Article
Biochemistry & Molecular Biology
Takataro Fukuhara, Yoshihiro Ueda, Sung-Il Lee, Tokifumi Odaka, Shinsuke Nakajima, Jun-Ichi Fujisawa, Kazu Okuma, Makoto Naganuma, Kazuichi Okazaki, Naoyuki Kondo, Yuji Kamioka, Mitsuru Matsumoto, Tatsuo Kinashi
Summary: In humanized mice, immunization leads to an increase in HLA-DR+ cells, including CD205(+) dendritic cells, which promotes HLA-restricted thymocyte expansion. This finding offers a unique opportunity to generate humanized mice with ease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Andres Garcia-Garcia, Thibaut Klein, Gordian Born, Morgane Hilpert, Arnaud Scherberich, Claudia Lengerke, Radek C. Skoda, Paul E. Bourgine, Ivan Martin
Summary: Human malignant hematopoietic stem and progenitor cells reside in bone marrow niches, and in vitro systems have been developed to mimic these niches for studying leukemia and drug responses.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Immunology
Ryutaro Iwabuchi, Keigo Ide, Kazutaka Terahara, Ryota Wagatsuma, Rieko Iwaki, Hiroko Matsunaga, Yasuko Tsunetsugu-Yokota, Haruko Takeyama, Yoshimasa Takahashi
Summary: The study reveals that CD14(+)CD1c(+) cells in humanized mouse models are phenotypically distinct from cDC2s, indicating their identity as a subset equivalent to DC3s.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Sophia Giang, David A. Horwitz, Sean Bickerton, Antonio La Cava
Summary: This study introduces a method to produce acellular tolerogenic aAPCs using PLGA nanoparticles, which can induce human T cells to become functional Tregs and modulate systemic autoimmunity. It highlights the potential of using PLGA NPs as a targeted approach to repair IL-2 and/or TGF-beta defects in autoimmune diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Can M. Sungur, Qiankun Wang, Ayse N. Ozanturk, Hongbo Gao, Aaron J. Schmitz, Marina Cella, Wayne M. Yokoyama, Liang Shan
Summary: The role of NK cells in HIV-1 infections was investigated using a humanized mouse model. The study showed that NK cells directly provided anti-HIV-1 responses in nonlymphoid organs, but had limited functionality in lymphoid organs. Antiretroviral therapy and a broadly neutralizing antibody, PGT121, were found to enhance NK cell function and reduce viral load.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Biotechnology & Applied Microbiology
Carine Bouffi, Kathryn A. Wikenheiser-Brokamp, Praneet Chaturvedi, Nambirajan Sundaram, Gillian R. Goddard, Mark Wunderlich, Nicole E. Brown, Janet F. Staab, Rachel Latanich, Nicholas C. Zachos, Emily M. Holloway, Maxime M. Mahe, Holly M. Poling, Simon Vales, Garrett W. Fisher, Jason R. Spence, James C. Mulloy, Aaron M. Zorn, James M. Wells, Michael A. Helmrath
Summary: Human intestinal organoids (HIOs) derived from pluripotent stem cells were transplanted into mice with a humanized immune system to incorporate immune cells. The transplanted HIOs exhibited migration and formation of cellular aggregates similar to human intestinal lymphoid follicles. Upon microbial exposure, the HIOs showed increased epithelial microfold cells and immune cell activation through IgA antibody secretion, providing a useful model for studying infection- or allergen-driven intestinal diseases.
NATURE BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Pernille Kristine Fisker Christensen, Axel Kornerup Hansen, Sren Skov, Britta Cathrina Martel, Jesper Larsen, Maria Helena Hoyer-Hansen, Janne Koch
Summary: In this study, the researchers investigated whether human immune cells played a role in the induction of psoriasis-like skin inflammation in hFlt3L-boosted BRGSF-HIS mice treated with IMQ. The results showed that although the mice exhibited clinical skin inflammation, increased epidermal thickness, and influx of human immune cells, the human immune response was not significant. Instead, the main driving cellular mechanisms were of murine origin, as indicated by the increased number of murine neutrophils and cytokines and chemokines in the skin and systemically after IMQ application.
