as reduced oxygen availability, triggers complex angio-adaptive responses in skeletal muscle tissue, resulting in the growth, stabilization, or regression of muscle capillaries, known as angio-adaptation. Angio-adaptive molecules released in the circulation act on distant tissues. The combined potency of ambient hypoxia and exercise in generating a cellular hypoxic stress in skeletal muscle is discussed, highlighting the molecular complexity and identifying gaps in knowledge in this field.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Simon Klepper, Susan Jung, Lara Dittmann, Carol Geppert, Arnd Hartmann, Nicole Beier, Regina Trollmann
Summary: Experimental data indicate that rhGH and rhEPO have complementary neuroprotective actions in neonatal hypoxic brain injury. The study also suggests that rhGH and rhEPO mediate stabilization of the blood-brain barrier and promote regenerative vascular effects in the injured brain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)