Article
Plant Sciences
Mil Thi Tran, Geon Hui Son, Young Jong Song, Ngan Thi Nguyen, Seonyeong Park, Thanh Vu Thach, Jihae Kim, Yeon Woo Sung, Swati Das, Dibyajyoti Pramanik, Jinsu Lee, Ki-Ho Son, Sang Hee Kim, Tien Van Vu, Jae-Yean Kim
Summary: CRISPR-Cas9-based genome editing was used to edit a tomato gene, resulting in the elimination of the PRD and 8CM domains. The edited tomato plants showed increased tolerance to various stresses, including high temperature, osmotic stress, and Pseudomonas syringae pv. tomato infection.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Plant Sciences
Adnan Rasheed, Huijie Li, Muhammad Nawaz, Athar Mahmood, Muhammad Umair Hassan, Adnan Noor Shah, Fiaz Hussain, Saira Azmat, Syed Faheem Anjum Gillani, Yasir Majeed, Sameer H. Qari, Ziming Wu
Summary: Improving salinity tolerance in rice can reduce yield losses caused by stress. Conventional breeding methods have limitations in developing salt-tolerant rice genotypes, making it critical to use molecular tools for screening salt-tolerant genes. The latest molecular techniques have shown reliability in incorporating salt tolerance in rice at the molecular level. Large-scale use of these genetic approaches helps identify and edit genes/QTLs associated with salinity tolerance in rice. Molecular breeding tools are also essential for conserving rice genetic diversity.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Meenakshi Rajput, Khushboo Choudhary, Manish Kumar, V. Vivekanand, Aakash Chawade, Rodomiro Ortiz, Nidhi Pareek
Summary: With the growth in population, the demand for food is increasing, leading to the development of innovative crop improvement approaches such as RNA interference (RNAi) and the CRISPR/Cas9 system. These technologies have shown promise in enhancing crop yields and increasing tolerance to environmental stress.
Review
Environmental Sciences
Misbah Naz, Adalberto Benavides-Mendoza, Muhammad Tariq, Jianyu Zhou, Jiahao Wang, Shanshan Qi, Zhicong Dai, Daolin Du
Summary: Phytoremediation is an active research field that focuses on identifying and characterizing novel super-accumulators with high chelation action. Molecular tools and CRISPR-Cas9 technology are widely employed to enhance metal absorption, translocation, and tolerance mechanisms in plants for detoxification. Future research in adaptive phytoremediation ability and sustainable ecosystem will be triggered by advancements in molecular science.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Agronomy
Naglaa A. Abdallah, Hany Elsharawy, Hamiss A. Abulela, Roger Thilmony, Abdelhadi A. Abdelhadi, Nagwa I. Elarabi
Summary: Genome editing using a multiplex sgRNA-CRISPR/Cas9 system has been utilized to generate wheat mutants with TaSal1 gene mutations, resulting in phenotypic changes such as rolled leaves and bent stems. Anatomical and microscopic analysis of the mutated plants showed alterations in stomata and bulliform cells. This study demonstrates the efficiency of multiplex sgRNA-CRISPR/Cas9 genome editing in inducing mutations in hexaploid wheat.
GM CROPS & FOOD-BIOTECHNOLOGY IN AGRICULTURE AND THE FOOD CHAIN
(2022)
Review
Biochemistry & Molecular Biology
Muhammad Khuram Razzaq, Muqadas Aleem, Shahid Mansoor, Mueen Alam Khan, Saeed Rauf, Shahid Iqbal, Kadambot H. M. Siddique
Summary: Through omics and CRISPR-Cas9 technology, plants can better respond to various stress conditions, thereby improving crop yield and quality.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Entomology
Vera M. Yilmaz, Timothy J. S. Ramnarine, Annabella Koeniger, Selina Mussgnug, Sonja Grath
Summary: Ectotherms, such as insects, have body temperatures that depend on ambient temperature, and temperature variations affect the distribution of these species. Insects provide a good study system for investigating adaptation to temperature changes, and the vinegar fly Drosophila ananassae is being used as a model to study the genetic basis of cold tolerance. Molecular genetic tools, such as CRISPR/Cas9 and the PiggyBac system, have been established to assess the function of candidate genes associated with cold tolerance. The integration of Cas9 enzyme did not consistently affect cold tolerance in this species.
