When Small becomes Too Big: Expanding the Use of In‐Cell Solid‐State NMR Spectroscopy

Direct observation of dynamic protein interactions involving human microtubules using solid-state NMR spectroscopy

(2020) Yanzhang Luo et al.   Nature Communications

In-Cell EPR Distance Measurements on Ubiquitin Labeled with a Rigid PyMTA-Gd(III) Tag

(2019) Yin Yang et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Quo Vadis Biomolecular NMR Spectroscopy?

(2019) Philipp Selenko   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

High-Resolution Protein 3D Structure Determination in Living Eukaryotic Cells

(2019) Takashi Tanaka et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

DNP-Supported Solid-State NMR Spectroscopy of Proteins Inside Mammalian Cells

(2019) Siddarth Narasimhan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Toward high-resolution in situ structural biology with cryo-electron tomography and subtomogram averaging

(2019) Florian KM Schur   CURRENT OPINION IN STRUCTURAL BIOLOGY

Real-Time NMR Spectroscopy for Studying Metabolism

(2019) Islam Alshamleh et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Regulation of α-synuclein by chaperones in mammalian cells

(2019) Björn M. Burmann et al.   NATURE

In-Cell NMR in Human Cells: Direct Protein Expression Allows Structural Studies of Protein Folding and Maturation

(2018) Enrico Luchinat et al.   ACCOUNTS OF CHEMICAL RESEARCH

Dynamic Nuclear Polarization Nuclear Magnetic Resonance in Human Cells Using Fluorescent Polarizing Agents

(2018) Brice J. Albert et al.   BIOCHEMISTRY

Combined 1 H-Detected Solid-State NMR Spectroscopy and Electron Cryotomography to Study Membrane Proteins across Resolutions in Native Environments

(2018) Lindsay A. Baker et al.   STRUCTURE

Cryo-electron microscopy shapes up

(2018) Monya Baker   NATURE

High-resolution NMR studies of antibiotics in cellular membranes

(2018) João Medeiros-Silva et al.   Nature Communications

Dynamic Nuclear Polarization Signal Enhancement with High-Affinity Biradical Tags

(2017) Rivkah Rogawski et al.   JOURNAL OF PHYSICAL CHEMISTRY B

Studying assembly of the BAM complex in native membranes by cellular solid-state NMR spectroscopy

(2017) Cecilia Pinto et al.   JOURNAL OF STRUCTURAL BIOLOGY

Efficient assignment and NMR analysis of an intact virus using sequential side-chain correlations and DNP sensitization

(2017) Ivan V. Sergeyev et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The structure of the COPI coat determined within the cell

(2017) Yury S Bykov et al.   eLife

Selective Protein Hyperpolarization in Cell Lysates Using Targeted Dynamic Nuclear Polarization

(2016) Thibault Viennet et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

1 H-Detected Solid-State NMR Studies of Water-Inaccessible Proteins In Vitro and In Situ

(2016) João Medeiros-Silva et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

EGFR Dynamics Change during Activation in Native Membranes as Revealed by NMR

(2016) Mohammed Kaplan et al.   CELL

Quantitative FRET studies and integrative modeling unravel the structure and dynamics of biomolecular systems

(2016) Mykola Dimura et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

High resolution observed in 800 MHz DNP spectra of extremely rigid type III secretion needles

(2016) Pascal Fricke et al.   JOURNAL OF BIOMOLECULAR NMR

Structural disorder of monomeric α-synuclein persists in mammalian cells

(2016) Francois-Xavier Theillet et al.   NATURE

CATH: an expanded resource to predict protein function through structure and sequence

(2016) Natalie L. Dawson et al.   NUCLEIC ACIDS RESEARCH

Nuclear magnetic resonance (NMR) applied to membrane–protein complexes

(2016) Mohammed Kaplan et al.   QUARTERLY REVIEWS OF BIOPHYSICS

Intracellular repair of oxidation-damaged α-synuclein fails to target C-terminal modification sites

(2016) Andres Binolfi et al.   Nature Communications

An Efficient Labelling Approach to Harness Backbone and Side-Chain Protons in1H-Detected Solid-State NMR Spectroscopy

(2015) Deni Mance et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Efficient Dynamic Nuclear Polarization at 800 MHz/527 GHz with Trityl-Nitroxide Biradicals

(2015) Guinevere Mathies et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Cellular solid-state NMR investigation of a membrane protein using dynamic nuclear polarization

