Article
Microbiology
Qun Lan, Yuju Lian, Peiya Peng, Long Yang, Heng Zhao, Peng Huang, Haiming Ma, Hongjiang Wei, Yulong Yin, Mei Liu
Summary: In this study, potential biomarkers and microbial species associated with finishing weight in Diannan small ear pigs were identified. Carbohydrate-decomposing bacteria were positively related to finishing weight, while microbial taxa associated with intestinal inflammation and damage had opposite effects. Gut microbial functional annotation analysis revealed that certain enzymes and SCFA levels, especially acetate and butyrate, played crucial roles in regulating finishing weight.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Jyoti Chhimwal, Prince Anand, Priyanka Mehta, Mohit Kumar Swarnkar, Vikram Patial, Rajesh Pandey, Yogendra Padwad
Summary: The importance of the gut-liver axis in the pathophysiology of metabolic dysfunction-associated fatty liver disease (MAFLD) is being investigated more closely in recent times. This study assesses the time-dependent gut dysbiosis in MAFLD, its implications in disease progression, and the role of plant-derived prebiotics in its attenuation.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Environmental Sciences
Li Zhang, Honghong Guo, Jie Gu, Ting Hu, Xiaojuan Wang, Yifan Sun, Huakang Li, Wei Sun, Xun Qian, Zilin Song, Jun Xie, Lu An
Summary: The gut microbiota serves as a reservoir for antibiotic resistance genes (ARGs), which can potentially impact the health of humans and animals. The impact of diet on the intestinal flora and antibiotic resistome in rabbits remains unclear. Feeding rabbits lettuce grown with different manure types leads to the amplification and transfer of exogenous ARGs in the intestinal flora, posing a potential threat to health.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Genetics & Heredity
Hiroshi Mori, Tamotsu Kato, Hiroaki Ozawa, Mitsuo Sakamoto, Takumi Murakami, Todd D. Taylor, Atsushi Toyoda, Moriya Ohkuma, Ken Kurokawa, Hiroshi Ohno
Summary: To understand the biases in human gut microbiome studies, a reference microbiome composed of an artificial mock community is crucial. However, there is a lack of suitable mock communities that accurately represent the human gut microbiome. In this study, we created a novel mock community consisting of 18 major bacterial species found in the human gut and examined the impact of experimental and bioinformatics procedures on 16S rRNA gene and shotgun metagenomic sequencing. Our findings revealed that DNA extraction methods significantly influenced DNA yields and taxonomic composition of sequenced reads and certain commonly used primers for 16S rRNA genes led to underestimation of certain gut commensal taxa. Additionally, we demonstrated that the binning of shotgun metagenomic sequences using MetaBAT2 produced consistent, less-contaminated bins with varying completeness. The use of multiple binning tools with MetaWRAP improved completeness but sometimes increased contamination rate. This benchmark study provides a crucial foundation for interpreting human gut microbiome data, allowing for standardization among different methodologies, and facilitating the advancement of analytical methods.
Article
Food Science & Technology
Marek Aljewicz, Beata Nalepa, Slawomir Ciesielski
Summary: This study investigated the impact of different β-glucans on the gut microbiome in rats and found that oat β-glucan and curdlan increased the abundance of specific bacterial genera, while a positive correlation was observed between Streptococcus bacteria and the growth rate of rats. Consuming β-glucans at the recommended dose by EFSA did not significantly alter the structure of the gut microbiome in rats.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Microbiology
Fei Jiang, Meiling Cai, Yanchun Peng, Sailan Li, Bing Liang, Hong Ni, Yanjuan Lin
Summary: This study investigates the differences in gut microbiota composition between patients with type A aortic dissection (AAD) and healthy controls. The microbiome composition can accurately predict AAD and is associated with inflammation. These findings will contribute to the development of preventive and therapeutic strategies for AAD.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Emily C. Fowler, Ryan S. Samuel, Benoit St-Pierre
Summary: For commercial swine producers, the natural variation in body weight amongst pigs in a herd presents a challenge in meeting the standards of meat processors who incentivize target carcass weights by offering more favorable purchase prices. The gut microbiome has emerged as an important factor that can affect growth performance and efficiency in swine, as it contributes to vital functions such as providing assimilable nutrients and resistance to infection. This study compared the fecal microbiomes of light and heavy barrows and identified two bacterial species in higher abundance in the light barrows group, which may play roles in promoting beneficial microorganisms and utilizing starch in the swine gut.
Article
Immunology
Xiaolin Liu, Nina van Beek, Aleksa Cepic, Nadia A. Andreani, Cecilia J. Chung, Britt M. Hermes, Kaan Yilmaz, Sandrine Benoit, Kossara Drenovska, Sascha Gerdes, Regine Glaeser, Matthias Goebeler, Claudia Guenther, Anabelle von Georg, Christoph M. Hammers, Maike M. Holtsche, Franziska Huebner, Dimitra Kiritsi, Franziska Schauer, Beke Linnenmann, Laura Huilaja, Kaisa Tasanen-Maatta, Snejina Vassileva, Detlef Zillikens, Christian D. Sadik, Enno Schmidt, Saleh Ibrahim, John F. Baines
Summary: Bullous pemphigoid patients have altered gut microbiota composition and function, with decreased microbial diversity and overall changed microbial community. Disease-associated gut microbial features, including reduced Faecalibacterium prausnitzii and increased pathways related to gamma-aminobutyric acid metabolism, are observed in BP patients. These findings suggest that gut microbiota alterations may play a role in the development of bullous pemphigoid.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Psychiatry
Xinyu Fang, Chunying Gao, Weifeng Wu, Xiuxiu Hu, Miaomiao Shao, Chou Zhou, Renliang Cai, Jin Fang, Yi Li, Yue Xu, Xiangrong Zhang
Summary: This study explored the relationship between weight gain in schizophrenia patients with atypical antipsychotic treatment and gut microbiome. The results showed that there were no significant differences in alpha diversity between normal-weight and overweight schizophrenia patients treated with atypical antipsychotics. However, the beta diversity analysis revealed distinct clustering patterns for overweight and normal-weight patients. The composition of the gut microbiome also differed between the two groups. Function prediction identified several pathways that were significantly different between groups. Correlation analysis indicated associations between specific bacteria and BMI. Overall, these findings suggest that perturbations in the gut microbiome composition and functional pathways may contribute to weight gain in schizophrenia patients treated with atypical antipsychotics.
