Article
Biochemistry & Molecular Biology
Shiwei Wang, Yuqi Feng, Xiaofeng Han, Xinyu Cai, Liu Yang, Chaolan Liu, Lixin Shen
Summary: The active ingredient wogonin from Herb Paris was found to inhibit the quorum sensing system of P. aeruginosa PAO1, reducing virulence factor production and biofilm formation, and attenuating pathogenicity. This suggests wogonin as a potential candidate drug for combating P. aeruginosa infections by interfering with quorum sensing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Jieying Pu, Shebin Zhang, Xi He, Jianming Zeng, Cong Shen, Yanfen Luo, Honglin Li, Yifei Long, Jianping Liu, Qian Xiao, Yang Lu, Bin Huang, Cha Chen
Summary: A novel QS regulatory sRNA AmiL has been identified in P. aeruginosa, which influences diverse QS-mediated virulence factors and regulates QS gene expression and C4-HSL production.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Guanhua Xuan, Hong Lin, Lin Tan, Gang Zhao, Jingxue Wang
Summary: Quorum sensing (QS) plays a crucial role in regulating interactions between bacteria, including phage-bacterium interactions. This study showed that Pseudomonas aeruginosa PAO1 uses las QS to increase phage infection rates by upregulating the expression of galU, which is necessary for synthesizing phage receptor lipopolysaccharides. Inhibition of QS may reduce therapeutic efficacy of phages and affect the balance between antibiotic treatment and phage therapy.
Article
Microbiology
Wen-Ru Li, Tao-Hua Zeng, Zhi-Qing Zhang, Qing-Shan Shi, Xiao-Bao Xie
Summary: In this study, it was found that geraniol can inhibit the growth of Pseudomonas aeruginosa, prolong the lag phase and growth period. Geraniol also suppresses the expression of the three quorum sensing systems of P. aeruginosa, thereby attenuating the virulence factors regulated by these systems. This research is significant for improving the treatment of bacterial infections caused by P. aeruginosa.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Guanhua Xuan, Chuanjuan Lv, Huangwei Xu, Kai Li, Huaiwei Liu, Yongzhen Xia, Luying Xun
Summary: Sulfane sulfur is a common cellular component that positively correlates with LasR activity in P. aeruginosa PAO1, affecting gene transcription activation. While modifications by sulfane sulfur enhance LasR activity, H2O2 inhibits LasR via disulfide bond formation.
Article
Immunology
Sanket Kumar, Balwant S. Paliya, Brahma N. Singh
Summary: The phyto-synthesized silver nanoparticles (Ka-AgNPs) effectively inhibit QS-regulated virulence factors and biofilm formation in drug-resistant P. aeruginosa (PAO1) without affecting cell viability.
MICROBIAL PATHOGENESIS
(2022)
Review
Chemistry, Medicinal
Qiaoqiang Li, Shen Mao, Hong Wang, Xinyi Ye
Summary: The survival pressure caused by antibiotics prompts bacteria to develop drug resistance. Quorum sensing is an intercellular communication system that regulates bacterial virulence and biofilm formation. In Pseudomonas aeruginosa, the formation and diffusion of biofilm is closely related to the quorum sensing system. Quorum-sensing inhibitors can reduce bacterial toxicity and enhance sensitivity to antibiotics, making them a popular topic for research and development in anti-infection. This review provides an overview of the Pseudomonas aeruginosa quorum-sensing system and quorum-sensing inhibitors research, which will aid in the future development of novel inhibitors.
Article
Biotechnology & Applied Microbiology
Aishwarya Vetrivel, Preethi Vetrivel, Kavitha Dhandapani, Santhi Natchimuthu, Monica Ramasamy, Soundariya Madheswaran, Rajeswari Murugesan
Summary: The LasR inhibitors identified in this study were evaluated for their antibiofilm and antiquorum sensing activities against Pseudomonas aeruginosa PAO1. The selected compounds showed potent antibiofilm activity at sub-MICs without affecting the growth of P. aeruginosa PAO1. They also inhibited the production of quorum sensing-mediated virulence factors and exhibited lower cytotoxicity against peripheral blood lymphocytes. This study could pave a way to explore these compounds for the development of therapeutic agents against Pseudomonas aeruginosa biofilm-related infections.
