Article
Cell Biology
Lei Luo, Yanna Dang, Yan Shi, Panpan Zhao, Yunhai Zhang, Kun Zhang
Summary: SIN3A is a central scaffold protein involved in the regulation of HDAC1 expression during early embryonic development in mammals. The maternal origin of SIN3A was confirmed in pigs, cattle, mice, and humans, with its depletion causing developmental arrest in pigs but not in bovines. Furthermore, SIN3A affects CCNB1 expression, which is crucial for embryonic development, and can be partially rescued by exogenous CCNB1 mRNA injection.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Baohua Tan, Sheng Wang, Shanshan Wang, Jiekang Zeng, Linjun Hong, Zicong Li, Jie Yang, Gengyuan Cai, Enqin Zheng, Zhenfang Wu, Ting Gu
Summary: This study provided a comprehensive genome-wide view of H3K27me3 during porcine embryonic muscle development, revealing its continuous increase and negative correlation with gene expression. It also showed that H3K27me3 could enrich in the promoter to negatively regulate gene expression and impact muscle cell differentiation in pigs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Amanda Soares Jorge, Kaiana Recchia, Mayra Hirakawa Gloria, Aline Fernanda de Souza, Lais Vicari de Figueiredo Pessoa, Paulo Fantinato Neto, Daniele dos Santos Martins, Andre Furugen Cesar de Andrade, Simone Maria Massami Kitamura Martins, Fabiana Fernandes Bressan, Naira Caroline Godoy Pieri
Summary: This study examines the dynamics of pluripotent, germline, and epigenetic markers in porcine germ cells during development and provides insights into the potential applications in reproductive and regenerative medicine.
Article
Agriculture, Dairy & Animal Science
Weini Shao, Wei Ning, Chang Liu, Yuanyuan Zou, Yurui Yao, Jiaxin Kang, Zubing Cao
Summary: SETD2 plays a critical role in porcine early embryonic development by regulating H3K36me3 modification. Knockdown of SETD2 severely inhibits blastocyst cavitation and disrupts the allocation between inner cell mass and trophectoderm, leading to increased apoptosis of cells within the embryos.
Article
Multidisciplinary Sciences
Alexander Vietheer, Torvid Kiserud, Oystein Ariansen Haaland, Rolv Terje Lie, Joerg Kessler
Summary: Sleep duration before pregnancy has an impact on fetal development, with differences seen in yolk sac and crown-rump-length measurements in male fetuses but not female fetuses at 7 weeks. These findings suggest a sex- and time-dependent embryonic adaptation to sleep affecting the intrauterine environment in normal pregnancies.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Dongjie Zhou, Ming-Hong Sun, Wen-Jie Jiang, Xiao-Han Li, Song-Hee Lee, Geun Heo, Jungseok Choi, Kwan-Suk Kim, Xiang-Shun Cui
Summary: In this study, the mRNA level of YME1L1 was knocked down in pig embryos and the expression patterns of YME1L1 and related proteins were examined. It was found that YME1L1 is localized in the punctate structures of mitochondria and highly expressed from the 4-cell stage. Knocking down YME1L1 led to decreased blastocyst rate and quality, mitochondrial fragmentation, excessive ROS production, lower mitochondrial membrane potential and ATP levels. Moreover, YME1L1 knockdown induced OPA1 cleavage and cytochrome c release. These findings demonstrate that YME1L1 is crucial for regulating mitochondrial fission, function, and apoptosis during porcine embryo preimplantation development.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Reproductive Biology
Yue Wang, Chun-Hua Xing, Shun Chen, Shao-Chen Sun
Summary: The study showed that ZEN exposure disrupted organelle function in porcine oocyte meiotic maturation, affecting mitochondria, endoplasmic reticulum, Golgi apparatus, and lysosome.
Article
Cell Biology
Xiangyu Wang, Mingju Sun, Jingyu Li, Xuexiong Song, Hongbin He, Yanjun Huan
Summary: Exogenous factor EB1 was found to have detrimental effects on porcine early embryos, while melatonin showed effectiveness in rescuing the defects caused by EB1. This study provides new insights into enhancing embryo quality and promoting human and animal reproduction.
