Article
Agriculture, Multidisciplinary
Xin-Yun Wang, Jun Yan, Jing Xie
Summary: Spoilage targets of Shewanella putrefaciens XY07 were determined by genome sequencing, metabolomics, and FTIR analysis. Metabolomics analysis revealed the importance of amino acid degradation in S. putrefaciens XY07.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Agriculture, Multidisciplinary
William Chih-Wei Chang, Hsin-Yi Wu, Hung-Lin Kan, Ying-Chi Lin, Pei-Jane Tsai, Yun-Chieh Chen, Yu-Yi Pan, Pao-Chi Liao
Summary: This study aimed to identify novel spoilage markers in chicken eggs using foodomics strategies. The analysis revealed changes in specific metabolites in stored eggs, with a high predictive accuracy of 0.956. These findings may contribute to enhancing quality assurance and food safety in poultry egg production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Food Science & Technology
Jin-Wei Zhou, Peng-Cheng Ji, Chang-Yao Wang, Yu-Jie Yang, Xin-Yu Zhao, Hao-Zhe Tang
Summary: The anti-virulence potential and mechanism of Perilla frutescens-derived dihydrocuminyl aldehyde (DCA) against Pseudomonas aeruginosa XZ01 were investigated using transcriptome sequencing and metabolomics. DCA effectively reduced the production of virulence factors and disrupted biofilm formation. Mechanistically, DCA down-regulated quorum sensing-related genes, increased membrane permeability, and suppressed efflux systems, leading to heightened susceptibility to nisin and metabolic disorders.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Food Science & Technology
Ourania Misiou, Konstantinos Koutsoumanis
Summary: This paper provides an overview of the potential impact of climate change on food safety and microbial spoilage. Global warming is projected to increase the risk of microbial spoilage in food, such as contamination by mycotoxins and marine biotoxins, as well as environmental residuals and zoonotic diseases. The paper emphasizes the need for multidisciplinary approaches to study the potential effects of climate change on food and highlights the importance of preparedness by the food industry and policy makers.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Dibyajit Lahiri, Moupriya Nag, Bandita Dutta, Tanmay Sarkar, Siddhartha Pati, Debarati Basu, Zulhisyam Abdul Kari, Lee Seong Wei, Slim Smaoui, Khang Wen Goh, Rina Rani Ray
Summary: The call to cater for the hungry is a worldwide problem in the 21st century. Food security is the utmost prime factor for the increasing demand for food. Awareness of human health when using chemical preservatives in food has increased, resulting in the use of alternative strategies for preserving food and enhancing its shelf-life. Bacteriocins, particularly those produced by lactic acid bacteria (LAB), have gained importance for their antibacterial properties and their association with food fermentation. Circular bacteriocins, with their stable circular structure, have been less studied but offer potential applications in the food industry for extending shelf-life.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Chemistry, Analytical
Helena Vasconcelos, Luis C. C. Coelho, Ana Matias, Cristina Saraiva, Pedro A. S. Jorge, Jose M. M. M. de Almeida
Summary: Biogenic amines (BAs) are well-known biomolecules mainly for their toxic and carcinogenic effects. They are used as indicators of quality preservation in food and beverages, and are mainly formed by microbial decarboxylation of amino acids. The extensive and significant research on biorecognition elements, transducers, and their integration in biosensors has been reviewed over the years.
Article
Biochemistry & Molecular Biology
Minnie Jacob, Refat M. Nimer, Mohamad S. Alabdaljabar, Essa M. Sabi, Mysoon M. Al-Ansari, Maged Housien, Khalid M. Sumaily, Lina A. Dahabiyeh, Anas M. Abdel Rahman
Summary: This study used liquid chromatography-mass spectrometry (LC-MS) metabolomics to analyze the metabolome of serum from patients with nephrotic syndrome (NS) and found significant changes in 176 metabolites compared to the control group. Dysregulation of arginine, proline, and tryptophan metabolism, as well as arginine, phenylalanine, tyrosine, and tryptophan biosynthesis, were the most common metabolic pathways affected in NS.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Food Science & Technology
Trond Lovdal, Bjorn Tore Lunestad, Mette Myrmel, Jan Thomas Rosnes, Dagbjorn Skipnes
Summary: Seaweeds have a long history in the human diet in Asia and are becoming more popular in the western world. Edible seaweeds include various species of green, red, and brown macroalgae, which can be consumed fresh or processed. Ensuring microbiological food safety and quality of seaweed products is crucial, along with addressing the emerging trends and challenges in the industry such as antimicrobial resistance and legislation and guidelines.