INTERNATIONAL MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Xiaoyan Cheng, Mingqi Lu, Huifang Qiu, Yuanhao Li, Linfeng Huang, Weijun Dai
Summary: In this study, the QS phenotypes and genetic basis of laboratory PAO1 strains were systematically investigated using QS reporter and whole-genome sequencing (WGS). It was found that the PAO1-z strain carries an inactive LasR protein but an active Rhl QS system, and an 18-bp insertion in the mexT gene gives rise to the active QS system in the PAO1-z strain.
Article
Immunology
Himani Meena, Rashmi Mishra, Sampathkumar Ranganathan, V. Venkateswara Sarma, Dinakara Rao Ampasala, Busi Siddhardha
Summary: The crude extract of endophytic fungus Colletotrichum gloeosporioides HM3 isolated from Carica papaya leaves showed potential for inhibiting the virulence factors and biofilm production of Pseudomonas aeruginosa PAO1. The study revealed a significant decrease in the production of pathogenic determinants and a reduction in the ability of bacteria to form dense biofilm structures after treatment with the fungal extract. In silico analysis demonstrated the binding efficiency of bioactive compounds with QS transcriptional regulators, suggesting the metabolites derived from C. gloeosporioides HM3 could be considered as potential inhibitors of QS regulated virulence factors.
MICROBIAL PATHOGENESIS
(2021)
Article
Plant Sciences
Muzamil Ahmad Rather, Kuldeep Gupta, Manabendra Mandal
Summary: The study evaluated the inhibitory effect of methanol extract of Cuphea carthagenensis on biofilm and quorum sensing (QS) activity in Pseudomonas aeruginosa, demonstrating a significant suppression of biofilm formation and virulence factors.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Microbiology
Zhidong Zhang, Yang Sun, Yuanyang Yi, Xiaoyu Bai, Liying Zhu, Jing Zhu, Meiying Gu, Yanlei Zhu, Ling Jiang
Summary: A strain of Streptomyces with quorum-sensing inhibitory activity was isolated from the Kumutag Desert in Xinjiang, and it effectively inhibited biofilm formation and virulence factor production in bacteria such as Pseudomonas aeruginosa.
Article
Microbiology
Mengmeng Cheng, Ruiyi Chen, Lisheng Liao
Summary: This study investigates the impact of a gene variation, LasR(Q45stop), in Pseudomonas aeruginosa. The introduction of LasR(Q45stop) in the bacteria led to the development of elastase-phenotypic-variability variants during co-evolution with the wild-type. The variation in T2SS-peptidase xcpA and mexT genes played a crucial role in the divergence of various phenotypes. These findings highlight the ongoing microevolution of individual mutational trajectory diversity.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Huaqiao Tang, Suqi Hao, Muhammad Faraz Khan, Ling Zhao, Fei Shi, Yinglun Li, Hongrui Guo, Yuanfeng Zou, Cheng Lv, Jie Luo, Ze Zeng, Qiang Wu, Gang Ye
Summary: The study demonstrated that EGCG effectively alleviated lung injury and increased survival rates in mice infected with P. aeruginosa. EGCG improved lung damage by inhibiting the production of QS-related virulence factors.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zsofia Berkl, Ildiko Fekete-Kertesz, Kata Buda, Emese Vaszita, Eva Fenyvesi, Lajos Szente, Monika Molnar
Summary: Quorum sensing (QS) is a communication process in microorganisms that coordinates their activities through producing and detecting low-molecular-weight signal molecules. Cyclodextrins (CDs) can reduce signal communication and inhibit biofilm formation in pathogenic bacteria. The size, structure, and concentration of CDs are identified as key factors influencing biofilm formation. Alpha-CD and its random methylated and polymer derivatives demonstrate outstanding inhibitory efficiency.
Article
Genetics & Heredity
Gang Zhou, Ying-si Wang, Hong Peng, Su-juan Li, Ting-li Sun, Cai-ling Li, Qing-shan Shi, Xiao-bao Xie
Summary: This study reveals the importance of the ompX gene in Citrobacter werkmanii, showing that its deletion affects biofilm formation, swimming ability, susceptibility to bactericides, and response to calcium and magnesium stress. The ompX gene also interacts with other genes and is involved in various biological functions. This research provides valuable insights into controlling biofilm formation and developing new bactericides.
