Review
Pharmacology & Pharmacy
Vesa M. Olkkonen
Summary: Oxysterol-binding protein-related proteins (ORPs) are a family of intracellular lipid binding/transfer proteins involved in lipid transport within cells. They have the ability to bind phosphatidylinositol polyphosphates (PIPs). Dysregulation of certain ORP family members, such as ORP3, -4, -5, and -8, is implicated in cancer growth through their effects on signaling events, protein interactions, and cellular lipid transport. This review discusses the functional evidence of ORPs in cancer growth and explores the potential of targeting ORPs for anti-cancer therapy.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Cell Biology
Yuichi Wakana, Kaito Hayashi, Takumi Nemoto, Chiaki Watanabe, Masato Taoka, Jessica Angulo-Capel, Maria F. Garcia-Parajo, Hidetoshi Kumata, Tomonari Umemura, Hiroki Inoue, Kohei Arasaki, Felix Campelo, Mitsuo Tagaya
Summary: The study reveals that in cholesterol-fed cells, SCAP interacts with the VAP-OSBP complex at ER-Golgi membrane contact sites to facilitate CARTS biogenesis. Knockdown of SCAP inhibits PI4P turnover and alters the subcellular distribution of the VAP-OSBP complex, ultimately leading to decreased CARTS formation.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Microbiology
Priyanka Bansal, Neelam Antil, Manish Kumar, Yoshiki Yamaryo-Botte, Rahul Singh Rawat, Sneha Pinto, Keshava K. Datta, Nicholas J. Katris, Cyrille Y. Botte, T. S. Keshava Prasad, Pushkar Sharma
Summary: In this study, we demonstrate that the protein kinase TgCDPK7 regulates cellular processes such as vesicular trafficking and phospholipid synthesis, critical for the development of the parasite Toxoplasma gondii. It phosphorylates the small GTPase TgRab11a to regulate the trafficking of important parasite proteins, crucial for parasite division. TgCDPK7 may also regulate key enzymes involved in the biogenesis of phosphatidylethanolamine, contributing to the synthesis of this important phospholipid. These findings shed light on a novel signaling pathway in apicomplexan parasite Toxoplasma gondii.
Review
Biochemistry & Molecular Biology
Melanie Galano, Sathvika Venugopal, Vassilios Papadopoulos
Summary: This review discusses the importance of cholesterol transport proteins and their roles in the trafficking and metabolism of cholesterol and other lipids in cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Jiang Min, Shenglan Yang, Yang Cai, David R. Vanderwall, Zhiping Wu, Shuping Li, Songlan Liu, Beibei Liu, Jie Wang, Yingjun Ding, Junxiong Chen, Chao Jiang, Jonathan D. Wren, Anna Csiszar, Zoltan Ungvari, Celine Greco, Tomoharu Kanie, Junmin Peng, Xin A. Zhang
Summary: In this study, it was found that Tspan8, a tetraspanin expressed specifically in epithelial cells, supports intestinal epithelial barrier and inhibits inflammation. Tspan8 determines the endocytosis route of IFN-gamma R1, leading to decreased Stat1 signaling. The findings suggest that Tspan8 plays a crucial role in stabilizing the intestine epithelium and preventing inflammation.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Kohta Takahashi, Kristiina Kanerva, Lauri Vanharanta, Leonardo Almeida-Souza, Daniel Lietha, Vesa M. Olkkonen, Elina Ikonen
Summary: The study reveals the mechanism by which ORP2 regulates lipid and protein trafficking in cells, controlling FAK activation and LDL-cholesterol plasma membrane delivery by facilitating cholesterol and PI(4,5)P-2 exchange between late and recycling endosomes.
Review
Biology
D. C. Ghislaine Mayer
Summary: This review discusses the latest findings on the molecular mechanisms of protein sorting and vesicular trafficking in P. falciparum, showing that the parasite has repurposed molecules of the endosomal system for its secretory pathway. The sorting and vesicular trafficking mechanism appears to be conserved among apicomplexans, indicating a clever modification of P. falciparum's secretory machinery for its intracellular lifestyle.
