Journal
PLANTS-BASEL
Volume 9, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/plants9080997
Keywords
sterilization; shoot initiation; shoot proliferation; rooting; genetic fidelity assessment
Categories
Funding
- Forestry Public Welfare Industry Research of China [201404116]
- National Natural Science Foundation of China [31670601]
- Forestry Science and Technology Innovation of Guangdong Province [2014KJCX006, 2017KJCX023]
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Magnolia lucida(Magnoliaceae) is classified as an endangered species by the International Union for Conservation of Nature. It has high commercial value owing to its attractive tree shape and flowers. We adopted an excellent genotype ofM. lucidaas the parent material and established a mini-cut orchard through grafting to provide trunk shoots explants over the long-term. Optimal sterilization was achieved using a combination of 75% ethanol for 30 s, one percent benzalkonium bromide for five minutes, and 0.1% mercuric chloride for five minutes. Modified Murashige and Skoog medium (ML) was the optimal medium for the growth ofM. lucida. Addition of one mg/L of 6-benzyl adenine (BA) and 0.05 mg/L of alpha-naphthaleneacetic acid (NAA) to the medium increased the shoot induction rate to 95.56%, and the ML medium containing 0.4 mg/L BA and 0.04 mg/L NAA achieved the maximum multiplication rate (284.56%). Dark treatment for seven days, followed by continuous light treatment could better resolve the challenge of difficult rooting inM. lucidaplants. Using random amplified polymorphic DNA and inter simple sequence repeat markers, we confirmed the genetic uniformity and stability of the regenerated plants. Our protocol should be helpful for the propagation and conservation of this endangered plant.
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