Article
Chemistry, Multidisciplinary
Juntao Zhao, Feng Li, Shutian Kong, Tao Chen, Hao Song, Zhiwen Wang
Summary: By synthesizing riboflavin and overexpressing the outer membrane cytochrome MtrC and the cell division inhibitor sulA, Shewanella oneidensis achieves enhanced extracellular electron transfer (EET). Moreover, a hybrid biofilm composed of riboflavin, multiwalled carbon nanotubes (MWCNTs), and graphene oxide (GO) significantly increases power generation and reduces charge-transfer resistance.
Article
Environmental Sciences
Fei Zhu, Yunhong Huang, Haiyan Ni, Jie Tang, Qi Zhu, Zhong-Er Long, Long Zou
Summary: Microbially driven iron and sulfur geochemical cycles are common in subsurface environments. Shewanella species have the ability to reduce Fe(III) minerals and sulfur sources, producing FeS secondary minerals. This study investigated the electron-mediating function of biogenic FeS in promoting the reduction of ferrihydrite by S. oneidensis MR-1 using thiosulfate as sulfur source, demonstrating that biogenic FeS can accelerate interfacial electron transfer between microbes and minerals.
Article
Biotechnology & Applied Microbiology
Yuan-Yuan Cheng, Wen-Jing Wang, Shi-Ting Ding, Ming-Xing Zhang, Ai-Guo Tang, Ling Zhang, Dao-Bo Li, Bing-Bing Li, Guo-Zhi Deng, Chao Wu
Summary: This study demonstrates that using pyruvate as a carbon source significantly enhances the reduction capability of Shewanella oneidensis towards palladium ions, by promoting electron generation and transfer. The mechanism of pyruvate regulation in this process includes modulation of gene expression related to pyruvate metabolism, electron transfer, and reduction reactions, requiring a 2-hour time window for gene regulation.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiaoxue Zhang, Mengmeng Zhong, Rui Zhou, Wenxiu Qin, Youbin Si
Summary: This study systematically investigated the reduction of Se(IV) by Shewanella oneidensis MR-1 and Shewanella putrefaciens. The results showed that excessive Se(IV) severely inhibited the growth of microorganisms and Se(IV) reduction. The synthesized Nano-Se(0) particles exhibited superior dispersibility and biocompatibility, making them suitable for various technological applications.
PROCESS BIOCHEMISTRY
(2023)
Article
Engineering, Environmental
Xiang Xiao, Chang-Xing Li, Jie-Ru Peng, Yang-Yang Fan, Wen-Wei Li
Summary: This study reveals the electron transfer mechanism of Shewanella oneidensis MR-1 exoelectrogens, and finds that the contribution of the CymA pathway is positively correlated with EET rate. These findings provide a new perspective for understanding bacteria-environment interactions and may inspire the discovery of new EET proteins and the development of more environmentally-robust biotechnologies.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Environmental Sciences
Zhen Yang, Tianran Sun, Andreas Kappler, Jie Jiang
Summary: This study reveals the role of biochar in mediating the extracellular electron transfer (EET) process by influencing the formation and transformation of cell EPS. The results show that biochar not only facilitates electron transfer but also increases the secretion of EPS, leading to faster reduction of iron minerals.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Electrochemistry
Yu-Ting Zhang, Yong Zhang, Luo Peng
Summary: In situ fluorescence microscopy and electrochemical measurements were used to study the electrode biofilm of Shewanella oneidensis MR-1, showing that current generation was positively correlated with electrode coverage by cells, and biofilm formation preceded the expression of redox proteins and current generation.
ELECTROCHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Yaru Chen, Xiaolong Niu, Meijie Cheng, Luxin Wang, Panxing Sun, Hao Song, Yingxiu Cao
Summary: In this study, a CRISPRa and CRISPRi system was developed for gene regulation in S.oneidensis, which significantly improved EET efficiency and accelerated dye degradation.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Environmental Sciences
Yi-Yan Yu, Yong Zhang, Luo Peng
Summary: Many exoelectrogens can use redox-active metabolites secreted by other organisms as redox mediators, facilitating extracellular electron transfer. The interaction between Shewanella oneidensis and phenazine 1-carboxylic acid (PCA) was studied, demonstrating that PCA can enhance oxidative current and inward electron transfer in Shewanella. This finding is important for electroactive bacteria living in open systems with low mediator concentrations.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Biochemistry & Molecular Biology
Weiwei Chang, Yangyang Li, Ziyu Li, Yuntian Lou, Tianyu Cui, Hongchang Qian, Arjan Mol, Dawei Zhang
Summary: The microbiologically influenced corrosion of pure iron in the presence of Shewanella oneidensis MR-1 was studied, and it was found that increasing levels of exogenous riboflavin (RF) resulted in the formation of a better protective layer on the surface of pure iron. Furthermore, the addition of RF accelerated the corrosion at the initial immersion stage, thereby accelerating the deposition of products to form a subsequent protective layer.
BIOELECTROCHEMISTRY
(2022)
Article
Microbiology
Lixia Fang, Yuanyuan Li, Yan Li, Yingxiu Cao, Hao Song
Summary: It has been found that the modulation of specific genes can improve the extracellular electron transfer rate and enhance electricity generation in Shewanella oneidensis.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Feng Li, Rui Tang, Baocai Zhang, Chunxiao Qiao, Huan Yu, Qijing Liu, Junqi Zhang, Liang Shi, Hao Song
Summary: Researchers systematically engineered Shewanella oneidensis, an exoelectrogenic model organism, to promote biofilm formation and enhance biofilm conductivity in microbial electrochemical systems. The engineered biofilm achieved a maximum output power density of 3.62 +/- 0.06 W m-2, 39.3 times higher than that of the wild-type strain, setting a new record for the output power density of genetically engineered Shewanella biofilms.
