Article
Multidisciplinary Sciences
Yuying Tan, Haonan Lin, Ji-Xin Cheng
Summary: This study demonstrates a high-content hyperspectral stimulated Raman scattering (h(2)SRS) imaging approach that allows for simultaneous mapping of multiple biomolecules at the single-cell level, revealing chemotherapy-induced metabolic reprogramming and unique metabolic features of chemoresistance. This approach could aid in the discovery of therapeutic targets to combat chemoresistance.
Article
Medicine, Research & Experimental
Dorinne Desposito, Ina Maria Schiessl, Georgina Gyarmati, Anne Riquier-Brison, Audrey K. Izuhara, Hiroyuki Kadoya, Balint Der, Urvi Nikhil Shroff, Young-Kwon Hong, Janos Peti-Peterdi
Summary: Utilizing multiphoton microscopy of transgenic Cdh5-Confetti mice, researchers were able to track and study the remodeling and repair of endothelial cells in the kidney and other organs, providing insights into the mechanisms of endothelial cells under physiological and disease conditions.
Article
Chemistry, Analytical
Zhaoyan Tian, Yafeng Wu, Fengying Shao, Dezhi Tang, Xiang Qin, Chenchen Wang, Songqin Liu
Summary: An integrated microfluidic system was designed for real-time monitoring of glycan expression on the surface of single tumor cells, achieving a level of 5.0 x 10^8 molecules per cell. This platform has the capability to distinguish single cancer cells from normal cells based on the expression level of glycans, providing a simple and accessible method for single-cell analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Engineering, Biomedical
Hequn Zhang, Peng Fu, Yin Liu, Zheng Zheng, Liang Zhu, Mengqi Wang, Marwan Abdellah, Mubin He, Jun Qian, Anna Wang Roe, Wang Xi
Summary: This study introduces a bright AIE probe and a lab-modified three-photon fluorescence microscopy system, achieving large-depth vascular imaging in the macaque monkey cortex and performing functional measurement and comparison across species. It is the first in vivo three-photon fluorescence microscopic imaging research on the macaque monkey cortex, providing a new method for understanding vascular function in humans in normal and disease conditions.
Article
Biochemistry & Molecular Biology
Sangram Nag, Mahabuba Jahan, Miklos Toth, Ryuji Nakao, Andrea Varrone, Christer Halldin
Summary: The study found that [F-18]FE-DTBZ-d4 has higher brain uptake and faster washout rate in nonhuman primate brains, showing better imaging properties and higher binding potential values compared to [C-11]DTBZ and [F-18]FE-DTBZ. Additionally, [F-18]FE-DTBZ-d4 exhibited faster radiometabolism in plasma, indicating increased stability against defluorination.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Baosheng Chen, Devi Prasan Ojha, Takuya Toyonaga, Jie Tong, Richard Pracitto, Monique A. Thomas, Michael Liu, Michael Kapinos, Li Zhang, Ming-Qiang Zheng, Daniel Holden, Krista Fowles, Jim Ropchan, Nabeel Nabulsi, Henk De Feyter, Richard E. Carson, Yiyun Huang, Zhengxin Cai
Summary: Currently, there are multiple clinical trials using PARP inhibitors in the treatment of glioblastoma. This study reports the synthesis of a brain-penetrant PARP PET tracer and its evaluations in a rat glioblastoma model and healthy nonhuman primates. The tracer demonstrated specific binding to PARP and showed potential for noninvasive quantification of PARP in the brain.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2023)
Article
Multidisciplinary Sciences
Alexandra N. Rindone, Xiaonan Liu, Stephanie Farhat, Alexander Perdomo-Pantoja, Timothy F. Witham, Daniel L. Coutu, Mei Wan, Warren L. Grayson
Summary: Vascularization plays a critical role in cranial bone growth, maintenance, and healing. The study utilized a quantitative imaging platform to analyze the spatial relationship between blood vessels and skeletal progenitors in the murine calvarium, demonstrating specific vessel types are spatially correlated with skeletal progenitors during various cranial processes. Furthermore, the findings suggest a potential role for osteoclasts in maintaining the native cranial microvascular environment.
NATURE COMMUNICATIONS
(2021)
Article
Biology
Alyssa C. C. Fears, Edith M. M. Walker, Nicole Chirichella, Nadia Slisarenko, Kristen M. M. Merino, Nadia Golden, Breanna Picou, Skye Spencer, Kasi E. E. Russell-Lodrigue, Lara A. A. Doyle-Meyers, Robert V. V. Blair, Brandon J. J. Beddingfield, Nicholas J. J. Maness, Chad J. J. Roy, Namita Rout
Summary: This study shows that peripheral gamma delta T cells are rapidly activated following SARS-CoV-2 infection in nonhuman primates. The expansion of V delta 1 gamma delta T cells at day 1 is significantly correlated with lung viral loads during the first week of infection. The increase in CCR6 and Granzyme B expression in V delta 1 T cells implies a role in innate-like epithelial barrier-protective and cytotoxic functions during viral clearance. Additionally, the early activation and mobilization of circulating HLA-DR(+)CXCR3(+) gamma delta T cells, along with correlations with IL-1Ra and SCF levels in bronchoalveolar lavage, suggest a novel role for V delta 1 T cells in regulating lung inflammation during aerosol SARS-CoV-2 infection.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Moeko Sato, Hiroko Akashi, Yuki Sakamoto, Sachihiro Matsunaga, Hiroyuki Tsuji
Summary: This study established a protocol for tissue clearing and 3D imaging in rice, allowing for cellular-resolution 3D imaging of rice shoot apical meristems, florets, and root apical meristems. Using fluorescent reporters, the 3D distribution of auxin signaling events in rice root tips was revealed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Kyle L. O'Donnell, Friederike Feldmann, Benjamin Kaza, Chad S. Clancy, Patrick W. Hanley, Paige Fletcher, Andrea Marzi
Summary: Marburg virus (MARV) is a highly pathogenic agent with no available treatments or vaccines. This study demonstrates that a vesicular stomatitis virus (VSV)-based vaccine expressing the MARV-Angola glycoprotein has a potential as a viable and fast-acting MARV vaccine candidate, providing uniform protection against the virus when administered in lower doses.
Article
Biochemistry & Molecular Biology
Chi-Hyeon Yoo, Zhen Chen, Nisha Rani, Jiahui Chen, Jian Rong, Laigao Chen, Lei Zhang, Steven H. Liang, Hsiao-Ying Wey
Summary: This study evaluated the methods of PET quantification for [18F]PF-06455943, a radioligand for LRRK2 imaging. The results showed that a two-tissue compartmental model and a Logan graphical analysis were appropriate for PET quantification. Additionally, it was found that total distribution volume (VT) values could be reliably estimated with as short as a 30 min scan duration. Homologous blocking studies confirmed the specific binding of [18F]PF-06455943 and revealed that the nonradioactive mass of PF-06455943 achieved 45-55% of VT displacement in the whole brain. This work supports the translation of [18F]PF-06455943 PET imaging for the human brain and target occupancy studies.
ACS CHEMICAL NEUROSCIENCE
(2023)
Article
Multidisciplinary Sciences
Esley Torres-Garcia, Raul Pinto-Camara, Alejandro Linares, Damian Martinez, Victor Abonza, Eduardo Brito-Alarcon, Carlos Calcines-Cruz, Gustavo Valdes-Galindo, David Torres, Martina Jablonski, Hector H. Torres-Martinez, Jose L. Martinez, Haydee O. Hernandez, Jose P. Ocelotl-Oviedo, Yasel Garces, Marco Barchi, Rocco D'Antuono, Ana Boskovic, Joseph G. Dubrovsky, Alberto Darszon, Mariano G. Buffone, Roberto Rodriguez Morales, Juan Manuel Rendon-Mancha, Christopher D. Wood, Armando Hernandez-Garcia, Diego Krapf, Alvaro H. Crevenna, Adan Guerrero
Summary: This study presents a new super-resolution microscopy algorithm called Mean-Shift Super Resolution (MSSR), which extends the spatial resolution of single fluorescence images beyond the diffraction limit. MSSR works on various fluorophore densities and optical setups, and has denoising capabilities.
