Article
Biochemistry & Molecular Biology
Don M. Matshazi, Cecil J. Weale, Rajiv T. Erasmus, Andre P. Kengne, Saarah F. G. Davids, Shanel Raghubeer, Glenda M. Davison, Tandi E. Matsha
Summary: The study on novel microRNAs in an African population revealed that some microRNAs are dysregulated in high blood pressure status, suggesting their potential as biomarker targets for diagnosis or treatment of hypertension.
MOLECULAR BIOLOGY REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Yi Cao, Yuning Lan, Hongjuan Huang, Shouhui Wei, Xiangju Li, Ying Sun, Ruolin Wang, Zhaofeng Huang
Summary: The green foxtail weed in China has developed a strong resistance to the herbicide nicosulfuron due to excessive use, with a 35.8-fold resistance observed in the R376 population. Resistance is caused by a mutation in the ALS gene and enhanced metabolism, involving ten candidate genes including ABC transporters, P450 enzymes, UGT, and GST.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Andrea N. Decarlo, Joseph Parrish, Jasmine D. Quarles, Nathan M. Long, Scott L. Pratt
Summary: This study aimed to assess the abundance of specific interferon-stimulated genes (ISG) and microRNA (miRNA) on day 18 of gestation in cattle. The results showed that certain ISG may serve as early indicators of pregnancy in cattle, while miRNA abundance does not.
Review
Biotechnology & Applied Microbiology
Tarana Siddika, Ilka U. Heinemann
Summary: MiRNAs are small non-coding RNAs that regulate posttranscriptional gene expression by interacting with target mRNAs. The finely controlled biogenesis, target recognition, and degradation of miRNAs play crucial roles in maintaining cell proliferation, growth, differentiation, and apoptosis. MiRNAs have emerged as potential biomarkers for disease diagnosis, with their expression and activity varying in different contexts such as developmental stages, tissues, and responses to external stimuli.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biology
Setu Chakraborty, Nardos T. Woldemariam, Tina Visnovska, Matthew L. Rise, Danny Boyce, Javier Santander, Rune Andreassen
Summary: This study characterized the miRNA repertoire of lumpfish using small RNA sequencing and miRDeep analysis. A total of 443 unique mature miRNAs were identified, with enrichment of specific miRNAs in particular organs and developmental stages. These miRNAs may play a role in regulating organ and developmental stage-specific functions in lumpfish.
Article
Biochemistry & Molecular Biology
Jianxiang Zhang, Cheng Xu, Kangwei Liu, Yaoqinq Li, Mengna Wang, Lian Tao, Hengxiu Yu, Chao Zhang
Summary: Plants may suffer DNA damage under extreme environmental conditions, leading to chromosomal abnormalities or cell death if not repaired rapidly and accurately. Organisms have evolved various DNA repair mechanisms, including the regulation of DNA damage repair by miRNAs targeting gene transcription. Specific miRNAs are found to be related to DNA repair processes, providing valuable insights for further functional studies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Jessica Maria Abbate, Alessia Giannetto, Francesca Arfuso, Barbara Brunetti, Giovanni Lanteri
Summary: This study found that the expression of certain MiRNAs is dysregulated in canine mammary tumors, and these MiRNAs may serve as candidate biomarkers to predict prognosis, as their dysregulation may lead to alterations in key pathways involved in cancer development.
Article
Veterinary Sciences
Janne G. Lyngby, Marta Godia, Louise Brogaard, Annemarie T. Kristensen, Merete Fredholm, Ellen Skancke, Joanna Morris, Nana Dupont, Silke Salavati Schmitz, David Argyle, Armand Sanchez, Charlotte R. Bjornvad, Susanna Cirera, Lise N. Nielsen
Summary: This study found that specific microRNAs in feces and serum of dogs can be used as noninvasive biomarkers to differentiate gastrointestinal cancer from chronic inflammatory enteropathy. MiR-451 and miR-223 showed the best diagnostic performance, and combining them slightly improved the diagnostic effectiveness.
JOURNAL OF VETERINARY INTERNAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Ana Clara Lagazzi Cressoni, Leticia B. C. Penariol, Cristiana Carolina Padovan, Maristela D. Orellana, Julio Cesar Rosa-e-Silva, Omero Benedicto Poli-Neto, Rui Alberto Ferriani, Claudia Cristina Paro de Paz, Juliana Meola
Summary: Menstrual blood mesenchymal stem cells (MenSCs) are a noninvasive source with potential clinical applications in regenerative medicine. In endometriotic MenSCs, miRNAs are involved in regulating important cellular processes. However, the miRNA biogenesis pathway in endometriotic MenSCs has not been studied.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Wei Li, Pingping He, Xingzhi Zhang, Junliang Guan, Yongxian Chen, Li Zhang, Bin Zhang, Yusi Zheng, Xin Li, Qingsong He, Longcheng Liu, Chang Yuan, Pinyuan Wei, Jinxia Peng
Summary: This study performed high-throughput sequencing to reveal the miRNA expression profile in the gonads of white Pacific shrimp and identified sex-related differentially expressed miRNAs and KEGG pathways. The results of this study are of great significance for understanding the regulatory mechanism of gonadal differentiation in white Pacific shrimp.