JOURNAL OF INSECT PHYSIOLOGY
(2023)
Article
Plant Sciences
Yunqing Cheng, Yujie Li, Jing Yang, Hongli He, Xingzheng Zhang, Jianfeng Liu, Xiangdong Yang
Summary: Gene editing of EIL family members in soybeans, particularly for EIL3, EIL4, and EIN2L, resulted in earlier flowering and increased yield. Sucrose synthase, AUX28, MADS3, type-III polyketide synthase A/B, ABC transporter G family member 26, tetraketide alpha-pyrone reductase, and fatty acyl-CoA reductase 2 may be involved in regulating early flowering and high-yield phenotypes in triple mutant soybean plants.
Review
Biochemistry & Molecular Biology
Sangeeta Banerjee, Ananya Mukherjee, Atreyee Kundu
Summary: The primary goal of plant biotechnologists is to produce a designer crop with added attributes. Genetic engineering allows the displacement of genes among species, and the introduction of foreign genes can create new traits in the host genome. Oilseed mustard varieties have been successfully transformed with genes from a wide range of species, resulting in improved yield and value. However, the genetic transformation of oilseed mustard still faces challenges, and further research is needed to improve the transformation system.
MOLECULAR BIOLOGY REPORTS
(2023)
Review
Biochemical Research Methods
Hui Peng, Yi Zheng, Zhixun Zhao, Jinyan Li
Summary: CRISPR/Cas9 multigene editing is a widely studied topic in biomedicine and biology, requiring optimization of sgRNA selection to minimize off-target editing effects. While off-target editing is unavoidable, some sgRNAs may turn into on-target editing sites for other genes of interest, demonstrating the potential benefits of preference cutting score evaluation.
BRIEFINGS IN BIOINFORMATICS
(2021)
Article
Plant Sciences
Adnan Rasheed, Anwar Ahmad Khan, Muhammad Nawaz, Athar Mahmood, Uzma Arif, Muhammad Umair Hassan, Javed Iqbal, Muhammad Hamzah Saleem, Basharat Ali, Shah Fahad
Summary: Soybean, a significant oilseed and food crop, is vulnerable to abiotic stresses, with aluminum toxicity being the most serious threat. Advances in molecular biology have identified potential genes and QTL involved in soybean response to aluminum stress, but genetic erosion poses a risk to the beneficial alleles. Molecular techniques like transcription factors and genetic engineering have been used, but the understanding of aluminum tolerance mechanism is still incomplete. More efforts should be made to breed aluminum-tolerant cultivars by targeting the identified genes/QTL.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Agronomy
Zhaobo Li, Qun Cheng, Zhuoran Gan, Zhihong Hou, Yuhang Zhang, Yongli Li, Haiyang Li, Haiyang Nan, Cen Yang, Linnan Chen, Sijia Lu, Wenqian Shi, Liyu Chen, Yanping Wang, Chao Fang, Liping Kong, Tong Su, Shichen Li, Kun Kou, Lingshuang Wang, Fanjiang Kong, Baohui Liu, Lidong Dong
Summary: The study successfully shortened the flowering time in soybean by targeted mutagenesis of the LNK2 genes using CRISPR/Cas9 technology, potentially beneficial for soybean breeding in high-latitude environments.
Article
Plant Sciences
Zhiqun Que, Qineng Lu, Qixiu Li, Chunxiu Shen
Summary: This study provides a comprehensive account of the cloning and functional characterization of the rice annexin gene OsAnn5. The results reveal that OsAnn5 is involved in cold stress tolerance at the seedling stage by participating in the endoplasmic reticulum apparatus. These findings have important implications for understanding plant stress responses and related cellular processes.
