Article
Oncology
Karen Kai-Lin Fang, Jongbok Lee, Ismat Khatri, Yoosu Na, Li Zhang
Summary: The use of allogeneic CAR4-DNTs as adoptive cell therapy for T-cell malignancies is effective. CAR4-DNTs can effectively target T-ALL and PTCL and have superior cytotoxicity compared to empty-vector transduced DNTs.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Oncology
Jeong A. Park, Madelyn Espinosa-Cotton, Hong-fen Guo, Sebastien Monette, Nai-Kong Cheung
Summary: This study demonstrates that using anti-VEGF antibodies can significantly improve the therapeutic efficacy of T cell immunotherapies by increasing HEVs and promoting the infiltration of cytotoxic CD8(+) TILs.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Biochemistry & Molecular Biology
Vita Golubovskaya, Hua Zhou, Feng Li, Robert Berahovich, Jinying Sun, Michael Valentine, Shirley Xu, Hizkia Harto, John Sienkiewicz, Yanwei Huang, Lijun Wu
Summary: This study focused on developing novel CS1 CAR-T cells and bispecific CS1-BCMA CAR-T cells for targeting multiple myeloma. The experimental results demonstrated that these cells effectively killed multiple myeloma cells, showing promise for future clinical trials.
Article
Oncology
Hongxia Wang, Liyan Wang, Yanning Li, Guangqi Li, Xiaochun Zhang, Dan Jiang, Yanting Zhang, Liyuan Liu, Yuankui Chu, Guangxian Xu
Summary: The study investigated the cytotoxicity of Nb-based CAR-T cells against lymphoma cells, demonstrating successful recognition and killing of target cells by these CAR-T cells. Similar results were observed with patient samples, indicating the potential of Nb CAR-T cells as a promising therapeutic strategy in future immunotherapy.
CANCER CELL INTERNATIONAL
(2021)
Article
Oncology
Camilla Bove, Silvia Arcangeli, Laura Falcone, Barbara Camisa, Rita El Khoury, Beatrice Greco, Anna De Lucia, Alice Bergamini, Attilio Bondanza, Fabio Ciceri, Chiara Bonini, Monica Casucci
Summary: This study investigated the role of CD4 and CD8 T cells in CD19 CAR-T cell responses and the development of cytokine release syndrome (CRS). CD4 CAR-T cells exhibited superior proliferation and activation potential compared to CD8 CAR-T cells. CD4 CAR-T cells were found to play a key role in the development of CRS, and CD4 CAR-T cells with embedded 4-1BB were associated with a safer profile. CD4 cells were crucial for maintaining long-term responses and the combination of CD4.BBz with CD8.28z CAR-T cells resulted in the lowest toxicity without compromising antitumor efficacy.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2023)
Article
Biochemistry & Molecular Biology
Tatsuya Hasegawa, Tomonori Oka, Heehwa G. Son, Valeria S. Oliver-Garcia, Marjan Azin, Thomas M. Eisenhaure, David J. Lieb, Nir Hacohen, Shadmehr Demehri
Summary: Senescent fibroblast accumulation in aging human skin is controlled by a virus-immune axis. Senescent fibroblasts increase in old skin, but not in the elderly, which is associated with increased recruitment of CXCL9 and cytotoxic CD4+ T cells. Skin-resident CD4 CTLs eliminate senescent fibroblasts expressing HLA-II and HCMV-gB through an HLA-II-dependent mechanism.
Article
Immunology
Aileen G. G. Rowan, Kanagaraju Ponnusamy, Hongwei Ren, Graham P. P. Taylor, Lucy B. M. Cook, Anastasios Karadimitris
Summary: This study aimed to validate the effectiveness of chimeric antigen receptor (CAR)-mediated cell therapy targeting specific T cell receptor beta chains in T cell lymphoma (TCL). The experimental results using CAR-T and CAR-iNKT cells demonstrated that this therapy method can selectively kill cancer cells while preserving most of the normal immune function.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Rene Platzer, Joschka Hellmeier, Janett Gohring, Iago Doel Perez, Philipp Schatzlmaier, Clara Bodner, Margarete Focke-Tejkl, Gerhard J. Schuetz, Eva Sevcsik, Hannes Stockinger, Mario Brameshuber, Johannes B. Huppa
Summary: This study demonstrates that single freely diffusing agonist pMHC II can autonomously elicit a full T-cell response in a peptide-specific manner, while endogenous pMHC II has minimal impact on TCR engagement.
