Article
Polymer Science
Malene Moller Jorgensen, Jenni Kathrine Sloth, Rikke Baek
Summary: The study on extracellular vesicles (EVs) emphasizes the importance of optimization experiments when characterizing EVs in a clinical setting. Factors such as storage conditions and additives have a significant impact on the results of EV Array analysis.
Article
Biochemical Research Methods
Raghu Ramanathan, Nathan A. Delvaux, Kevin G. Rice
Summary: We developed an improved in vitro transfection assay for testing the efficiency of non-viral vector DNA nanoparticle transfection of primary hepatocytes. The assay was optimized for cell plating number, DNA dose, and PEI to DNA ratio, and provides reliable in vitro expression results for direct comparison of different non-viral gene delivery vectors.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Soojung Lee, Jonathan Chang, Sung-Min Kang, Eric Parigoris, Ji-Hoon Lee, Yun Suk Huh, Shuichi Takayama
Summary: This manuscript presents a new method for forming basal-in MCF10A organoids using commercial 384-well ultra-low attachment microplates. The development of associated live-cell imaging and automated analysis protocols allows for improved tracking of organoid formation and growth. The results and analysis protocol described in this study can serve as a practical resource for future research in this area.
SCIENTIFIC REPORTS
(2022)
Article
Biochemical Research Methods
Shilpaa Mukundan, Jordan Bell, Matthew Teryek, Charles Hernandez, Andrea C. Love, Biju Parekkadan, Leo Li-Ying Chan
Summary: Tumor spheroid models are important for studying cancer cell responses to chemotherapy. This study developed a breast cancer spheroid image cytometry assay that allows for convenient and high-throughput drug-response testing. The assay uses an automated imaging system to track and analyze thousands of spheroids and employs fluorescent staining to study the effects of anti-cancer drugs. The results show that spheroid size reduction correlates with cell viability, and the method can serve as a foundation for studying other three-dimensional spheroids and tissue samples.
JOURNAL OF FLUORESCENCE
(2022)
Article
Biochemical Research Methods
Janis H. Doss, Nazir Barekzi, David T. Gauthier
Summary: Bacteriophages have diverse applications in various fields and it is important to enhance the efficiency and economy of phage discovery. This study focused on the procedures of enrichment, isolation, detection, and purification of phages using Mycobacterium smegmatis mc2155 as a model host. The research demonstrated successful enrichment and detection techniques, as well as a method for purifying single phage types from mixed cultures. These multi-well techniques can be alternative or complementary approaches to traditional methods of phage discovery and characterization.
JOURNAL OF MICROBIOLOGICAL METHODS
(2022)
Article
Biochemistry & Molecular Biology
Dimitrios Baltzis, George Z. Tsogas, Constantinos K. Zacharis, Paraskevas D. Tzanavaras
Summary: A high-throughput fluorimetric assay for histidine was developed using a 96-well plates platform. The method detects histidine in human urine samples using a smartphone after illumination under UV light without the need for instruments. The results were in good agreement with an HPLC corroborative method, and the assay showed minimal interference from other substances in urine.
Article
Pharmacology & Pharmacy
Lena C. Preiss, Volker M. Lauschke, Katrin Georgi, Carl Petersson
Summary: In this study, multi-well spheroids of primary human hepatocytes were compared to suspension and single spheroid cultures. The multi-well spheroids showed sustained physiological function and metabolic activity, resulting in significant turnover of low clearance compounds. Compared to single spheroid or suspension cultures, multi-well spheroids provided more accurate predictions of clearance.
Article
Biochemical Research Methods
Michaela Feodoroff, Piia Mikkonen, Mariliina Arjama, Astrid Murumagi, Olli Kallioniemi, Swapnil Potdar, Laura Turunen, Vilja Pietiainen
Summary: We established a detailed protocol for the 3D drug testing of patient-derived cancer cells (PDCs) using Matrigel in a 384-well plate format. An alternative protocol without supporting matrices was also provided. The 3D-DSRT method can be performed within 1-3 weeks and can be flexibly modified for different cell models.