Review
Food Science & Technology
Giuseppina Sequino, Vincenzo Valentino, Francesco Villani, Francesca De Filippis
Summary: The diffusion of high-throughput sequencing has significantly impacted the study of food microbial ecology, allowing for a better understanding of microbial communities through amplicon-based and shotgun metagenomics approaches. Moreover, retrieving Metagenome-Assembled Genomes (MAGs) enables strain-tracking and response to food poisoning outbreaks.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
V. Radhalakshmi, Maya Raman, Minnu Rose Joy
Summary: Active packaging films based on poly (lactic acid) (PLA) were developed by incorporating betel leaf ethanolic extract (BLEE) at different concentrations (5 wt% and 10 wt%). The BLEE extract exhibited excellent antioxidant and antimicrobial properties. The films' characteristics were analyzed, and it was found that the addition of BLEE reduced water solubility and water permeability, while the tensile strength showed an inverse relationship with concentration. The PLA-BLEE films successfully reduced lipid oxidation in tuna meat and exhibited potential for extending shelf life.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Pharmacology & Pharmacy
Aarzoo Thakur, Vijaya Saradhi Mettu, Dilip K. Singh, Bhagwat Prasad
Summary: Transporter-mediated drug-drug interactions (DDIs) are evaluated during drug development using probe drugs and in vitro and in vivo models. This study focuses on the potential of endogenous metabolites as transporter biomarkers for predicting DDIs in rats. The results suggest that pyridoxic acid and kynurenic acid, as potential biomarkers for DDIs mediated by organic anion transporters (OAT1 and OAT3), have limited utility in rats. Untargeted metabolomics analysis identified 21 putative biomarkers belonging to various metabolic pathways, which can be used to predict DDIs caused by OATs in rats.
BIOCHEMICAL PHARMACOLOGY
(2023)
Article
Oncology
David A. Drew, Samuel J. Klempner, Andrew T. Chan
Summary: Early detection and interception of gastric cancer globally is limited, especially for individuals without established hereditary risk factors. Establishing biomarkers using multiple-omic approaches may be crucial for identifying individuals at risk and for more intensive evaluation. Multifactorial risk assessment is needed for precision prevention and early detection of gastric cancer.
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
(2021)
Review
Endocrinology & Metabolism
Xiaohui Du, Le Yang, Ling Kong, Ye Sun, Kunshuang Shen, Ying Cai, Hui Sun, Bo Zhang, Sifan Guo, Aihua Zhang, Xijun Wang
Summary: This article reviews the application of metabolomics in diabetic retinopathy (DR) and introduces potential biomarkers such as alanine, lactate, and glutamine that may be related to the pathogenesis of DR.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Food Science & Technology
Melisa Lamri, Tanima Bhattacharya, Fatma Boukid, Imene Chentir, Amira Leila Dib, Debashrita Das, Djamel Djenane, Mohammed Gagaoua
Summary: Nanoparticles have the potential to improve the quality, shelf-life, and nutritional aspects of meat products; however, ensuring product safety, quality, and reducing costs and wastage remain key challenges that require further research.
Review
Nutrition & Dietetics
Eriko Shibutami, Toru Takebayashi
Summary: Nutrimetabolomics is an emerging field in nutritional research that aims to understand the interaction between diet and health. Through the development of omics technology, it has identified dietary biomarkers and deepened the understanding of metabolic dynamics. However, further efforts are needed to validate these biomarkers for clinical applications.