Article
Cell Biology
Melody Subra, Manuela Dezi, Joelle Bigay, Sandra Lacas-Gervais, Aurelie Di Cicco, Ana Rita Dias Araujo, Sophie Abelanet, Lucile Fleuriot, Delphine Debayle, Romain Gautier, Amanda Patel, Fanny Roussi, Bruno Antonny, Daniel Levy, Bruno Mesmin
Summary: Membrane contact sites (MCSs) are diverse in shape, composition, and dynamics. VAP proteins play a crucial role in the formation of MCSs involving the endoplasmic reticulum (ER). The localization and function of VAP-A in different MCSs depend on its intrinsically disordered regions (IDRs). IDRs do not affect VAP-A's preference for specific partners but adjust its geometry to MCS organization and lifetime constraints, ensuring membrane tethering plasticity and efficiency.
DEVELOPMENTAL CELL
(2023)
Article
Multidisciplinary Sciences
Jennifer L. Johnson, Elsa Meneses-Salas, Mahalakshmi Ramadass, Jlenia Monfregola, Farhana Rahman, Raquel Carvalho Gontijo, William B. Kiosses, Kersi Pestonjamasp, Dale Allen, Jinzhong Zhang, Douglas G. Osborne, Yanfang Peipei Zhu, Nathan Wineinger, Kasra Askari, Danni Chen, Juan Yu, Scott C. Henderson, Catherine C. Hedrick, Matilde Valeria Ursini, Sergio Grinstein, Daniel D. Billadeau, Sergio D. Catz
Summary: This study investigates the process of exocytosis in neutrophil leukocytes and shows that the actin nucleator protein WASH plays a critical role in this process. The authors find that WASH facilitates the release of gelatinase granules while inhibiting the release of pro-inflammatory granules. They also discover that a deficiency in WASH leads to dysregulated secretion and inflammation in neutrophil leukocytes.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Suzan Kors, Christian Hacker, Chloe Bolton, Renate Maier, Lena Reimann, Emily J. A. Kitchener, Bettina Warscheid, Joseph L. Costello, Michael Schrader
Summary: Studies have found that peroxisomes and the endoplasmic reticulum (ER) cooperate in cellular lipid metabolism through the interaction of ACBD5 and VAPB. This interaction is regulated by phosphorylation and GSK3 beta. This discovery provides new insights into the molecular mechanisms of peroxisome-ER contacts.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Genetics & Heredity
Lea Hentrich, Mered Parnes, Timothy Edward Lotze, Rohini Coorg, Tom J. de Koning, Kha M. Nguyen, Calvin K. Yip, Heinz Jungbluth, Anne Koy, Hormos Salimi Dafsari
Summary: Biallelic variants in the GOSR2 gene can lead to progressive myoclonus epilepsy with neurodegeneration. This study reports two new patients with GOSR2-related disorders and novel genetic and clinical findings.
Article
Biochemistry & Molecular Biology
Henrik Nurmi, Anders P. E. Backman, Josefin Halin, Max Lonnfors, Tomas Blom, Pia Roos-Mattjus, Peter Mattjus
Summary: The glycolipid transfer protein (GLTP) is involved in various cellular processes, including lipid transport, sensing and regulating glycosphingolipid homeostasis, and facilitating vesicular transport. Knocking out GLTP in HeLa cells affected motility, three-dimensional growth, and cellular metabolism. The interaction between GLTP and VAP-A is important for cell adhesion, cohesion, and three-dimensional growth, and GLTP expression directly impacts vesicular trafficking.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Plant Sciences
Tamara Pecenkova, Premysl Pejchar, Tomas Moravec, Matej Drs, Samuel Haluska, Jiri Santrucek, Andrea Potocka, Viktor Zarsky, Martin Potocky
Summary: PR1 protein, commonly used as a marker for biotic stress monitoring in plants, undergoes partial proteolytic processing en route from the endoplasmic reticulum, with only nonmutated variants or processing-mimicking ones being secreted to the apoplast. The released protein fragment acts as a modulator of plant defense responses, with tissue-localized and host plant ontogenesis-dependent immunity potential.
MOLECULAR PLANT PATHOLOGY
(2022)
Article
Virology
Jeffrey J. Hodgson, Nicolas Buchon, Gary W. Blissard
Summary: Little is known about the trafficking process of viral envelope proteins in insect cells. In this study, a stable Drosophila cell line was engineered to investigate the host proteins and cellular trafficking pathways involved in the delivery of the baculovirus envelope protein GP64 to the plasma membrane. Through a targeted RNA interference screen, several host genes were identified to be important for the trafficking of GP64 to the cell surface. The findings suggest that recycling plays a role in maintaining GP64 at the cell surface. This study provides insight into the understanding of how insect virus envelope proteins are transported and contributes to the knowledge of virus infection processes.
JOURNAL OF VIROLOGY
(2022)
Review
Multidisciplinary Sciences
Muneer Ahmed Khoso, Hailong Zhang, Mir Hassan Khoso, Tika Ram Poudel, Sindho Wagan, Tamar Papiashvili, Sudipta Saha, Abid Ali, Ghulam Murtaza, Hakim Manghwar, Fen Liu
Summary: The cytoskeleton is a crucial component in eukaryotic organisms, particularly in plant cells, playing a critical role in cellular mechanisms such as locomotion and morphological transformations. Comprised of microtubules, microfilaments, and intermediate filaments, the cytoskeleton regulates cell wall formation and remodeling. Recent studies highlight its importance in intracellular transport processes, and the flaws in its organization lead to abnormal organogenesis observed in plant mutants. Protein compartmentalization and abundance within cells are predominantly regulated by vesicle/membrane transport, involving various proteins. This review presents the latest research on the plant cytoskeleton and its role in vesicle transport, predicting future research directions and providing guidance for further investigation.
Article
Obstetrics & Gynecology
Paul A. Fowler, Panagiotis Filis, Siladitya Bhattacharya, Bruno le Bizec, Jean-Philippe Antignac, Marie-Line Morvan, Amanda J. Drake, Ugo Soffientini, Peter J. O'Shaughnessy
HUMAN REPRODUCTION
(2016)
Article
Endocrinology & Metabolism
Diane Rebourcet, Annalucia Darbey, Ana Monteiro, Ugo Soffientini, Yi Ting Tsai, Ian Handel, Jean-Luc Pitetti, Serge Nef, Lee B. Smith, Peter J. O'Shaughnessy
Article
Medicine, General & Internal
Zoe C. Johnston, Michelle Bellingham, Panagiotis Filis, Ugo Soffientini, Denise Hough, Siladitya Bhattacharya, Marc Simard, Geoffrey L. Hammond, Peter King, Peter J. O'Shaughnessy, Paul A. Fowler
Article
Biochemistry & Molecular Biology
Ugo Soffientini, Anna-Maria Caridis, Sharron Dolan, Annette Graham
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2014)
Article
Multidisciplinary Sciences
Cinzia Bernardi, Ugo Soffientini, Francesco Piacente, Michela G. Tonetti
Article
Medicine, General & Internal
Panagiotis Filis, Sabine Hombach-Klonisch, Pierre Ayotte, Nalin Nagrath, Ugo Soffientini, Thomas Klonisch, Peter O'Shaughnessy, Paul A. Fowler
Article
Biochemistry & Molecular Biology
Peter J. O'Shaughnessy, Jean Philippe Antignac, Bruno Le Bizec, Marie-Line Morvan, Konstantin Svechnikov, Olle Soder, Iuliia Savchuk, Ana Monteiro, Ugo Soffientini, Zoe C. Johnston, Michelle Bellingham, Denise Hough, Natasha Walker, Panagiotis Filis, Paul A. Fowler
Article
Gastroenterology & Hepatology
Anna Hadjihambi, Christos Konstantinou, Jan Klohs, Katia Monsorno, Adrien Le Guennec, Chris Donnelly, I. Jane Cox, Anjali Kusumbe, Patrick S. Hosford, Ugo Soffientini, Salvatore Lecca, Manuel Mameli, Rajiv Jalan, Rosa Chiara Paolicelli, Luc Pellerin
Summary: This study investigates the effects of NAFLD on cognition, behavior, and cerebral physiology using a diet-induced NAFLD model. The study suggests that NAFLD is associated with anxiety and depression-related behavior, brain tissue hypoxia, and metabolic alterations in microglial and astrocytic cells. The study also highlights the protective effect of Mct1 haploinsufficiency against NAFLD and associated brain dysfunction.
JOURNAL OF HEPATOLOGY
(2023)
Review
Reproductive Biology
Natasha Walker, Panagiotis Filis, Ugo Soffientini, Michelle Bellingham, Peter J. O'Shaughnessy, Paul A. Fowler
BIOLOGY OF REPRODUCTION
(2017)