Chemical and Enantioselective Analysis of the Leaf Essential Oil from Piper coruscans Kunth (Piperaceae), a Costal and Amazonian Native Species of Ecuador

In the present study, an essential oil was distilled from the leaves ofPiper coruscansKunth, a native Amazonian species belonging to the family Piperaceae and quite common in Ecuador. The chemical analysis was performed by GC-MS (qualitative) and GC-FID (quantitative), on polar and non-polar columns, detecting a total of 58 compounds of which 52 were identified. All the identified compounds were quantified. The essential oil was mainly constituted of sesquiterpenes (54.1-55.0%) and oxygenated sesquiterpenoids (32.5-33.6%), the major constituents being: (E)-beta-caryophyllene (24.1-25.0%), alpha-humulene (11.6-12.0%), caryophyllene oxide (9.3-10.9%), linalool (4.5-5.2%), humulene epoxide II (3.6-4.1%), (E)-nerolidol (3.7-4.0%), alpha-copaene (3.7-3.9%), alpha-muurolol (3.4-3.7%), alpha-selinene (3.4-3.5%), beta-selinene (3.1-3.3%), and one undetermined oxygenated sesquiterpenoid (3.1-3.3%). The aqueous phase (hydrolate) of the distillation process was also submitted to chemical analysis, showing linalool as the main organic compound in solution, with a concentration of 12.3-15.7 mg/100 mL. The essential oil was than analyzed for the enantiomeric distribution of its monoterpene constituents, affording the following enantiomeric excesses in two beta-cyclodextrin-based enantioselective columns: (1S,5S)-(-)-alpha-pinene (60.0-69.6%), (1S,5S)-(-)-beta-pinene (5.2-7.2%), (R)-(-)-alpha-phellandrene (72.5-78.2%), (R)-(+)-limonene (28.6%) and (R)-(-)-linalool (1.8-3.1%).