Article
Oncology
Ang Li, Fei Li, Wei Song, Zi-Li Lei, Qian-Qian Sha, Shao-Yuan Liu, Chang-Yin Zhou, Xue Zhang, Xiao-Zhen Li, Heide Schatten, Teng Zhang, Qing-Yuan Sun, Xiang-Hong Ou
Summary: This study investigated the relationship between gut microbiota and oocyte quality decline caused by circadian rhythm disruption. The results showed that the decreased absorption capacity of vitamin D in the intestine, as a result of reduced levels of bile acids such as lithocholic acid (LCA) and increased abundance of Turicibacter, led to decreased oocyte quality and embryonic development. The concentrations of anti-Mullerian hormone (AMH) hormone in blood and melatonin in follicular fluid were also reduced, contributing to the decline in oocyte quality and early embryonic development. Injection of vitamin D3 (VD3) or specific metabolites was able to rescue the oocyte quality and embryonic development. Overall, this study highlights the importance of gut microbiota regulation by microbial metabolites in improving ovary function.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Agriculture, Dairy & Animal Science
Lepeng Gao, Chang Zhang, Yingying Zheng, Deyi Wu, Xinyuan Chen, Hainan Lan, Xin Zheng, Hao Wu, Suo Li
Summary: The addition of glycine in in vitro culture can improve the maturation efficiency of oocytes by maintaining redox balance and blocking reactive oxygen species-induced lipid peroxidation. This study provides a theoretical basis for preventing and improving oxidative damage during oocyte culture in vitro.
JOURNAL OF ANIMAL SCIENCE
(2023)
Article
Cell Biology
Tianqi Zhu, Shengyu Guan, Dongying Lv, Mengmeng Zhao, Laiqing Yan, Li Shi, Pengyun Ji, Lu Zhang, Guoshi Liu
Summary: The study established methods to monitor lipid metabolism in single live oocytes using probes. It was found that both lipid biogenesis and lipolysis occurred during oocyte maturation, but the total lipid level decreased. Melatonin rescued disrupted lipid metabolism and maturation in porcine oocytes treated with certain inhibitors, highlighting its potential in improving oocyte developmental competence. Additionally, the presence of melatonin receptors in cumulus cells and their impact on gene expression related to antioxidants, lipid metabolism, and mitochondrial respiration were observed, providing further insights into the mechanism of melatonin's effect on oocyte maturation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Ming-Hong Sun, Wen-Jie Jiang, Xiao-Han Li, Song-Hee Lee, Geun Heo, Dongjie Zhou, Jung-Seok Choi, Kwan-Suk Kim, Wenfa Lv, Xiang-Shun Cui
Summary: The study discovered the important role of ATF7 in porcine embryonic development, where it affects embryonic development by regulating heterochromatin formation. Inhibiting ATF7 leads to decreased embryo quality and cell number, and is associated with P38 activity. High temperatures induce ATF7 phosphorylation and reduce the expression level of heterochromatin-associated proteins.
CELL PROLIFERATION
(2023)
Article
Biochemistry & Molecular Biology
Agostinho Soares de Alcantara-Neto, Cristina Cuello, Rustem Uzbekov, Stefan Bauersachs, Pascal Mermillod, Carmen Alminana
Summary: This study determined the functional impact of oviductal extracellular vesicles (oEVs) on porcine embryo development and transcriptome. The results showed that treatment with oEVs enhanced embryo development and led to differential gene and miRNA expression profiles in the embryos. Specific oEV components, such as S100A11, ANXA2, and miR-21-5p, were identified as potential modulators of the embryonic transcriptome.
Article
Reproductive Biology
Jiannan Li, Ruibin Wang, Qiang Chen, Yiqi Tian, Leilei Gao, Anmin Lei
Summary: Salidroside (SAL) improves the maturation and subsequent development of porcine oocytes by promoting lipid metabolism and providing energy, resulting in increased blastocyst formation rate and embryo quality.
Article
Biochemistry & Molecular Biology
Kamila Martyniuk, Maria Hanuszewska-Dominiak, Bogdan Lewczuk
Summary: Research on age-dependent changes in pineal activity has mainly focused on melatonin. This study found significant alterations in the metabolic profile of indoles related to melatonin synthesis during post-embryonic development in birds, which could be categorized into four stages based on age.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.