Article
Biotechnology & Applied Microbiology
Sangwook Oh, Xuming Mao, Silvio Manfredo-Vieira, Jinmin Lee, Darshil Patel, Eun Jung Choi, Andrea Alvarado, Ebony Cottman-Thomas, Damian Maseda, Patricia Y. Y. Tsao, Christoph T. T. Ellebrecht, Sami L. L. Khella, David P. P. Richman, Kevin C. C. O'Connor, Uri Herzberg, Gwendolyn K. K. Binder, Michael C. C. Milone, Samik Basu, Aimee S. S. Payne
Summary: Engineered T cells expressing MuSK-CAART effectively target B cells expressing anti-MuSK autoantibodies, reducing muscle weakness caused by autoimmune disease.
NATURE BIOTECHNOLOGY
(2023)
Article
Immunology
Jia Feng, Haichan Xu, Andrew Cinquina, Zehua Wu, Wenli Zhang, Lihua Sun, Qi Chen, Lei Tian, Le Song, Kevin G. Pinz, Masayuki Wada, Xun Jiang, William M. Hanes, Yupo Ma, Hongyu Zhang
Summary: Researchers have modified CD4 CAR cells to secrete an IL-15/IL-15sushi complex, which has shown improved efficacy in targeting T-cell malignancies in preclinical and in vivo models. In a Phase I clinical trial, infusion of these modified cells was well-tolerated by patients with T-cell lymphomas and led to remission of the disease.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biology
Jinfeng Cai, Hongbo Gao, Jiacong Zhao, Shujing Hu, Xinyu Liang, Yanyan Yang, Zhuanglin Dai, Zhongsi Hong, Kai Deng
Summary: The research successfully established a highly physiologically relevant HIV-1 latency model by using a newly designed dual fluorescent reporter virus DFV-B, which can directly label latently infected cells and identified ACY-1215 as a potent latency reversing agent through high-throughput screening. This model provides a valuable tool to study critical events of HIV-1 latency and explore potential functional cures for AIDS.
Review
Immunology
Jarrod York, Kavitha Gowrishankar, Kenneth Micklethwaite, Sarah Palmer, Anthony L. Cunningham, Najla Nasr
Summary: Despite the effectiveness of ART in reducing morbidity and mortality associated with HIV infection, the latent HIV reservoir remains a major barrier to immune clearance and HIV cure. CAR T cell therapies, involving genetically engineered T cells, offer promising potential in targeting HIV-infected cells and have shown efficacy, safety, and long-term persistence in peripheral blood.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Ken Miyaguchi, Hongqiang Wang, Keith L. Black, Stephen L. Shiao, Rongfu Wang, John S. Yu
Summary: Efficient activation of T cells with tumor-associated antigens (TAAs) to glioblastoma (GBM) stem cells was achieved through the use of three different antigen sources loaded onto dendritic cells (DCs). An activated T cell (ATC) protocol was developed and optimized for a phase I clinical trial to target GSCs. The synthetic peptide pool loading method showed significantly increased cytotoxicity towards target cells.
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Theresa E. Pankhurst, Kaitlin H. Buick, Joshua L. Lange, Andrew J. Marshall, Kaileen R. Button, Olga R. Palmer, Kathryn J. Farrand, Isabelle Montgomerie, Thomas W. Bird, Ngarangi C. Mason, Joanna Kuang, Benjamin J. Compton, Davide Comoletti, Mariolina Salio, Vincenzo Cerundolo, Miguel E. Quinones-Mateu, Gavin F. Painter, Ian F. Hermans, Lisa M. Connor
Summary: Protective immune responses against respiratory pathogens largely depend on mucosal immunity, but most licensed vaccines fail to induce sufficient mucosal immunity. A suitable mucosal adjuvant has been lacking for the development of safe and effective mucosal vaccines. This study explores the use of mucosal-associated invariant T (MAIT) cells as adjuvants, showing that intranasal immunization with MAIT cell agonists and viral proteins can induce protective humoral immunity and IgA production.
