Review
Cell Biology
Giuseppa D'Amico, Radha Santonocito, Alessandra Maria Vitale, Federica Scalia, Antonella Marino Gammazza, Claudia Campanella, Fabio Bucchieri, Francesco Cappello, Celeste Caruso Bavisotto
Summary: Air pollution has become a growing problem, negatively impacting society and causing various health-related issues. Despite knowing the types and levels of pollutants, the molecular mechanisms by which they affect the human body are not fully understood. Recent evidence suggests that non-coding RNAs carried by extracellular vesicles may play a crucial role in regulating gene expression in response to pollution-induced multiorgan disorders. This review highlights the involvement of extracellular vesicle-transported non-coding RNAs in physiological and pathological conditions, including cancer, respiratory diseases, neurodegenerative diseases, and cardiovascular diseases caused by environmental stressors.
Review
Cell Biology
Milena Zarkovic, Franziska Hufsky, Udo R. Markert, Manja Marz
Summary: Non-coding RNAs have a central and regulatory role in the placenta, especially microRNAs. Syncytiotrophoblast cells act as a communication interface between the fetus and the mother through the release of extracellular vesicles containing ncRNAs. Disorders of ncRNAs in placental tissue are associated with pregnancy disorders and have potential as diagnostic markers.
Review
Oncology
Gu Yang, Yanjiao Wu, Rongxue Wan, Hongxun Sang, Huan Liu, Wenhua Huang
Summary: Osteosarcoma is an aggressive bone tumor with early metastasis potential and poor response to chemoradiotherapy. Non-coding RNAs have been shown to play a significant role in the initiation and progression of this tumor.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2021)
Review
Pathology
Ketao Li, Liping Ma, Zhiwei Lu, Laixing Yan, Wan Chen, Bing Wang, Huiju Xu, Zatollah Asemi
Summary: Heart failure is a cardiovascular condition where the heart fails to pump blood and oxygen adequately. Apoptosis, a controlled cell death process, affects various cardiovascular illnesses, including myocardial infarction and reperfusion injury. Non-coding RNAs (ncRNAs) have been found to influence protein stability, transcription factor control, and apoptosis in heart failure. Exosomes play a significant role in disease regulation and organ communication, but it is unclear if they modulate the interaction between cardiomyocytes and tumor cells in ischemic heart failure. This article highlights the ncRNAs associated with apoptosis in heart failure and emphasizes the importance of exosomal ncRNAs in the disease.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Review
Cell Biology
Nasrin Ahangar Davoodi, Sajad Najafi, Zari Naderi Ghale-Noie, Ashkan Piranviseh, Samaneh Mollazadeh, Sahar Ahmadi Asouri, Zatollah Asemi, Mohammadamin Morshedi, Seyed Saeed Tamehri Zadeh, Michael R. Hamblin, Amirhossein Sheida, Hamed Mirzaei
Summary: Retinoblastoma (RB) is a rare aggressive intraocular malignancy of childhood that can have severe consequences. Non-coding RNAs (ncRNAs) play important roles in the pathogenesis and development of RB, with different ncRNAs acting as either tumor promoters or suppressors. Understanding the regulatory mechanisms of ncRNAs provides new opportunities for RB therapy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Medicine, General & Internal
Yachen Shi, Qingyun Wang, Ruize Song, Yan Kong, Zhijun Zhang
Summary: Non-coding RNAs, such as microRNAs, circular RNAs, and long non-coding RNAs, play important roles in regulating normal biological processes and may be implicated in the pathogenesis of human diseases like depression. Clinical studies have shown that abnormal expression of ncRNAs in depressed patients compared to healthy individuals, with potential for ncRNAs to serve as biomarkers for diagnosis and treatment. Despite uncertainties regarding their precise roles, ncRNAs demonstrate promising clinical value in the management of depression.
Review
Oncology
Amirhosein Maharati, Yalda Samsami, Hanieh Latifi, Faezeh Tolue Ghasaban, Meysam Moghbeli
Summary: Chemotherapy is commonly used as a first-line therapy for cancer patients, but drug resistance poses a significant challenge. Long non-coding RNAs (lncRNAs) have been found to play a crucial role in regulating cellular processes associated with drug resistance. This review discusses the molecular mechanisms by which lncRNAs regulate the response to the nucleoside analog gemcitabine (GEM) in cancer patients. LncRNAs are reported to have an oncogenic role in inducing GEM resistance through various pathways, suggesting their potential as non-invasive markers to predict GEM response in patients.
