Article
Biochemistry & Molecular Biology
Dongxue Su, Wenjuan Wang, Yongqiang Hou, Liyong Wang, Xianfu Yi, Cheng Cao, Yuejiao Wang, Huan Gao, Yue Wang, Chao Yang, Beibei Liu, Xing Chen, Xiaodi Wu, Jiajing Wu, Dong Yan, Shuqi Wei, Lulu Han, Shumeng Liu, Qian Wang, Lei Shi, Lin Shan
Summary: This study reveals the regulation of EZH2 abundance and function by USP7 in cancer cells, highlighting the potential of USP7 as a therapeutic target for cancer intervention. The physical association between USP7 and PRC2 complex plays a critical role in cell growth, proliferation, and tumorigenesis, with implications for histological grades and prognosis of tumor patients.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Medicine, Research & Experimental
Rabih Roufayel, Rabih Mezher, Kenneth B. Storey
Summary: Transcription factors like Rb-E2F and Cyclin-Cdk complexes play critical roles in regulating gene expression and cellular adaptations to stress conditions, particularly during cell cycle control in response to environmental stresses.
CURRENT MOLECULAR MEDICINE
(2021)
Article
Multidisciplinary Sciences
Ravish Rashpa, Natacha Klages, Domitille Schvartz, Carla Pasquarello, Mathieu Brochet
Summary: The study reveals the crucial role of the SKP1/Cullin1/FBXO1 complex in cell division and parasite-specific processes in Plasmodium.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Dana Saad, Cristina Paissoni, Antonio Chaves-Sanjuan, Marco Nardini, Roberto Mantovani, Nerina Gnesutta, Carlo Camilloni
Summary: E2F1 as a master regulator of cell-cycle progression binds DNA as a heterodimer with DP partners, with an asymmetric contribution of dynamics in the complex. Tumor mutations in the flexible regions of the complex could affect protein function context-dependently, suggesting fundamental contributions of DP proteins in distinct aspects of E2F biology.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Marios G. Koliopoulos, Claudio Alfieri
Summary: The cell cycle is a crucial biological process where CDKs act as an oscillator to regulate orderly progression through different phases. Cryo-electron microscopy has revolutionized our understanding of the molecular function and dynamics of key players in the cell cycle.
Article
Biology
Taylor P. Enrico, Wayne Stallaert, Elizaveta T. Wick, Peter Ngoi, Xianxi Wang, Seth M. Rubin, Nicholas G. Brown, Jeremy E. Purvis, Michael J. Emanuele
Summary: Cell cycle gene expression programs are dysregulated in cancer, and the RB-family of proteins play a key role in repressing cell cycle gene expression and inhibiting proliferation. Phosphorylation and ubiquitination are crucial in cell cycle control, and the SCFcyclin F pathway may regulate the RB-network by affecting p130 degradation, potentially leading to cell cycle dysregulation in human cancers.
Article
Behavioral Sciences
Huan Xie, Shigang Lv, Zhaozhen Wang, Xinzhang Yuan
Summary: This study revealed that E2F1 reduces miR-107 transcription to induce CCND1 upregulation, promoting the progression of glioma. Overexpression of miR-107 can inhibit cell proliferation, migration, invasion, promote apoptosis, reduce the activity of the Wnt/β-catenin pathway, thereby blocking tumor growth.
BRAIN AND BEHAVIOR
(2021)
Article
Multidisciplinary Sciences
Maria Paula Zappia, Yong-Jae Kwon, Anton Westacott, Isabel Liseth, Hyun Min Lee, Abul B. M. M. K. Islam, Jiyeon Kim, Maxim V. Frolov
Summary: The transcription factor E2F is responsible for controlling the expression of cell cycle genes by binding to their promoters. However, its significance in the regulation of metabolic genes is not well understood. In this study, the researchers introduced mutations in the E2F sites of five metabolic genes in fruit flies and found varying effects on E2F recruitment and gene expression, with the glycolytic gene Pgk being the most affected. Loss of E2F regulation on the Pgk gene led to decreased glycolytic flux, ATP content, and abnormal mitochondrial morphology. The study highlights the importance of E2F regulation on metabolic genes and its impact on overall development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Hongli Xu, Shengnan Liang, Junjie Hu, Wentong Liu, Zhiqiang Dong, Shaozhong Wei
Summary: DTX3 regulates colorectal cancer cell growth by regulating E2F1 and its downstream genes. These findings support further exploration of DTX3 as a potential therapeutic target for colorectal cancer.
