Article
Food Science & Technology
Xueran Geng, Jiayu Lei, Tergun Bau, Dongdong Guo, Mingchang Chang, Cuiping Feng, Lijing Xu, Yanfen Cheng, Ningke Zuo, Junlong Meng
Summary: In this study, a novel acidic alpha-galactosidase was purified from Oudemansiella radicata and further immobilized to obtain iORG. The immobilized enzyme showed higher stability and better degradation effect on raffinose family oligosaccharides (RFOs) in soymilk, indicating its potential application in the food industry.
Article
Biochemistry & Molecular Biology
Bing Hua, Mengying Zhang, Jinji Zhang, Haibo Dai, Zhiping Zhang, Minmin Miao
Summary: In cucumber fruits, RFOs hydrolysis mainly occurs in the vascular tissues. CsAGA1 and CsAGA2 play key but partly distinct roles in the hydrolysis of RFOs, with three alkaline forms of alpha-Gals being preferentially expressed in fruit vascular tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Evran Bicak Celem, Secil Onal
Summary: The immobilized alpha-galactosidase can effectively hydrolyze RFOs in soymilk, thus improving its nutritional value.
PROCESS BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Xixi Bai, Xiaoyang Sun, Yue Yu, Yuheng Guo, Linyu Nian, Chongjiang Cao, Shujie Cheng
Summary: In this study, a PVA-CS-GMA material was successfully synthesized and used to immobilize α-galactosidase. The resulting material had a stable porous network and showed suitable mechanical properties and high water content for retaining catalytic activity. The immobilized α-galactosidase exhibited improved enzymatic properties and reusability compared to the free enzyme. It was successfully applied in the hydrolysis of RFOs in soybeans, providing a new strategy for diet intervention in IBS.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Agriculture, Multidisciplinary
Yumin Zhang, Dan Li, Lynnette M. A. Dirk, A. Bruce Downie, Tianyong Zhao
Summary: ZmAGA1 overexpression in Arabidopsis reduces RFOs and galactinol content in seeds, leading to increased seed germination percentage under stress conditions, but decreased seed aging tolerance. Manipulation of ZmAGA1 expression can potentially regulate maize seed germination and aging tolerance simultaneously.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Martina Aulitto, Andrea Strazzulli, Ferdinando Sansone, Flora Cozzolino, Maria Monti, Marco Moracci, Gabriella Fiorentino, Danila Limauro, Simonetta Bartolucci, Patrizia Contursi
Summary: This study demonstrates the ability of B. coagulans MA-13 to over-produce α-galactosidase and β-galactosidase, which are of great importance to the food industry. This paves the way for potential use in treating gastrointestinal diseases.
MICROBIAL CELL FACTORIES
(2021)
Article
Biochemistry & Molecular Biology
Purvi Bangoria, Sunil Chaki, Amita R. Shah
Summary: In this study, partially purified alpha-galactosidase from Penicillium aculeatum APS1 was successfully immobilized on chitosan coated magnetic nanoparticles. The immobilization conditions were optimized and the immobilized enzyme showed improved thermostability and storage stability. The immobilized enzyme was also effective in removing raffinose family oligosaccharides (RFO) in soymilk.
BIOCATALYSIS AND BIOTRANSFORMATION
(2023)
Article
Biochemistry & Molecular Biology
Yu Fang, Meihong Dong, Sander S. van Leeuwen, Lubbert Dijkhuizen, Xiangfeng Meng, Weifeng Liu
Summary: This study explored two α-glucosidases (MT31a1 and MT31a2) from Myceliophthora thermophila and characterized their biochemical properties. MT31a1 displayed high thermostability and was able to synthesize main functional IMOs components from maltose. MT31a2, on the other hand, preferred substrates with α-(1?3) and α-(1?4) linkages. Both enzymes show potential for the synthesis of prebiotic glucooligosaccharides.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Applied
Esin Calci, Secil Onal
Summary: This study presents a new strategy for enzyme immobilization, called bioaffinity immobilization, which utilizes biospecific affinity interactions between enzymes and matrices. The authors immobilized alpha-galactosidase on chitosan functionalized with Concanavalin A using three different methods. The results showed that each method had different immobilization yields and activity levels, with method II achieving the highest immobilization yield and method III exhibiting the greatest hydrolysis efficiency for raffinose. The immobilized enzymes also demonstrated promising biochemical characterization and stability, indicating potential applications in the food and feed industries.