Article
Biotechnology & Applied Microbiology
Wen-Ru Li, Zhi-Qing Zhang, Kang Liao, Qing-Shan Shi, Xu-Bin Huang, Xiao-Bao Xie
Summary: Sulfur compounds found in garlic, such as diallyl trisulfide (DATS), diallyl disulfide (DADS), and diallyl sulfide (DAS), can inhibit the quorum sensing and virulence of Pseudomonas aeruginosa. Among these compounds, DATS exhibits the strongest inhibitory effects. These findings are significant for the control of P. aeruginosa virulence and pathogenicity.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2022)
Article
Biochemical Research Methods
Gang Zhou, Ying-si Wang, Hong Peng, Su-juan Li, Ting-Ii Sun, Qing-shan Shi, Jordi Garcia-Ojalvo, Xiao-bao Xie
Summary: Our study reveals that the combination of BIT and EDTA-2Na induces consistent protein expression alteration in a set of core proteins of C. werkmanii, which underlies a strong synergistic antimicrobial effect. This increases our understanding of the action modes of BIT and EDTA-2Na as well as their combinations.
JOURNAL OF PROTEOMICS
(2023)
Article
Biotechnology & Applied Microbiology
Gang Zhou, Ying-Si Wang, Hong Peng, Hui-Zhong Liu, Jin Feng, Su-Juan Li, Ting-Li Sun, Cai-Ling Li, Qing-Shan Shi, Xiao-bao Xie
Summary: This study investigated the phenotypic changes of increment ompF in C. werkmanii by knocking out the ompF gene. The results showed that increment ompF reduced the swimming ability and biofilm formation of the strain, and induced different osmotic stress responses. Moreover, OmpF was found to be involved in maltose transport and negatively regulated by MalT.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Engineering, Biomedical
Mingjie Chen, Yan Li, Huiming Liu, Dandan Zhang, Qing-Shan Shi, Xin-Qi Zhong, Yanzhu Guo, Xiao-Bao Xie
Summary: Lignin, a natural aromatic polymer, has various biological activities and can be used as an environmentally friendly antimicrobial. However, its heterogeneity and ambiguous mechanism of action pose challenges in its application. This review aims to provide insights into lignin chemistry, antimicrobial activity, synthesis approaches, and applications, to inspire researchers in developing lignin antimicrobials.
MATERIALS TODAY BIO
(2023)
Article
Chemistry, Applied
Peng Dong, Qingshan Shi, Ruqun Peng, Yingzi Yuan, Xiaobao Xie
Summary: By modifying chitosan oligosaccharide (COS) with the Eschweiler-Clarke reaction, a derivative called DMCOS was synthesized. Analysis revealed that DMCOS had converted primary amines into tertiary amines after methylation. DMCOS showed less thermal stability than COS, but exhibited stronger antimicrobial activity against bacterial and yeast strains, while also displaying lower antioxidant activity and causing mitochondrial damage in C. albicans cells.
CARBOHYDRATE POLYMERS
(2023)
Article
Environmental Sciences
Ping Yang, Hui Huang, XiaoBao Xie
Summary: The spread of antibiotic resistant bacteria from wastewater to the environment poses serious threats to human health. A potential solution is to use photosensitizers with broad-spectrum antibacterial activity for photo-oxidation in wastewater treatment. In this study, an aggregation-induced emission photosensitizer, TBTPy, was successfully designed and developed. TBTPy can efficiently generate singlet oxygen under white-light irradiation, leading to rapid and effective photo-oxidative killing of bacteria and fungi.