Article
Immunology
Jianshui Zhang, Saroj Chandra Lohani, Yilun Cheng, Tao Wang, Lili Guo, Woong-Ki Kim, Santhi Gorantla, Qingsheng Li
Summary: This study conclusively demonstrated that human microglia in the adult brain can be reconstituted from HSPCs in mice with human interleukin-34 transgene, supporting HIV-1 infection. This presents a valuable model for studying HIV-1 pathogenesis and evaluating therapeutic approaches.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell & Tissue Engineering
Mohamed A. Ghoneim, Mahmoud M. Gabr, Ayman F. Refaie, Sawsan M. El-Halawani, Mohga M. Al-issawi, Batoul L. Elbassiouny, Mai A. Abd El Kader, Amani M. Ismail, Mona F. Zidan, Mary S. Karras, Raghda W. Magar, Sherry M. Khater, Sylvia A. Ashamallah, Mahmoud M. Zakaria, Malgorzata Kloc
Summary: This study investigated allogenic immune responses following the transplantation of insulin-producing cells (IPCs) differentiated from human adipose tissue-derived stem cells (hAT-MSCs) into humanized mice. The results showed that the engrafted cells were not affected by allogenic immune response, suggesting potential for cell therapy for insulin-dependent diabetes without immunosuppression or encapsulation.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Microbiology
Nnamdi Ikeogu, Oluwaseun Ajibola, Romaniya Zayats, Thomas T. Murooka
Summary: This review discusses the role of memory T cells in HIV infection and how HIV hijacks normal immune processes to establish long-term infection.
Article
Cell & Tissue Engineering
Gordian Born, Marina Nikolova, Arnaud Scherberich, Barbara Treutlein, Andres Garcia-Garcia, Ivan Martin
Summary: The study suggests using stromal vascular fraction derived from human adipose tissue to vascularize 3D osteoblastic bone marrow niches, leading to improved maintenance of HSCs and increased CFU-GEMM numbers. Vascularization also positively affects osteogenic gene expression in the niche.
JOURNAL OF TISSUE ENGINEERING
(2021)
Review
Immunology
Alice Pievani, Roberto Savoldelli, Juliane Poelchen, Elisa Mattioli, Giorgio Anselmi, Alice Girardot, Jochen Utikal, Pierre Bourdely, Marta Serafini, Pierre Guermonprez
Summary: Tissue engineering provides numerous opportunities in regenerative medicine, drug testing, and modeling hematopoiesis. Replicating the physiological microenvironments supporting leukocyte development is crucial for accurately modeling immune cell development. Immune niches are characterized by mesenchymal stromal cells that play unique roles in organizing niche development, maintenance, and function.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Do Hee Kwon, Jae Berm Park, Joo Sang Lee, Sung Joo Kim, Bongkum Choi, Ki-Young Lee
Summary: This study investigated the in vivo functions of hDlk1-expressing MSCs in humanized NSG mice, showing that hDlk1(+)hMSCs can effectively promote the development of human T cells and induce antigen-specific T cell response. The diverse and functionally active human T cells developed in humanized NSG mice suggest potential advantages for developing therapeutics targeting infectious diseases in the future.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Liang Cheng, Guangming Li, Caroline Marnata Pellegry, Fumihiko Yasui, Feng Li, Sandra M. Zurawski, Gerard Zurawski, Yves Levy, Jenny P. -Y. Ting, Lishan Su
Summary: TLR9 and CD40-targeting vaccination can induce human B cell maturation and immunoglobulin class-switch in humanized mice in a pDC-dependent manner, leading to the production of IgG antibodies.
FRONTIERS IN IMMUNOLOGY
(2021)
Editorial Material
Oncology
Karina Silina
Summary: Cytotoxic T cells play a crucial role in fighting against most cancers. In the latest issue of Cancer Cell, Gaglia et al. reveal how tumor tissue architecture changes can create niches for T cell-B cell interactions, which may enhance anti-tumor T cell responses.
Article
Gastroenterology & Hepatology
Sheena Suthen, Chun Jye Lim, Phuong H. D. Nguyen, Charles-Antoine Dutertre, Hannah L. H. Lai, Martin Wasser, Camillus Chua, Tony K. H. Lim, Wei Qiang Leow, Tracy Jiezhen Loh, Wei Keat Wan, Yin Huei Pang, Gwyneth Soon, Peng Chung Cheow, Juinn Huar Kam, Shridhar Iyer, Alfred Kow, Wai Leong Tam, Timothy W. H. Shuen, Han Chong Toh, Yock Young Dan, Glenn K. Bonney, Chung Yip Chan, Alexander Chung, Brian K. P. Goh, Weiwei Zhai, Florent Ginhoux, Pierce K. H. Chow, Salvatore Albani, Valerie Chew
Summary: This study analyzed the immune landscapes of hypoxic regions in hepatocellular carcinoma (HCC) and identified enriched immune subsets, including regulatory T cells, M2 macrophages, and HLA-DRlo cDC2, in hypoxia-high tumor regions. In contrast, hypoxia-low tumor regions showed an abundance of active granzyme B-hi PD-1(lo) CD8(+) T cells, indicating a relatively active immune landscape. The up-regulation of cancer-associated genes in tumor tissues and immunosuppressive genes in tumor-infiltrating leukocytes supported a highly pro-tumorigenic network in hypoxic HCC.
