Review
Plant Sciences
Cecilia Rodriguez-Furlan, Rita Borna, Oliver Betz
Summary: This article summarizes the functions of plant RAB7 and its role in endomembrane trafficking, including endocytic traffic, biosynthetic traffic, recycling from late endosome to the secretory pathway, and autophagic trafficking. It also highlights unresolved challenges in the field and recommends future research directions.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Qiaoqiao Lv, Xifeng Li, Baofang Fan, Cheng Zhu, Zhixiang Chen
Summary: Glucosinolates are important secondary metabolites in Brassicales plants, playing a critical role in chemical defense. They are chemically inactive but can be hydrolyzed by myrosinases to produce toxic compounds, forming the glucosinolate-myrosinase defense system. Research has revealed that Brassicales plants have complex biosynthetic pathways for glucosinolate production and different classes of myrosinases with distinct catalytic mechanisms and substrate specificity. Progress has been made in understanding the cellular and subcellular organization of the glucosinolate-myrosinase system, especially in the detonation of the mustard oil bomb after tissue damage caused by herbivores and pathogens.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Plant Sciences
Jian Ye, Lili Zhang, Xuan Zhang, Xiujuan Wu, Rongxiang Fang
Summary: Plants face dual damage when infected with insect-borne microbial pathogens. Research has shown that many host genes involved in multistress resistance are related to innate immunity and plant hormone signaling, especially jasmonate and salicylic acid. Utilizing genome editing or chemical modulators to fine-tune crop defensive signaling offers potential for sustainable control of insect-borne diseases.
TRENDS IN PLANT SCIENCE
(2021)
Review
Plant Sciences
Ivana Jovanovic, Nicole Frantova, Jan Zouhar
Summary: The plant endomembrane system plays a crucial role in plant growth and development, as well as in responses to abiotic and biotic stresses. Plant viruses remodel this system to create replication compartments and evade host defenses. Recent research has revealed that plant antiviral immunity involves receptor-like kinases and selective autophagy, highlighting the complex interplay between viral components and the plant endomembrane-associated pathways.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Yosia Mugume, Rahul Roy, William Agbemafle, Gabriella N. Shepard, Yee Vue, Diane C. Bassham
Summary: It is found that Arabidopsis VPS45 interacts with the SYP61/SYP41/VTI12 SNARE complex and plays crucial roles in the stability of SYP41, cargo trafficking, and cell expansion. Mutant vps45-3, with a point mutation causing a serine-to-phenylalanine substitution, exhibits severe growth defects, including reduced organ and cell size, compromised tip growth of root hair and pollen tube, as well as vacuolar defects. Loss of turgor pressure due to vacuolar defects is proposed as the underlying cause of the observed growth defects.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Plant Sciences
Huijia Kang, Tianyi Fan, Jiabing Wu, Yan Zhu, Wen-Hui Shen
Summary: Plants, as sessile organisms, are constantly exposed to diverse stresses, including pathogen invasion. Recent studies have found that pathogen infections can induce epigenetic changes in plants, leading to the reprogramming of plant defense genes. These mechanisms could be used in the design of stress-resistant plant varieties.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Miguel Sampaio, Joao Neves, Tatiana Cardoso, Jose Pissarra, Susana Pereira, Claudia Pereira
Summary: Plant cells have developed various mechanisms to cope with stress and changes in their environment, including alterations in protein trafficking and morphology of key organelles. While the understanding of these processes is still limited, recent research is shedding light on the complexity and dynamics of protein transport routes and their significance in maintaining cell homeostasis under harsh conditions.
Review
Biochemistry & Molecular Biology
Yulong Ren, Yongfei Wang, Yu Zhang, Tian Pan, Erchao Duan, Xiuhao Bao, Jianping Zhu, Xuan Teng, Pengcheng Zhang, Chuanwei Gu, Hui Dong, Fan Wang, Yunlong Wang, Yiqun Bao, Yihua Wang, Jianmin Wan
Summary: Seed storage proteins (SSPs) are the major protein nutrition sources for human and livestock, and they are transported through a plant-specific endomembrane system. Recent studies have uncovered the complex network controlling SSP trafficking, involving both evolutionarily conserved molecular machineries and plant-unique regulators. This review summarizes the current knowledge of protein body biogenesis and endomembrane-mediated SSP transport, and discusses cutting-edge strategies for advancing the field.
Review
Plant Sciences
Qian Ma, Mingqin Chang, Georgia Drakakaki, Eugenia Russinova
Summary: The endomembrane system is crucial for plant growth and development. Small-molecule compounds have been used as powerful tools to study membrane trafficking in plant cells, but more precise chemical probes are needed for a better understanding of the complex trafficking processes. Various chemoproteomic approaches can help determine the mechanism of action of compounds and improve their specificity. Rational molecule design, virtual screening, and artificial intelligence can aid in the discovery of highly precise chemical probes more efficiently. In the next decade, more accurate tools and advanced live quantitative imaging techniques will deepen our insights into the plant endomembrane system.
CURRENT OPINION IN PLANT BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mikhail Rudinskiy, Maurizio Molinari
Summary: Conserved catabolic pathways operate to remove aberrant polypeptides from the endoplasmic reticulum (ER), utilizing ERAD pathways and ER-phagy. ERAD pathways control retrotranslocation of misfolded proteins across the ER membrane, while ER-phagy segregates proteins into ER subdomains that eventually vesiculate. These ER-derived vesicles can be captured by autophagosomes, endolysosomes/vacuoles, or fused with degradative organelles.
Review
Microbiology
Marzieh Ebrahimi-Zarandi, Roohallah Saberi Riseh, Mika T. Tarkka
Summary: Pathogen suppression and induced systemic resistance are alternative strategies for sustainable agriculture, and Actinobacteria have been widely used as biocontrol agents. Actinobacteria not only produce substances and enzymes that antagonize pests, but also stimulate plant growth. They can also induce systemic resistance against pathogens, but the mechanisms are still unclear.
Review
Biochemistry & Molecular Biology
Qian Liu, Chunhong Zhang, Huiyong Fang, Letai Yi, Minhui Li
Summary: In a complex natural environment, plants have developed complex defense response mechanisms, including the NBS-LRR protein and alkaloids, to protect themselves. The NBS-LRR protein recognizes pathogenic microorganisms and triggers the immune response, while alkaloids inhibit pathogens. This study reviews the activation, recognition, and signal transduction of NBS-LRR proteins, as well as the synthesis and regulation of alkaloids in plant protection and discusses their current and future applications in biotechnology.
Article
Multidisciplinary Sciences
Rene Glenz, Agnes Kaiping, Delia Goepfert, Hannah Weber, Benjamin Lambour, Marvin Sylvester, Christian Froeschel, Martin J. Mueller, Mohamed Osman, Frank Waller
Summary: Sphingolipid long chain bases (LCBs) in plants have antimicrobial activity and can inhibit growth of plant-interacting microorganisms. This study examined the inhibitory effect of the plant LCB phytosphingosine on various plant pathogens and symbiotic fungi, as well as bacterial pathogens. Phytosphingosine was found to effectively inhibit the growth of these organisms.
SCIENTIFIC REPORTS
(2022)
Review
Plant Sciences
Anup Kumar Sarkar, Sanjoy Sadhukhan
Summary: Plants produce VOCs in response to biotic stress for defense against pathogens, and AMFs can modulate plant defense through changes in VOC patterns. AMF-induced changes in plant chemistry and VOC emissions enhance plant immunity against microbial pathogens.
PHYSIOLOGIA PLANTARUM
(2023)
Article
Biochemistry & Molecular Biology
Baiying Li, Yonglun Zeng, Liwen Jiang
Summary: In eukaryotes, the endomembrane system allows for compartmentalization of cellular processes, with plant endomembrane system including various organelles like the endoplasmic reticulum and Golgi apparatus. COPII vesicles play a crucial role in anterograde transport from the endoplasmic reticulum to the Golgi apparatus and also have novel functions in autophagy. Recent research highlights the distinct roles of plant COPII vesicles in modulating autophagic flux and stress responses.
Article
Plant Sciences
Christian Loefke, David Scheuring, Kai Duenser, Maria Schoeller, Christian Luschnig, Juergen Kleine-Vehn
JOURNAL OF EXPERIMENTAL BOTANY
(2015)
Article
Multidisciplinary Sciences
David Scheuring, Christian Loefke, Falco Krueger, Maike Kittelmann, Ahmed Eisa, Louise Hughes, Richard S. Smith, Chris Hawes, Karin Schumacher, Juergen Kleine-Vehn
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Plant Sciences
Nathalie Mueller, Michaela Leroch, Julia Schumacher, David Zimmer, Anne Koennel, Klaus Klug, Thomas Leisen, David Scheuring, Frederik Sommer, Timo Muehlhaus, Michael Schroda, Matthias Hahn
Article
Biochemistry & Molecular Biology
Sabrina Kaiser, Ahmed Eisa, Juergen Kleine-Vehn, David Scheuring
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Editorial Material
Multidisciplinary Sciences
David Scheuring, Juergen Kleine-Vehn
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Review
Plant Sciences
Sabrina Kaiser, David Scheuring
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Plant Sciences
Marzieh Valifard, Rozenn Le Hir, Jonas Mueller, David Scheuring, Horst Ekkehard Neuhaus, Benjamin Pommerrenig
Summary: The SWEET17 gene plays a key role in regulating fructose signaling to modulate root growth during drought stress in Arabidopsis. This gene is predominantly expressed in the root vasculature and meristematic cells, and its activity integrates drought-induced cellular responses for maximal drought tolerance.