Article
Biochemistry & Molecular Biology
Patrick H. D. Petersen, Joanna Lopacinska-Jorgensen, Claus K. Hogdall, Estrid V. Hogdall
Summary: This study aimed to identify microRNAs that are stably expressed in the plasma of HGSOC and benign ovarian tumor patients. RNA was isolated from plasma samples of 60 HGSOC and 48 benign patients, and RT-qPCR was performed to analyze the stability of 40 microRNAs and 8 controls. It was found that hsa-miR-126-3p and hsa-miR-23a-3p were the most stable miRNAs in HGSOC samples, while hsa-miR-191-5p and hsa-miR-27a-3p were the most stable in benign samples.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Lin-Jie Yang, Li Gao, Yi-Nan Guo, Zi-Qian Liang, Dong-Ming Li, Yu-Lu Tang, Yi-Hong Liu, Wan-Jing Gao, Jing-Jing Zeng, Lin Shi, Kang-Lai Wei, Gang Chen
Summary: This study identified the expression pattern of miR-141-3p and its potential target genes in endometrial carcinoma, as well as its correlation with prognosis. The overexpression of miR-141-3p may play a crucial role in the carcinogenesis of endometrial carcinoma and be a risk factor for patient prognosis.
Article
Biotechnology & Applied Microbiology
Henk P. Roest, Jan N. M. IJzermans, Luc J. W. van der Laan
Summary: Extracellular microRNAs released from cells into biofluids are considered promising biomarkers for diagnostic and prognostic purposes. Different RNA isolation methods show variations in miRNA recovery and co-purification of RT-qPCR inhibitory compounds, with heparin being the major inhibitor and biliverdin from bile also potentially interfering with detection.
Article
Dermatology
Mingwang Zhang, Zhikuan Xia, Xin Yang, Junhong Ao, Rongya Yang
Summary: This study explored the miRNA immune response in THP-1 macrophages following exposure to Trichosporon asahii in vitro, showing that miR-342-3p may promote inflammatory responses by negatively regulating Dectin-1. KEGG pathway analysis revealed that up-regulated mRNAs were mainly involved in immune-related pathways.
Article
Genetics & Heredity
Jiefei Nai, Tieming Ma, Yingjie Liu, Yunwei Zhou
Summary: In this study, high-throughput sequencing was used to identify known and new miRNAs in the Chrysanthemum variety Niu 9717. The miRNAs were found to form miRNA-target pairs with mRNAs in the root and leaf, and were involved in pathways related to hormone signaling during plant growth and development.
Article
Plant Sciences
Joseph L. Pegler, Jackson M. J. Oultram, Duc Quan Nguyen, Christopher P. L. Grof, Andrew L. Eamens
Summary: Studies have found that under Cd stress, the drb1 mutant exhibits the highest tolerance, while the drb2 mutant is the most sensitive. DRB1 is the primary DRB protein required for six of the seven Cd-responsive miRNAs, while DRB2 is essential for the production of miR396.
Article
Plant Sciences
Joseph L. Pegler, Jackson M. J. Oultram, Christopher P. L. Grof, Andrew L. Eamens
Summary: The microRNA399/PHOSPHATE2 expression module plays a key role in Arabidopsis' response to phosphate stress. Altering miR399 abundance in Arabidopsis under salt stress conditions resulted in changes in the expression of phosphate transporter genes and enhanced phosphate translocation from roots to shoots, potentially aiding in maintaining essential biological processes or mounting an adaptive response to salt stress.
Review
Biochemistry & Molecular Biology
Jackson M. J. Oultram, Joseph L. Pegler, Timothy A. Bowser, Luke J. Ney, Andrew L. Eamens, Christopher P. L. Grof
Summary: This review explores the potential of Cannabis to produce secondary compounds with medical benefits, highlighting the advancements in plant science and pharmacology. It also draws parallels to research on opium poppy as a possible future direction for Cannabis plant biology research beyond its well-known constituents in medical biology.
