Review
Cell Biology
Julio C. C. Fierro Morales, Qian Xue, Minna Roh-Johnson
Summary: Cell-substrate adhesion is crucial for cell migration, with focal adhesions being a well-studied structure involved in this process. However, the understanding of the evolution and in vivo role of cell-substrate adhesion machinery is limited. Further investigation in these areas is important for understanding the role of cell-substrate adhesion in physiological contexts and for potential applications in adhesion-based processes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Oindrila De, Clinton Rice, Teresa Zulueta-Coarasa, Rodrigo Fernandez-Gonzalez, Robert E. Ward
Summary: In Drosophila, mutations in core SJ proteins not only affect the occluding function of SJs, but also disrupt developmental events such as cell shape changes and adhesion in the process of dorsal closure. These mutations result in defects in DC and reduced levels of E-cadherin.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Pasquale Sacco, Francesco Piazza, Chiara Pizzolitto, Gabriele Baj, Francesco Brun, Eleonora Marsich, Ivan Donati
Summary: This study presents a strategy to control the dissipation rate of viscoplastic substrates while precisely regulating linear elasticity, and reveals the crucial link between this material property and cellular responses in 2D cultures.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Engineering, Manufacturing
Ziting Tan, Hongwei Li, Yinan Huang, Xue Gong, Jiannan Qi, Jie Li, Xiaosong Chen, Deyang Ji, Weibang Lv, Liqiang Li, Wenping Hu
Summary: By optimizing the surface topography of PEDOT:PSS/PDMS bimorph pressure sensors with the BEAT strategy, fast response time, high sensitivity, excellent repeatability, and stability can be achieved. Transferring other conductive polymers or metal nanowires onto PDMS with robust adhesion further improves stability and expands the applications of flexible electronics.
COMPOSITES PART A-APPLIED SCIENCE AND MANUFACTURING
(2021)
Article
Nanoscience & Nanotechnology
Xuran Guo, Xianliu Wang, Han Tang, Yajuan Ren, Donghong Li, Bingcheng Yi, Yanzhong Zhang
Summary: This study demonstrates the use of constrained shape recovery stress to enhance stem cell tenodifferentiation. The shape memory effect (SME) exerted in situ mechanical stimulus on the cells, promoting cellular elongation and upregulating tendon-specific markers. The Rho/ROCK signaling pathway is involved in the enhancement of tenodifferentiation.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Cell Biology
Ineke D. C. Jansen, Thijs van Velzen, Teun J. de Vries, Robert Szulcek, Jack J. W. A. van Loon
Summary: In this study, an impedimetric bioassay was introduced for real-time monitoring of osteoclast resorption. The method involves culturing osteoclasts on a calcium phosphate substrate with electrodes and applying different chemical stimuli to measure their activity continuously. The results showed that inflammatory factors and bisphosphonates significantly affected osteoclastic activity. This method is of great importance for studying osteoclast function and diseases, as well as for high-throughput drug testing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Agata Nyga, Katarzyna Plak, Martin Kraeter, Marta Urbanska, Kyoohyun Kim, Jochen Guck, Buzz Baum
Summary: Animal cells undergo repeated changes in shape, such as rounding up and respreading during division. The process of cell rounding can also be observed in interphase cells, particularly when cancer cells switch from a mesenchymal to an ameboid mode of migration. However, the mechanism of interphase cell rounding remains unclear.
Article
Chemistry, Multidisciplinary
Yichao Zhang, Moon-Ki Choi, Greg Haugstad, Ellad B. Tadmor, David J. Flannigan
Summary: This study demonstrates the formation of highly symmetric and high-fidelity strain patterns in single-crystal 2H-MoS2 flakes captured on amorphous holey silicon nitride substrates. The results show that predictable patterns and 3D topography can be spontaneously induced on bare, holey substrates.
Article
Chemistry, Physical
Elina Bondareva, Yuri Dekhtyar, Vladislavs Gorosko, Hermanis Sorokins, Alexander Rapoport
Summary: The adhesion ability of cells is correlated with the statistical distribution of electrical potential on the substrate's surface, highlighting the importance of considering this behavior in surface processing for cell adhesion based on cell size.
