Review
Chemistry, Analytical
Yiwei Shi, Ying Cai, Yuhong Cao, Zhanying Hong, Yifeng Chai
Summary: To accelerate the discovery of anti-cancer drugs, there is an urgent need to establish an inexpensive and simple preclinical model that can simulate the tumor microenvironment and screen the drug candidates. Microfluidic chips are a promising technology due to low reagent consumption and the capability of replicating a more representative 3D cell culture.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Jin Kim, Jinyoung Kim, Hyun-Ji Park, Eun Je Jeon, Seung-Woo Cho
Summary: Stem cell mobilization is crucial for wound healing, but predicting the therapeutic efficacy of stem cell mobilizers is difficult. To overcome this, a microfluidic device was developed to replicate in vivo drug gradients and accurately evaluate the effects of Substance P (SP) on bone marrow-derived mesenchymal stem cell (BM-MSC) migration. Unlike traditional scratch models, the microfluidic chip demonstrated the differential effects of SP and PEGylated SP on BM-MSC migration, providing a more accurate assessment of drug efficacy.
KOREAN JOURNAL OF CHEMICAL ENGINEERING
(2023)
Article
Engineering, Biomedical
Qiangqiang Tang, Xiaoyu Li, Chen Lai, Lei Li, Hongkai Wu, Yingjun Wang, Xuetao Shi
Summary: In this study, a microfluidic chip using HA as a component was developed to create a highly bionic bone environment, which was found to be more conducive to cell proliferation and osteogenic differentiation compared to PDMS. The chip successfully generated a concentration gradient of model drug and determined the drug's IC50, showing great potential in high-throughput bone-related drug screening and research.
BIOACTIVE MATERIALS
(2021)
Article
Environmental Sciences
Ping Liu, Longwen Fu, Bowei Li, Mingsan Man, Yunxia Ji, Qi Kang, Xiyan Sun, Dazhong Shen, Lingxin Chen
Summary: A simple and low-cost microfluidic chip platform was developed for cultivating cells, embryos, and larvae of fish. It allows real-time observation of development and investigation of the effects of different dissolved oxygen on fish.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Review
Chemistry, Multidisciplinary
Fangjuan Zhang, Haibing Liu, Mengqi Gao, Defu Wang, Yanbing Niu, Shaofei Shen
Summary: Concentration gradient microfluidic chips have unique advantages and broad application prospects in drug screening and other fields. They can be used not only for single cell analysis and combination drug screening, but also play an important role in cell microenvironment simulation and three-dimensional (3D) cell culture.
PROGRESS IN CHEMISTRY
(2021)
Review
Engineering, Biomedical
Lingling Fang, Yu Liu, Junfeng Qiu, Weiqing Wan
Summary: This paper provides a comprehensive review of the application of three-dimensional bioprinting technology in the fabrication of tumor-on-a-chip platforms to enhance the discovery and screening of anticancer drugs. This technology can simulate the tumor microenvironment and accurately recreate the structural and physiological aspects of the tumor, thereby accelerating the progress of cancer research.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2022)
Article
Biochemistry & Molecular Biology
Roman G. Szafran, Benita Wiatrak
Summary: In this study, the generation, stability, and linearity of molecular gradient in high-throughput drug screening microfluidic assay (HTS) were thoroughly analyzed. Mathematical models were developed to predict concentration profiles, and the precision of the equations was validated with low prediction error and high correlation coefficient. The newly established mathematical relationships became part of the new HTS biochip operating protocols, contributing to improved precision and accuracy in analytical procedures.
Article
Chemistry, Analytical
Magdalena Flont, Artur Dybko, Elzbieta Jastrzebska
Summary: Recent advances in microfluidic systems have enabled the culture of complex and three-dimensional cell models, tissues, and organs, making them useful for toxicity studies and disease mimicry. This report presents a new approach to modeling skin cancer and breast cancer using microfluidic systems. The developed microsystem allows co-culture of cancer cells with non-malignant cells to mimic the cancer microenvironment, and it has been used to assess the effectiveness of photodynamic therapy in treating melanoma and chemotherapy in treating breast cancer.
