☆ 4.3 Article

Synthesis of bio-mediated silver nanoparticles from Silybum marianum and their biological and clinical activities

MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS (2020)

Journal

MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

Volume 112, Issue -, Pages -

Publisher

ELSEVIER

DOI: 10.1016/j.msec.2020.110889

Keywords

Silver nanoparticles; Green synthesis; Silybum; Antimicrobial; Anti-diabetic; Anti-inflammatory

Ask authors/readers for more resources

Protocol

Community support

Reagent

Community support

Abstract

The purpose of current study was green synthesis of silver nanoparticles (AgNPs) from seeds and wild Silybum plants in comparison with their respective extracts followed by characterization and biological potency. The biologically synthesized AgNPs were subjected to characterization using techniques like XRD, FTIR, TEM, HPLC and SPE. Highly crystalline and stable NPs were obtained using Silybum wild plant (NP1) and seeds (NP3) with size range between 18.12 and 13.20 nm respectively. The synthesized NPs and their respective extracts revealed a vast range of biological applications showing antibacterial, antioxidant, anti-inflammatory, cytotoxic and antiaging potencies. The highest antioxidant activity (478.23 +/- 1.9 mu M, 176.91 +/- 1.3 mu M, 83.5 +/- 1.6% mu gAAE/mg, 156.32 +/- 0.6 mu gAAE/mg) for ABTS, FRAP, FRSA, TRP respectively was shown by seed extract (NP4) followed by highest value of (117.35 +/- 0.9 mu gAAE/mg) for TAC by wild extract (NP2). The highest antifungal activity (13 mm +/- 0.76) against Candida albicans was shown by NP3 while antibacterial activity of (6 mm against Klebsiella pneumonia) was shown by NP3 and NP4. The highest anti-inflammatory activity (38.56 +/- 1.29 against COX1) was shown by NP2. Similarly, the high value of (48.89 +/- 1.34 against Pentosidine-Like AGEs) was shown by NP4. Also, the high anti-diabetic activity (38.74 +/- 1.09 against a-amylase) was shown by NP4. The extracts and the synthesized NPs have shown activity against hepato-cellular carcinoma (HepG2) human cells. The HPLC analysis revealed that the highest value of silymarin component (silybin B 2289 mg/g DW) was found for NP4. Silydianin is responsible for capping. Among the green synthesized AgNPs and the extracts used, the effect of NP4 was most promising for further use.

Authors

I am an author on this paper

Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.3

Not enough ratings

Secondary Ratings

Novelty

Significance

Scientific rigor

Rate this paper

Green inspired synthesis of ZnO nanoparticles and its characterizations with biofilm, antioxidant, anti-inflammatory, and anti-diabetic activities

K. Velsankar, A. Venkatesan, P. Muthumari, S. Suganya, S. Mohandoss, S. Sudhahar

Summary: In recent times, there has been growing interest in the simple, eco-friendly, and cost-effective synthesis of nanoparticles through green technology. This report describes the synthesis of ZnO nanoparticles through both green and chemical routes. The green synthesis utilized Erythrina variegata leaf extract as a capping and stabilizing agent, while the chemical synthesis employed sodium hydroxide as a reducing agent. Various analytical techniques were used to characterize the green synthesized ZnO nanoparticles, including UV-visible, XRD, FTIR, DLS, HR-TEM, EDX, and mapping analyses. The results demonstrated the high crystalline nature of the nanoparticles, their surface plasmon resonance band, and the presence of Zn and O elements. The nanoparticles also exhibited strong antibacterial, antioxidant, anti-inflammatory, and anti-diabetic activities. These findings highlight the potential applications of green synthesized ZnO nanoparticles as antibacterial and antioxidant agents.

JOURNAL OF MOLECULAR STRUCTURE (2022)