Article
Biophysics
Josephine Solowiej-Wedderburn, Carina M. Dunlop
Summary: Tissue stiffness plays a crucial role in cell behavior, and the arrangement of adhesions between cells and substrate is also important in determining the effective substrate stiffness experienced by cells. Different adhesion patterns result in equivalent mean cell deformations but different gel stiffnesses. Energy considerations can explain the observed focal adhesion growth and elongation on stiff substrates, as well as the absence of focal adhesions on soft substrates. The simulations suggest that fewer and larger adhesions, randomly placed, are preferred and result in realistic cell shapes.
BIOPHYSICAL JOURNAL
(2022)
Article
Engineering, Biomedical
Hao Ming, ChenXu Tian, Nan He, Xin Zhao, Feng Luo, Zhen Li, Jiehua Li, Hong Tan, Qiang Fu
Summary: Mussel-inspired polyurethane coatings (PU-LDAs) with enhanced adhesion and biocompatibility are synthesized through surface coating modification. These coatings can be used for surface modification of implant materials, reducing immune inflammatory response and improving cell adhesion and growth.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2022)
Article
Biochemical Research Methods
Davis B. Zhou, Maria Castanos, Alexander Pinhas, Peter Gillette, Justin Migacz, Richard B. Rosen, Jeffrey Glassberg, Toco Y. P. Chui
Summary: This study introduces imaging metrics to capture dynamic vascular changes in sickle cell disease patients, revealing significant variations in capillary perfusion compared to controls. These measurements could be useful for monitoring disease state and evaluating treatment efficacy.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Chemistry, Analytical
Anna Ronja Dorothea Binder, Andrej-Nikolai Spiess, Michael W. Pfaffl
Summary: Measurement of cell surface coverage using electric cell-substrate impedance sensing (ECIS) is a common technique for assessing cell growth behavior. A large toolset of R scripts was developed to transform and quantify ECIS growth curves in different experimental settings, allowing extraction of quantitative data for analysis. The scripts were tested in real-life cell culture experiments and calibration setups, providing comparability and reliable quantification.
Article
Physics, Applied
Chenwei Qi, Juanjuan Zhang, Ana-Suncana Smith, Long Li
Summary: Through theoretical investigation, we found that during stretching adhesion interface, the crack first develops at the stiff edge of the adhesion area and then grows to the soft adhesion edge until complete detachment is achieved. We also examined the lifetime of the bond cluster, interface adhesion strength, and the effect of inter-bond distance. These findings provide a detailed mechanistic understanding of the adhesion interface responding to the mechanical heterogeneities of the substrate at the molecular bond level.
JOURNAL OF APPLIED PHYSICS
(2023)
Article
Biotechnology & Applied Microbiology
Suk-Min Hong, Ji-Young Yoon, Jae-Ryung Cha, Junyong Ahn, Nandin Mandakhbayar, Jeong Hui Park, Junseop Im, Gangshi Jin, Moon-Young Kim, Jonathan C. Knowles, Hae-Hyoung Lee, Jung-Hwan Lee, Hae-Won Kim
Summary: The study reports a novel polycaprolactone-based polyurethane copolymer with hyperelasticity, shape-memory, and ultra-cell adhesion properties, which is considered a potential tissue-regenerative biomaterial, especially for minimally invasive surgery.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Paulina Natalia Osuchowska, Przemyslaw Wachulak, Wiktoria Kasprzycka, Agata Nowak-Stepniowska, Maciej Wakula, Andrzej Bartnik, Henryk Fiedorowicz, Elzbieta Anna Trafny
Summary: Understanding cancer cell adhesion through SXCM can provide valuable insights into tumor progression and metastasis, making it a promising technique for investigating actin cytoskeleton adhesion and organization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Iga Jancewicz, Joanna Szarkowska, Ryszard Konopinski, Malgorzata Stachowiak, Monika Swiatek, Katarzyna Blachnio, Szymon Kubala, Paulina Oksinska, Pawel Cwiek, Natalia Rusetska, Agnieszka Tupalska, Natalia Zeber-Lubecka, Ewa Grabowska, Bianka Swiderska, Agata Malinowska, Michal Mikula, Beata Jagielska, Jan Walewski, Janusz A. Siedlecki, Tomasz J. Sarnowski, Sergiusz Markowicz, Elzbieta A. Sarnowska
Summary: Tumors induce inhibitory signals to prevent attacks from tumor-infiltrating lymphocytes (TILs), leading to dysfunction and apoptosis of effector T cells. Continuous stimulation causes TILs to become exhausted, unable to kill tumor cells and produce cytokines, resulting in immune escape of cancer cells. This study suggests a potential role for EZH2 inhibitors as immunomodulators in cancer treatment.