Article
Multidisciplinary Sciences
Florian Ingelfinger, Lisa Ann Gerdes, Vladyslav Kavaka, Sinduya Krishnarajah, Ekaterina Friebel, Edoardo Galli, Pascale Zwicky, Reinhard Furrer, Christian Peukert, Charles-Antoine Dutertre, Klara Magdalena Eglseer, Florent Ginhoux, Andrea Flierl-Hecht, Tania Kumpfel, Donatella De Feo, Bettina Schreiner, Sarah Mundt, Martin Kerschensteiner, Reinhard Hohlfeld, Eduardo Beltran, Burkhard Becher
Summary: This study investigated the influence of genetic predisposition and environmental factors on the peripheral immune signatures in monozygotic twins discordant for multiple sclerosis (MS). Through data-driven computational tools and high-throughput single-cell technologies, the researchers identified an inflammatory shift in a specific group of immune cells in twins with MS, as well as the emergence of certain hyper-responsive immune cells. They found that the variance in CD25 expression by certain helper T cells with a naive phenotype was largely driven by genetic and shared early environmental influences. However, the expansion of helper T cells in twins with MS, which were also elevated in non-twin patients with MS, appeared to be independent of individual genetic makeup and correlated with disease severity.
Review
Biotechnology & Applied Microbiology
Yuan Tian, Lindsay N. Carpp, Helen E. R. Miller, Michael Zager, Evan W. Newell, Raphael Gottardo
Summary: This review summarizes existing studies using single-cell technologies to investigate the immune responses and molecular mechanisms in COVID-19. Understanding the immune cell subsets and molecular factors associated with protective or pathological immunity may contribute to the development of vaccines and therapeutics for COVID-19. Single-cell technologies show promise in uncovering the heterogeneity of immune responses and pathogenesis mechanisms of COVID-19.
NATURE BIOTECHNOLOGY
(2022)
Article
Oncology
Shamin Li, Summer Zhuang, Antja Heit, Si-Lin Koo, Aaron C. Tan, I-Ting Chow, William W. Kwok, Iain Beehuat Tan, Daniel S. W. Tan, Yannick Simoni, Evan W. Newell
Summary: The study reveals the phenotypic heterogeneity of CD4(+) T cells in lung and colorectal cancers, which can also recognize cancer-unrelated antigens.
Article
Biochemistry & Molecular Biology
Rodrigo Nalio Ramos, Yoann Missolo-Koussou, Yohan Gerber-Ferder, Christian P. Bromley, Mattia Bugatti, Nicolas Gonzalo Nunez, Jimena Tosello Boari, Wilfrid Richer, Laurie Menger, Jordan Denizeau, Christine Sedlik, Pamela Caudana, Fiorella Kotsias, Leticia L. Niborski, Sophie Viel, Mylene Bohec, Sonia Lameiras, Sylvain Baulande, Laetitia Lesage, Andre Nicolas, Didier Meseure, Anne Vincent-Salomon, Fabien Reyal, Charles-Antoine Dutertre, Florent Ginhoux, Lene Vimeux, Emmanuel Donnadieu, Benedicte Buttard, Jerome Galon, Santiago Zelenay, William Vermi, Pierre Guermonprez, Eliane Piaggio, Julie Helft
Summary: This study identifies a specific population of FOLR2(+) tissue-resident macrophages in healthy mammary gland and breast cancer primary tumors, which interact with CD8(+) T cells and positively correlate with better patient survival.
Review
Immunology
Florent Ginhoux, Adam Yalin, Charles Antoine Dutertre, Ido Amit
Summary: The immune system is a complex, dynamic, and plastic ecosystem composed of multiple cell types. The advent of single-cell genomic approaches has revolutionized our knowledge of the cellular and molecular makeup of the immune system. Integration of multiomics at the single-cell level will pave the way for future advances in immunology research and clinical translation.
Article
Oncology
Nicholas A. Vitanza, Ashley L. Wilson, Wenjun Huang, Kristy Seidel, Christopher Brown, Joshua A. Gustafson, Jason K. Yokoyama, Adam J. Johnson, Blake A. Baxter, Ryan W. Koning, Aquene N. Reid, Michael Meechan, Matthew C. Biery, Carrie Myers, Stephanie D. Rawlings-Rhea, Catherine M. Albert, Samuel R. Browd, Jason S. Hauptman, Amy Lee, Jeffrey G. Ojemann, Michael E. Berens, Matthew D. Dun, Jessica B. Foster, Erin E. Crotty, Sarah E. S. Leary, Bonnie L. Cole, Francisco A. Perez, Jason N. Wright, Rimas J. Orentas, Tony Chour, Evan W. Newell, Jeffrey R. Whiteaker, Lei Zhao, Amanda G. Paulovich, Navin Pinto, Juliane Gust, Rebecca A. Gardner, Michael C. Jensen, Julie R. Park
Summary: This study reports the first administration of repeatedly dosed intracranial B7-H3 CAR T cells for patients with DIPG, indicating tolerability, detection of CAR T cells in the CSF, elevation of CSF cytokines supporting locoregional immune activation, and the feasibility of serial mass spectrometry from both serum and CSF.
