Multistable and dynamic CRISPRi-based synthetic circuits

A CRISPR/Cas9-based central processing unit to program complex logic computation in human cells

(2019) Hyojin Kim et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Framework for the Modular and Combinatorial Assembly of Synthetic Gene Circuits

(2019) Javier Santos-Moreno et al.   ACS Synthetic Biology

Visualisation of dCas9 target search in vivo using an open-microscopy framework

(2019) Koen J. A. Martens et al.   Nature Communications

Designing cell function: assembly of synthetic gene circuits for cell biology applications

(2018) Mingqi Xie et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Fluorescent guide RNAs facilitate development of layered Pol II driven CRISPR circuits

(2018) David Menn et al.   ACS Synthetic Biology

Kinetics of dCas9 target search inEscherichia coli

(2017) Daniel Lawson Jones et al.   SCIENCE

Digital logic circuits in yeast with CRISPR-dCas9 NOR gates

(2017) Miles W. Gander et al.   Nature Communications

Crosstalk between Diverse Synthetic Protein Degradation Tags in Escherichia coli

(2017) Nicholas C. Butzin et al.   ACS Synthetic Biology

CRISPR-Cas9 nuclear dynamics and target recognition in living cells

(2016) Hanhui Ma et al.   JOURNAL OF CELL BIOLOGY

Synchronous long-term oscillations in a synthetic gene circuit

(2016) Laurent Potvin-Trottier et al.   NATURE

Complex transcriptional modulation with orthogonal and inducible dCas9 regulators

(2016) Yuchen Gao et al.   NATURE METHODS

Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible hybrid T7-lac promoters for modular, tuneable control of metabolic pathway fluxes inEscherichia coli

(2016) Brady F. Cress et al.   NUCLEIC ACIDS RESEARCH

Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology

(2016) Barbara Jusiak et al.   TRENDS IN BIOTECHNOLOGY

A renaissance in RNA synthetic biology: new mechanisms, applications and tools for the future

(2015) James Chappell et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Cas9 gRNA engineering for genome editing, activation and repression

(2015) Samira Kiani et al.   NATURE METHODS

Orthogonal Modular Gene Repression in Escherichia coli Using Engineered CRISPR/Cas9

(2015) Andriy Didovyk et al.   ACS Synthetic Biology

Rapid cell-free forward engineering of novel genetic ring oscillators

(2015) Henrike Niederholtmeyer et al.   eLife

Broccoli: Rapid Selection of an RNA Mimic of Green Fluorescent Protein by Fluorescence-Based Selection and Directed Evolution

(2014) Grigory S. Filonov et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Multiplexed and Programmable Regulation of Gene Networks with an Integrated RNA and CRISPR/Cas Toolkit in Human Cells

(2014) Lior Nissim et al.   MOLECULAR CELL

Multi-input CRISPR/Cas genetic circuits that interface host regulatory networks

(2014) A. A. Nielsen et al.   Molecular Systems Biology

Dimeric CRISPR RNA-guided FokI nucleases for highly specific genome editing

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

CRISPR transcriptional repression devices and layered circuits in mammalian cells

(2014) Samira Kiani et al.   NATURE METHODS

A bistable genetic switch based on designable DNA-binding domains

(2014) Tina Lebar et al.   Nature Communications

A unified design space of synthetic stripe-forming networks

(2014) Yolanda Schaerli et al.   Nature Communications

An improved Escherichia coli strain to host gene regulatory networks involving both the AraC and LacI inducible transcription factors

(2014) Manjunatha Kogenaru et al.   Journal of Biological Engineering

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

(2013) David Bikard et al.   NUCLEIC ACIDS RESEARCH

One-pot DNA construction for synthetic biology: the Modular Overlap-Directed Assembly with Linkers (MODAL) strategy

(2013) Arturo Casini et al.   NUCLEIC ACIDS RESEARCH

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

NUPACK: Analysis and design of nucleic acid systems

(2010) Joseph N. Zadeh et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

Sequence- and Structure-Specific RNA Processing by a CRISPR Endonuclease

(2010) Rachel E. Haurwitz et al.   SCIENCE

Emergent bistability by a growth-modulating positive feedback circuit

(2009) Cheemeng Tan et al.   Nature Chemical Biology