Article
Multidisciplinary Sciences
Madeline M. Schwarz, David A. Price, Safder S. Ganaie, Annie Feng, Nawneet Mishra, Ryan M. Hoehl, Farheen Fatma, Sarah H. Stubbs, Sean P. J. Whelan, Xiaoxia Cui, Takeshi Egawa, Daisy W. Leung, Gaya K. Amarasinghe, Amy L. Hartman
Summary: This study reveals that Oropouche orthobunyavirus (OROV) utilizes the host protein low-density lipoprotein-related protein 1 (Lrp1) for efficient cellular infection. Additionally, the study demonstrates the biological relevance of the OROV-Lrp1 interaction in a mouse model, providing potential therapeutic strategies for bunyavirus infections.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Luke R. R. Green, Rahaf Issa, Fawzyah Albaldi, Lucy Urwin, Ruth Thompson, Henna Khalid, Claire E. E. Turner, Barbara Ciani, Lynda J. J. Partridge, Peter N. N. Monk
Summary: This article investigates the role of the host membrane adapter protein CD9 in Staphylococcus aureus adhesion to epithelial cells. The study demonstrates that CD9 is essential for syndecan-mediated bacterial adhesion and that it coordinates with fibronectin to promote bacterial adherence and internalization. Insights from this research may lead to the development of alternative anti-adhesion treatments for staphylococcal infections.
Article
Neurosciences
Luisa Speranza, Yanis Inglebert, Claudia De Sanctis, Pei You Wu, Magdalena Kalinowska, R. Anne McKinney, Anna Francesconi
Summary: This study found that mGluR-LTD occurs in spines containing Synaptopodin/SA, while spines lacking Synaptopodin/SA are selectively lost. This process is dependent on mGluR1 and can selectively preserve stronger spines while eliminating weaker ones through mechanisms like physical retention and protein degradation.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Cell Biology
Colette B. Rogers, Rachel E. Kram, Kevin Lin, Chad L. Myers, Alexandra Sobeck, Eric A. Hendrickson, Anja-Katrin Bielinsky
Summary: Activation of the Fanconi anemia (FA) pathway is crucial for preventing chromosome translocations referred to as "radials" after treatment with mitomycin C (MMC). Radials induced by MMC are dependent on DNA polymerase theta (POLq)-mediated alternative end joining (A-EJ) in FA-deficient cells, specifically involving POL theta and DNA ligase III. Blocking POLq in FA-deficient cells eliminates radials and leads to the accumulation of breaks at common fragile sites. These findings provide mechanistic insights into the formation of radials and offer potential therapeutic strategies for FA pathway-deficient cancers using POLq inhibitors.
Article
Biochemistry & Molecular Biology
Jasmine A. Burrell, Jacqueline M. Stephens
Summary: KAT8 is essential for adipocyte differentiation, with its loss inhibiting the formation of adipocytes in the early stages. However, its absence does not significantly impact later stages of adipocyte differentiation. Further studies are needed to determine the specific functions and regulation of KAT8 in adipose tissue.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Immunology
Annie Qiu, Anabel Miller, Flavia Dei Zotti, Manjula Santhanakrishnan, Jeanne E. Hendrickson, Maria Tredicine, Sean R. Stowell, Chance John Luckey, James C. Zimring, Krystalyn E. Hudson
Summary: Passive immunization with anti-D can prevent maternal alloimmunization to RhD, but it may fail in some cases and even enhance alloimmunization. This study identified that FcγRIV on splenic conventional dendritic cells (cDCs) is required for IgG2c-mediated enhancement of RBC alloantibody production. FcγRI can cause RBC clearance without increasing alloimmunization. These findings have implications for the standardization of antibody subclasses in immunoprophylaxis preparations.
FRONTIERS IN IMMUNOLOGY
(2022)
Correction
Multidisciplinary Sciences
Laszlo Gyongyosi, Sandor Imre
Summary: The paper has been amended and the updated version can be accessed through the link at the top of the paper.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Qinqin Peng, Ke Sha, Yingzi Liu, Mengting Chen, San Xu, Hongfu Xie, Zhili Deng, Ji Li
Summary: The study found that angiogenesis was enhanced in both rosacea patients and LL37-induced rosacea-like mice, with inhibition of angiogenesis alleviating rosacea-like features. Activation of mTORC1 in endothelial cells was observed in the lesional skin, and inhibiting mTORC1 decreased angiogenesis and blocked rosacea development. In vitro results showed that inhibiting mTORC1 signaling significantly decreased LL37-induced tube formation of human endothelial cells, suggesting that targeting angiogenesis may be a potential therapy for rosacea.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Mengwen Hu, Yu-Han Yeh, Yasuhisa Munakata, Hironori Abe, Akihiko Sakashita, So Maezawa, Miguel Vidal, Haruhiko Koseki, Neil Hunter, Richard M. Schultz, Satoshi H. Namekawa
Summary: The Polycomb Repressive Complex 1 (PRC1) is crucial for the formation and maintenance of the ovarian reserve, which is essential for the female reproductive lifespan. Through establishing repressive chromatin states, PRC1 suppresses the gene expression program of meiotic prophase-I, enabling the transition to dictyate arrest and preventing the depletion of the ovarian reserve.
NATURE COMMUNICATIONS
(2022)
Correction
Mathematics
John R. Klein, Cary Malkiewich
Summary: We point out an error in the authors' earlier proof of the functoriality of the Becker-Gottlieb transfer. Due to this error, the question of functoriality is once again open.
ADVANCES IN MATHEMATICS
(2022)
Article
Biochemistry & Molecular Biology
Michael Chute, Preetinder K. Aujla, Yingxi Li, Sayantan Jana, Pavel Zhabyeyev, Jaslyn Rasmuson, Caroline A. Owen, Thomas Abraham, Gavin Y. Oudit, Zamaneh Kassiri
Summary: ADAM15 plays an important role in heart disease, and its deficiency can lead to impaired scar formation after myocardial infarction, potentially related to collagen cross-linking and kinase expression.
Article
Biotechnology & Applied Microbiology
Katarzyna Bogucka, Federico Marini, Sebastian Rosigkeit, Janine Schloeder, Helmut Jonuleit, Kerstin David, Margarita Schlackow, Krishnaraj Rajalingam
Summary: KRAS is frequently mutated in lung cancers, making it a prime target for treatment. This study reveals that ERK3 plays a necessary role in KRAS-mediated NSCLC tumors, with its kinase activity required for KRAS-driven oncogenesis. Inhibitors targeting ERK3 kinase may be pursued for treating KRAS G12C-driven tumors.
CANCER GENE THERAPY
(2021)
Article
Plant Sciences
Gang Yu, Liu Xian, Haiyan Zhuang, Alberto P. Macho
Summary: The research indicates that SGT1 does not play a significant role in bacterial PAMP-triggered immunity, and is not limiting for early PRR-dependent responses or antibacterial immunity.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Plant Sciences
Reinhold Stockenhuber, Reiko Akiyama, Nicolas Tissot, Stefan Milosavljevic, Misako Yamazaki, Michele Wyler, Adriana B. Arongaus, Roman Podolec, Yasuhiro Sato, Alex Widmer, Roman Ulm, Kentaro K. Shimizu
Summary: This study investigates the impact of UV-B radiation on plant fitness and gene expression. The results show that the UV-B photoreceptor UVR8 plays an important role in plant acclimation and tolerance to UV-B stress. Additionally, the study identifies other genes and pathways involved in oxidative stress, photoprotection, and DNA damage repair in response to UV-B radiation. These findings highlight the functional importance of UVR8-mediated response in plants.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Qin Xia, Jose C. Casas-Martinez, Eduardo Zarzuela, Javier Munoz, Antonio Miranda-Vizuete, Katarzyna Goljanek-Whysall, Brian McDonagh
Summary: Exercise increases Reactive Oxygen Species (ROS) in muscle, promoting changes in gene transcription and mitochondrial biogenesis. Peroxiredoxin 2 (Prdx2) is necessary for the adaptive response to physiological levels of H2O2 and exercise. H2O2 improves mitochondrial capacity and myogenesis in myoblasts, which is suppressed when Prdxs expression is decreased. Swimming exercise in C. elegans enhances mitochondrial content, fitness, survival, and longevity, but these benefits are diminished in prdx-2 mutant worms. Prdx-2 plays a key regulatory role in the redox signalling cascade following endogenous ROS generation.
Article
Biochemistry & Molecular Biology
Sanam Maikoo, Abir Chakraborty, Nyeleti Vukea, Laura Margaret Kirkpatrick Dingle, William John Samson, Jo-Anne de la Mare, Adrienne Lesley Edkins, Irvin Noel Booysen
Summary: The interactions of ruthenium heterocyclic complexes with DNA and BSA were studied using UV-Vis spectrophotometry, fluorescence emission spectroscopy, and agarose gel electrophoresis. The complexes were found to be groove-binders with high DNA binding constants. Additionally, they exhibited high radical scavenging activities and moderate cytotoxicity against cancer cell lines.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Review
Chemistry, Medicinal
Blessing Mabate, Chantal Desiree Daub, Samkelo Malgas, Adrienne Lesley Edkins, Brett Ivan Pletschke
Summary: Fucoidans are complex polysaccharides derived from brown seaweeds with reported bioactive characteristics such as antidiabetic and anticancer properties. However, the understanding of the structure and biological activity of fucoidan remains unclear. Limited data exists on the dual chemotherapeutic efforts for the link between diabetes and cancer.
Review
Cell Biology
Patricija van Oosten-Hawle, Steven Bergink, Brian Blagg, Jeff Brodsky, Adrienne Edkins, Brian Freeman, Olivier Genest, Linda Hendershot, Harm Kampinga, Jill Johnson, Antonio De Maio, Dan Masison, Kevin Morano, Gabriele Multhoff, Chris Prodromou, Veena Prahlad, Ruth Scherz-Shouval, Anastasia Zhuravleva, Mehdi Mollapour, Andrew W. Truman
Summary: The CSSI organized a virtual meeting on November 5-6, 2020, aiming to provide an international platform for scientists in the field of cell stress and chaperones to exchange data and ideas during the Covid-19 pandemic.
CELL STRESS & CHAPERONES
(2021)
Article
Biochemistry & Molecular Biology
Mziyanda Mbaba, Laura M. K. Dingle, Ayanda I. Zulu, Dustin Laming, Tarryn Swart, Jo-Anne de la Mare, Heinrich C. Hoppe, Adrienne L. Edkins, Setshaba D. Khanye
Summary: A series of tailored coumarin-based ferrocenyl 1,3-oxazine hybrid compounds were synthesized and found to exhibit higher in vitro antiparasitic potency against malaria and trypanosomiasis. Structure-activity relationship analyses suggest that the presence of the coumarin nucleus is tolerated for biological activity but may lead to reduced efficacy. Preliminary mechanistic studies with the most promising compound support hemozoin inhibition and DNA interaction as potential mechanisms for producing plasmocidal and antitrypanosomal effects.
Article
Pharmacology & Pharmacy
Serah Kimani, Suparna Chakraborty, Ikponmwosa Irene, Jo de la Mare, Adrienne Edkins, Andre du Toit, Ben Loos, Angelique Blanckenberg, Annick Van Niekerk, Leticia Costa-Lotufo, K. N. ArulJothi, Selwyn Mapolie, Sharon Prince
Summary: The study investigated the anti-cancer activity of a palladium complex, BTC2, in human breast cancer cells, demonstrating its selectivity, inhibition of cancer cell migration, and suppression of cancer stem cell activity. BTC2 also induced DNA double strand breaks, triggered cell cycle arrests, and activated multiple cell death pathways, showing promising anti-breast cancer activity both in vitro and in vivo.
BIOCHEMICAL PHARMACOLOGY
(2021)
Editorial Material
Biochemistry & Molecular Biology
Chen Lior, Francesca Hodge, Evandro A. De-Souza, Dimitra Bourboulia, Stuart K. Calderwood, Della David, D. Allan Drummond, Adrienne Edkins, Richard I. Morimoto, Veena Prahlad, Oded Rechavi, Lea Sistonen, Mark Wilson, R. Luke Wiseman, Maurizio Zanetti, Rebecca Taylor, Ruth Scherz-Shouval, Patricija van Oosten-Hawle
Article
Chemistry, Multidisciplinary
Songeziwe Ntsimango, Sendibitiyosi Gandidzanwa, Sinelizwi V. Joseph, Eric C. Hosten, Marvin Randall, Adrienne L. Edkins, Samson M. Khene, Philani Mashazi, Tebello Nyokong, Abubak'r Abrahams, Zenixole R. Tshentu
Summary: A novel method of accessing rhenium(V)-phthalocyanine complexes through metalation and subsequent nanoparticle synthesis has potential for tumor targeting applications.
Review
Biochemistry & Molecular Biology
Tanima Dutta, Harpreet Singh, Adrienne L. Edkins, Gregory L. Blatch
Summary: Heat shock protein 90 (Hsp90) plays an important role in cellular protein homeostasis. In parasitic protozoans, such as Plasmodium falciparum, Hsp90 and its associated co-chaperones have unique properties and functions. Understanding these differences may lead to the development of drugs to combat malaria.
Article
Biochemistry & Molecular Biology
Michaelone C. Vaaltyn, Maria Mateos-Jimenez, Ronel Muller, C. Logan Mackay, Adrienne L. Edkins, David J. Clarke, Clinton G. L. Veale
Summary: Contemporary medicinal chemistry considers fragment-based drug discovery (FBDD) and inhibition of protein-protein interactions (PPI) as important means of expanding the volume of druggable chemical space. This study utilized the speed and sensitivity of nanoelectrospray (nano-ESI) native mass spectrometry to identify fragments that bind to the TPR2AB domain of HOP. The fragments were validated through biophysical assessment, demonstrating binding and inhibitory activity against the TPR2A domain and the HSP90 C-terminal domain.
Article
Biochemistry & Molecular Biology
Miebaka Jamabo, Stephen John Bentley, Paula Macucule-Tinga, Praise Tembo, Adrienne Lesley Edkins, Aileen Boshoff
Summary: This study provides an in silico overview of HSP90 and its co-chaperones in African trypanosomes. Structural and phylogenetic analysis revealed differences in HSP90 proteins between different trypanosome species. The study also identified putative HSP90 genes and co-chaperones, providing an updated framework for drug target research in African trypanosomes.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Cell Biology
Patricija van Oosten-Hawle, Sarah J. Backe, Anat Ben-Zvi, Dimitra Bourboulia, Mara Brancaccio, Jeff Brodsky, Melody Clark, Giorgio Colombo, Marc B. Cox, Paolo de los Rios, Frank Echtenkamp, Adrienne Edkins, Brian Freeman, Pierre Goloubinoff, Walid Houry, Jill Johnson, Paul LaPointe, Wei Li, Valerie Mezger, Len Neckers, Nadinath B. Nillegoda, Veena Prahlad, Adam Reitzel, Ruth Scherz-Shouval, Lea Sistonen, Francis T. F. Tsai, Mark R. Woodford, Mehdi Mollapour, Andrew W. Truman
Summary: The Second International Symposium on Cellular and Organismal Stress Responses was held virtually on September 8-9, 2022. Supported by the Cell Stress Society International (CSSI), the symposium was organized by Patricija Van OostenHawle and Andrew Truman from the University of North Carolina at Charlotte, USA, and Mehdi Mollapour from SUNY Upstate Medical University, USA. The goal of the symposium was to provide a platform for researchers and students to exchange ideas on cellular stress and chaperones. This article will summarize the meeting highlights and acknowledge CSSI's recognition of outstanding contributors.
CELL STRESS & CHAPERONES
(2023)
Editorial Material
Biochemistry & Molecular Biology
Clinton G. L. Veale, Temitope O. Olomola, Prinessa Chellan, Adrienne L. Edkins
Summary: The young, fast-growing population of Africa means that utilizing the economic benefits of scientific research is critical to sustainable and equitable growth on the continent. The whole world would also benefit from the intellectual contributions that would come from supporting African science. Chemical biology, particularly in relation to neglected diseases in Africa, is considered an important field. This editorial examines the convergence of science between chemistry and biology in an African context, its global significance, and the factors that both support and hinder African scientific contributions. The Biological and Medicinal Chemistry in Africa special collection showcases the diverse range of African chemical biology.
Article
Biochemistry & Molecular Biology
Victor Barozi, Adrienne L. Edkins, Ozlem Tastan Bishop
Summary: This study systematically characterized the effects of mutations in the Omicron sub-lineages on the interaction between the viral S protein and hACE2, and found that these mutations may contribute to antibody escape and alter conformational flexibility.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Clinton G. L. Veale, Maria Mateos-Jimenez, Michaelone C. Vaaltyn, Ronel Muller, Matodzi P. Makhubu, Mahama Alhassan, Beatriz G. de la Torre, Fernando Albericio, C. Logan Mackay, Adrienne L. Edkins, David J. Clarke
Summary: This study introduces a native mass spectrometry protein-peptide model as a suitable replacement for HOP-HSP90 protein-protein interaction, leading to the identification of two new peptides capable of disrupting PPI in vitro. It demonstrates a viable alternative for screening PPI inhibitors.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Shane Patrick Flanagan, Ronen Fogel, Adrienne Lesley Edkins, Lance St. John Ho, Janice Limson
Summary: Most aptamers targeting cell-expressed antigens are generated in vitro against synthetic oligopeptide epitopes or recombinant proteins, leading to nonspecific binding due to the lack of mimicry of the in vivo environment. The presence of dead cells and cellular debris complicates aptamer targeting by increasing nonspecific affinities. Evaluation of cell viability is rarely applied in in vivo testing of aptamer binding, with little understanding of how dead cells uptake existing aptamers.
ANALYTICAL METHODS
(2021)