Article
Chemistry, Analytical
Magdalena Flont, Artur Dybko, Elzbieta Jastrzebska
Summary: Recent advances in microfluidic systems have enabled the development of complex and three-dimensional cell, tissue, and organ models, allowing for their use in toxicity studies and disease simulation. This report presents a new approach to modeling skin cancer and breast cancer using microfluidic systems and demonstrates the functionality of the system.
Article
Biology
Madhura Chakraborty, Baishali Mukherjee, Nanditha Nalinakshan, Arikta Biswas, Rajesh Kumble Nayak, Bidisha Sinha
Summary: This study investigates the role of membrane mechanics in collective cell migration by studying membrane height fluctuations in cell sheets from fish scales. The researchers find that membrane tension is high at the cell fronts and that interactions between leader cells and non-sticky follower cells can affect cell motility and membrane mechanics. Furthermore, there is high variability in membrane fluctuation tension profiles among cells.
Review
Pharmacology & Pharmacy
Yan Liu, Qingzhen Yang, Hui Zhang, Shuang Han, Na Liu, Hui Ren, Hui Guo, Feng Xu
Summary: Cancer-on-a-chip is a promising tool for drug screening, as it faithfully recapitulates the native cancer microenvironment in a high-throughput and cost-effective manner. It simplifies the process of drug loading and cellular characterization, and is expected to promote the development of drug screening in both drug development and personal drug administration.
DRUG DISCOVERY TODAY
(2021)
Article
Biology
Ching-Te Kuo, Yu-Sheng Lai, Siang-Rong Lu, Hsinyu Lee, Hsiu-Hao Chang
Summary: Personalized drug cocktails have become popular in cancer treatment due to their use of drug combinations for better efficacy. In this study, a microcrater-arrayed (mu CA) chip was developed using laser direct writing to optimize cell amount and reagent usage. The results showed that the mu CA chip had better drug prediction accuracy compared to the conventional assay. This research has important implications for high-throughput drug screening and personalized medicine.
Review
Pharmacology & Pharmacy
Flavia Castro, Catarina Leite Pereira, Maria Helena Macedo, Andreia Almeida, Maria Jose Silveira, Sofia Dias, Ana Patricia Cardoso, Maria Jose Oliveira, Bruno Sarmento
Summary: Colorectal cancer is a heterogeneous and molecularly complex disease that requires novel therapeutic approaches. Traditional 2D models and animal models have limitations, while multicellular 3D systems are emerging as a promising tool in cancer research.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Cell Biology
Urszula Hohmann, Julian Cardinal von Widdern, Chalid Ghadban, Maria Cristina Lo Giudice, Gregoire Lemahieu, Elisabetta Ada Cavalcanti-Adam, Faramarz Dehghani, Tim Hohmann
Summary: Collective behavior of cells is crucial for wound healing, embryonic and tumor development, and can undergo a transition from a migratory unjammed state to a static jammed state depending on cell density and interactions. This study investigated the collective migration dynamics of astrocytes and glioblastoma cells and explored cell adhesion, traction, and mechanics using various techniques. Results showed that astrocytes remained in a non-migratory jammed state, while glioblastoma cells exhibited migratory unjammed behavior. Switching between jammed and unjammed states could be induced by altering the balance between cell-cell adhesion and tension via inhibition of ROCK or myosin II. These findings are important for understanding glioblastoma cell infiltration and developing novel anti-migratory drugs and treatment strategies.
Article
Computer Science, Artificial Intelligence
Youyi Song, Jing Zou, Kup-Sze Choi, Baiying Lei, Jing Qin
Summary: Cell classification is crucial for intelligent cervical cancer screening, but the variation in cells' appearance and shape poses challenges. A new learning algorithm, worse-case boosting, is proposed to improve classification accuracy for under-represented data. Experimental results demonstrate the effectiveness of this algorithm in two publicly available datasets, achieving a 4% improvement in accuracy.
MEDICAL IMAGE ANALYSIS
(2024)
Article
Biochemical Research Methods
Jisong Ahn, Jaesang Kim, Jessie S. Jeon, Young Jin Jang
Summary: Exercise plays a critical role in maintaining and improving skeletal muscle. This study developed a motion-mimicking system using a three-dimensional cellular model to analyze the changes induced by resistance exercise at both morphological and molecular levels.