Article
Plant Sciences
Liuqing Kuang, Shangwu Chen, Yan Guo, David Scheuring, Moshe A. Flaishman, Huiqin Ma
Summary: In this study, the vacuole proteome of fig fruit flesh cells was analyzed at different developmental stages. The results showed significant changes in the vacuole proteome during fruit development, including decreased abundance of metabolic and transport proteins and increased abundance of proteins related to glycosidase, lipid, and extracellular proteins. This study expands our understanding of the vacuole proteome and its role in fruit development and quality formation.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Microbiology
Thomas Leisen, Janina Werner, Patrick Pattar, Nassim Safari, Edita Ymeri, Frederik Sommer, Michael Schroda, Ivonne Suarez, Isidro G. Collado, David Scheuring, Matthias Hahn
Summary: In this study, the researchers used the CRISPR/Cas technology to eliminate most of the known phytotoxic compounds in Botrytis cinerea strains. They found that these phytotoxic proteins play important roles in infection and host cell killing. The study provides insights into the functional complexity of fungal virulence factors and contributes to a comprehensive understanding of the infection mechanism of B. cinerea.
Article
Plant Sciences
Tanja Jeblick, Thomas Leisen, Christina E. Steidele, Isabell Albert, Jonas Mueller, Sabrina Kaiser, Florian Mahler, Frederik Sommer, Sandro Keller, Ralph Hueckelhoven, Matthias Hahn, David Scheuring
Summary: The secreted Botrytis protein hypersensitive response inducing protein 1 activates plant defense to kill plant cells. According to their lifestyle, plant pathogens are divided into biotrophic and necrotrophic organisms. However, the precise role of these proteins during infection is unknown.
Article
Plant Sciences
Elena Kreis, Justus Niemeyer, Marco Merz, David Scheuring, Michael Schroda
Summary: In plant cells, the CLP/HSP100 chaperone family member HSP101 plays a crucial role in resolving heat-induced protein aggregates. However, the role of the chloroplast family member CLPB3 in this process remains unclear. In this study, two clpb3 mutants in Chlamydomonas reinhardtii were characterized, revealing that chloroplast CLPB3 is required for resolving heat-induced protein aggregates and conferring thermotolerance. CLPB3 was found to interact with HSP22E/F near the thylakoid membranes, suggesting a possible role in disentangling protein aggregates from the thylakoid membrane system.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Biochemistry & Molecular Biology
Raphael Troesch, Fabian Ries, Lisa Desiree Westrich, Yang Gao, Claudia Herkt, Julia Hoppstaedter, Johannes Heck-Roth, Matthieu Mustas, David Scheuring, Yves Choquet, Markus Raeschle, Reimo Zoschke, Felix Willmund
Summary: Exposure to high temperatures causes temporary changes in chloroplast translation, specifically affecting antenna proteins of photosynthesis. This study reveals the sensitive regulatory principles involved in heat acclimation for the biogenesis and repair of chloroplast-encoded core subunits of photosynthetic complexes. The findings suggest that protein biosynthesis and translation elongation play central roles in heat acclimation and are conserved between different plant species.
Article
Biochemistry & Molecular Biology
Justus Niemeyer, David Scheuring, Julian Oestreicher, Bruce Morgan, Michael Schroda
Summary: In this study, a genetically encoded fluorescent H2O2 sensor was developed for monitoring H2O2 levels in various subcompartments of a Chlamydomonas cell. The results indicate the establishment of steep intracellular H2O2 gradients under normal physiological conditions with limited diffusion into other compartments.
Article
Biochemistry & Molecular Biology
Sabrina Kaiser, Sophie Eisele, David Scheuring
Summary: In Arabidopsis roots, the vacuole sizes and morphology are regulated by the hormone auxin through NET4A, affecting the cellular space utilization. Mutants and overexpressor lines of NET4A and NET4B still show sensitivity to auxin-induced vacuolar constrictions, suggesting the presence of additional regulatory mechanisms.
PLANT SIGNALING & BEHAVIOR
(2021)