Article
Marine & Freshwater Biology
Ines L. Pereira, Celia Lopes, Eduardo Rocha, Tania V. Madureira
Summary: This study optimized a 3D model of brown trout hepatocyte spheroids and used it to investigate the effects of androgens on lipidic target genes. The results suggest that DHT modulates distinct lipidic pathways in brown trout and demonstrate that this 3D model is a valuable tool for physiological and toxicological mechanistic studies.
AQUATIC TOXICOLOGY
(2022)
Article
Toxicology
Andrea Rowan-Carroll, Anthony Reardon, Karen Leingartner, Remi Gagne, Andrew Williams, Matthew J. Meier, Byron Kuo, Julie Bourdon-Lacombe, Ivy Moffat, Richard Carrier, Andy Nong, Luigi Lorusso, Stephen S. Ferguson, Ella Atlas, Carole Yauk
Summary: This study focused on four model PFAS and found that they may have common molecular targets and toxicities, with PFOS and PFDS being the most similar. The data from the study provide baseline toxicity levels for comparison with other known PFAS.
TOXICOLOGICAL SCIENCES
(2021)
Review
Cell & Tissue Engineering
Run-Tong Lian, An-Qi Zhuang, Jing Yue, Yan Chen, Kui-Fen Ma, Yi-Hang Wu
Summary: Hepatic disease is a global health problem and liver transplantation is the most effective treatment, but the shortage of healthy liver donors limits this process. Liver tissue engineering using seeding cells, extracellular matrix, and bioreactors is being explored as an alternative. Hepatocyte-based tissue engineering can overcome shortages in tissue repair, but generating high-quality hepatocyte-like cells (HLCs) for clinical therapy is a challenge. Maintaining the functionality of primary hepatocytes and enhancing liver-specific functions of HLCs are also challenges. Nevertheless, hepatocyte-based liver regenerative medicine is an attractive strategy with some challenges.
CURRENT STEM CELL RESEARCH & THERAPY
(2023)
Article
Chemistry, Analytical
Anna Bach, Heidi Fleischer, Kerstin Thurow
Summary: Therapeutic antibodies are of great interest in the treatment of infectious diseases, autoimmune diseases, and cancer. This article compares miniaturized chromatographic columns and 96-well filter plates for the purification of an IgG antibody in terms of quality, cost, and sustainability. The results show that both methods are highly effective, with minor differences in yield, and the miniaturized chromatographic columns offer the lowest cost per sample. Additionally, the use of automation system enhances employee safety and reduces laboratory waste, indicating increased sustainability.
Article
Pathology
Thuy Phan, Ngoc Yen Kim Tran, Thomas Gottlieb, Steven Siarakas, Genevieve McKew
Summary: During the COVID-19 pandemic, sample pooling has been proven effective in increasing testing capacity and reducing reagent usage. This study evaluated the performance of influenza and RSV targets in the AusDiagnostics SARS-CoV-2, Influenza and RSV 8-well assay and investigated the impact of sample pooling on target detection. The results showed that sample pooling can improve testing efficiency and reduce reagent consumption.
Article
Peripheral Vascular Disease
Mahesh M. Umapathysivam, Bethany Morgan, Carmen Bischoff, Annabelle Hayes, Michael Wilks, Michael Stowasser, David J. Torpy
Summary: Primary aldosteronism is a common cause of secondary hypertension. Surgical treatment (adrenalectomy) is superior to medical management, leading to better cardiovascular outcomes and reduced reliance on medication. Adrenal vein sampling (AVS) is necessary to determine the localization of aldosterone secretion before adrenalectomy.
JOURNAL OF HUMAN HYPERTENSION
(2023)
Article
Health Care Sciences & Services
Lusine Poghosyan, Allison A. Norful, Affan Ghaffari, Jianfang Liu
Summary: The study evaluates the psychometric properties of a survey tool measuring omissions in primary care, revealing four domains of omissions through exploratory factor analysis. Revised subscales indicate acceptable internal consistency reliability and potential for identifying critical omissions in primary care.