Article
Agronomy
Joseph L. Pegler, Duc Quan Nguyen, Jackson M. J. Oultram, Christopher P. L. Grof, Andrew L. Eamens
Summary: Alteration of miR396 expression module in Arabidopsis is central to the unique phenotypic and physiological responses to salt stress, as demonstrated by molecular and phenotypic analyses in this study.
Article
Biochemistry & Molecular Biology
Thu Hien Nguyen, Duc Quan Nguyen, Lien Nguyen Thi Kim, Thanh Ngan Nguyen Thi, Thi Phuong Mai Nguyen, Ngoc Dung Tran, Huy Hoang Nguyen
Summary: This study aimed to identify AR gene mutations associated with male DSD in Vietnamese patients. The results from whole exome sequencing and in silico analysis revealed four novel AR mutations that are pathogenic and responsible for the development of AIS.
Review
Endocrinology & Metabolism
Leah Calvert, Mark P. Green, Geoffry N. De Iuliis, Matthew D. Dun, Brett D. Turner, Bradley O. Clarke, Andrew L. Eamens, Shaun D. Roman, Brett Nixon
Summary: Per-fluoroalkyl and polyfluoroalkyl substances (PFAS) are widely used synthetic fluorinated chemicals that have become ubiquitous environmental contaminants. PFAS exposure has been associated with negative reproductive outcomes in males, including testicular dysfunction and reduced semen quality. However, inconsistent findings have made it challenging to definitively link specific PFAS exposure to these health effects. Further research and the development of reliable biological models are needed to better understand the mechanisms of PFAS-mediated damage and mitigate the impact of PFAS contamination.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Multidisciplinary Sciences
Natalie A. Trigg, David A. Skerrett-Byrne, Jacinta H. Martin, Geoffry N. De Iuliis, Matthew D. Dun, Shaun D. Roman, Andrew L. Eamens, Brett Nixon
Summary: This study reports the proteomic effects of in vivo exposure to acrylamide on the epithelial cell population of the mouse epididymis. The results show that acrylamide can alter the expression of proteins in the epididymal epithelial cells and impact the sperm small non-coding RNA profile and embryo development.
Article
Plant Sciences
Louisa Matthew, Melquiades E. C. Reyes, Christopher W. G. Mann, Alasdair W. McDowall, Andrew L. Eamens, Bernard J. Carroll
Summary: Most flowering plant species have at least two copies of the DEFECTIVE EMBRYO AND MERISTEMS (DEM) gene, with mutations in the S1DEM1 locus in tomato causing shoot and root meristem defects. The characterization of S1DEM1 gene function in tomato revealed its role in various stages of vegetative development.
Article
Biochemistry & Molecular Biology
Jackson M. J. Oultram, Joseph L. Pegler, Greg M. Symons, Timothy A. Bowser, Andrew L. Eamens, Christopher P. L. Grof, Darren J. Korbie
Summary: This study assessed the genetic diversity of 10 Cannabis varieties through reduced representation shotgun sequencing, providing fundamental knowledge for the development of the pharmaceutical and recreational markets. The analysis identified 172 SNPs associated with genetic structure, which could be used to design a targeted resequencing panel for screening genetic relationships and scientific classification of Cannabis plants.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
E. Johnston, S. Okada, C. M. Gregg, A. C. Warden, V. Rolland, V. Gillespie, K. Byrne, M. L. Colgrave, A. L. Eamens, R. S. Allen, C. C. Wood
Summary: The goal of transferring a bacterial nitrogen fixation pathway into plants to reduce chemical fertilizer use on crops has been achieved in this study. The structural components of Fe-nitrogenase proteins were successfully engineered into plant mitochondria and formed a functional complex. This marks the first step towards engineering an alternative nitrogenase into crops.
PLANT MOLECULAR BIOLOGY
(2023)
Review
Plant Sciences
Joseph L. L. Pegler, Jackson M. J. Oultram, Christopher W. G. Mann, Bernard J. J. Carroll, Christopher P. L. Grof, Andrew L. L. Eamens
Summary: The miniature inverted-repeat transposable elements (MITEs) have contributed significantly to expanding the miRNA repertoire of angiosperms by transposing within gene-rich regions and producing noncoding RNA (ncRNA) that can be processed into miRNA.
Review
Biochemistry & Molecular Biology
Christopher W. G. Mann, Anne Sawyer, Donald M. Gardiner, Neena Mitter, Bernard J. Carroll, Andrew L. Eamens
Summary: The conflict between the need to conserve natural ecosystems and to feed an expanding population is driving the demand for more ecologically sustainable pathogen control measures. RNA-based biopesticides offer an eco-friendly alternative to conventional fungicides for crop protection. The development of pathogen-specific RNAi-inducing sprays and the understanding of gene silencing pathways in plants and fungi are crucial for the success of this technology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)