CANCER CELL INTERNATIONAL
(2023)
Review
Medicine, Research & Experimental
Reza Rikhtegar, Pascal J. Mosimann, Jan Rothaupt, Mohammad Mirza-Aghazadeh-Attari, Shahin Hallaj, Mehdi Yousefi, Atefeh Amiri, Ebrahim Farashi, Atefeh Kheyrollahiyan, Sanam Dolati
Summary: Non-coding RNAs play critical roles in modulating the pathogenesis of IA, involving vascular development, growth, remodeling, and inflammation processes.
Review
Biochemistry & Molecular Biology
Irina Bure, Marina Nemtsova, Ekaterina B. Kuznetsova
Summary: In recent years, scientists have increasingly suggested and confirmed that epigenetic regulators are closely linked and form a comprehensive network of regulatory pathways and feedback loops. This is particularly important for a better understanding of the development and progression of various diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Cinzia Aurilia, Simone Donati, Gaia Palmini, Francesca Miglietta, Irene Falsetti, Teresa Iantomasi, Maria Luisa Brandi
Summary: Tumors of the parathyroid glands are common endocrine diseases characterized by parathyroid hormone hypersecretion, with a wide range of clinical presentations and typically treated through surgical removal. Recent studies have suggested that alterations to the epigenetic mechanisms could play a pivotal role in tumorigenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Jia Wu, Ying Zhu, Qingwei Cong, Qiumin Xu
Summary: Autophagy is a process that supports nutrient cycling and metabolic adaptation through lysosomal degradation. Recent studies have found that miRNAs and lncRNAs play important roles in the occurrence, invasion, development, and drug resistance of hepatocellular carcinoma (HCC) by regulating HCC cell autophagy. Understanding the specific miRNAs and lncRNAs involved and the related molecular mechanisms is crucial for HCC therapeutics.
Article
Biochemistry & Molecular Biology
Hitesh Kore, Keshava K. Datta, Shivashankar H. Nagaraj, Harsha Gowda
Summary: Recent studies have discovered many proteins encoded by noncoding regions in the human genome. These proteins are often encoded by small open reading frames in the UTRs of mRNAs and lncRNAs, and show poor evolutionary conservation. Some of them have been functionally characterized and play important roles in fundamental biological processes and human diseases. The number of novel protein coding regions in the human genome and their functional importance remain unknown.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Review
Medicine, Research & Experimental
Yuchan Yuan, Ling Xu, Zhaohong Geng, Jingjing Liu, Lijiao Zhang, Yuhang Wu, Dan He, Peng Qu
Summary: Atherosclerosis is the primary culprit of cardiovascular and cerebrovascular diseases, and non-coding RNA plays a regulatory role in the development and progression of atherosclerosis.
Review
Pharmacology & Pharmacy
Soudeh Ghafouri-Fard, Mohammad Taheri
Summary: Recent studies have shown that non-coding transcripts, including miRNAs and lncRNAs, play important roles in the pathobiology of obesity. Some lncRNAs are found to be down-regulated in obese patients, while others are up-regulated. Additionally, many miRNAs show differential expression between obese and non-obese subjects, as well as between mature adipocytes and pre-adipocytes.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Review
Cell Biology
Hang Li, Qiyue Zheng, Xinyan Xie, Jiaojiao Wang, Haihong Zhu, Haoye Hu, Hao He, Qiong Lu
Summary: Exosomal non-coding RNAs play a crucial role in the treatment of bone-related diseases by regulating the development of such diseases through intercellular communication among various cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Veronica Miguel, Jessica Tituana, J. Ignacio Herrero, Laura Herrero, Dolors Serra, Paula Cuevas, Coral Barbas, Diego Rodriguez Puyol, Laura Marquez-Exposito, Marta Ruiz-Ortega, Carolina Castillo, Xin Sheng, Katalin Susztak, Miguel Ruiz-Canela, Jordi Salas-Salvado, Miguel A. Martinez Gonzalez, Sagrario Ortega, Ricardo Ramos, Santiago Lamas
Summary: The study found that promoting fatty acid oxidation to increase mitochondrial oxidative metabolism can protect against kidney fibrosis, as demonstrated in animal models and patients with chronic kidney disease.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Biochemistry & Molecular Biology
Veronica Miguel, Ricardo Ramos, Laura Garcia-Bermejo, Diego Rodriguez-Puyol, Santiago Lamas
Summary: Excessive accumulation of extracellular matrix is a hallmark of fibrotic diseases, and miR-150 and miR-495 may contribute to renal fibrogenesis by aggravating the metabolic failure critically involved in tubular epithelial cells.
Article
Cardiac & Cardiovascular Systems
Taijyu Satoh, Longfei Wang, Cristina Espinosa-Diez, Bing Wang, Scott A. Hahn, Kentaro Noda, Elizabeth R. Rochon, Matthew R. Dent, Andrea R. Levine, Jeffrey J. Baust, Samuel Wyman, Yijen L. Wu, Georgios A. Triantafyllou, Ying Tang, Mike Reynolds, Sruti Shiva, Cynthia St Hilaire, Delphine Gomez, Dmitry A. Goncharov, Elena A. Goncharova, Stephen Y. Chan, Adam C. Straub, Yen-Chun Lai, Charles F. McTiernan, Mark T. Gladwin
Summary: This study elucidated the mechanisms through which metabolic syndrome contributes to pulmonary vascular dysfunction and exercise-induced pulmonary hypertension in patients with heart failure with preserved ejection fraction. Restoring NFYA-sGC beta 1-cGMP signaling can ameliorate EIPH.
Article
Cell Biology
Namita Chatterjee, Eugenia Fraile-Bethencourt, Adrian Baris, Cristina Espinosa-Diez, Sudarshan Anand
Summary: The study demonstrates the functional role of miR-494 in ER stress, showing that its expression is affected by ER stress inducers and hyperglycemia. Pre-treatment with miR-494 can mitigate ER stress induced by ER stress inducers and increase cell viability. It is suggested that ER stress-driven miR-494 may function in a feedback inhibitory loop.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Francesc X. Guix, Ana Marrero Capitan, Alvaro Casadome-Perales, Irene Palomares-Perez, Ines Lopez del Castillo, Veronica Miguel, Leigh Goedeke, Mauricio G. Martin, Santiago Lamas, Hector Peinado, Carlos Fernandez-Hernando, Carlos G. Dotti
Summary: As neurons age, their ability to degrade proteins and membranes decreases, leading to reduced cell function and survival. Aging neurons in culture secrete more small extracellular vesicles filled with cholesterol, which may negatively impact neighboring and distant cells.
LIFE SCIENCE ALLIANCE
(2021)
Editorial Material
Urology & Nephrology
Veronica Miguel, Santiago Lamas
Summary: The metabolic impairment of kidney tubular cells plays a key role in the pathophysiology of renal fibrosis, with a drastic reduction in fatty acid oxidation being a major factor contributing to global energy failure in the tubulointerstitial compartment. Additionally, the decreased expression of Krappel-like factor 15 in proximal tubular cells after kidney injury may further disrupt fatty acid oxidation.
KIDNEY INTERNATIONAL
(2021)
Review
Biochemistry & Molecular Biology
Miguel Fontecha-Barriuso, Ana M. Lopez-Diaz, Juan Guerrero-Mauvecin, Veronica Miguel, Adrian M. Ramos, Maria D. Sanchez-Nino, Marta Ruiz-Ortega, Alberto Ortiz, Ana B. Sanz
Summary: Acute kidney injury (AKI) and chronic kidney disease (CKD) are interconnected conditions, and mitochondrial dysfunction and oxidative stress play important roles in the progression of both diseases. New therapeutic approaches targeting mitochondria are needed, but there are barriers to clinical development, such as the lack of diagnostic tests for renal mitochondrial dysfunction.
Article
Oncology
Xiaomin Ding, Yawen Zhang, Jinrong Liang, Junyi Yin, Naveed Akbar, Veronica Miguel, Yan Zhou
Summary: The long-noncoding RNA colorectal neoplasia differentially expressed (CRNDE) gene is upregulated in osteosarcoma (OS) and is associated with clinical stage, lung metastasis, and poor prognosis. Overexpression of CRNDE promotes cell proliferation and migration in OS. CRNDE acts as a competitive endogenous RNA (ceRNA) to inhibit miR-136-5p, which further targets MRP9. Thus, CRNDE may serve as a significant marker for OS prognosis.