Article
Oncology
Mattia Bugatti, Marco Bergamini, Francesco Missale, Matilde Monti, Laura Ardighieri, Irene Pezzali, Sara Picinoli, Nicoletta Caronni, Yoann Missolo-Koussou, Julie Helft, Federica Benvenuti, William Vermi
Summary: This study reveals the expression of TIM4 on cavity macrophages and macrophages of carcinoma-associated tertiary lymphoid structures in cancer patients, indicating their association with immune-reactive tumors. The two distinct macrophage populations marked by TIM4 exhibit differences in the expression of immunosuppressive molecules and patient prognosis.
CANCER IMMUNOLOGY RESEARCH
(2022)
Article
Immunology
Pierre Bourdely, Luciana Petti, Sokchea Khou, Aida Meghraoui-Kheddar, Roxane Elaldi, Julie Cazareth, Noushine Mossadegh-Keller, Julien Boyer, Michael H. Sieweke, Gilles Poissonnet, Anne Sudaka, Veronique M. Braud, Fabienne Anjuere
Summary: This study investigated the origins, phenotypes, and functions of macrophage subsets in mouse and human skin, as well as in cutaneous squamous cell carcinoma (cSCC). The results showed that autofluorescence can serve as a functional marker for phagocytic macrophages in skin and cSCC.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Maria Magdalena Canali, Melanie Guyot, Thomas Simon, Douglas Daoudlarian, Joelle Chabry, Clara Panzolini, Agnes Petit-Paitel, Nicolas Hypolite, Sarah Nicolas, Pierre Bourdely, Heidy Schmid-Antomarchi, Annie Schmid-Alliana, Javier Soria, Babou Karimdjee Soilihi, Paul Hofman, Armelle Prevost-Blondel, Masashi Kato, Evelyne Mougneau, Nicolas Glaichenhaus, Philippe Blancou
Summary: An enriched environment reduces lung metastasis in mice, potentially through the reprogramming of inflammatory monocytes and modulation of adrenergic receptors and glucocorticoid receptors signaling. This study highlights the complex role of inflammatory monocytes in cancer progression and suggests a novel anti-tumor mechanism associated with environmental factors.
CANCER CELL INTERNATIONAL
(2023)
Article
Immunology
Celestine N. Wanjalla, Curtis L. Gabriel, Hubaida Fuseini, Samuel S. Bailin, Mona Mashayekhi, Joshua Simmons, Christopher M. Warren, David R. Glass, Jared Oakes, Rama Gangula, Erin Wilfong, Stephen Priest, Tecla Temu, Evan W. Newell, Suman Pakala, Spyros A. Kalams, Sara Gianella, David Smith, David G. Harrison, Simon A. Mallal, John R. Koethe
Summary: People with HIV on long-term antiretroviral therapy have a higher risk of cardiometabolic diseases, partly due to persistent inflammation despite viral suppression. Immune responses to co-infections, such as cytomegalovirus, may contribute to these comorbidities and could be targeted for potential therapies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemical Research Methods
Quentin Blampey, Nadege Bercovici, Charles-Antoine Dutertre, Isabelle Pic, Joana Mourato Ribeiro, Fabrice Andre, Paul-Henry Cournede
Summary: Cytometry technology allows for precise single-cell phenotyping within heterogeneous populations. However, traditional manual gating for cell type annotation lacks reproducibility and sensitivity to batch effect. In this study, we introduce Scyan, a novel Single-cell Cytometry Annotation Network that automatically annotates cell types using prior expert knowledge. We demonstrate that Scyan outperforms current state-of-the-art models on multiple public datasets and provides faster and interpretable results. Scyan also addresses complementary tasks such as batch-effect correction, debarcoding, and population discovery.
BRIEFINGS IN BIOINFORMATICS
(2023)
Meeting Abstract
Oncology
Tony Chour, Alexandre Hirayama, Ye Zheng, Alyssa Sheih, Summer Zhuang, Ashley Wilson, Vicky Wu, Raphael Gottardo, Cameron Turtle, Rebecca Gardner, Evan Newell
Meeting Abstract
Oncology
Mahesh Yadav, Michael Fehlings, Leesun Kim, Xiangnan Guan, Kobe C. Yuen, Alireza Tafazzol, Deepali Rishipathak, Shomyesh Sanjabi, Andrew Wallace, Alessandra Nardin, Siming Ma, Ana Milojkovic, Evan Newell, Sanjeev Mariathasan
Meeting Abstract
Oncology
Evan W. Newell