Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox

Engineering a Rugged Nanoscaffold to Enhance Plug-and-Display Vaccination

(2018) Theodora Ulli Jordanka Bruun et al.   ACS Nano

Assembling and decorating hyaluronan hydrogels with twin protein superglues to mimic cell-cell interactions

(2018) Robert Wieduwild et al.   BIOMATERIALS

A novel C3d-containing oligomeric vaccine provides insight into the viability of testing human C3d-based vaccines in mice

(2018) Yong-Gang He et al.   IMMUNOBIOLOGY

SnoopLigase Catalyzes Peptide–Peptide Locking and Enables Solid-Phase Conjugate Isolation

(2018) Can M. Buldun et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Programmed loading and rapid purification of engineered bacterial microcompartment shells

(2018) Andrew Hagen et al.   Nature Communications

New Routes and Opportunities for Modular Construction of Particulate Vaccines: Stick, Click, and Glue

(2018) Karl D. Brune et al.   Frontiers in Immunology

Diffusion Measurements of Swimming Enzymes with Fluorescence Correlation Spectroscopy

(2018) Jan-Philipp Günther et al.   ACCOUNTS OF CHEMICAL RESEARCH

Self-Assembling All-Enzyme Hydrogels for Flow Biocatalysis

(2018) Theo Peschke et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Post-translational Assembly of Protein Parts into Complex Devices by Using SpyTag/SpyCatcher Protein Ligase

(2018) Ashley R. Sutherland et al.   CHEMBIOCHEM

Accelerating the clinical development of protein-based vaccines for malaria by efficient purification using a four amino acid C-terminal ‘C-tag’

(2017) Jing Jin et al.   INTERNATIONAL JOURNAL FOR PARASITOLOGY

Superior Properties of Fc-comprising scTRAIL Fusion Proteins

(2017) Meike Hutt et al.   MOLECULAR CANCER THERAPEUTICS

Single-molecule techniques in biophysics: a review of the progress in methods and applications

(2017) Helen Miller et al.   REPORTS ON PROGRESS IN PHYSICS

Two strategies to engineer flexible loops for improved enzyme thermostability

(2017) Haoran Yu et al.   Scientific Reports

Effect of His-Tag on Expression, Purification, and Structure of Zinc Finger Protein, ZNF191(243-368)

(2016) Dongxin Zhao et al.   BIOINORGANIC CHEMISTRY AND APPLICATIONS

Plug-and-playable fluorescent cell imaging modular toolkits using the bacterial superglue, SpyTag/SpyCatcher

(2016) Hyojin Moon et al.   CHEMICAL COMMUNICATIONS

RG7386, a Novel Tetravalent FAP-DR5 Antibody, Effectively Triggers FAP-Dependent, Avidity-Driven DR5 Hyperclustering and Tumor Cell Apoptosis

(2016) P. Bru nker et al.   MOLECULAR CANCER THERAPEUTICS

Programmable polyproteams built using twin peptide superglues

(2016) Gianluca Veggiani et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rapid analysis of protein expression and solubility with the SpyTag–SpyCatcher system

(2016) Dustin Dovala et al.   PROTEIN EXPRESSION AND PURIFICATION

Protein purification and crystallization artifacts: The tale usually not told

(2016) Ewa Niedzialkowska et al.   PROTEIN SCIENCE

Peptides in headlock – a novel high-affinity and versatile peptide-binding nanobody for proteomics and microscopy

(2016) Michael B. Braun et al.   Scientific Reports

Plug-and-Display: decoration of Virus-Like Particles via isopeptide bonds for modular immunization

(2016) Karl D. Brune et al.   Scientific Reports

Improving brightness and photostability of green and red fluorescent proteins for live cell imaging and FRET reporting

(2016) Bryce T. Bajar et al.   Scientific Reports

Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher

(2015) Samuel C Reddington et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Designing multivalent probes for tunable superselective targeting

(2015) Galina V. Dubacheva et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Apo2L/TRAIL and the Death Receptor 5 Agonist Antibody AMG 655 Cooperate to Promote Receptor Clustering and Antitumor Activity

(2014) Jonathan D. Graves et al.   CANCER CELL

Integrating Biological Redesign: Where Synthetic Biology Came From and Where It Needs to Go

(2014) Jeffrey C. Way et al.   CELL

SpyAvidin Hubs Enable Precise and Ultrastable Orthogonal Nanoassembly

(2014) Michael Fairhead et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Multivalent nanobodies targeting death receptor 5 elicit superior tumor cell killing through efficient caspase induction