ENVIRONMENTAL POLLUTION
(2023)
Article
Chemistry, Physical
Jiabao Luo, Ping Yang, Jingxi Cheng, Jiaqi Fan, Weiying Zhou, Yaru Lu, XiaoBao Xie, Wenbo Wu, Xinguo Zhang
Summary: This study successfully synthesized two efficient far-red/near-infrared photosensitizers with bright emission, which showed advantages in discriminating different types of bacteria and distinguishing dead and live cells, and demonstrated broad-spectrum antibacterial properties. The study also revealed the antimicrobial mechanism and showed the potential of these photosensitizers for treating infected burns in vivo.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Microbiology
Wen-Ru Li, Tao-Hua Zeng, Zhi-Qing Zhang, Qing-Shan Shi, Xiao-Bao Xie
Summary: In this study, it was found that geraniol can inhibit the growth of Pseudomonas aeruginosa, prolong the lag phase and growth period. Geraniol also suppresses the expression of the three quorum sensing systems of P. aeruginosa, thereby attenuating the virulence factors regulated by these systems. This research is significant for improving the treatment of bacterial infections caused by P. aeruginosa.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Hong Qi, Qingshan Shi, Ruqun Peng, Tingli Sun, Zheng Zhang, Liangqiu Li, Xiaobao Xie
Summary: This study isolated a strain SRB-Z from the Pearl River in Guangzhou and examined its effect and mechanisms on corrosion of Q235 carbon steel. The results indicate that SRB-Z can cause serious pitting corrosion, with a maximum pit depth of 54 μm after 7 days of immersion corrosion. SRB-Z promoted the cathodic reaction rate of Q235, and pitting corrosion occurred due to galvanic corrosion caused by the formation of an FeS-SRB/Fe galvanic couple under the synergistic effect of the SRB-Z biofilm and its metabolite (H2S) on the Q235 coupon surfaces.
Article
Nanoscience & Nanotechnology
Junwei Yao, Heng Zhi, Qingshan Shi, Yu Zhang, Jin Feng, Jingxia Liu, Hui Huang, Xiaobao Xie
Summary: This study focused on the synergistic mechanism of tannic acid (TA)-interfacial-modified pro-ethyl cellulose (EC) nanoparticles for improving the bioavailability and biosafety of prochloraz (Pro). The TA-modified Pro-EC nanoparticles exhibited superior transmembrane permeability and adherence ability to the fungal strain Fusarium graminearum, resulting in increased reactive oxygen species production and decreased membrane potential and ergosterol content. Additionally, the toxicity of the TA-modified nanoparticles to adult zebrafish was significantly reduced compared to raw Pro. These findings suggest that the interfacial modification of Pro-EC nanoparticles with TA is a promising and environmentally safe approach for sustainable agricultural applications.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Microbiology
Gang Zhou, Lei Gao, Bao-Zhu Fang, Ying-Si Wang, Hong-Bing Tao, Xia Wen, Qian Wang, Xiao-Mo Huang, Qing-Shan Shi, Wen-Jun Li, Xiao-Bao Xie
Summary: A new Gram-positive bacterium, Fundicoccus culcitae sp. nov., was isolated from a spoiled eye mask in Guangdong, China. It showed distinct characteristics compared to other strains in the genus Fundicoccus, including growth conditions and cell composition.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yan Li, Mingjie Chen, Huiming Liu, Dandan Zhang, Qing-Shan Shi, Xiao-Bao Xie, Yanzhu Guo
Summary: This study synthesized aminoalkyl-modified lignins with different degrees of substitution and hydrophobicity by employing alkyldiamines as lysine mimics. The results showed that aminobutyl lignin had enhanced antimicrobial activity against S. aureus and E. coli and performed better than copper ions. The antimicrobial mechanism of action was similar to polylysine, damaging the cell membrane and causing cell death.
Article
Engineering, Environmental
Yinhua Cui, Qingshan Shi, Zeng Liu, Jingchun Lv, Chao Wang, Xiaobao Xie, Shaohui Zhang
Summary: In this study, a new approach is proposed to prepare flexible MXene/biomass/chitosan aerogel (MBC) electrodes with pseudo-capacitive properties through electro-static self-assembly and hydrothermal methods. The MBC-6 electrode, annealed by lyophilization, exhibits a volumetric specific capacitance of 1801.4 mF/cm3 and an ultra-high volume energy density of 33.4 Wh/L. This research presents a plausible approach for creating energy storage devices with high energy density and voltage window.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Microbiology
Gang Zhou, Qian Wang, Yingsi Wang, Xia Wen, Hong Peng, Ruqun Peng, Qingshan Shi, Xiaobao Xie, Liangqiu Li
Summary: This review examines the properties and interactions of five common outer membrane proteins (Omps), including OmpA, OmpC, OmpF, OmpW, and OmpX, in relation to porin-mediated permeability in gram-negative bacteria. It also highlights the discovered regulatory characteristics and identified molecular mechanisms in antibiotic penetration through Omps. Uncovering the functional properties of Omps will pave the way for investigating effective agents or approaches that use Omps as targets to eliminate resistant gram-negative bacteria.
