Article
Biochemistry & Molecular Biology
Jun Jiang, Ursula Kraneburg, Ulf Dornseifer, Arndt F. Schilling, Ektoras Hadjipanayi, Hans-Guenther Machens, Philipp Moog
Summary: This study investigated the effect of topical application of hypoxia preconditioned serum (HPS) on wound healing in mice. The results showed that HPS could accelerate wound healing, increase lymphatic vessels, and inhibit cell proliferation, but had no effect on vascularization.
Review
Biochemistry & Molecular Biology
Emerito Carlos Rodriguez-Merchan
Summary: This article reviews recent findings on the molecular mechanisms of bone healing and presents various biological alternatives for treating recalcitrant nonunions, such as using morin, BMP9, and leptin. It also discusses factors that impact bone regeneration, including inhibition of estrogen receptor signaling, smoking-induced inflammatory responses, and delayed fracture healing in diabetic animals due to BMP6 deficiency. The combination of bioceramics and expanded autologous human mesenchymal stem cells from bone marrow is highlighted as a promising alternative for treating nonunions that do not respond well to traditional treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jun Jiang, Xiaobin Cong, Sarah Alageel, Ulf Dornseifer, Arndt F. Schilling, Ektoras Hadjipanayi, Hans-Guenther Machens, Philipp Moog
Summary: This study compared the lymphangiogenic potential of Hypoxia Preconditioned Serum (HPS) and Platelet-rich Plasma (PRP) and found higher concentrations of pro-lymphangiogenic growth factors in HPS. Both HPS and PRP significantly promoted the proliferation and migration of lymphatic endothelial cells (LECs), with the strongest effect observed with HPS-40% dilution. HPS-10% also showed the highest tube formation and ex vivo lymphatic sprouting.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jun Jiang, Jannat Altammar, Xiaobin Cong, Lukas Ramsauer, Vincent Steinbacher, Ulf Dornseifer, Arndt F. Schilling, Hans-Guenther Machens, Philipp Moog
Summary: This study analysed the concentrations of chondrogenic growth factors in the blood-derived secretome of HPS and assessed the effects of HPS-10% and HPS-40% on human articular chondrocytes from osteoarthritic cartilage. It was found that HPS had higher concentrations of growth factors compared to NS, and HPS-10% promoted chondrocyte proliferation and improved chondrogenic phenotype. The findings suggest that HPS-10% has the potential to enhance conventional FCS-based ACI culture protocols.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
Jos Crush, Ali Hussain, K. T. M. Seah, Wasim S. Khan
Summary: Biomaterials play an important role in bone tissue regeneration, especially in cases of trauma, infection, and congenital abnormalities. Bioactive glasses can bond with host bone and induce bone and blood vessel growth, with their composition manipulated to maximize osteogenesis and angiogenesis, providing a structure that resists physiological stresses. Accurate methods for studying and quantifying osteogenesis and angiogenesis in bioactive glass experiments are crucial for identifying potential future clinical applications.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Philipp Moog, Jessica Hughes, Jun Jiang, Lynn Roeper, Ulf Dornseifer, Arndt F. F. Schilling, Hans-Guenther Machens, Ektoras Hadjipanayi
Summary: By replacing the autologous liquid components of hypoxia-preconditioned plasma (HPP) and serum (HPS) with different conditioning media, such as NaCl, PBS, Glucose 5%, and AIM V medium, the growth factor profile of HPP and HPS can be optimized, leading to changes in their angiogenic abilities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Dentistry, Oral Surgery & Medicine
A. M. Decker, M. Matsumoto, J. T. Decker, A. Roh, N. Inohara, J. Sugai, K. Martin, R. Taichman, D. Kaigler, L. D. Shea, G. Nunez
Summary: Regeneration of alveolar bone following tooth extraction is crucial for restoring healthy function. The TAM family of receptor tyrosine kinases, particularly Mertk, has been identified as a therapeutic target to enhance bone regeneration by upregulating Wnt signaling. Experimental evidence suggests that a Mertk-targeted inhibitor can improve bone regeneration. Therefore, interventions targeting the TAM receptor signaling pathway, specifically Mertk, may have therapeutic benefits for bone regeneration.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Engineering, Multidisciplinary
Chengzhong Xu, Tao Lin, Xiaoliang Zhao, Yanchang Gan, Jianxing Huang, Jie Zhang, Haibin Zheng, Chunyi Pu, Rurong Lin, Bing Yan, Guoju Hu, Qiaolan Liu, Bo Yu, Songjian Li, Honghao Hou
Summary: In this study, a hypoxic-mimetic 3D porous hydrogel was developed to mimic the hypoxic and acidic microenvironment at the tendon-to-bone interface. The hydrogel can release a hypoxia agent, which upregulates HIF-1α and promotes angiogenesis and bone formation. It also reduces oxidative stress and inflammation, leading to better integration of the tendon-to-bone interface.