Article
Electrochemistry
Peng Zhang, Chenhui Yang, Zeng Li, Jia Liu, Xiang Xiao, Da Li, Chong Chen, Miao Yu, Yujie Feng
Summary: The addition of carbon dots (CDs) can increase the efficiency of microbial extracellular electron transfer (EET) by accelerating the electron transfer process. The concentration of CDs affects the EET efficiency, with an appropriate amount improving both direct and mediated EET processes, while an overload of CDs can restrict their promotion effect and be toxic to bacteria.
ELECTROCHIMICA ACTA
(2022)
Article
Environmental Sciences
Junqi Zhang, Deguang Wu, Yakun Zhao, Dingyuan Liu, Xuewu Guo, Cuiying Zhang, Xi Sun, Ju Guog, Dezhi Yuan, Dongguang Xiao, Feng Li, Hao Song
Summary: This study aimed to enhance power generation in microbial fuel cells by enabling S. oneidensis to co-utilize glucose and lactate from thin stillage. The researchers successfully constructed an engineered S. oneidensis strain and a 3D self-assembled biohybrid, resulting in the highest power density reported for genetically engineered S. oneidensis with thin stillage as a carbon source. This study provides a novel strategy for practical applications of microbial fuel cells in wastewater remediation and power recovery.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Geochemistry & Geophysics
Hang Cheng, Zehua Jing, Liu Yang, Anhuai Lu, Guiping Ren, Juan Liu
Summary: This study found that under dark conditions, hematite can promote Cr(VI) removal at low mineral-to-cell ratios, but can hinder the reduction of Cr(VI) with increased mineral ratios. Under simulated sunlight, S. oneidensis MR-1 generates electrons from lactate metabolism to reduce Cr(VI) with hematite.
GEOCHIMICA ET COSMOCHIMICA ACTA
(2021)
Article
Ecology
David J. F. Walker, Ramesh Y. Adhikari, Dawn E. Holmes, Joy E. Ward, Trevor L. Woodard, Kelly P. Nevin, Derek R. Lovley
Article
Biochemical Research Methods
Christopher M. Dundas, Austin J. Graham, Dwight K. Romanovicz, Benjamin K. Keitz
ACS SYNTHETIC BIOLOGY
(2018)
Article
Microbiology
David J. F. Walker, Eric Martz, Dawn E. Holmes, Zimu Zhou, Stephen S. Nonnenmann, Derek R. Lovley
Article
Biochemical Research Methods
Toshiyuki Ueki, David J. F. Walker, Pier-Luc Tremblay, Kelly P. Nevin, Joy E. Ward, Trevor L. Woodard, Stephen S. Nonnenmann, Derek R. Lovley
ACS SYNTHETIC BIOLOGY
(2019)
Review
Microbiology
Derek R. Lovley, David J. F. Walker
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Multidisciplinary Sciences
Sarah K. Springthorpe, Christopher M. Dundas, Benjamin K. Keitz
NATURE COMMUNICATIONS
(2019)
Article
Ecology
David J. F. Walker, Kelly P. Nevin, Dawn E. Holmes, Amelia-Elena Rotaru, Joy E. Ward, Trevor L. Woodard, Jiaxin Zhu, Toshiyuki Ueki, Stephen S. Nonnenmann, Michael J. McInerney, Derek R. Lovley
Article
Biochemical Research Methods
Toshiyuki Ueki, David J. F. Walker, Trevor L. Woodard, Kelly P. Nevin, Stephen S. Nonnenmann, Derek R. Lovley
ACS SYNTHETIC BIOLOGY
(2020)
Article
Materials Science, Biomaterials
Austin J. Graham, Christopher M. Dundas, Alexander Hillsley, Dain S. Kasprak, Adrianne M. Rosales, Benjamin K. Keitz
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2020)
Article
Multidisciplinary Sciences
Tianda Fu, Xiaomeng Liu, Hongyan Gao, Joy E. Ward, Xiaorong Liu, Bing Yin, Zhongrui Wang, Ye Zhuo, David J. F. Walker, J. Joshua Yang, Jianhan Chen, Derek R. Lovley, Jun Yao
NATURE COMMUNICATIONS
(2020)
Article
Ecology
Reinaldo E. Alcalde, Christopher M. Dundas, Yiran Dong, Robert A. Sanford, Benjamin Keith Keitz, Bruce W. Fouke, Charles J. Werth
Summary: Spatial concentration gradients of antibiotics can impact microbial survival and metabolic activity, with chemotaxis playing a crucial role in allowing bacteria to inhabit toxic regions. Efflux pumps, specifically RND pumps, were found to be important for microbial survival in batch conditions, but were not necessary for survival in antibiotic gradients in microfluidic studies. This research contributes to understanding how antibiotic spatial heterogeneity affects microbial response.
Article
Chemistry, Multidisciplinary
Youhong Guo, Christopher M. Dundas, Xingyi Zhou, Keith P. Johnston, Guihua Yu
Summary: This study introduces a novel antibacterial hydrogel that can efficiently disinfect water sources within a short period of time without the need for energy input, achieving over 99.999% disinfection efficiency. Additionally, the hydrogel exhibits excellent photothermal and biofouling-resistant properties, making it suitable for stable water purification as a solar evaporator under sunlight.
ADVANCED MATERIALS
(2021)
Editorial Material
Biotechnology & Applied Microbiology
Christopher M. Dundas, Benjamin K. Keitz
Summary: Light et al. discovered a previously unknown and widely conserved extracellular electron transfer pathway in the Gram-positive bacterium Listeria monocytogenes, which has significant implications for redox-driven biotechnologies.
TRENDS IN BIOTECHNOLOGY
(2023)