NATURE COMMUNICATIONS
(2022)
Article
Engineering, Mechanical
Chi-Hyeon Yoo, Zhen Chen, Nisha Rani, Jiahui Chen, Jian Rong, Laigao Chen, Lei Zhang, Steven H. Liang, Hsiao-Ying Wey
Summary: Mutations in the LRRK2 gene are common causes of inherited Parkinson's disease. A novel PET radioligand, [F-18]PF-06455943, has been developed for LRRK2 imaging. The quantification methods for [F-18]PF-06455943 PET imaging in nonhuman primate brains have been evaluated, and the specific binding of the radioligand has been confirmed.
JOURNAL OF FLUIDS AND STRUCTURES
(2023)
Review
Chemistry, Analytical
Yucheng Sun, Zengnan Wu, Yuting Shang, Seong Ho Kang, Jin-Ming Lin
Summary: This article provides a brief overview of the technical development, applications, challenges, and future directions in single-cell analysis using single-molecule detection-based super-resolution microscopy (SMDSRM), aiming to guide researchers in promoting the methodological development and exploring the potential of SMDSRM for single-cell analysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Neurosciences
Jason F. Moody, Nakul Aggarwal, Douglas C. Dean, Do P. M. Tromp, Steve R. Kecskemeti, Jonathan A. Oler, Ned H. Kalin, Andrew L. Alexander
Summary: Alterations in white matter development are related to neuropsychiatric disorders. Quantitative relaxometry can be used to evaluate white matter microstructural changes. A study on infant non-human primates found correlations between quantitative relaxometry and diffusion tensor imaging metrics, with gestational age being a stronger predictor for relaxometry metrics. This provides a basis for future studies.
Article
Optics
A. Guevara-Torres, D. R. Williams, J. B. Schallek
Article
Multidisciplinary Sciences
Juliette E. McGregor, Tyler Godat, Kamal R. Dhakal, Keith Parkins, Jennifer M. Strazzeri, Brittany A. Bateman, William S. Fischer, David R. Williams, William H. Merigan
NATURE COMMUNICATIONS
(2020)
Article
Ophthalmology
Kamal R. Dhakal, Sarah Walters, Juliette E. McGregor, Christina Schwarz, Jennifer M. Strazzeri, Ebrahim Aboualizadeh, Brittany Bateman, Krystel R. Huxlin, Jennifer J. Hunter, David R. Williams, William H. Merigan
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2020)
Article
Multidisciplinary Sciences
In-Kyu Lee, Allison L. Ludwig, M. Joseph Phillips, Juhwan Lee, Ruosen Xie, Benjamin S. Sajdak, Lindsey D. Jager, Shaoqin Gong, David M. Gamm, Zhenqiang Ma
Summary: Polymeric scaffolds are being used in therapeutics for blinding disorders affecting the outer retina, with recent advances in engineering producing optimized structures for retinal pigment epithelium delivery. A new biodegradable scaffold patterned for high-density photoreceptor replacement has been developed, which may have applications in multicellular layers for outer retinal reconstruction and other tissues. This approach could also be useful for producing various micro- and nanoscale structures for multilayered cell delivery.
Article
Biotechnology & Applied Microbiology
Juliette E. McGregor, Karteek Kunala, Zhengyang Xu, Peter J. Murphy, Tyler Godat, Jennifer M. Strazzeri, Brittany A. Bateman, William S. Fischer, Keith Parkins, Colin J. Chu, Teresa Puthussery, David R. Williams, William H. Merigan
Summary: This study examined the long-term changes in optogenetic responsivity of foveal retinal ganglion cells (RGCs) in non-human primates following localized photoreceptor ablation. The results showed that RGCs maintained good cellular and structural preservation and continued to exhibit optogenetic responses for at least one year after the loss of photoreceptor input.