Article
Chemistry, Analytical
Hsin-Yi Wu, Mi-Chia Ma, Yu-Yi Pan, Chia-Lung Shih, Victor Zgoda, Chin-Shang Li, Lung-Cheng Lin, Pao-Chi Liao
ANALYTICAL CHEMISTRY
(2019)
Article
Biochemical Research Methods
Hsin-Yi Wu, Yu-Yi Pan, Arthur T. Kopylov, Victor Zgoda, Mi-Chia Ma, Ching-Hsun Wang, Wu-Chou Su, Wu-Wei Lai, Pin-Nan Cheng, Pao-Chi Liao
PROTEOMICS CLINICAL APPLICATIONS
(2020)
Article
Oncology
Ming-Hui Yang, Marcelo Chen, Hsiao-Hsuan Mo, Wan-Chi Tsai, Yu-Chi Chang, Chin-Chuan Chang, Ko-Chin Chen, Hsin-Yi Wu, Cheng-Hui Yuan, Che-Hsin Lee, Yi-Ming Arthur Chen, Yu-Chang Tyan
Article
Agriculture, Multidisciplinary
William Chih-Wei Chang, Hsin-Yi Wu, Hung-Lin Kan, Ying-Chi Lin, Pei-Jane Tsai, Yun-Chieh Chen, Yu-Yi Pan, Pao-Chi Liao
Summary: This study aimed to identify novel spoilage markers in chicken eggs using foodomics strategies. The analysis revealed changes in specific metabolites in stored eggs, with a high predictive accuracy of 0.956. These findings may contribute to enhancing quality assurance and food safety in poultry egg production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yuan-Chih Chen, Hsin-Yi Wu, Chih-Wei Chang, Pao-Chi Liao
Summary: Conjugation reactions play a critical role in human metabolism, but identifying conjugated metabolites is challenging. In this study, a strategy called PDMS2E-DIA was proposed for comprehensive profiling of the glucuronide-conjugated metabolome. It successfully identified a large number of glucuronide-conjugated metabolites in human urine samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Oncology
Jung-Lin Wu, Hsin-Yi Wu, Shang-Ju Wu, Ho-Yang Tsai, Shao-Hsing Weng, Kuen-Tyng Lin, Liang-In Lin, Chi-Yuan Yao, Margarita Zamanova, Yi-Yuan Lee, Takashi Angata, Hwei-Fang Tien, Yu-Ju Chen, Kuo- Lin
Summary: This study provides an understanding of the dysregulated phosphoproteome in chronic lymphocytic leukemia (CLL) and its alterations during treatment with the B-cell receptor (BCR) pathway inhibitor ibrutinib. It was found that several signaling pathways, including the BCR pathway, showed differential phosphorylation events in CLL cells. Furthermore, the study identified a constitutively elevated level of KAP1 phosphorylation in CLL samples, which was abolished by ibrutinib treatment. The study also revealed the dynamic changes of molecules associated with BCR signaling during ibrutinib treatment.
MOLECULAR CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Rashmi Madda, Chao-Ming Chen, Cheng-Fong Chen, Jir-You Wang, Hsin-Yi Wu, Po-Kuei Wu, Wei-Ming Chen
Summary: Biological reconstruction techniques have greatly improved the treatment of high-grade osteosarcoma patients by using liquid nitrogen and irradiation processes to remove tumors and reconstruct autografts. This proteomic study focuses on the expressions of growth factor family proteins in untreated and treated autografts, which play a crucial role in bone union, remodeling, and regeneration. The study found that these protein expressions were significantly higher in treated autografts, suggesting that the increased growth factor protein expression may contribute to bone regeneration healing and reduce the recurrence and spread of high-grade sarcoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Tzu-Ting Kuo, Li-Chun Lin, Hsin-Yi Chang, Pei-Jung Chiang, Hsin-Yi Wu, Tai-Yuan Chen, Shih-Min Hsia, Tsui-Chin Huang
Summary: Hot water extract of Melissa officinalis exhibits anticancer effects in colon cancer cells by modulating mitochondrial respiratory electron transport and oxidative stress response.
Article
Chemistry, Analytical
Yuan-Chih Chen, Hsin-Yi Wu, Wei-Sheng Wu, Jen-Yi Hsu, Chih-Wei Chang, Yuan-Han Lee, Pao-Chi Liao
Summary: The identification of xenobiotic biotransformation products is important for understanding their toxicity and establishing monitoring systems for public health. However, the lack of reference standards and spectral data leads to the generation of multiple candidate structures during identification, reducing the confidence in the results. This study proposes a metabolomics strategy integrated with FragAssembler, a metabolite structure elucidation approach, to reduce the number of false-positive structure candidates. The feasibility of this approach is demonstrated by the identification of biotransformation products of two xenobiotics. The results show that FragAssembler effectively eliminates false-positive reactions and chemical structures, improving the efficiency and reliability of identifying xenobiotic biotransformation products.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)