Article
Cell Biology
Hayato Ide, Yukihisa Hayashida, Yusuke V. Morimoto
Summary: The bacterial signaling molecule c-di-GMP is only found in the cellular slime mold Dictyostelium discoideum among eukaryotes. Dictyostelium cells use c-di-GMP to induce differentiation into stalk cells during their transition from a unicellular to a multicellular state. This study used a fluorescent probe to observe the localization of c-di-GMP within Dictyostelium multicellular bodies, and found that cytosolic c-di-GMP concentrations were significantly higher at the tip of the multicellular body during stalk formation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Xiaoli Zeng, Min Huang, Qing-Xue Sun, Ye-Jun Peng, Xiaomei Xu, Yun-Bin Tang, Ju-Yuan Zhang, Yiling Yang, Cheng-Cai Zhang
Summary: This study identified a c-di-GMP receptor called CdgR, which plays a crucial role in regulating cell size in cyanobacteria. CdgR interacts with a global transcription factor DevH and functions through c-di-GMP-mediated signaling. The findings provide insights into the mechanism of c-di-GMP signaling in controlling bacterial cell size.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Plant Sciences
Kangwen Xu, Danyu Shen, Nianda Yang, Shan-Ho Chou, Mark Gomelsky, Guoliang Qian
Summary: This study uncovered the mechanism by which the PilR-PilS two-component system in the soil gammaproteobacterium Lysobacter enzymogenes regulates twitching motility and HSAF synthesis through the c-di-GMP signaling pathway. The findings highlight the coordination between motility and antifungal production in L. enzymogenes, and demonstrate the specificity in c-di-GMP-mediated circuits.
MOLECULAR PLANT PATHOLOGY
(2021)
Review
Microbiology
Zhaoqing Yu, Wei Zhang, He Yang, Shan-Ho Chou, Michael Y. Galperin, Jin He
Summary: The authors review the diversity of sensory domains related to the bacterial CME regulation and specifically discuss those domains that are capable of sensing gaseous or light signals and the mechanisms they use for regulating cellular c-di-GMP levels.
FEMS MICROBIOLOGY REVIEWS
(2023)
Article
Biotechnology & Applied Microbiology
Yi Li, Shuo Han, Yuqi Wang, Mengyuan Qin, Chengjin Lu, Yingke Ma, Wenqing Yang, Jiajia Liu, Xiaohua Xia, Hailei Wang
Summary: This study reveals the positive regulatory effect of autoinducer-2 on the adherence ability of A. veronii through the modulation of c-di-GMP expression. It provides insights into the colonization strategy of this pathogen and may facilitate the control of A. veronii infection in hosts.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Vignesh Narayan Hariharan, Rahul Yadav, Chandrani Thakur, Albel Singh, Renu Gopinathan, Devendra Pratap Singh, Gaurav Sankhe, Vandana Malhotra, Nagasuma Chandra, Apoorva Bhatt, Deepak Kumar Saini
Summary: This study identifies a c-di-GMP sensing sensor histidine kinase PdtaS, which controls cellular growth and global transcriptional regulation by binding to c-di-GMP and perturbing the PdtaS-PdtaR two-component system. The researchers characterized the binding of c-di-GMP in the GAF domain of PdtaS using biochemical analysis, genetics, molecular docking, FRET microscopy, and structural modelling, and found that a pdtaS knockout in Mycobacterium smegmatis leads to compromised growth on amino acid deficient media and global transcriptional dysregulation. The perturbation of the c-di-GMP-PdtaS-PdtaR axis causes a cascade of cellular changes, as revealed by a multiparametric systems' approach of transcriptomics, unbiased metabolomics, and lipid analyses.
Article
Multidisciplinary Sciences
Maria A. Schumacher, Kelley A. Gallagher, Neil A. Holmes, Govind Chandra, Max Henderson, David T. Kysela, Richard G. Brennan, Mark J. Buttner
Summary: Filamentous actinobacteria of the genus Streptomyces have a complex lifecycle, which is regulated by c-di-GMP. The RsiG protein binds WhiG with different binding motifs. RsiG proteins from different origins show structural and functional differences, indicating a gene duplication event in evolutionary history.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Ainelen Piazza, Lucia Parra, Lucila Ciancio Casalini, Federico Sisti, Julieta Fernandez, Jacob G. Malone, Jorgelina Ottado, Diego O. Serra, Natalia Gottig
Summary: This study revealed the regulatory role of c-di-GMP in the oxidation of Mn(II) by promoting the expression of manganese-oxidizing peroxidase enzymes. It was also found that high levels of c-di-GMP were correlated with increased biofilm formation and enhanced groundwater Mn(II) oxidation capacities. These findings have important implications for biotechnological applications.
Article
Microbiology
Tianyi Chen, Meng Pu, Sundharraman Subramanian, Dan Kearns, Dean Rowe-Magnus
Summary: Elevated c-di-GMP levels inhibit the swimming motility of Vibrio vulnificus and promote biofilm formation, aggregation, and oyster colonization, while attenuating its virulence in mice.