Article
Oncology
Veronica L. Nagle, Charli Ann J. Hertz, Kelly E. Henry, Maya S. Graham, Carl Campos, Nagavarakishore Pillarsetty, Andrea Schietinger, Ingo K. Mellinghoff, Jason S. Lewis
Summary: This study explores the use of anti-human-CD4 minibody for antibody-based PET to visualize human CD4(+) T cells. Through in vitro and in vivo experiments, it is found that this method can accurately detect CD4(+) T cells without impacting their abundance, proliferation, and activation state. In humanized mice, this method can also visualize the distribution of CD4(+) T cells in peripheral tissues and brain tumors.
MOLECULAR CANCER THERAPEUTICS
(2022)
Article
Behavioral Sciences
Petra Bolte, Angelika Einwich, Pranav K. Seth, Raisa Chetverikova, Dominik Heyers, Irina Wojahn, Ulrike Janssen-Bienhold, Regina Feederle, Peter Hore, Karin Dedek, Henrik Mouritsen
Summary: The magnetic compass of birds is based on light-dependent radical-pair processes in the eyes, with cryptochromes being the primary candidate proteins for magnetoreceptor molecules. A study on various bird species confirmed the localization of Cry1a in the UV/V cone outer segments, suggesting accumulation of Cry1a molecules in distinct spots. No significant difference in Cry1a immunoreactivity was observed between light- and dark-adapted retinae, indicating that a C-terminal antibody against Cry1a does not specifically label a light-activated form of the protein.
ETHOLOGY ECOLOGY & EVOLUTION
(2021)
Correction
Multidisciplinary Sciences
Ansarullah, Chirag Jain, Fataneh Fathi Far, Sarah Homberg, Katharina Wissmiller, Felizitas Grafin von Hahn, Aurelia Raducanu, Silvia Schirge, Michael Sterr, Sara Bilekova, Johanna Siehler, Julius Wiener, Lena Oppenlaender, Amir Morshedi, Aimee Bastidas-Ponce, Gustav Collden, Martin Irmler, Johannes Beckers, Annette Feuchtinger, Michal Grzybek, Christin Ahlbrecht, Regina Feederle, Oliver Plettenburg, Timo D. Mueller, Matthias Meier, Matthias H. Tschoep, Unal Coskun, Heiko Lickert
Article
Multidisciplinary Sciences
Ansarullah, Chirag Jain, Fataneh Fathi Far, Sarah Homberg, Katharina Wissmiller, Felizitas Grafin von Hahn, Aurelia Raducanu, Silvia Schirge, Michael Sterr, Sara Bilekova, Johanna Siehler, Julius Wiener, Lena Oppenlaender, Amir Morshedi, Aimee Bastidas-Ponce, Gustav Collden, Martin Irmler, Johannes Beckers, Annette Feuchtinger, Michal Grzybek, Christin Ahlbrecht, Regina Feederle, Oliver Plettenburg, Timo D. Mueller, Matthias Meier, Matthias H. Tschoep, Uenal Coskun, Heiko Lickert
Summary: The discovery of inceptor, an inhibitor of insulin receptor (INSR) and IGF1 receptor (IGF1R) signalling in mouse beta-cells, sheds light on a potential molecular target for INSR-IGF1R sensitization and diabetes therapy. The study shows that inceptor plays a role in shielding insulin-producing beta-cells from constitutive pathway activation, which could have implications for improving glucose tolerance in vivo.
