Journal
JOURNAL OF CELL SCIENCE
Volume 133, Issue 15, Pages -Publisher
COMPANY BIOLOGISTS LTD
DOI: 10.1242/jcs.243303
Keywords
Centromere; CENP-B; ASH1L; HP1; Alternative epigenetic states; Acidic-rich domain
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) KAKENHI [16H04747, 16H01414, 18H04721]
- Kazusa DNA Research Institute Foundation
- Wellcome Trust [073915]
- Japan Society for the Promotion of Science (JSPS)
- Ministry of Education, Culture, Sports, Science and Technology
- Intramural Research Program of the National Institutes of Health, National Cancer Institute, Center for Cancer Research
- Grants-in-Aid for Scientific Research [16H01414, 18H04721, 16H04747] Funding Source: KAKEN
Ask authors/readers for more resources
CENP-B binds to CENP-B boxes on centromeric satellite DNAs (known as alphoid DNA in humans). CENP-B maintains kinetochore function through interactions with CENP-A nucleosomes and CENP-C. CENP-B binding to transfected alphoid DNA can induce de novo CENP-A assembly, functional centromere and kinetochore formation, and subsequent human artificial chromosome (HAG) formation. Furthermore, CENP-B also facilitates H3K9 (histone H3 lysine 9) trimethylation on alphoid DNA, mediated by Suv39h1, at ectopic alphoid DNA integration sites. Excessive heterochromatin invasion into centromere chromatin suppresses CENP-A assembly. It is unclear how CENP-B controls such different chromatin states. Here, we show that the CENP-B acidic domain recruits histone chaperones and many chromatin modifiers, including the H3K36 methylase ASH1L, as well as the heterochromatin components Suv39h1 and HP1 (HP1 alpha, beta and gamma, also known as CBX5, CBX1 and CBX3, respectively). ASH1L facilitates the formation of open chromatin competent for CENP-A assembly on alphoid DNA. These results indicate that CENP-B is a nexus for histone modifiers that alternatively promote or suppress CENP-A assembly by mutually exclusive mechanisms. Besides the DNA-binding domain, the CENP-B acidic domain also facilitates CENP-A assembly de novo on transfected alphoid DNA. CENP-B therefore balances CENP-A assembly and heterochromatin formation on satellite DNA.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available