JOURNAL OF PATIENT SAFETY
(2021)
Article
Engineering, Biomedical
Zhihua Li, Zijian Chen, Yanan Gao, Yi Xing, Yuping Zhou, Yucheng Luo, Weihong Xu, Zhengchang Chen, Xu Gao, Kapish Gupta, Karthic Anbalakan, Lei Chen, Chuang Liu, Jian Kong, Hwa Liang Leo, Chengzhi Hu, Hanry Yu, Qiongyu Guo
Summary: A new type of magnetic micro-anchor, SM(2)A, has been developed for magnetic guided endovascular localization. It demonstrates stable anchoring in the desired site and produces the necessary action. This micro-anchor can be a promising platform for precision treatment and diagnosis.
Article
Engineering, Biomedical
Yucheng Luo, Yutao Ma, Zijian Chen, Yanan Gao, Yuping Zhou, Xiaoya Liu, Xuezhe Liu, Xu Gao, Zhihua Li, Chuang Liu, Hwa Liang Leo, Hanry Yu, Qiongyu Guo
Summary: Particulate embolic agents with calibrated sizes have gained significant interest in therapeutic embolotherapies. In this study, shape-anisotropic microembolics based on PVA were developed using a simple fabrication method. The embolization behaviors of these nonspherical microembolics were evaluated in vitro and in vivo models. The results demonstrated that compared to microspheres, the elongated microembolics displayed superior endovascular navigation capability, penetration depth, and embolization stability.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Multidisciplinary Sciences
Yi-An Yang, Emmanuelle Nguyen, Gautham Hari Narayana Sankara Narayana, Melina Heuze, Chaoyu Fu, Hanry Yu, Rene-Marc Mege, Benoit Ladoux, Michael P. Sheetz
Summary: E-cadherin is a key molecule involved in mechanotransduction between cells in tissues. Epithelial cells are able to test and respond to the mechanical properties of neighboring cells through contractions of E-cadherin adhesions. This process requires the involvement of multiple proteins.
Article
Chemistry, Analytical
Xiaolin Wu, Cheryl Chan, Stacy L. Springs, Yie Hou Lee, Timothy K. Lu, Hanry Yu
Summary: WS-RADICA is a new nucleic acid detection method that enables rapid, sensitive, and quantitative detection of nucleic acids. It has a lower detection limit and higher inhibitor tolerance, and has been validated for nucleic acids derived from live viruses.
ANALYTICA CHIMICA ACTA
(2022)
Article
Microbiology
Tiantian Zhang, Tianqi Cui, Yaning Cao, Yongzhen Li, Fenghui Li, Derui Zhu, Jiangwa Xing
Summary: In this study, we reported the whole genome of a novel Halomonas species strain XH36 with high ectoine production potential. The analysis showed that XH36 belongs to a novel Halomonas species named Halomonas qaidamensis. Ectoine was the main compatible solute in XH36 and it accumulated ectoine to cope with salt stress. Our findings suggest that XH36 is a promising industrial strain for ectoine production and the genomic analysis provides insights into its salt-induced osmoadaptation mechanisms.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2022)
Article
Physics, Applied
Yan Kong, Shujian Ong, Mei Hui Liu, Hanry Yu, Dejian Huang
Summary: This study successfully fabricated edible microbeads using food-grade sodium alginate and enhanced their cytoaffinity with gelatin coatings. The modified microbeads have self-fluorescence and can be used as microcarriers for cell culture and meat production.
JOURNAL OF PHYSICS D-APPLIED PHYSICS
(2022)
Article
Food Science & Technology
Sean Chia, Gavin Teo, Shi Jie Tay, Larry Sai Weng Loo, Corrine Wan, Lyn Chiin Sim, Hanry Yu, Ian Walsh, Kuin Tian Pang
Summary: This study utilized a glycomic approach to profile meat from different species, revealing distinct glycan profiles and significant differences in glycosylation attributes. By analyzing multi-attribute data, meat from different species could be clearly separated, demonstrating the potential of glycomics-based workflows for product authentication in various industries.
Article
Engineering, Environmental
Tao Yang, Dong Li, Yuhua Yan, Fatima-ezzahra Ettoumi, Ricardo A. Wu, Zisheng Luo, Hanry Yu, Xingyu Lin
Summary: In this study, a hydrogel-mediated RT-LAMP method was developed for rapid and accurate quantification of SARS-CoV-2 virus. The technique offers fast, simple, and highly sensitive detection, and can be used for monitoring cold chain foods.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Materials Science, Biomaterials
Yanan Gao, Jingyu Xiao, Zijian Chen, Yutao Ma, Xiaoya Liu, Dishuang Yang, Hwa Liang Leo, Hanry Yu, Jian Kong, Qiongyu Guo
Summary: Developing a three-dimensional in vitro tumor model with vasculature systems for testing endovascular interventional therapies is challenging. Here, an orthotopic liver tumor spheroid model is developed to evaluate transcatheter arterial chemoembolization (TACE) treatment. The model resembles the unique tumor microenvironment and can efficiently assess various diagnostic and therapeutic strategies.
BIOMATERIALS SCIENCE
(2023)
Review
Cell & Tissue Engineering
Kuin Tian Pang, Larry Sai Weng Loo, Sean Chia, Francesca Yi Teng Ong, Hanry Yu, Ian Walsh
Summary: Stem cells have the unique ability to differentiate into specialized cell types, and skeletal muscle stem cells (MuSCs) play important roles in the growth, repair, and regeneration of skeletal muscle tissues. However, the differentiation, proliferation, and expansion processes of MuSCs face significant challenges, and the molecular role of mechanobiology in MuSC growth, proliferation, and differentiation is poorly understood. This review comprehensively summarizes the impact of different mechanical cues on stem cell growth, proliferation, differentiation, and their potential role in disease development.
STEM CELL RESEARCH & THERAPY
(2023)
Review
Gastroenterology & Hepatology
Kapish Gupta
Summary: Hepatocytes produce and secrete bile components into bile canaliculi, which are formed by the apical membranes of hepatocytes. Bile canaliculi merge to form tubular structures that connect to bile ducts and enable bile flow through the small intestine. Functional requirements of bile canaliculi include maintaining canalicular shape and regulating bile flow, which are mediated by functional modules such as transporters, cytoskeleton, cell-cell junctions, and mechanosensing proteins. Cholestasis, abnormal bile flow, can occur due to dysregulation of these functional modules. This article discusses the interactions and regulation of these modules in bile canaliculi and provides a perspective on recent studies of bile canalicular dynamics.
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Zixuan Zhao, Xinyi Chen, Anna M. Dowbaj, Aleksandra Sljukic, Kaitlin Bratlie, Luda Lin, Eliza Li Shan Fong, Gowri Manohari Balachander, Zhaowei Chen, Alice Soragni, Meritxell Huch, Yi Arial Zeng, Qun Wang, Hanry Yu
Summary: Organoids are simple tissue-engineered cell-based models that mimic the complex structure and function of in vivo tissues. They have a wide range of applications in developmental studies, disease modeling, and personalized medicine. Various engineering strategies have been developed to support their culture, growth, differentiation, and maturation.
NATURE REVIEWS METHODS PRIMERS
(2022)
Meeting Abstract
Immunology
I. E. M. de Jong, M. L. Hunt, K. Gupta, J. Llewellyn, Y. Du, A. P. Dhand, J. W. Gaynor, R. J. Porte, R. G. Wells
Meeting Abstract
Gastroenterology & Hepatology
Iris de Jong, Mallory Hunt, Kapish Gupta, Yu Du, Jessica Llewellyn, Abhishek Dhand, Robert Porte, William Gaynor, Rebecca Wells
JOURNAL OF HEPATOLOGY
(2022)