Journal
FOOD CHEMISTRY
Volume 321, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.126708
Keywords
Paper chip; DNA extraction; Mitochondrial DNA; PCR analysis; Meat detection
Funding
- GIST (Gwangju Institute of Science and Technology), Korea, under the Practical Research and Development support program
- Bio & Medical Technology Development Program of the National Research Foundation (NRF) - Ministry of Science ICT [NRF-2015M3A9E2031375]
- Global Research Lab (GRL) Program [NRF2013K1A1A2A02050616]
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Detection of food adulteration is a challenge. However, the identification of adulterated meat in processed products is important for health and personal preference. Mitochondrial genomic DNA (mtDNA) is a good candidate for reliable identification of meat ingredients; however, the extraction of mtDNA from processed products is a bottleneck for development of detection strategies. Therefore, we constructed a rapid (similar to 5 min) mtDNA extraction device. mtDNAs from different meat samples, such as pork (Sus scrofa), chicken (Gallus gallus), and beef (Bos taurus), were successfully detected in up to 0.1% adulterated animal species. We believe that the proposed strategy could be applied to detect animal species from processed meat products to reduce fraudulent practices.
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