Article
Biochemistry & Molecular Biology
Antje Repenning, Daniela Happel, Caroline Bouchard, Marion Meixner, Yesim Verel-Yilmaz, Hartmann Raifer, Lena Holembowski, Eberhard Krause, Elisabeth Kremmer, Regina Feederle, Corinna U. Keber, Michael Lohoff, Emily P. Slater, Detlef K. Bartsch, Uta-Maria Bauer
Summary: The study reveals that PRMT1-mediated arginine methylation is crucial for triggering stress-induced tumor-suppressive function of p14(ARF). The interaction between PRMT1 and p14(ARF) leads to the release of p14(ARF) from nucleoli, promoting p53-independent apoptosis in response to genotoxic stress. This cooperation between PRMT1 and p14(ARF) has implications for cancer prognosis and chemotherapy response in pancreatic ductal adenocarcinoma.
Review
Allergy
Rauf Bhat, Fahad N. Almajhdi
Summary: Respiratory tract infections are the primary cause of morbidity and mortality globally, with a lack of understanding on the mechanisms of latency, persistence, and reinfection of Bocavirus. The virus mainly targets type I IFN pathways to evade the immune system, and vaccination strategies such as antisense technology can combat Bocavirus infections.
INTERNATIONAL ARCHIVES OF ALLERGY AND IMMUNOLOGY
(2021)
Article
Virology
Mohamed A. Farrag, Haitham M. Amer, Rauf Bhat, Fahad N. Almajhdi
Summary: MERS-CoV is continuously evolving, with the highest mutation rates observed in the S gene. Selective pressure was found on certain codons, and characteristic amino acid changes were identified across the S protein, showing potential host and year specificity. Phylogenetic analysis based on the S gene sequence revealed clustering of MERS-CoV strains into three main clades, with subdivision of clade B into B1 to B4. Regular analysis of the molecular and pathobiological characteristics of future MERS-CoV strains is recommended to prevent potential future outbreaks at early phases.
Article
Multidisciplinary Sciences
Kai P. Hoefig, Alexander Reim, Christian Gallus, Elaine H. Wong, Gesine Behrens, Christine Conrad, Meng Xu, Lisa Kifinger, Taku Ito-Kureha, Kyra A. Y. Defourny, Arie Geerlof, Josef Mautner, Stefanie M. Hauck, Dirk Baumjohann, Regina Feederle, Matthias Mann, Michael Wierer, Elke Glasmacher, Vigo Heissmeyer
Summary: Post-transcriptional gene regulation in T cells is a dynamic and complex process, involving multiple RNA-binding proteins and unexpected functions for certain transcription factors and signal transducers. By studying the regulation of Roquin-1 and Roquin-2, it was found that multiple RBPs contribute to the coregulation of mRNA targets by individual trans-acting factors.
NATURE COMMUNICATIONS
(2021)
Review
Environmental Sciences
Mohamed A. Farrag, Haitham M. Amer, Rauf Bhat, Maaweya E. Hamed, Ibrahim M. Aziz, Ayman Mubarak, Turki M. Dawoud, Sami G. Almalki, Fayez Alghofaili, Ahmad K. Alnemare, Raid Saleem Al-Baradi, Bandar Alosaimi, Wael Alturaiki
Summary: The SARS-CoV-2 outbreak, originating in Wuhan, China in December 2019, is the third and possibly the worst pandemic caused by human coronaviruses. The virus, officially named SARS-CoV-2, has a highly mutable genome allowing it to rapidly adapt and evolve, and its animal reservoir and intermediate host remain unresolved. Future spillover of coronaviruses from bats is a concern, highlighting the importance of establishing intervention approaches to prevent such occurrences.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Article
Biochemistry & Molecular Biology
Shan Xin, Constanze Mueller, Susanne Pfeiffer, Vanessa A. N. Kraft, Juliane Merl-Pham, Xuanwen Bao, Regina Feederle, Xiang Jin, Stefanie M. Hauck, Philippe Schmitt-Kopplin, Joel A. Schick
Summary: This study uncovers a crucial link between ferroptosis and calcium through the identification of the novel tetraspanin MS4A15; MS4A15 blocks ferroptosis by depleting luminal calcium stores and reprogramming membrane phospholipids; Increasing luminal calcium levels can sensitize refractory cancer cell lines.