Article
Biotechnology & Applied Microbiology
Gaoge Xu, Lichuan Zhou, Guoliang Qian, Fengquan Liu
Summary: This study identified a diguanylate cyclase that interacts with a phosphodiesterase to specifically regulate antibiotic biosynthesis. The researchers provide direct evidence that the diguanylate cyclase and phosphodiesterase form a complex and indirect evidence to suggest that they may balance a local c-di-GMP pool to control antibiotic production. These findings represent an important discovery regarding the mechanism of a diguanylate cyclase and phosphodiesterase pair in controlling specific c-diGMP signaling pathways.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Xiaobo Liu, Bin Cao, Liang Yang, Ji-Dong Gu
Summary: Biofilm formation and biofilm-induced biodeterioration have significant impacts on the community. Cyclic dimeric guanosine monophosphate (c-di-GMP) is a key signaling molecule in bacteria, regulating various bacterial processes. Understanding the regulatory mechanisms of c-di-GMP would benefit the control of bacterial processes and the development of anti-biofilm technologies.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Microbiology
Amanda L. Miller, Lauren K. Nicastro, Shingo Bessho, Kaitlyn Grando, Aaron P. White, Yi Zhang, Gillian Queisser, Bettina A. Buttaro, Cagla Tuekel
Summary: This study reveals the importance of nitrate in regulating the dispersion of S. Typhimurium from biofilms and its invasion of epithelial cells. Nitrate represses the production of curli and activates flagella through modulation of intracellular c-di-GMP levels. These findings provide insights into the role of nitrate and c-di-GMP signaling in pathogen fitness during infection.
Article
Microbiology
Amanda L. Miller, Lauren K. Nicastro, Shingo Bessho, Kaitlyn Grando, Aaron P. White, Yi Zhang, Gillian Queisser, Bettina A. Buttaro, Cagla Tukel
Summary: Nitrate serves as a key environmental cue during Salmonella infection, inducing dispersal of the pathogen from biofilms by repressing biofilm component production and activating flagella. This regulatory role of nitrate in the switch between sessile and motile lifestyles enhances pathogen fitness during infection.
Article
Microbiology
Jiwon Baek, Hyunjin Yoon
Summary: Cyclic di-GMP is a signaling molecule that controls the transition between motile and nonmotile lifestyles in bacteria. It regulates biofilm formation and dispersal, and it can alter carbon metabolic pathways in Salmonella enterica serovar Typhimurium.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
J. Allombert, C. Jaboulay, C. Michard, C. Andrea, X. Charpentier, A. Vianney, P. Doublet
Summary: The Legionella pneumophila Icm/Dot T4SS can translocate a large number of effectors, and research has found that their delivery is controlled by specific timing to effectively manipulate host cells. The timed delivery of effectors depends not only on chaperone protein interactions but also involves cyclic-di-GMP signaling.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Matthias Christen, Cassandra Kamischke, Hemantha D. Kulasekara, Kathleen C. Olivas, Bridget R. Kulasekara, Beat Christen, Toni Kline, Samuel I. Miller
Article
Multidisciplinary Sciences
Jonathan E. Venetz, Luca Del Medico, Alexander Wolfle, Philipp Schachle, Yves Bucher, Donat Appert, Flavia Tschan, Carlos E. Flores-Tinoco, Marielle van Kooten, Rym Guennoun, Samuel Deutsch, Matthias Christen, Beat Christen
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Biophysics
Nadine Lobsiger, Jonathan E. Venetz, Michele Gregorini, Matthias Christen, Beat Christen, Wendelin J. Stark
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Microbiology
Bidong D. Nguyen, Miguelangel Cuenca, Johannes Hartl, Ersin Guel, Rebekka Bauer, Susanne Meile, Joel Ruethi, Celine Margot, Laura Heeb, Franziska Besser, Pau Perez Escriva, Celine Fetz, Markus Furter, Leanid Laganenka, Philipp Keller, Lea Fuchs, Matthias Christen, Steffen Porwollik, Michael McClelland, Julia A. Vorholt, Uwe Sauer, Shinichi Sunagawa, Beat Christen, Wolf-Dietrich Hardt
CELL HOST & MICROBE
(2020)
Article
Biochemistry & Molecular Biology
Carlos Eduardo Flores-Tinoco, Flavia Tschan, Tobias Fuhrer, Celine Margot, Uwe Sauer, Matthias Christen, Beat Christen
MOLECULAR SYSTEMS BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Ioana Sandu, Dario Cerletti, Manfred Claassen, Annette Oxenius
NATURE COMMUNICATIONS
(2020)
Article
Cell Biology
Ioana Sandu, Dario Cerletti, Nathalie Oetiker, Mariana Borsa, Franziska Wagen, Ilaria Spadafora, Suzanne P. M. Welten, Ugne Stolz, Annette Oxenius, Manfred Claassen
Editorial Material
Microbiology
Beat Christen
Summary: The study demonstrates that under severe stress conditions, Serratia bacteria deactivate their CRISPR immune system to increase the uptake of potentially beneficial plasmids.
NATURE MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Marielle J. F. M. van Kooten, Clio A. Scheidegger, Matthias Christen, Beat Christen
Summary: Rewriting genomes allows for complete annotation of gene regulatory elements. Here the authors compare endogenous and rewritten segments of a genome and find extensive transcriptional changes, based on which they formulate design principles that aid in the programming of biological systems.
NATURE COMMUNICATIONS
(2021)