Article
Plant Sciences
Li Cao, Zeru Wang, Hongyu Ma, Tengfei Liu, Jing Ji, Kaixuan Duan
Summary: This research demonstrates the efficient reduction of raffinose family oligosaccharides (RFOs) in soybean seeds by using the multiplex Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9-mediated gene editing system. The knockout of soybean raffinose synthase (RS) genes RS2 and RS3 simultaneously leads to significantly reduced levels of RFOs in mature seeds without any negative impact on plant growth and development.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Agronomy
Adnan Rasheed, Hongdong Jie, Pengliang He, Xueying Lv, Basharat Ali, Yushen Ma, Hucheng Xing, Saad Almari, Rehab O. Elnour, Muhammad Umair Hassan, Syed Faheem Anjum Gillani, Yucheng Jie
Summary: This review discusses methods to improve Cd tolerance in ramie and points out the limitations of current research. It suggests transferring or editing identified Cd-tolerant genes/QTL into sensitive cultivars using genetic engineering or CRISPR/Cas9. The use of CRISPR/Cas9 is highly recommended for targeted gene editing, as it can address research gaps and revolutionize the field of agriculture.
Article
Biochemical Research Methods
Indrani Baruah, Geetanjali Baruah, Jagajjit Sahu, Dhanawantari L. Singha, Hariprasanna Dekaboruah, Natarajan Velmurugan, Channakeshavaiah Chikkaputtaiah
PLANT MOLECULAR BIOLOGY REPORTER
(2020)
Article
Plant Sciences
Banashree Saikia, Johni Debbarma, Jitendra Maharana, Dhanawantari L. Singha, Natarajan Velmuruagan, Hariprasanna Dekaboruah, Kallare P. Arunkumar, Channakeshavaiah Chikkaputtaiah
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2020)
Review
Green & Sustainable Science & Technology
Aparna Choudhury, Jiumoni Lahkar, Binoy Kumar Saikia, Anil Kumar Anil Singh, Channakeshavaiah Chikkaputtaiah, Hari Prasanna Deka Boruah
Summary: The mining of coal has significant environmental impacts, which can be mitigated through successful methodologies used globally. However, specific adjustments may be necessary for different locations. In the northeastern coalfields of Assam, attention should be paid to reclamation measures and strategies to effectively address environmental issues caused by mining activities.
ENVIRONMENT DEVELOPMENT AND SUSTAINABILITY
(2021)
Article
Plant Sciences
Johni Debbarma, Banashree Saikia, Dhanawantari L. Singha, Jitendra Maharana, Natarajan Velmuruagan, Hariprasanna Dekaboruah, Kallare P. Arunkumar, Channakeshavaiah Chikkaputtaiah
Summary: The study identified Xylem sap protein 10 (XSP10) and Salicylic acid methyl transferase (SlSAMT) as potential negative regulatory genes associated with Fusarium wilt of tomato. The expression analysis showed higher expression of XSP10 and SlSAMT in root and flower tissue of different tomato cultivars, with stronger up-regulation in a susceptible cultivar compared to a multiple disease resistant cultivar, indicating the disease susceptibility nature of these genes. Sub-cellular localization analysis revealed that XSP10 mainly localized in the nucleus and SlSAMT in the cytoplasm, and a strong in vivo protein-protein interaction between XSP10 and SlSAMT in regulating responses to Fusarium wilt tolerance in tomato.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2021)
Article
Plant Sciences
Natarajan Velmurugan, Yesupatham Sathishkumar, Shashanka Sonowal, Ka-Lai Pang, Yang Soo Lee
Summary: Two new thraustochytrid isolates from Taiwan mangroves, Aurantiochytrium sp. IMB169 and Aurantiochytrium sp. IMB171, were characterized for their long-chain saturated and polyunsaturated fatty acids using transmission electron microscopy and flow cytometry with BODIPY staining. The study revealed a progressive accumulation of lipid products in both isolates, with IMB169 showing a high level of docosahexaenoic acid. Qualitative and analytical results were validated by gas chromatography, and a quantitative baseline was established for efficient bioprocessing methodology development.