COMPOSITES PART B-ENGINEERING
(2023)
Article
Orthopedics
Tianyou Kan, Zihao He, Jingke Du, Mingming Xu, Junqi Cui, Xuequan Han, Dake Tong, Hanjun Li, Mengning Yan, Zhifeng Yu
Summary: Through animal experiments and in vitro cell experiments, it was found that irisin can promote bone fracture healing by enhancing osteogenesis and angiogenesis, which is of great significance in understanding the interaction between muscle and bone during fracture healing.
JOURNAL OF ORTHOPAEDIC TRANSLATION
(2022)
Review
Metallurgy & Metallurgical Engineering
Ning Wang, Shude Yang, Huixin Shi, Yiping Song, Hui Sun, Qiang Wang, Lili Tan, Shu Guo
Summary: This review provides an account of the specific mechanisms by which magnesium alloys promote bone healing, including immunomodulation, angiogenesis, osteogenesis, and regulation of osteoclast function. It highlights the regulation of magnesium alloys on various cells involved in bone healing and summarizes the signaling pathways involved. This review also supports the future investigation and application of magnesium alloys in orthopedic fields.
JOURNAL OF MAGNESIUM AND ALLOYS
(2022)
Article
Biotechnology & Applied Microbiology
Xingzhi Zhou, Jiayu Chen, Hangxiang Sun, Fangqian Wang, Yikai Wang, Zengjie Zhang, Wangsiyuan Teng, Yuxiao Ye, Donghua Huang, Wei Zhang, Xianan Mo, An Liu, Peng Lin, Yan Wu, Huimin Tao, Xiaohua Yu, Zhaoming Ye
Summary: This study aimed to emulate natural bone healing cascades by coupling angiogenesis and osteogenesis processes through a hybrid dual growth factor delivery system. Results showed that the hybrid hydrogel notably enhanced vasculature formation and osteogenic differentiation, providing a facile and potent alternative for bone regeneration.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Lingli Ding, Song Gu, Bingyu Zhou, Min Wang, Yage Zhang, Siluo Wu, Hong Zou, Guoping Zhao, Zhao Gao, Liangliang Xu
Summary: Fractures have a negative impact on an individual's quality of life, but Ginsenoside Compound K (CK) found in Ginseng has shown potential for promoting bone regeneration and angiogenesis. This study found that CK can promote osteogenic differentiation of rat bone marrow mesenchymal stem cells and increase tube formation capacity in human umbilical vein endothelial cells. In a rat open femoral fracture model, CK was found to improve fracture repair and increase the formation of H type vessel in the fracture callus.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Pavitra K. Jadaun, Shuang Zhang, Marijke Koedam, Jeroen Demmers, Suvro Chatterjee, Johannes P. van Leeuwen, Bram C. van der Eerden
Summary: The research indicates that MUC1 plays a critical role in osteoblastogenesis under hypoxic conditions, regulating the protein composition and influencing the osteogenic and angiogenic potentials of bone cells.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Engineering, Environmental
Pengfei Xia, Meilin Yu, Jianjun Fang, Yan Gong, Shifeng Yan, Guifei Li, Dong Chen, Jingbo Yin
Summary: A novel hierarchically porous and hollow microcarrier containing multicellular spheroids and adhesive cells has been developed to promote the regeneration of vascular bone tissues without causing side effects. The multicellular spheroids serve as the Hypoxia-engine to increase the expression of osteogenic and angiogenic factors, while the adhesive cells facilitate the development of new bones. The microcarriers containing Hypoxia-engine have shown efficacy comparable to standard clinical grafts (Geistlich Bio-Oss) in regenerating alveolar bone tissues.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Engineering, Biomedical
Que Bai, Caiyun Zheng, Na Sun, Wenting Chen, Qian Gao, Jinxi Liu, Fangfang Hu, Tong Zhou, Yanni Zhang, Tingli Lu
Summary: This study presents an oxygen-releasing hydrogel that promotes cell survival, migration, and tube formation under hypoxic conditions, and facilitates tissue repair by reducing inflammation, increasing angiogenesis, and collagen deposition.
ACTA BIOMATERIALIA
(2022)
Article
Biochemistry & Molecular Biology
Jun Jiang, Ursula Kraneburg, Ulf Dornseifer, Arndt F. Schilling, Ektoras Hadjipanayi, Hans-Guenther Machens, Philipp Moog
Summary: This study investigated the effect of topical application of hypoxia preconditioned serum (HPS) on wound healing in mice. The results showed that HPS could accelerate wound healing, increase lymphatic vessels, and inhibit cell proliferation, but had no effect on vascularization.