Article
Biotechnology & Applied Microbiology
Shaofeng Zhou, Wenwen An, Cuifen Gan, Meiying Xu
Summary: Bacteria generate and release extracellular reactive oxygen species (eROS), which significantly contribute to the natural ROS pool and play a crucial role in metabolic interactions of bacteria with their environment. Understanding the behavior of eROS-generating bacteria and its environmental implications is of great importance in the cycling of biogeochemical elements, transformation of organic matter, and regulation of antibiotic resistance genes.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Ji-Dong Gu, Tim Ford, Bryce Mitton, Ralph Mitchell
Summary: Plastics and microplastics are widely present and accumulating in the environment, leading to increasing public attention. In addition to packaging plastics, materials from industries such as electronics, space and aviation are also accumulating, but receiving less attention. The production of degradable and biodegradable plastics is seen as a way to reduce environmental impacts, but enhancing the non-degradability of functional polymers is also a feasible option. Microbial colonization and deterioration pose significant challenges to these materials.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Justyna Bohacz, Michal Mozejko
Summary: This study evaluated the keratinolytic activity of 37 strains of Trichophyton ajelloi and found that pigmented strains isolated from loamy soil were more efficient in degrading native feather keratin, while non-pigmented strains isolated from chernozem showed better abilities. The keratinolytic activity increased over time, as evidenced by the increase in extracellular keratinase activity and release of soluble proteins and peptides. Protease activity peaked in the first 7 days and again on day 28 of culture. The release of ammonium and sulfate ions, associated with an increase in pH, reached its maximum on day 21. Three pigment-producing strains were among the most active in releasing large amounts of ammonium and sulfate ions. The biodegradation of feather waste by Trichophyton ajelloi strains can be used to produce fertilizers suitable for plants with high sulfur requirements.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Joseph M. Suflita, Brenda J. Little
Summary: This article reviews the major achievements of the International Biodeterioration & Biodegradation Society (IBBS) on its 50th anniversary and provides recommendations for addressing new challenges and developments based on personal experiences.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Zhenyan Lin, Tuo Jin, Xin Xu, Xiao Yin, Dan Zhang, Mengjiao Geng, Chunyu Pang, Gongwen Luo, Lizhi Xiong, Jianwei Peng, Jiangchi Fei
Summary: The incomplete recycle of residual mulch film (RMF) in recent years has led to an increase in plastic fragments in soil, posing a serious threat to the soil ecological environment. In this study, seven potential plastic-degrading microorganisms were screened and identified from film-mulched vegetable fields. The degradation ability experiments showed that both single strains and mixed strains demonstrated varying levels of degradation of RMF.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Tiina Belt, Michael Altgen, Muhammad Awais, Martin Nopens, Lauri Rautkari
Summary: This study investigated the degradation of heat-treated wood by brown rot fungi and found that the decay increased the hygroscopicity of wood in the decaying state. Furthermore, it was observed that heat-treated samples were more susceptible to fungal decay compared to untreated samples, which may be related to the increase in moisture content.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)
Article
Biotechnology & Applied Microbiology
Marta Cicardi, Davide Bernasconi, Luca Martire, Linda Pastero, Giulia Caneva, Sergio E. Favero-Longo
Summary: This study investigated the phenomenon of Centimetric circular areas Uncolonized by Microbial Biofilms (CUMBs) on natural and heritage stone surfaces. The analysis revealed a compatibility in distribution and size between CUMBs and lichen thalli, suggesting a lichen origin for some CUMBs. Microscopic analysis also showed similar modifications in marble layers beneath CUMBs and lichens, indicating a possible alteration of stone properties by lichens.
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
(2024)