MOLECULAR BIOLOGY REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Kevin G. Mark, Michael Rape
Summary: Research has shown that ubiquitylation plays a crucial role in transcription, ensuring the correct expression of genes during development. Disruption of these regulatory circuits can lead to a wide array of human diseases.
Article
Genetics & Heredity
Minhee Kim, Keemo Delos Santos, Nam-Sung Moon
Summary: Polyploidy is a crucial process in development, associated with cellular stress, aging, and pathological conditions. The endocycle, which involves repeated G and S phases without mitosis, is regulated by E2F and plays a key role in producing polyploid cells. In Drosophila, an alternative isoform of de2f1, dE2F1b, is shown to control the expression of the Cdk inhibitor Dap and is required for proper CycE-Cdk2 activity during the endocycle. Additionally, dE2F1b is involved in establishing a negative feedback loop in S phase through regulating PCNA expression.
Article
Biochemistry & Molecular Biology
Christine A. Mills, Xianxi Wang, Dhaval P. Bhatt, Paul A. Grimsrud, Jacob Peter Matson, Debojyoti Lahiri, Daniel J. Burke, Jeanette Gowen Cook, Matthew D. Hirschey, Michael J. Emanuele
Summary: This study identified SIRT5 as a novel substrate of Cyclin F, which regulates cell cycle and metabolism through interaction with Cyclin F. The knockout of SIRT5 results in changes in cell cycle signaling congruent with S and G2/M phase increase. These findings suggest a connection between SIRT5, cell cycle regulation, and metabolism.
MOLECULAR AND CELLULAR BIOLOGY
(2021)
Article
Genetics & Heredity
Hengwei Lu, Yi-Ching Tang, Assaf Gottlieb
Summary: Gene expression is crucial for understanding health and disease. Estimating the genetic components of gene expression can provide insights into disease etiology. In this study, a hypothesis-driven approach was used to identify variations in transcription factors (TFs) that can explain missing gene expression. The results showed that TF variability can partially explain missing gene expression in tissue-specific patterns, and these patterns were enriched for immune system functionality.
Article
Cell Biology
Wenjing Xiao, Jianqun Wang, Xiaojing Wang, Shuang Cai, Yanhua Guo, Lin Ye, Dan Li, Anpei Hu, Shikai Jin, Boling Yuan, Yi Zhou, Qilan Li, Qiangsong Tong, Liduan Zheng
Summary: The study identified E2F4 as a novel transcriptional activator of cytoprotective autophagy crucial for zinc homeostasis in cancer cells. The interaction between USP2 and E2F4 plays a significant role in regulating autophagy and zinc balance. Targeting the USP2-E2F4 axis could inhibit autophagic machinery essential for cancer progression.
Article
Biochemistry & Molecular Biology
Robert Duester, Yanlong Ji, Kuan-Ting Pan, Henning Urlaub, Matthias Geyer
Summary: In this study, the substrate specificity and function of Cdk10 were thoroughly analyzed, revealing its important role in cell cycle regulation and transcriptional regulation. Cdk10 can act as a tumor suppressor and potentially interact with other CDKs.
Article
Cell Biology
Danit Wasserman, Sapir Nachum, Meital Cohen, Taylor P. Enrico, Meirav Noach-Hirsh, Jasmin Parasol, Sarit Zomer-Polak, Naomi Auerbach, Evelin Sheinberger-Chorni, Hadas Nevenzal, Nofar Levi-Dadon, Xianxi Wang, Roxane Lahmi, Efrat Michaely, Doron Gerber, Michael J. Emanuele, Amit Tzur
MOLECULAR BIOLOGY OF THE CELL
(2020)
Article
Materials Science, Biomaterials
Inna Desyatnik, Matan Krasner, Ludmila Frolov, Maria Ronen, Ortal Guy, Danit Wasserman, Amit Tzur, Dorit Avrahami, Efrat Barbiro-Michaely, Doron Gerber
ADVANCED BIOSYSTEMS
(2019)