Article
Chemistry, Multidisciplinary
Seunggyu Kim, Hyeono Nam, Beomseok Cha, Jinsoo Park, Hyung Jin Sung, Jessie S. Jeon
Summary: The cytotoxic response of natural killer (NK) cells in a microreactor to surface acoustic waves (SAWs) is investigated. Results show that SAWs do not damage suspended NK cells, and can increase intracellular Ca2+ concentrations and enhance cell-mediated cytotoxicity.
Article
Acoustics
Anam Bhatti, Takuro Ishii, Naoya Kanno, Hayato Ikeda, Kenichi Funamoto, Yoshifumi Saijo
Summary: Observing alterations in cutaneous vasculature can be a potential diagnostic marker for diseases. High-frequency ultrasound (HFUS) has been used in dermatology to observe skin morphologies and tissue conditions, but its ability to visualize micro-vessels is limited. In this study, a novel region-based SVD filtering approach was proposed to visualize cutaneous vascular networks. Experimental observations showed that this approach improved the image quality and has potential applications in the diagnosis of cutaneous disorders.
Article
Acoustics
Muhammad Shiddiq Sayyid Hashuro, Simon Tupin, Narendra Kurnia Putra, Kotaro Daibo, Kosuke Inoue, Takuro Ishii, Hiroyuki Kosukegawa, Kenichi Funamoto, Toshiyuki Hayase, Makoto Ohta
Summary: A transparent ultrasound flow phantom material composed of poly(vinyl alcohol) hydrogel (PVA-H) with dimethyl sulfoxide (DMSO) and water solution was proposed for visualizing and measuring flow inside ultrasound systems. The material's refractive index was modified to match that of glass for transparency. Feasibility tests were conducted, and an ultrasound flow phantom was fabricated for B-mode visualization and Doppler-PIV comparison. The results showed promising accuracy in velocity measurement and similarity to real tissue visualization, with some limitations regarding sound velocity and Doppler measurements.
ULTRASOUND IN MEDICINE AND BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Naoyuki Takahashi, Daisuke Yoshino, Ryuji Sugahara, Satomi Hirose, Kazuki Sone, Jean-Paul Rieu, Kenichi Funamoto
Summary: This study developed a microfluidic platform for reconstructing hypoxic vascular microenvironments, by simultaneously applying hypoxic stress and fluid shear stress. The results showed that under the simultaneous exposure to hypoxic stress and fluid shear stress, the migration velocity of endothelial cells increased and gradually decreased, reaching the lowest value. Meanwhile, the cells aligned and elongated in the flow direction, with enhanced expression of VE-cadherin and actin filament assembly. Therefore, this microfluidic platform is useful for investigating the dynamics of endothelial cells in vascular microenvironments.
SCIENTIFIC REPORTS
(2023)
Article
Nanoscience & Nanotechnology
Suguru Miyauchi, Koichi Hosoi, Shingo Tsuda, Toshiyuki Hayase, Kenichi Funamoto
Summary: This study investigated the effects of complex internal structures and torsional motion on left ventricular hemodynamics using computational fluid dynamics analyses. The presence of trabeculae carneae and papillary muscles decreased average wall shear stress and increased the relative residence time of blood stagnation at the apex of the left ventricle. Torsional motion caused irregular changes to the relative residence time level and distribution at the apex. Therefore, the complex internal structures and torsional motion of the left ventricle could cause blood stagnation.