Review
Cell Biology
Ewa A. Grzybowska, Maciej Wakula
Summary: Protein binding to the coding sequences (CDS) of mRNAs is common and plays roles in regulating mRNA stability as well as translational efficiency. Global approaches and specific examples highlight a relatively less-known regulatory network controlling mRNA stability and translation.
Editorial Material
Biochemistry & Molecular Biology
Roberto Giambruno, Ewa A. Grzybowska, Nicolas L. Fawzi, Dorothee Dormann
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Mostafa Kianfar, Anna Balcerak, Mateusz Chmielarczyk, Leszek Tarnowski, Ewa A. Grzybowska
Summary: Although the process of entosis has been extensively studied, there is still controversy regarding its molecular mechanisms and clinical significance. Actomyosin contractility of the invading cell and autophagy induced in the outer cell have been identified as the driving force and degradation mechanism, respectively. However, the diversity of triggering factors and molecular pathways involved in entosis regulation make interpretations difficult, and the physiological role of entosis remains unclear.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lukasz Tomorowicz, Beata Zolnowska, Krzysztof Szafranski, Jaroslaw Chojnacki, Ryszard Konopinski, Ewa A. Grzybowska, Jaroslaw Slawniski, Anna Kawiak
Summary: In the search for new compounds with antitumor activity, new potential anticancer agents were designed as molecular hybrids containing the structures of a triazine ring and a sulfonamide fragment. The obtained compounds showed cytotoxic activity against tumor cell lines and induced cell cycle arrest and apoptosis. The study also revealed the key role of certain substituents for the cytotoxic activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Anna Balcerak, Ewelina Macech-Klicka, Maciej Wakula, Rafal Tomecki, Krzysztof Goryca, Malgorzata Rydzanicz, Mateusz Chmielarczyk, Malgorzata Szostakowska-Rodzos, Marta Wisniewska, Filip Lyczek, Aleksandra Helwak, David Tollervey, Grzegorz Kudla, Ewa A. Grzybowska
Summary: This study characterizes the RNA targets of HAX1 and reveals its involvement in translation, ribosome biogenesis, and rRNA processing. Knockouts of HAX1 show downregulation of transcripts partially overlapping with HAX1 RNA targets, suggesting a role in mRNA stabilization. The study also demonstrates a functional connection between HAX1 and ribosome biogenesis.
Article
Oncology
Beata Joanna Mossakowska, Natalia Rusetska, Ryszard Konopinski, Paulina Kober, Maria Maksymowicz, Monika Pekul, Grzegorz Zielinski, Andrzej Styk, Jacek Kunicki, Mateusz Bujko
Summary: This study investigates the role of deubiquitinase gene mutations in predicting the response to cell cycle inhibitors in patients with corticotroph pituitary tumors. The findings suggest that USP8 and USP48 mutations have significant effects on cell cycle-related genes and may influence the response to small molecule inhibitors. However, in vitro experiments show that overexpression or modulation of p27 expression does not affect treatment response.
Article
Biochemistry & Molecular Biology
Maciej Wakula, Anna Balcerak, Tymon Rubel, Mateusz Chmielarczyk, Ryszard Konopinski, Filip Lyczek, Ewa A. Grzybowska
BIOSCIENCE REPORTS
(2020)
Review
Biochemistry & Molecular Biology
Sylwia Tabor, Malgorzata Szostakowska-Rodzos, Anna Fabisiewicz, Ewa A. Grzybowska
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)