Review
Biochemistry & Molecular Biology
Kelsy L. Nilles, Erica I. Williams, Robert D. Betterton, Thomas P. Davis, Patrick T. Ronaldson
Summary: Stroke is a major cause of death and disability worldwide. Despite successful identification of neuroprotective agents in preclinical stroke studies, none of these drugs have advanced beyond phase III clinical trials. One reason for these failures is the lack of consideration of blood-brain barrier (BBB) transport mechanisms. This review discusses specific BBB uptake transporters that can be targeted to improve neuroprotective drug delivery and presents perspectives on integrating transporter pharmacology into preclinical stroke research. Considerations such as species selection and co-morbid conditions are also discussed to optimize translational success.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Ophthalmology
Allison L. Ludwig, David M. Gamm
Summary: Retinal degenerative diseases affecting photoreceptors are a major cause of incurable blindness, and the use of human pluripotent stem cell technologies for retinal cell replacement therapies has shown progress but also presents numerous challenges and unanswered questions.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2021)
Article
Cell Biology
Sarah K. Rempel, Madalynn J. Welch, Allison L. Ludwig, M. Joseph Phillips, Yochana Kancherla, Donald J. Zack, David M. Gamm, Timothy M. Gomez
Summary: Early-born photoreceptors exhibit autonomous axon extension from dynamic terminals, while late-born photoreceptors lose dynamic terminals but can still extend axons by attaching to non-photoreceptor cells.
Article
Cell & Tissue Engineering
Ana Ripolles-Garcia, Natalia Dolgova, M. Joseph Phillips, Svetlana Savina, Allison L. Ludwig, Sara A. Stuedemann, Uchenna Nlebedum, John H. Wolfe, Oliver A. Garden, Arvydas Maminishkis, Juan Amaral, Kapil Bharti, David M. Gamm, Gustavo D. Aguirre, William A. Beltran
Summary: Researchers have successfully transplanted human photoreceptor precursor cells into dogs with inherited retinal degeneration, showing potential for treating this condition.
Article
Veterinary Sciences
Rachel Taber, Anne Pankowski, Allison L. Ludwig, Maggie Jensen, Veronica Magsamen, Erin Lashnits
VETERINARY CLINICS OF NORTH AMERICA-SMALL ANIMAL PRACTICE
(2022)
Article
Cell & Tissue Engineering
Steven J. Mayerl, Simona Bajgai, Allison L. Ludwig, Lindsey D. Jager, Brittany N. Williams, Cole Bacig, Christopher Stoddard, Divya Sinha, Benjamin D. Philpot, David M. Gamm
Summary: This study found that IMPG2 mutations cause early-onset retinitis pigmentosa and identified the mechanism behind it by demonstrating the loss of outer segment layer in patient-derived retinal organoids. Transplantation of mutant organoids into protected spaces restored outer segment production. This model system provides a robust platform for studying IMPG2-RP and may have implications for treating other photoreceptor-based diseases.
Article
Multidisciplinary Sciences
Allison L. Ludwig, Steven J. Mayerl, Yu Gao, Mark Banghart, Cole Bacig, Maria A. Fernandez Zepeda, Xinyu Zhao, David M. Gamm
Summary: This study demonstrates the ability of human pluripotent stem cell-derived retinal organoids to establish new synaptic connections after dissociation, enhancing their potential for use in cell replacement strategies. A high-throughput approach for labeling and quantifying traced retinal cell types was developed, with photoreceptors and retinal ganglion cells being the major contributing populations. This system provides a platform for assessing and enhancing synaptogenesis in cultured retinal neurons.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Ophthalmology
Hongxin Song, Ethan A. Rossi, David R. Williams
Summary: Idiopathic macular telangiectasia (MacTel) is primarily considered a vascular disease affecting juxtafoveal retinal capillaries, but recent evidence suggests that neuronal changes may occur early. Using high-resolution adaptive optics retinal imaging, this study found that foveal cone density is lower in the clinically less-affected eyes of patients with asymmetric MacTel, indicating that cone photoreceptor loss may precede classic vascular changes in MacTel.
BMJ OPEN OPHTHALMOLOGY
(2021)