REACTIVE & FUNCTIONAL POLYMERS
(2022)
Article
Biotechnology & Applied Microbiology
Weizhe Wang, Sudun, Huizhong Hu, Jieran An, Hongxing Zhang, Zigang Zhao, Yanling Hao, Zhengyuan Zhai
Summary: This study investigates the impact of raffinose on the growth of L. salivarius and identifies the genes responsible for raffinose transport and hydrolysis. Results show that the RafP gene plays an important role in raffinose transport, while GalA1 is the primary enzyme involved in raffinose hydrolysis.
Review
Plant Sciences
Shijuan Yan, Qing Liu, Wenyan Li, Jianbing Yan, Alisdair R. Fernie
Summary: Raffinose family oligosaccharides (RFOs) play pivotal roles in regulating plant responses to various stresses, seed germination, plant development, and biotic stress resistance.
CRITICAL REVIEWS IN PLANT SCIENCES
(2022)
Article
Plant Sciences
Huan Liu, Xin Liu, Yalong Zhao, Jing Nie, Xuehui Yao, Lijun Lv, Junwei Yang, Ning Ma, Yicong Guo, Yaxin Li, Xueyong Yang, Tao Lin, Xiaolei Sui
Summary: Sugars are necessary for the growth and development of cucumber plants. CsAGA2, an alkaline alpha-galactosidase 2 gene, plays a key role in cucumber fruit development through the transportation of sugars in the vascular bundle. CsAGA2 expression increases gradually during fruit development, especially in the fruit vasculature. Downregulation of CsAGA2 leads to delayed fruit growth, while overexpression results in bigger fruits. Genes related to sugar metabolism, cell wall metabolism, and hormone signaling are downregulated in CsAGA2-RNAi plants. CsAGA2 also has a negative feedback regulation on source leaves. These findings suggest that CsAGA2 is essential for cucumber fruit set and development through mediation of sugar communication.
Article
Agricultural Engineering
Ling Liu, Xiaolong Wu, Weibo Sun, Xiang Yu, Taku Demura, Dawei Li, Qiang Zhuge
Summary: Plants respond to abiotic stress through transcriptional and metabolic adjustments, with Raffinose family oligosaccharides (RFOs) and the key enzyme galactinol synthase (GolS) playing crucial roles. The GolS of Populus trichocarpa shares common features with GolS proteins in other plants and contains stress-responsive elements in its promoter region. Overexpressing AtGolS2 and PtrGolS3 in poplar enhances stress tolerance and increases the accumulation of stress-related metabolites, suggesting these genes as potential candidates for improving abiotic stress tolerance in woody plants.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Agriculture, Multidisciplinary
Judith Keller, Sven Peko Marmit, Mirko Bunzel
Summary: The study characterized the dietary fiber fractions of whole seeds from different lupin species, finding that the soluble dietary fiber fraction consists mainly of stachyose and verbascose, while the insoluble dietary fiber is mainly composed of cellulose and pectins. The presence of xylans, xyloglucans, and fucosylated xyloglucans was also demonstrated, along with tissue-related differences in cellulose and RGI portions.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Freeha Kanwal, Dingxin Ren, Wajiha Kanwal, Mengying Ding, Junqing Su, Xiaoya Shang
Summary: Based on scientific health claims, prebiotics are important in the food and pharmaceutical industries. Different types of prebiotics have diverse effects on the host. Raffinose family oligosaccharides (RFOs) like raffinose, stachyose, and verbascose have been widely used in medicine, cosmetics, and food additives. They prevent the colonization of pathogens and provide nutrition for a healthy immune system. Enrichment of RFOs in healthy foods can enhance the gut microecology and have beneficial effects on the host's multiorgan systems. This review paper summarizes the sources of RFOs, their metabolism, and the health benefits of bifidobacterial carbohydrate utilization.