ANNALS OF TRANSLATIONAL MEDICINE
(2022)
Article
Immunology
Stefanie K. Wculek, Ignacio Heras-Murillo, Annalaura Mastrangelo, Diego Mananes, Miguel Galan, Veronica Miguel, Andrea Curtabbi, Coral Barbas, Navdeep S. Chandel, Jose Antonio Enriquez, Santiago Lamas, David Sancho
Summary: This study identifies oxidative phosphorylation (OXPHOS) as the most important process in distinguishing tissue macrophage populations in different organs. Impairing OXPHOS has differential effects on tissue macrophages, leading to reduced alveolar macrophages and selectively depleting pro-inflammatory white adipose tissue macrophages. OXPHOS is critical for maintaining tissue macrophages with high lipid-handling activity, making it a potential therapeutic target.
Review
Biochemistry & Molecular Biology
Veronica Miguel, Rafael Kramann
Summary: Fibrosis caused by excessive accumulation of ECM is a characteristic of CKD. Myofibroblasts, responsible for ECM production, are activated by communication with injured proximal tubule and immune cells. Recent evidence shows that metabolic alterations play a significant role in renal fibrosis pathogenesis. Using omics technologies and functional data, specific metabolic shifts in different cell types have been found to play specific roles in the fibrogenic response. This review summarizes the main outcomes of metabolic reprogramming in renal fibrosis and provides a future perspective on renal fibrometabolism.
Meeting Abstract
Biochemistry & Molecular Biology
Veronica Miguel, Carlos Rey-Serra, Jessica Tituana, Belen Sirera, J. Ignacio Herrero, Irene Ranz, Laura Fernandez, Carolina Castillo, Lucia Sevilla, James Nagai, Katharina C. Reimer, Jitske Jansen, Rafael Kramann, Ivan G. Costa, Ana Castro, Jose Miguel Rodriguez Gonzalez-Moro, Santiago Lamas
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Paula Cuevas-Delgado, Veronica Miguel, Francisco J. Ruperez, Santiago Lamas, Coral Barbas
Summary: Chronic kidney disease (CKD) is characterized by progressive and irreversible deterioration of kidney function and structure. The study investigated the renal metabolome in a mouse model with enhanced fatty acid oxidation (FAO) subjected to folic acid nephropathy (FAN) using a multiplatform untargeted metabolomics approach. The results identified significant metabolic changes associated with fibrosis and highlighted the importance of studying the relationship between metabolism and fibrosis in CKD progression.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biochemical Research Methods
Veronica Miguel, Katharina Charlotte Reimer, Anna Katharina Galyga, Jitske Jansen, Julia Moellmann, Luisa Meyer, Rebekka K. Schneider, Rafael Kramann
Summary: Metabolic derangement plays a crucial role in kidney pathophysiology, and organoids have shown great promise as an in vitro tool for kidney research. In this study, we present a refined protocol for analyzing bioenergetics in single kidney organoids derived from human induced pluripotent stem cells (iPSCs) using Seahorse XF96. Our protocol includes generating self-organized three-dimensional kidney organoids and preparing them for Seahorse XF96 analysis, followed by stress tests to determine mitochondrial and glycolytic rates in the organoids.
Article
Biochemistry & Molecular Biology
Veronica Miguel, Carlos Rey-Serra, Jessica Tituana, Belen Sirera, Elena Alcalde-Estevez, J. Ignacio Herrero, Irene Ranz, Laura Fernandez, Carolina Castillo, Lucia Sevilla, James Nagai, Katharina C. Reimer, Jitske Jansen, Rafael Kramann, Ivan G. Costa, Ana Castro, David Sanchoj, Jose Miguel Rodriguez Gonzalez-Moro, Santiago Lamas
Summary: In COVID-19-induced lung and renal damage, promoting cellular energy production through enhanced fatty acid oxidation (FAO) may be beneficial. The drugs metformin and baicalin, which activate AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) signaling, were shown to improve lung and kidney damage and reduce inflammation and fibrosis.
Article
Biochemistry & Molecular Biology
Sofia Campillo, Lourdes Bohorquez, Elena Gutierrez-Calabres, Diego Garcia-Ayuso, Veronica Miguel, Mercedes Griera, Yolanda Calle, Sergio de Frutos, Manuel Rodriguez-Puyol, Diego Rodriguez-Puyol, Laura Calleros
Summary: Chronic kidney disease can lead to cardiovascular damage, and a study has identified an enzyme called integrin-linked kinase (ILK) that plays a role in this process. ILK stimulates the formation of protrusions in white blood cells, causing them to become more mobile and potentially causing inflammation-mediated injury. Inactivating ILK can reverse these effects, suggesting that targeting this enzyme could help reduce cardiovascular complications associated with chronic kidney disease.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2022)