CELL DEATH AND DIFFERENTIATION
(2022)
Article
Behavioral Sciences
Angelika Einwich, Pranav Kumar Seth, Rabea Bartoelke, Petra Bolte, Regina Feederle, Karin Dedek, Henrik Mouritsen
Summary: Research has shown that Cry2 protein is located in different layers of cells in the retina of night-migratory European robins, homing pigeons, and domestic chickens, and at a subcellular level, it is present in both the cytoplasm and the nucleus of these cells.
JOURNAL OF COMPARATIVE PHYSIOLOGY A-NEUROETHOLOGY SENSORY NEURAL AND BEHAVIORAL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Eva Scholler, James Marks, Virginie Marchand, Astrid Bruckmann, Christopher A. Powell, Markus Reichold, Christian Daniel Mutti, Katja Dettmer, Regina Feederle, Stefan Huttelmaier, Mark Helm, Peter Oefner, Michal Minczuk, Yuri Motorin, Markus Hafner, Gunter Meister
Summary: The mitochondrial protein METTL8 plays a crucial role in facilitating m(3)C methylation on mt-tRNAs, impacting respiratory chain activity and pancreatic cancer development. This modification leads to optimal composition and function of the mitochondria respiratory chain, with implications for patient survival and cellular metabolism.
Review
Medicine, General & Internal
Rauf Bhat, Sameh S. M. Soliman, Magdy M. El-Sayed Ahmed, Mohamed Husseiny
Summary: The outbreak of the current global pandemic caused by the novel coronavirus SARS-CoV-2 has posed an unprecedented threat to global health and economy. Scientists and clinicians are working tirelessly to develop effective treatments and vaccines to combat this deadly pathogen. In addition to vaccine research, existing drugs are being repurposed to test their efficacy against SARS-CoV-2.
RUSSIAN OPEN MEDICAL JOURNAL
(2021)
Article
Cell Biology
Stefan Preisendoerfer, Yoshihiro Ishikawa, Elisabeth Hennen, Stephan Winklmeier, Jonas C. Schupp, Larissa Knueppel, Isis E. Fernandez, Leonhard Binzenhofer, Andrew Flatley, Brenda M. Juan-Guardela, Clemens Ruppert, Andreas Guenther, Marion Frankenberger, Rudolf A. Hatz, Nikolaus Kneidinger, Jurgen Behr, Regina Feederle, Aloys Schepers, Anne Hilgendorff, Naftali Kaminski, Edgar Meinl, Hans Peter Baechinger, Oliver Eickelberg, Claudia A. Staab-Weijnitz
Summary: The study reveals that FKBP11 plays an important role in plasma cells, is regulated by XBP1, and sheds light on the important mechanisms involved in antibody production and folding.
Article
Immunology
Georgia Papadogianni, Inga Ravens, Ahmed Hassan, Andrew Flatley, Regina Feederle, Guenter Bernhardt, Hristo Georgiev
Summary: Neuritin is a crucial neurotrophic factor involved in neuronal development and plasticity, as well as endothelial angiogenesis, cell migration, tumor growth, and antibody production. Monoclonal mouse anti-mouse neuritin antibodies were generated by immunizing knock-out mice with two different neuritin-derived peptides. These antibodies can recognize neuritin in various species, including humans, and are useful for studying neuritin expression and function, particularly in murine follicular regulatory T cells and germinal center B cells.
Article
Biochemistry & Molecular Biology
Katrin Gruner, Franz Leissing, Dzmitry Sinitski, Hannah Thieron, Christian Axstmann, Kira Baumgarten, Anja Reinstadler, Pascal Winkler, Melina Altmann, Andrew Flatley, Maelle Jaouannet, Krzysztof Zienkiewicz, Ivo Feussner, Harald Keller, Christine Coustau, Pascal Falter-Braun, Regina Feederle, Jurgen Bernhagen, Ralph Panstruga
Summary: The research provides an in planta characterization and functional analysis of the three-member gene/protein MDL family in Arabidopsis thaliana. The study shows that MDL1, MDL2, and MDL3 form homo-oligomers and hetero-oligomers, and play a role in regulating both developmental and immune-related processes in plants. The findings expand the comparative cross-kingdom analysis of MIF/MDL signaling in human and plant systems.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)