(2014) Heather A Huet et al.   mAbs

Synthesis of bioactive protein hydrogels by genetically encoded SpyTag-SpyCatcher chemistry

(2014) F. Sun et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Double trouble-Buffer selection and His-tag presence may be responsible for nonreproducibility of biomedical experiments

(2014) Karolina A. Majorek et al.   PROTEIN SCIENCE

Computational design of water-soluble α-helical barrels

(2014) Andrew R. Thomson et al.   SCIENCE

Compartmentalization of TNF-related apoptosis-inducing ligand (TRAIL) death receptor functions: emerging role of nuclear TRAIL-R2

(2014) U Bertsch et al.   Cell Death & Disease

Programmable biofilm-based materials from engineered curli nanofibres

(2014) Peter Q. Nguyen et al.   Nature Communications

Detrimental effect of the 6 His C-terminal tag on YedY enzymatic activity and influence of the TAT signal sequence on YedY synthesis

(2013) Monique Sabaty et al.   BMC BIOCHEMISTRY

Cholesterol Loading and Ultrastable Protein Interactions Determine the Level of Tumor Marker Required for Optimal Isolation of Cancer Cells

(2013) J. Jain et al.   CANCER RESEARCH

Pentamerisation of a scFv directed against TRAIL receptor 2 increases its antitumour efficacy

(2013) Wei Wang et al.   IMMUNOLOGY AND CELL BIOLOGY

Structural Analysis and Optimization of the Covalent Association between SpyCatcher and a Peptide Tag

(2013) Long Li et al.   JOURNAL OF MOLECULAR BIOLOGY

A Set of de Novo Designed Parallel Heterodimeric Coiled Coils with Quantified Dissociation Constants in the Micromolar to Sub-nanomolar Regime

(2013) Franziska Thomas et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Controlling Macromolecular Topology with Genetically Encoded SpyTag–SpyCatcher Chemistry

(2013) Wen-Bin Zhang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Multivalent Scaffold Proteins as Superagonists of TRAIL Receptor 2-Induced Apoptosis

(2013) J. S. Swers et al.   MOLECULAR CANCER THERAPEUTICS

APG350 Induces Superior Clustering of TRAIL Receptors and Shows Therapeutic Antitumor Efficacy Independent of Cross-Linking via Fc  Receptors

(2013) C. Gieffers et al.   MOLECULAR CANCER THERAPEUTICS

Development of the Twin-Strep-tag® and its application for purification of recombinant proteins from cell culture supernatants

(2013) Thomas G.M. Schmidt et al.   PROTEIN EXPRESSION AND PURIFICATION

Several Affinity Tags Commonly Used in Chromatographic Purification

(2013) Xinyu Zhao et al.   Journal of Analytical Methods in Chemistry

Non-amyloidogenic peptide tags for the regulatable self-assembling of protein-only nanoparticles

(2012) Ugutz Unzueta et al.   BIOMATERIALS

Passive Immunoprotection of Plasmodium falciparum-Infected Mice Designates the CyRPA as Candidate Malaria Vaccine Antigen

(2012) A. M. Dreyer et al.   JOURNAL OF IMMUNOLOGY

Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin

(2012) B. Zakeri et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Basis Set of de Novo Coiled-Coil Peptide Oligomers for Rational Protein Design and Synthetic Biology

(2012) Jordan M. Fletcher et al.   ACS Synthetic Biology

An overview of enzymatic reagents for the removal of affinity tags

(2011) David S. Waugh   PROTEIN EXPRESSION AND PURIFICATION

COMBODY: one-domain antibody multimer with improved avidity

(2010) Xuekai Zhu et al.   IMMUNOLOGY AND CELL BIOLOGY

Structure and Properties of a Complex of α-Synuclein and a Single-Domain Camelid Antibody

(2010) Erwin J. De Genst et al.   JOURNAL OF MOLECULAR BIOLOGY

Increasing protein stability by improving beta-turns

(2009) Hailong Fu et al.   PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS

The Oligomerization Domain of C4-Binding Protein (C4bp) Acts as an Adjuvant, and the Fusion Protein Comprised of the 19-Kilodalton Merozoite Surface Protein 1 Fused with the Murine C4bp Domain Protects Mice against Malaria

(2008) S. A. Ogun et al.   INFECTION AND IMMUNITY