PSYCHIATRY RESEARCH
(2023)
Article
Ecology
N. A. Baeshen, M. A. Majeed, M. A. Alotaibi, M. H. Alnefai, A. A. Abulfaraj, N. N. Baeshen, A. Emradiri, M. N. Baeshen
Summary: The study characterized the gut microbiome in Usherhopper, Poekilocerus bufonius, revealing Proteobacteria as the most common phylum and Wolbachia as the dominant genus. The intricate interactions between the microbiome and the host play a significant role in the ecosystem, warranting further research.
APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH
(2022)
Article
Fisheries
Olga Burtseva, Anna Kublanovskaya, Tatiana Fedorenko, Elena Lobakova, Konstantin Chekanov
Summary: This study revealed the previously undescribed bacterial diversity in the White Sea fish gut microbiota using 16S rRNA metabarcoding, showing specific patterns of bacteria common in the water of this region.
Article
Biochemistry & Molecular Biology
Casey T. Finnicum, Zahraa Rahal, Maya Hassane, Warapen Treekitkarnmongkol, Ansam Sinjab, Rhiannon Morris, Yuejiang Liu, Elizabeth L. Tang, Sarah Viet, Jason L. Petersen, Philip L. Lorenzi, Lin Tan, Joseph Petrosino, Kristi L. Hoffman, Junya Fujimoto, Seyed Javad Moghaddam, Humam Kadara
Summary: Research has shown that exposure to tobacco carcinogens leads to significant changes in the gut and lung microbiomes, which are associated with the development of lung adenocarcinoma (LUAD) and immunotherapeutic response in human lung cancer patients. Loss of the bacterial growth inhibitor Lcn2 also results in widespread changes in the gut and lung microbiomes. These findings shed light on the role of microbial phenotypes in the development of tobacco-associated LUAD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Jiakuan Niu, Xiao Liu, Junying Xu, Fen Li, Jincan Wang, Xixi Zhang, Xu Yang, Lin Wang, Sen Ma, Defeng Li, Xiaoyan Zhu, Chengzhang Wang, Yinghua Shi, Yalei Cui
Summary: This study investigated the effects and mechanisms of mulberry and paper mulberry silages on growth performance, meat quality, and intestinal health of finishing pigs. The results showed that mulberry silage improved meat quality and increased the concentration of short-chain fatty acids (SCFAs). The microbial composition in the intestine was also affected by mulberry silage. These findings have important implications for improving pork quality.
MICROBIOLOGY SPECTRUM
(2023)
Article
Gastroenterology & Hepatology
Stefanie Brezina, Martin Borkovec, Andreas Baierl, Fabienne Bastian, Andreas Futschik, Nikolaus Gasche, Thomas Gruenberger, Michael Hallas, Christian Jannsen, Gernot Leeb, Rebecca Lutz, Barbara Sladek, Andrea Gsur
Summary: The study evaluated the usability of FIT cartridges for microbiome analysis and compared it to Stool Collection and Preservation Tubes. The FIT and Preservation Tube samples had highly similar microbiome profiles, with minor differences between abundances of some bacterial taxa. The results indicate that FIT cartridges are suitable for gut microbiome analysis within CRC screening programs.
Article
Gastroenterology & Hepatology
Zijie Jin, Andy Ng, Corinne F. Maurice, David Juncker
Summary: MiCoMo is a low-cost, benchtop multi-bioreactor system that simulates the human colon environment, allowing for the study of gut microbial communities with high throughput and multiplexing capabilities.
Article
Multidisciplinary Sciences
Xuan Chen, Chongde Lai, Yulan Wang, Lili Wei, Qiwang Zhong
Article
Biotechnology & Applied Microbiology
Shaoming Fang, Xuan Chen, Liwen Zhou, Chongchong Wang, Qiaohui Chen, Ruiyi Lin, Tianfang Xiao, QianFu Gan
MICROBIAL BIOTECHNOLOGY
(2019)
Article
Multidisciplinary Sciences
Xuan Chen, Shaoming Fang, Lili Wei, Qiwang Zhong
Article
Microbiology
Shaoming Fang, Xuan Chen, Jiahua Pan, Qiaohui Chen, Liwen Zhou, Chongchong Wang, Tianfang Xiao, Qian Fu Gan
Article
Microbiology
Xuan Chen, Lianghuan Wu, Huahui Lan, Ruilin Sun, Meifang Wen, Danrui Ruan, Mengjuan Zhang, Shihua Wang
Summary: The study revealed that MystA and MystB in Aspergillus flavus play significant roles in regulating conidiation, sclerotia formation, and aflatoxin production, with MystB being particularly important. Additionally, the catalytic domain and catalytic site of Myst are crucial for its acetyltransferase function, and mystB is involved in oxidative detoxification.
ENVIRONMENTAL MICROBIOLOGY
(2022)
