Review
Biochemistry & Molecular Biology
Marion Turpin, Gilles Salbert
Summary: DNA methylation is a highly dynamic process regulated by the cooperative actions of DNMT and TET enzymes, influenced by factors such as chromatin environment and protein partners. Mathematical models can help explain variable methylation levels during development and reveal cell-type epigenetic heterogeneity. These discoveries hold significant implications for disease treatment and the development of epigenetic drugs.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Sharna J. Coker, Carlos C. Smith-Diaz, Rebecca M. Dyson, Margreet C. M. Vissers, Mary J. Berry
Summary: The maternal diet during pregnancy has a significant impact on offspring health, with poor nutrition increasing the risk of chronic conditions and neurodevelopmental disorders. Disturbed epigenetic programming during early development is believed to be one potential biological mechanism. Vitamin C plays a critical role in embryonic development, with enzymes involved in epigenetic regulation requiring it for optimal activity. Insufficient vitamin C during pregnancy can have negative effects on neurodevelopment and epigenetic status.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biotechnology & Applied Microbiology
Hao Lv, Fu-Ying Dao, Dan Zhang, Hui Yang, Hao Lin
Summary: DNA modification is crucial in regulating gene expression in cell development, with advances in DNA sequencing technology allowing for the resolution of different modifications at a genome-wide scale. This has led to the discovery of new insights into the complexity and functions of multiple methylations. The review discusses various mapping approaches and the development of future sequencing technologies for improving detection resolution.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Multidisciplinary Sciences
Bo He, Chao Zhang, Xiaoxue Zhang, Yu Fan, Hu Zeng, Jun'e Liu, Haowei Meng, Dongsheng Bai, Jinying Peng, Qian Zhang, Wei Tao, Chengqi Yi
Summary: 5hmC in human tissues shows tissue-specific patterns that may affect tissue-specific gene expression networks and are associated with specific single-nucleotide polymorphisms and diseases.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Yang Feng, Juan-Juan Chen, Neng-Bin Xie, Jiang-Hui Ding, Xue-Jiao You, Wan-Bing Tao, Xiaoxue Zhang, Chengqi Yi, Xiang Zhou, Bi-Feng Yuan, Yu-Qi Feng
Summary: DNA cytosine methylation, specifically 5mC, is a crucial epigenetic process in higher eukaryotes, regulated by DNMTs, TET, and TDG. Active demethylation of 5mC can occur through the TET-TDG-BER pathway or direct decarboxylation of 5caC, both of which may impact genome integrity.
Article
Biology
Audrey Laurent, Thierry Madigou, Maud Bizot, Marion Turpin, Gaelle Palierne, Elise Mahe, Sarah Guimard, Raphael Metivier, Stephane Avner, Christine Le Peron, Gilles Salbert
Summary: Methylation and demethylation of cytosines in DNA play a crucial role in cell-specific gene expression. By enforcing the expression of the catalytic domain of the methylcytosine dioxygenase TET2 in breast cancer cells, researchers were able to alter the cancer-specific transcription program. TET2 decreased the tumorigenic potential of cancer cells by activating and repressing a panel of genes, leading to an exacerbation of sensitivity to autophagy inducers due to the establishment of a lysosomal storage disease-like state.
LIFE SCIENCE ALLIANCE
(2022)
Article
Chemistry, Multidisciplinary
Neng-Bin Xie, Min Wang, Tong-Tong Ji, Xia Guo, Jiang-Hui Ding, Bi-Feng Yuan, Yu-Qi Feng
Summary: The study presents an engineered deaminase-mediated sequencing (EDM-seq) method for the quantitative detection of 5hmC in DNA at single-nucleotide resolution. The method uses an engineered A3A protein to deaminate C and 5mC, while leaving 5hmC unaffected, allowing for the direct and quantitative detection of 5hmC in DNA.
Article
Multidisciplinary Sciences
Tuvshintugs Baljinnyam, Mark L. Sowers, Chia Wei Hsu, James W. Conrad, Jason L. Herring, Linda C. Hackfeld, Lawrence C. Sowers
Summary: DNA damage caused by internal reactions can lead to tumor development, and enzymatic modification of DNA bases can modulate epigenetic control of gene transcription. This study reveals the role of human glycosylases in DNA repair and epigenetic reprogramming, introducing new observations and raising new questions.
Article
Chemistry, Analytical
Jing Tang, Guangrong Zou, Chen Chen, Jing Ren, Fang Wang, Zilin Chen
Summary: This study introduced a novel electrochemical method for the selective detection of formylpyrimidines in DNA, specifically targeting 5-formyluracil (5fU) in the presence of 5-formylcytosine (5fC) and apyrimidinic (AP) sites. By combining molecule-depended chemical recognition and electrochemical sensing, the approach showed promise in sensitive and accurate detection of 5fU, with potential applications in various fields.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Florian Schelter, Angie Kirchner, Franziska R. Traube, Markus Mueller, Wolfgang Steglich, Thomas Carell
Summary: The four non-canonical nucleotides in the human genome, along with oxidized nucleotides formed by Tet enzymes, contribute to epigenetic regulation of gene expression. Experimental evidence for the presence of these oxidized nucleotides in fungi and plants is unclear, necessitating further research for clarification in these organisms.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Vanessa Ortiz-Barahona, Marta Soler, Veronica Davalos, Carlos A. A. Garcia-Prieto, Maxime Janin, Fernando Setien, Irene Fernandez-Rebollo, Joan J. J. Bech-Serra, Carolina De La Torre, Sonia Guil, Alberto Villanueva, Pei-Hong Zhang, Li Yang, Marco Guarnacci, Ulrike Schumann, Thomas Preiss, Ugne Balaseviciute, Robert Montal, Josep M. M. Llovet, Manel Esteller
Summary: This study found that the 5-methylcytosine RNA methyltransferase NSUN7 undergoes epigenetic inactivation in liver cancer, leading to abnormal mRNA methylation and transcriptional silencing. This is associated with clinical outcome and therapeutic vulnerability.
