Spy chemistry‐enabled protein directional immobilization and protein purification

Synthesis and Fabrication of Surface-Active Microparticles Using a Membrane Emulsion Technique and Conjugation of Model Protein via Strain-Promoted Azide–Alkyne Click Chemistry in Physiological Conditions

(2019) Grace Walden et al.   BIOCONJUGATE CHEMISTRY

Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox

(2019) Irsyad N. A. Khairil Anuar et al.   Nature Communications

Oriented Immobilization of Single-Domain Antibodies Using SpyTag/SpyCatcher Yields Improved Limits of Detection

(2019) George P. Anderson et al.   ANALYTICAL CHEMISTRY

Active Template Synthesis of Protein Heterocatenanes

(2019) Xiao-Di Da et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The Therapeutic Potential of Nanobodies

(2019) Ivana Jovčevska et al.   BIODRUGS

Modular assembly of proteins on nanoparticles

(2018) Wenwei Ma et al.   Nature Communications

The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins

(2017) Erik A. Rodriguez et al.   TRENDS IN BIOCHEMICAL SCIENCES

A cleavable self-assembling tag strategy for preparing proteins and peptides with an authentic N-terminus

(2017) Qing Zhao et al.   Biotechnology Journal

Label-free quantification in ion mobility–enhanced data-independent acquisition proteomics

(2016) Ute Distler et al.   Nature Protocols

Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts

(2015) Oveimar Barbosa et al.   BIOTECHNOLOGY ADVANCES

Site-specific immobilization of protein layers on gold surfaces via orthogonal sortases

(2015) Maryam Raeeszadeh-Sarmazdeh et al.   COLLOIDS AND SURFACES B-BIOINTERFACES

SpyTag/SpyCatcher Cyclization Confers Resilience to Boiling on a Mesophilic Enzyme

(2014) Christopher Schoene et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Enzyme directed formation of un-natural side-chains for covalent surface attachment of proteins

(2014) Hwayoung Cho et al.   COLLOIDS AND SURFACES B-BIOINTERFACES

Development of VHH Antibodies against Dengue Virus Type 2 NS1 and Comparison with Monoclonal Antibodies for Use in Immunological Diagnosis

(2014) Aneela Fatima et al.   PLoS One

Synthesis of bioactive protein hydrogels by genetically encoded SpyTag-SpyCatcher chemistry

(2014) F. Sun et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

High-throughput screening for the development of a monoclonal antibody affinity precipitation step using ELP-z stimuli responsive biopolymers

(2013) Rahul D. Sheth et al.   BIOTECHNOLOGY AND BIOENGINEERING

Evaluation of immobilized enzymes for industrial applications

(2013) Andreas Liese et al.   CHEMICAL SOCIETY REVIEWS

SNAP-tag as a Tool for Surface Immobilization

(2013) Sinem Engin et al.   CURRENT PHARMACEUTICAL DESIGN

Structural Analysis and Optimization of the Covalent Association between SpyCatcher and a Peptide Tag

(2013) Long Li et al.   JOURNAL OF MOLECULAR BIOLOGY

Controlling Macromolecular Topology with Genetically Encoded SpyTag–SpyCatcher Chemistry

(2013) Wen-Bin Zhang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Site-specific chemical protein conjugation using genetically encoded aldehyde tags

(2012) David Rabuka et al.   Nature Protocols

Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin

(2012) B. Zakeri et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

NMR Spectroscopic and Theoretical Analysis of a Spontaneously Formed Lys-Asp Isopeptide Bond

(2010) Robert M. Hagan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

The potential role of self-cleaving purification tags in commercial-scale processes

(2010) Baley A. Fong et al.   TRENDS IN BIOTECHNOLOGY