Article
Biochemistry & Molecular Biology
Nele Wagener, Wolfgang Lehmann, Lukas Weiser, Katharina Jaeckle, Pietro Di Fazio, Arndt F. Schilling, Kai O. Boeker
Summary: Attention deficit hyperactivity disorder (ADHD) is a common mental disorder in children, young people and adults. The abuse of ADHD-related drugs is increasing, and this study found that three cognitive enhancers negatively influence the differentiation and migration of bone-forming cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jun Jiang, Xiaobin Cong, Sarah Alageel, Ulf Dornseifer, Arndt F. Schilling, Ektoras Hadjipanayi, Hans-Guenther Machens, Philipp Moog
Summary: This study compared the lymphangiogenic potential of Hypoxia Preconditioned Serum (HPS) and Platelet-rich Plasma (PRP) and found higher concentrations of pro-lymphangiogenic growth factors in HPS. Both HPS and PRP significantly promoted the proliferation and migration of lymphatic endothelial cells (LECs), with the strongest effect observed with HPS-40% dilution. HPS-10% also showed the highest tube formation and ex vivo lymphatic sprouting.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Tanita Man, Jun Jiang, Manuela Schulz, Haydar Kuekrek, Julia Betzl, Hans-Guenther Machens, Holger C. Erne, Philipp Moog
Summary: The study showed that surgical experience has a significant impact on tactile sensibility for surgeons and non-surgeons. Thinner gloves exhibited similar tactile sensitivity to bare hands in both groups. Double-gloving had a negative impact on sensibility, while undersized gloves showed better sensitivity.
Article
Biochemistry & Molecular Biology
Jun Jiang, Jannat Altammar, Xiaobin Cong, Lukas Ramsauer, Vincent Steinbacher, Ulf Dornseifer, Arndt F. Schilling, Hans-Guenther Machens, Philipp Moog
Summary: This study analysed the concentrations of chondrogenic growth factors in the blood-derived secretome of HPS and assessed the effects of HPS-10% and HPS-40% on human articular chondrocytes from osteoarthritic cartilage. It was found that HPS had higher concentrations of growth factors compared to NS, and HPS-10% promoted chondrocyte proliferation and improved chondrogenic phenotype. The findings suggest that HPS-10% has the potential to enhance conventional FCS-based ACI culture protocols.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Philipp Moog, Jessica Hughes, Jun Jiang, Lynn Roeper, Ulf Dornseifer, Arndt F. F. Schilling, Hans-Guenther Machens, Ektoras Hadjipanayi
Summary: By replacing the autologous liquid components of hypoxia-preconditioned plasma (HPP) and serum (HPS) with different conditioning media, such as NaCl, PBS, Glucose 5%, and AIM V medium, the growth factor profile of HPP and HPS can be optimized, leading to changes in their angiogenic abilities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, General & Internal
Katharina Jaeckle, Takashi Yoshida, Kira Neigefink, Marc-Pascal Meier, Mark-Tilmann Seitz, Thelonius Hawellek, Gabriela von Lewinski, Paul Jonathan Roch, Lukas Weiser, Arndt F. Schilling, Wolfgang Lehmann
Summary: Iliosacral joint immobilization causes task-dependent deviations in walking, such as increased plantarflexor force and altered joint movements. Patients experience significant mobility limitation after iliosacral screw fixation.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Medicine, General & Internal
Jonathan Ehmig, Guenther Engel, Joachim Lotz, Wolfgang Lehmann, Shahed Taheri, Arndt F. F. Schilling, Ali Seif Amir Hosseini, Babak Panahi
Summary: This review provides an overview of the current state of magnetic resonance imaging (MRI) in osteoarthritis (OA), with a focus on the knee joint. It discusses the use of MRI in evaluating OA severity and progression, as well as new developments in compositional MRI techniques and semiquantitative joint assessment. The potential impact of techniques to reduce acquisition times and deep learning in MR imaging for OA is also discussed.
Article
Chemistry, Medicinal
Marina Komrakova, Arndt Friedrich Schilling, Wolfgang Lehmann, Veselin Vasilev, Katerina Georgieva, Fanka Gerginska, Nikolay Boyadjiev, Slavi Delchev
Summary: The effects of combination treatments using selective androgen receptor modulators (SARMs) ostarine (OST) or ligandrol (LIG) with treadmill exercise (TE) were studied in healthy adult rats. The results showed that while SARM treatments had no clear bone benefit, OST + TE improved bone structure, while LIG + TE had unfavorable effects. Additionally, SARM treatments altered serum cholesterol and lipoprotein levels, indicating potential side effects. Therefore, attention should be paid to possible side effects when using SARMs.
Article
Biochemistry & Molecular Biology
Kai Oliver Boeker, Linda Gaetjen, Christopher Doelle, Katarina Vasic, Shahed Taheri, Wolfgang Lehmann, Arndt Friedrich Schilling
Summary: In this study, a novel coating technology was used to modify the surface of stainless-steel plates for trauma surgery. The modified plates showed good biocompatibility and reduced cell adhesion, allowing for easier removal without compromising implant function. This new surface coating method offers potential benefits in terms of cost-effectiveness and patient wellbeing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)