Article
Chemistry, Organic
Chenhao Lou, Xin Wang, Leiyang Lv, Zhiping Li
Summary: The iron-catalyzed three-component reaction of cyclopropanols with alkenes and tert-butyl hydroperoxide enables the efficient synthesis of 5-oxo peroxides, allowing for the modification of biologically active molecules and various downstream derivatizations.
Article
Chemistry, Organic
Xiao-Jun Deng, Hui-Xia Liu, Lu-Wen Zhang, Guan-Yu Zhang, Zhi-Xiang Yu, Wei He
Summary: This study describes a room-temperature metal-free iodoarene-catalyzed oxyamination of unactivated alkenes, resulting in the difunctionalization of alkenes by oxygen and nitrogen, leading to the synthesis of important 5-imino-2-tetrahydrofuranyl methanamine derivatives for drug development and biological studies. The mechanism involves activation of substrate alkene by a cationic iodonium(III) intermediate, followed by attack of oxygen atom from amides to form a five-membered ring intermediate, which then undergoes an S(N)2 reaction by NTs2 to give the final product. Promising results were also obtained for some substrates using chiral iodoarenes as catalysts in an asymmetric variant of this alkene oxyamination.
JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Engineering, Chemical
Jose L. Gonzalez-Alfonso, Ana Poveda, Miguel Arribas, Yoshihiko Hirose, Maria Fernandez-Lobato, Antonio Olmo Ballesteros, Jesus Jimenez-Barbero, Francisco J. Plou
Summary: By utilizing CGTase as a catalyst, rutin was polyglucosylated to enhance aqueous solubility, achieving high conversion yield under optimal conditions. The glucosylation reaction resulted in enhanced solubility and the yield of monoglucosylated product was increased with the treatment of amyloglucosidase STA1.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2021)
Article
Agricultural Engineering
Chunxiang Chen, Dening Wei, Jian Zhao, Xiaodong Huang, Dianzhan Fan, Qianhao Qi, Yingxing Bi, Liping Liao
Summary: The study investigated the weight loss characteristics, product distribution, and bio-oil composition of co-pyrolysis of rice straw and Chlorella vulgaris using microwave oven. The catalytic effects of activated carbon and HZSM-5 were also studied. The results showed that activated carbon significantly improved the weight loss characteristics, while HZSM-5 had a positive effect only at low addition levels. Compound additives increased the bio-oil production and hydrocarbons content.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Vorasit Vongsutilers, Daniel J. Phillips, Brian C. Train, Gregory R. McKelvey, Nissa M. Thomsen, Kevin H. Shaughnessy, James P. Lewis, Peter M. Gannett
BIOPHYSICAL CHEMISTRY
(2011)
Article
Chemistry, Medicinal
Brian C. Train, Suzan A. Bilgesue, Emily C. Despeaux, Vorasit Vongsutilers, Peter M. Gannett
CHEMICAL RESEARCH IN TOXICOLOGY
(2014)
Article
Biotechnology & Applied Microbiology
Nissa M. Thomsen, Vorasit Vongsutilers, Peter M. Gannett
CRITICAL REVIEWS IN EUKARYOTIC GENE EXPRESSION
(2011)
Article
Biochemistry & Molecular Biology
Vorasit Vongsutilers, Jonathan R. Daft, Kevin H. Shaughnessy, Peter M. Gannett
Article
Biochemistry & Molecular Biology
Vorasit Vongsutilers, Kulwadee Sawaspaiboontawee, Bodin Tuesuwan, Yoko Shinohara, Gota Kawai
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
(2018)
Article
Multidisciplinary Sciences
Vorapot Sapsirisavat, Vorasit Vongsutilers, Narukjaporn Thammajaruk, Kanitta Pussadee, Prakit Riyaten, Stephen Kerr, Anchalee Avihingsanon, Praphan Phanuphak, Kiat Ruxrungtham
Review
Chemistry, Medicinal
Bodin Tuesuwan, Vorasit Vongsutilers
Summary: Nitrosamine contamination in medicines has raised safety concerns, leading to stringent guidelines by regulatory authorities. Key to controlling nitrosamine issues is understanding toxicity, potential root causes, risk assessment, and suitable analytical techniques.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Chemistry, Medicinal
Bodin Tuesuwan, Wunlapa Mueannoom, Promporn Jamnongtanachot, Ariya Khunvichai, Chiravi Pavitrapok, Kawinthida Wongpakdee, Supasil Sra-ium, Nithi Mahanonda, Vorasit Vongsutilers
Summary: The formulation of favipiravir as a child-friendly dosage form has improved convenience and safety for pediatric patients in Thailand. With the use of pH control and poloxamer as a solubilizing agent, a 200 mg/15 mL oral solution was successfully manufactured and demonstrated stability at 30 degrees C for 30 days and at 5 degrees C for 60 days.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Review
Chemistry, Medicinal
Bodin Tuesuwan, Vorasit Vongsutilers
Summary: The current global situation of nitrosamine contamination has expanded to a wide range of medicines due to the possible risk of contamination through various aspects of the drug manufacturing process. Understanding the chemistry, toxicology, and root causes of nitrosamines is crucial for effectively evaluating and mitigating the associated risks. To address the risk of nitrosamine contamination in pharmaceuticals, significant knowledge and resources are required, including reliable laboratory facilities, reference materials, specific instrumentation, data management, and time. Supporting tools such as shared databases, screening models, and in silico models have proven beneficial in managing the risk of nitrosamine contamination.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)