Article
Genetics & Heredity
Riwandahun Marwein, Sanjay Singh, Jitendra Maharana, Sanjeev Kumar, Kallare P. Arunkumar, Natarajan Velmurugan, Channakeshavaiah Chikkaputtaiah
Summary: The study identified different levels of resistance in North-East Indian rice cultivars to Bipolaris oryzae, and revealed the importance of early response to the pathogen in suppressing disease progression through systematic RNA seq analysis. The findings provide key regulatory genes for potential functional genetic analysis to develop genetic tolerance to BSD of rice.
Article
Soil Science
Shashanka Sonowal, Refad Ahmed, Channakeshavaiah Chikkaputtaiah, Egam Basar, Natarajan Velmurugan
Summary: This study aimed to comprehensively analyze the under-explored endophytic bacterial community of P. polyphylla and identified different characteristics of the isolates. The draft genome of the potential PGP endophyte Bacillus paralicheniformis PPE102 was obtained and showed positive impacts on the growth of Micractinium sp. GA001.
APPLIED SOIL ECOLOGY
(2022)
Article
Genetics & Heredity
Johni Debbarma, Banashree Saikia, Dhanawantari L. L. Singha, Debajit Das, Ajay Kumar Keot, Jitendra Maharana, Natarajan Velmurugan, Kallare P. P. Arunkumar, Palakolanu Sudhakar Reddy, Channakeshavaiah Chikkaputtaiah
Summary: Fusarium wilt is a devastating fungal disease in tomato caused by Fusarium oxysporum f. sp. lycopersici, leading to reduced yield and production. Xylem sap protein 10 (XSP10) and Salicylic acid methyl transferase (SlSAMT) are two negative regulatory genes associated with Fusarium wilt in tomato. CRISPR/Cas9 has emerged as a promising technique to target these susceptible genes and increase tolerance/resistance to plant diseases. In this study, the efficiency of CRISPR/Cas9-mediated editing of XSP10 and SlSAMT genes was validated in tomato, and dual-gene editing showed stronger tolerance to Fusarium wilt than single-gene editing. Stable transformation of tomato further confirmed that dual-gene CRISPR-edited lines exhibited higher tolerance to Fusarium wilt compared to single-gene-edited lines.
Review
Plant Sciences
Diganta Kumar Saikia, Channakeshavaiah Chikkaputtaiah, Natarajan Velmurugan
Summary: Long-chain polyunsaturated fatty acids (LC-PUFA) are widely recognized beneficial compounds used as nutrient supplements, with a market value expected to reach $9.7 billion by 2032. However, the global PUFA supply chain is severely hampered by the decline in natural fish catch and global climate change. To meet the global demand, low-cost production strategies and efficient cell lines specific for marine zoosporic microbes are crucial. This review summarizes production and strain improvement strategies, and highlights the commercial prospects for marine zoosporic microbe-derived PUFA.
Article
Mycology
Sanjay Singh, Yogita N. Sarki, Riwandahun Marwein, Dhanawantari L. Singha, Natarajan Velmurugan, Channakeshavaiah Chikkaputtaiah
Summary: This study used time-course transcriptome sequencing to investigate the differential transcriptional responses of Bipolaris oryzae in two rice hosts with contradictory infection responses. The results revealed similar regulation of fungal genes in susceptible and resistant hosts at an early stage, but a significantly higher number of upregulated genes were found in susceptible hosts at a later stage. Enrichment analysis showed that terms related to carbohydrate degradation, carbohydrate transport, and pathogenesis were extensively enriched in susceptible hosts at a later stage. The expression responses of secretory and effector proteins were also similar between the two hosts.
Article
Plant Sciences
Sudarshana Borah, Banashree Saikia, Dipsikha Bora, Priyadarshini Bhorali
Summary: This study analyzed the expression of defense-related genes in response to infection by the Sigatoka pathogen in a resistant banana cultivar. The findings showed upregulation of important defense response genes after infection, providing valuable insights for genetic control of the disease.
INDIAN JOURNAL OF GENETICS AND PLANT BREEDING
(2022)