Article
Cell Biology
Satomi Hirose, Julie Hesnard, Nasser Ghazi, Damien Roussel, Yann Voituron, Oliver Cochet-Escartin, Jean-Paul Rieu, Christophe Anjard, Kenichi Funamoto
Summary: Spatial and temporal variations of oxygen environments affect cell behaviors, and the aerotaxis of Dictyostelium discoideum seems to be an effective strategy for survival. Flavohemoglobins, potential oxygen sensors and oxidative stress modulators, play a role in this phenomenon.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Engineering, Biomedical
Seunggyu Kim, Joonha Park, Jin-Nyoung Ho, Danhyo Kim, Sangchul Lee, Jessie S. Jeon
Summary: Despite the advantages of microfluidic system, vascular systems for drug transport have been overlooked in microfluidic-based chemotherapeutic screening. In this study, we investigated the chemotherapeutic response of bladder tumor cells using a vascularized tumor on a chip. The chip allowed the establishment of tumor-hydrogel-endothelium interfaces and evaluation of drug resistance and efficacy. Our findings suggest that the vascularized tumor chip enables investigations of chemotherapeutic screening.
Article
Materials Science, Biomaterials
Ungsig Nam, Seokhun Lee, Jessie S. Jeon
Summary: The research team has successfully developed a microphysiological system-based model of the outer blood-retinal barrier (oBRB) and choriocapillaris. This model can simulate the pathological changes caused by diseases like diabetes in the retina and choroid, providing a valuable in vitro platform for preclinical applications.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Article
Engineering, Biomedical
Shuhei Murase, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino, Kouji Takano, Ayumu Konno, Hirokazu Hirai, Taku Saito, Sakae Tanaka, Keisuke Shinohara, Takuya Kishi, Yuki Yoshikawa, Takamasa Sakai, Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Atsushi Takashima, Toru Ogata, Hirotsugu Tsuchimochi, Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Yuiko Matsuura, Kenichi Funamoto, Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao, Yasuhiro Sawada
Summary: Vertically oscillating head motions can reduce blood pressure in hypertensive individuals, possibly by reducing the expression of angiotensin II type-1 receptor in astrocytes. This suggests that mechanical interventions could be used to elicit antihypertensive effects.
NATURE BIOMEDICAL ENGINEERING
(2023)
Meeting Abstract
Peripheral Vascular Disease
Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino, Kouji Takano, Keisuke Shinohara, Takuya Kishi, Koji Tomiyasu, Toru Ogata, Hirotsugu Tsuchimochi, Shigeyoshi Saito, Kohzoh Yoshino, Kenichi Funamoto, Masahiro Shinohara, Motoshi Nagao, Yasuhiro Sawada
JOURNAL OF HYPERTENSION
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
Yasuhiro Sawada, Naoyoshi Sakitani, Takahiro Maekawa, Daisuke Yoshino, Kouji Takano, Keisuke Shinohara, Takuya Kishi, Ayumu Konno, Hirokazu Hirai, Makoto Ayaori, Hirohiko Inanami, Koji Tomiyasu, Toru Ogata, Hirotsugu Tsuchimochi, Shinya Sato, Shigeyoshi Saito, Kohzoh Yoshino, Kenichi Funamoto, Hiroki Ochi, Masahiro Shinohara, Motoshi Nagao
Review
Oncology
Seunggyu Kim, Zhengpeng Wan, Jessie S. Jeon, Roger D. Kamm
Summary: Emerging microfluidic disease models have been widely used in cancer research to replicate the process of tumor cell metastasis. By capturing key features of microvasculature and investigating dynamic extravasation behaviors, microfluidic systems contribute to the development of in vitro disease models, enhancing the understanding of metastasis in vivo.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemical Research Methods
Seokhun Lee, Seunggyu Kim, Jessie S. Jeon
Summary: In this study, an enhanced outer blood-retinal barrier (oBRB) model was proposed, with fully vascularized choriocapillaris endothelium (CCE) and an in vivo analogous distance between the retinal pigment epithelium (RPE) and CCE. The model, constructed using physiological intercellular distance, can mimic the pathogenesis of wet age-related macular degeneration (AMD) and facilitate AMD research.
Article
Biochemical Research Methods
Joonha Park, Seunggyu Kim, Jiman Hong, Jessie S. Jeon
Summary: In this study, perfusable tumor tissue was engineered by implanting multicellular tumor spheroids inside microfluidic devices, allowing monitoring of blood perfusion, spheroid growth, and vascular dynamics. Increased penetration depth of fluorescence conjugated anti-cancer drug was observed in tri-culture spheroids, demonstrating the potential applications of tumor microenvironment reconstruction.