(2015) Kazutoshi Yamamoto et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES

In Situ Structural Studies of Anabaena Sensory Rhodopsin in the E. coli Membrane

(2015) Meaghan E. Ward et al.   BIOPHYSICAL JOURNAL

Sensitivity-Enhanced NMR Reveals Alterations in Protein Structure by Cellular Milieus

(2015) Kendra K. Frederick et al.   CELL

Biomolecular DNP-Supported NMR Spectroscopy using Site-Directed Spin Labeling

(2015) Elwin A. W. van der Cruijsen et al.   CHEMISTRY-A EUROPEAN JOURNAL

Efficient cellular solid-state NMR of membrane proteins by targeted protein labeling

(2015) Lindsay A. Baker et al.   JOURNAL OF BIOMOLECULAR NMR

The magnetic field dependence of cross-effect dynamic nuclear polarization under magic angle spinning

(2015) Deni Mance et al.   JOURNAL OF CHEMICAL PHYSICS

Probing a cell-embedded megadalton protein complex by DNP-supported solid-state NMR

(2015) Mohammed Kaplan et al.   NATURE METHODS

Correlated light and electron microscopy: ultrastructure lights up!

(2015) Pascal de Boer et al.   NATURE METHODS

Sterically shielded spin labels for in-cell EPR spectroscopy: Analysis of stability in reducing environment

(2014) A. P. Jagtap et al.   FREE RADICAL RESEARCH

NMR-based structural biology enhanced by dynamic nuclear polarization at high magnetic field

(2014) Eline J. Koers et al.   JOURNAL OF BIOMOLECULAR NMR

Atomic Model of a Cell-Wall Cross-Linking Enzyme in Complex with an Intact Bacterial Peptidoglycan

(2014) Paul Schanda et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Proton Clouds to Measure Long-Range Contacts between Nonexchangeable Side Chain Protons in Solid-State NMR

(2014) Tessa Sinnige et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

High Frequency Dynamic Nuclear Polarization

(2013) Qing Zhe Ni et al.   ACCOUNTS OF CHEMICAL RESEARCH

Highly Efficient, Water-Soluble Polarizing Agents for Dynamic Nuclear Polarization at High Frequency

(2013) Claire Sauvée et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Site-Specific Interaction between α-Synuclein and Membranes Probed by NMR-Observed Methionine Oxidation Rates

(2013) Alexander S. Maltsev et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

M2 Proton Channel Structural Validation from Full-Length Protein Samples in Synthetic Bilayers and E. coli Membranes

(2012) Yimin Miao et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Solid-State NMR Spectroscopy on Cellular Preparations Enhanced by Dynamic Nuclear Polarization

(2012) Marie Renault et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

In-Cell Solid-State NMR as a Tool to Study Proteins in Large Complexes

(2012) Sina Reckel et al.   CHEMBIOCHEM

Cellular solid-state nuclear magnetic resonance spectroscopy

(2012) M. Renault et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Protein N-terminal acetyltransferases: when the start matters

(2012) Kristian K. Starheim et al.   TRENDS IN BIOCHEMICAL SCIENCES

Characterization of Membrane Proteins in Isolated Native Cellular Membranes by Dynamic Nuclear Polarization Solid-State NMR Spectroscopy without Purification and Reconstitution

(2011) Tomas Jacso et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Fractional deuteration applied to biomolecular solid-state NMR spectroscopy

(2011) Deepak Nand et al.   JOURNAL OF BIOMOLECULAR NMR

Monitoring Real-Time Metabolism of Living Cells by Fast Two-Dimensional NMR Spectroscopy

(2010) Andrea Motta et al.   ANALYTICAL CHEMISTRY

Rapid screening for structural integrity of expressed proteins by heteronuclear NMR spectroscopy

(2010) Angela M. Gronenborn et al.   PROTEIN SCIENCE

Observation of NMR Signals from Proteins Introduced into Living Mammalian Cells by Reversible Membrane Permeabilization Using a Pore-Forming Toxin, Streptolysin O

(2009) Shinji Ogino et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Protein structure determination in living cells by in-cell NMR spectroscopy

(2009) Daisuke Sakakibara et al.   NATURE

High-resolution multi-dimensional NMR spectroscopy of proteins in human cells

(2009) Kohsuke Inomata et al.   NATURE

In situ observation of protein phosphorylation by high-resolution NMR spectroscopy

(2008) Philipp Selenko et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY