Review
Biochemistry & Molecular Biology
Zuzanna Samsel, Justyna Sekretarska, Anna Osinka, Dorota Wloga, Ewa Joachimiak
Summary: Motile cilia and homologous organelles, such as flagella, are early evolutionary inventions that play crucial roles in survival and reproduction of primitive eukaryotic cells. Among them, typical motile cilia have complex, two-phase movements and contain central apparatus which is important for ciliary beating regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Kai Cai, Yanhe Zhao, Lei Zhao, Nhan Phan, Yuqing Hou, Xi Cheng, George B. Witman, Daniela Nicastro
Summary: The study identified two proteins, FAP42 and FAP246, localized to the L-shaped C1b projection of the central apparatus (CA) and their interaction with the candidate CA protein FAP413. The complex of FAP246-FAP413-FAP42 is essential for stable assembly of the C1b, C1f, and C2b projections, and their loss leads to ciliary motility defects.
JOURNAL OF CELL SCIENCE
(2021)
Review
Cell Biology
Takuji Ishikawa, Hironori Ueno, Toshihiro Omori, Kenji Kikuchi
Summary: This article explores the roles of cilia and centrosomes in eukaryotic cells and their dynamics, motions, and forces at the molecular, organelle, and tissue levels. The structure and function of cilia and centrosomes are closely related, requiring a comprehensive understanding from the molecular level to the tissue level.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2021)
Article
Biology
Hiroshi Yamaguchi, Motohiro Morikawa, Masahide Kikkawa, Alan Brown
Summary: Outer arm dynein (OAD) is the main force generator of ciliary beating. The docking mechanism of OAD onto the ciliary doublet microtubule (DMT) in vertebrates is not clear. Calaxin/Efcab1 and Armc4 were analyzed for their functions in the OAD-DMT interaction in zebrafish spermatozoa. Calaxin(-/-) OADs were found to be tethered to DMT through DC components other than Calaxin, and recombinant Calaxin could rescue the deficient DC structure and the OAD instability. The roles of Calaxin and Armc4 in the OAD-DMT interaction were demonstrated, suggesting the stabilizing process of OAD docking onto DMT in vertebrates.
Article
Biochemistry & Molecular Biology
Miao Gui, Xiangli Wang, Susan K. Dutcher, Alan Brown, Rui Zhang
Summary: The authors used cryo-EM to build atomic models of the central apparatus of motile cilia from Chlamydomonas reinhardtii and identified 48 different CA-associated proteins. They revealed mechanisms for asymmetric and periodic protein binding to microtubules and discovered a novel class of microtubule inner protein. These structures provide a foundation for further understanding ciliary motility and related diseases.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Deniz Conkar, Elif Nur Firat-Karalar
Summary: The primary cilium is a crucial microtubule-based structure in eukaryotic organisms, functioning as a signaling center with profound effects on organ systems. The microtubule-based axoneme at the core of the primary cilium supports its shape and regulates its structure, length, and stability. Recent technological advances have revealed a more complex picture of the primary cilium, offering new avenues for future research.
Article
Medicine, General & Internal
Rosana Blanco-Manez, Miguel Armengot-Carceller, Teresa Jaijo, Francisco Vera-Sempere
Summary: Diagnosis testing for primary ciliary dyskinesia (PCD) involves multiple investigations, including high-speed video-microscopy, genetic testing, and transmission electron microscopy (TEM). However, some PCD variants cannot be diagnosed through TEM, which was traditionally considered the gold standard. This study identified a previously undescribed axonemal defect that affects peripheral doublets tilting, present in both PCD-confirmed patients and those with normal ultrastructure PCD subtypes. The analysis of axonemal asymmetry could enhance the diagnosis of PCD.
Article
Genetics & Heredity
Francesca Paola Luongo, Alice Luddi, Rosetta Ponchia, Rossella Ferrante, Sara Di Rado, Eugenio Paccagnini, Mariangela Gentile, Pietro Lupetti, Raffaella Guazzo, Alfredo Orrico, Liborio Stuppia, Paola Piomboni
Summary: Primary ciliary dyskinesia (PCD) is a genetic disorder characterized by abnormal ciliary motion. A case study reports a male patient with severe asthenozoospermia but no respiratory symptoms, who carries a homozygote mutation in the CCDC103 gene. The spermatozoa of the patient showed complete loss of inner and outer dynein arms and immotility, while the respiratory cilia still had partial presence of dynein arms. This case enriches the understanding of CCDC103 variants and associated clinical phenotypes in PCD.
FRONTIERS IN GENETICS
(2023)
Article
Medicine, Research & Experimental
Nafisa Nuzhat, Kristof Van Schil, Sandra Liakopoulos, Miriam Bauwens, Alfredo Duenas Rey, Stephan Kaeseberg, Melanie Jaeger, Jason R. Willer, Jennifer Winter, Hanh M. Truong, Nuria Gruartmoner, Mattias Van Heetvelde, Joachim Wolf, Robert Merget, Sabine Grasshoff-Derr, Jo Van Dorpe, Anne Hoorens, Heidi Stoehr, Luke Mansard, Anne-Francoise Roux, Thomas Langmann, Katharina Dannhausen, David Rosenkranz, Karl M. Wissing, Michel Van Lint, Heidi Rossmann, Friederike Haeuser, Peter Nuernberg, Holger Thiele, Ulrich Zechner, Jillian N. Pearring, Elfride De Baere, Hanno J. Bolz
Summary: Mutations in CEP162, a protein involved in centrosome and microtubule function, cause late-onset retinitis pigmentosa by impairing cilia formation and function in the retina. The mutant CEP162 protein is expressed and localized properly in the mitotic spindle but is absent from primary and photoreceptor cilia. Knockdown of Cep162 in developing mouse retina leads to increased cell death, which is rescued by expression of the mutant protein.
JOURNAL OF CLINICAL INVESTIGATION
(2023)
Article
Multidisciplinary Sciences
Hao Liu, Jianqun Zheng, Lei Zhu, Lele Xie, Yawen Chen, Yirong Zhang, Wei Zhang, Yue Yin, Chao Peng, Jun Zhou, Xueliang Zhu, Xiumin Yan
Summary: The formation of axonemal central pair microtubules in mammals requires the cooperative action of Wdr47, Camsaps, and Katanin. These proteins work together to ensure the initiation and proper positioning of central microtubules, with deficiencies leading to ciliary dysfunctions.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Jonathon Howard, Alexander Chasteen, Xiaoyi Ouyang, Veikko F. Geyer, Pablo Sartori
Summary: Cilia and flagella are organelles that propel cells and drive fluid flow. The bending waves generated by dynein motor proteins play a crucial role in this process, but the exact location of these force-generating dyneins is still unclear. Researchers used a molecular-mechanics approach to predict the location of these dyneins and verified their predictions using cryogenic electron microscopy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Genetics & Heredity
Chaofeng Tu, Jiangshan Cong, Qianjun Zhang, Xiaojin He, Rui Zheng, Xiaoxuan Yang, Yang Gao, Huan Wu, Mingrong Lv, Yayun Gu, Shuai Lu, Chunyu Liu, Shixiong Tian, Lanlan Meng, Weili Wang, Chen Tan, Hongchuan Nie, Dongyan Li, Huan Zhang, Fei Gong, Liang Hu, Guangxiu Lu, Wenming Xu, Ge Lin, Feng Zhang, Yunxia Cao, Yue-Qiu Tan
Summary: Research has identified the crucial role of bi-allelic DNAH10 variants in causing asthenoteratozoospermia with MMAF in males, leading to flagellar assembly defects in sperm. This finding provides guidance for genetic counseling related to MMAF and offers important insights into the diagnosis of this condition.
AMERICAN JOURNAL OF HUMAN GENETICS
(2021)
Article
Multidisciplinary Sciences
Ewa Joachimiak, Anna Osinka, Hanan Farahat, Bianka Swiderska, Ewa Sitkiewicz, Martyna Poprzeczko, Hanna Fabczak, Dorota Wloga
Summary: Motile cilia are complex cell organelles capable of active movement, with a highly conserved central apparatus composed of two microtubules and several large microtubule-bound projections, including the C1b/C1f supercomplex. In the ciliate Tetrahymena thermophila, C1b/C1f contains evolutionarily conserved proteins. Deletion of genes encoding certain proteins in C1b/C1f led to loss of the C1b projection and abnormal ciliary motion in the model organism.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Miao Gui, Jacob T. Croft, Davide Zabeo, Vajradhar Acharya, Justin M. Kollman, Thomas Burgoyne, Johanna L. Hoog, Alan Brown
Summary: This study used single-particle cryo-electron microscopy methods to investigate the structure of human ciliary microtubules. Two types of microtubules were studied, and atomic models were built. It was discovered that SPACA9 is a polyspecific microtubule inner protein capable of binding different types of microtubules.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Editorial Material
Neurosciences
Madison T. Gray, Julie L. Lefebvre
Summary: Visual impairments in albinism are caused by reduced ipsilateral retinal projections. In this issue of Neuron, Slavi, Balasubramanian, and colleagues1 demonstrate how low CyclinD2 in the ciliary marginal zone disrupts the generation of ipsilaterally projecting retinal ganglion cells (RGCs) and show that restoring CyclinD2 improves vision in albino mice.
Article
Biology
Lei Zhao, Yuqing Flou, Nathan A. McNeill, George B. Witman
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2020)
Article
Multidisciplinary Sciences
Tyler Picariello, Yuqing Hou, Tomohiro Kubo, Nathan A. McNeill, Haruaki Yanagisawa, Toshiyuki Oda, George B. Witman
Article
Cell Biology
Yuqing Hou, Lei Zhao, Tomohiro Kubo, Xi Cheng, Nathan McNeill, Toshiyuki Oda, George B. Witman
Summary: This study identified FAP70, FAP65, and FAP147 as the first defining components of the C2a projection in cilia, highlighting their crucial role in ciliary function.
JOURNAL OF CELL SCIENCE
(2021)
Article
Cell Biology
Kai Cai, Yanhe Zhao, Lei Zhao, Nhan Phan, Yuqing Hou, Xi Cheng, George B. Witman, Daniela Nicastro
Summary: The study identified two proteins, FAP42 and FAP246, localized to the L-shaped C1b projection of the central apparatus (CA) and their interaction with the candidate CA protein FAP413. The complex of FAP246-FAP413-FAP42 is essential for stable assembly of the C1b, C1f, and C2b projections, and their loss leads to ciliary motility defects.
JOURNAL OF CELL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Renhai Peng, Yanchao Xu, Shilin Tian, Turgay Unver, Zhen Liu, Zhongli Zhou, Xiaoyan Cai, Kunbo Wang, Yangyang Wei, Yuling Liu, Heng Wang, Guanjing Hu, Zhongren Zhang, Corrinne E. Grover, Yuqing Hou, Yuhong Wang, Pengtao Li, Tao Wang, Quanwei Lu, Yuanyuan Wang, Justin L. Conover, Hassan Ghazal, Qinglian Wang, Baohong Zhang, Marc Van Montagu, Yves Van de Peer, Jonathan F. Wendel, Fang Liu
Summary: This study obtained and assembled chromosome-scale genome sequences for two wild tetraploid cotton species (Ge and Gs) and one early domesticated species (Ghp). By conducting phylogenomic analysis, the evolutionary relationships and genomic structural variations of these cotton species were resolved. The study also identified genes associated with fiber length and environmental adaptation. These findings provide valuable insights into cotton evolution and agricultural applications.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Engineering, Electrical & Electronic
Kaixuan Wang, Sheng Tang, Jing Ke, Yuqing Hou
Summary: This article presents the design of a broadband compact small active magnetic antenna for receiving Loran-C signals both indoors and outdoors. The antenna consists of a magnetic antenna part and an active circuit part. The magnetic antenna part utilizes a new H-shaped magnetic antenna with a high quality factor, achieved through material selection and antenna shape simulation. The active circuit part includes an amplifier and a filter designed using an operational amplifier, as well as a single-tube double tuning circuit for frequency selection to improve the signal-to-noise ratio (SNR). Through these designs, effective reception of Loran-C signals is achieved in both indoor and outdoor environments. The processed and tested Loran-C active magnetic antenna successfully receives a clear pulse group signal at a frequency of 99.21 kHz. The antenna length is 2.75 cm, and the circuit board size is 10 x 3 cm. The SNR is measured to be 12.04 dB outdoors and 16.48 dB indoors.
IEEE SENSORS JOURNAL
(2023)
Article
Multidisciplinary Sciences
Yuqing Hou, Xi Cheng, George B. Witman
Summary: Chlamydomonas reinhardtii is an important model organism for cellular process studies, and protein tagging is an increasingly important tool. To overcome the limitations of conventional methods, we developed a strategy to tag endogenous genes in Chlamydomonas reinhardtii. We successfully tagged two genes and confirmed the effectiveness of the tagging.
Article
Multidisciplinary Sciences
Peng Huang, Jing-Jing Wu, Jin-Wei Zhang, Yu-Qing Hou, Ping Zhu, Rong Yin, Rong-Bin Yu, Yun Zhang, Ming Yue, Wei Hou
Summary: This study investigates the connection between retinoic acid-inducible gene I-like receptor gene polymorphism and hepatitis C chronicity in the Chinese Han population. It found that DHX58 rs2074158-G allele and IFIH1 rs10930046-C allele are associated with chronic hepatitis C (CHC). The risk of CHC increases with the number of unfavorable genotypes carried by the infected person. IFIH1 rs10930046, DHX58 rs2074158, age, ALT, and AST levels are independent predictors of CHC.
Review
Genetics & Heredity
Muhammad Jawad Umer, Jie Zheng, Mengying Yang, Raufa Batool, Aamir Ali Abro, Yuqing Hou, Yanchao Xu, Haileslassie Gebremeskel, Yuhong Wang, ZhongLi Zhou, Xiaoyan Cai, Fang Liu, Baohong Zhang
Summary: The soil-borne pathogen Verticillium dahliae, also known as The Cotton Cancer, causes Verticillium wilt in cotton crops, a destructive disease with a global impact. The pathogen employs virulence mechanisms such as releasing enzymes that degrade cell walls, activating virulence-related genes, and using protein effectors to infect cotton plants. On the other hand, cotton plants have developed defense mechanisms to combat the impact of V. dahliae.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Biochemical Research Methods
Jintao Li, Lizhi Zhang, Jia Liu, Diya Zhang, Dizhen Kang, Beilei Wang, Xiaowei He, Heng Zhang, Yizhe Zhao, Hongbo Guo, Yuqing Hou
Summary: This paper proposes a universally applicable adaptive parameter selection method based on maximizing the probability of data (MPD) strategy in fluorescent molecular tomography (FMT) regularization methods. The strategy establishes a regularization parameter model using maximum a posteriori (MAP) estimation and maximum likelihood (ML) estimation and determines the stable optimal parameters through multiple iterative estimates. Numerical simulations and in vivo experiments demonstrate that the MPD strategy can provide stable regularization parameters for both L-2 or L-1 norm-based regularization algorithms and achieve good reconstruction performance.
JOURNAL OF BIOPHOTONICS
(2023)
Article
Engineering, Marine
Yuqing Hou, Hui Li, Hong Chen, Shengnan Shen, Fei Duan, Wei Wei, Jiayue Wang, Yicang Huang, Xiawei Guan, Yinghao Liao
Summary: This study proposes a novel SEConv1D framework for predicting the hydrodynamic characteristics of unidentified underwater vehicles. The framework combines Conv1D and SENet, and uses a hydrodynamic dataset based on CFD that is verified by experiments. The results show that the framework can accurately predict the total resistance coefficient (Cd) of REMUS UUV and SUBOFF AFF-1, with a small error compared to experimental data and significant reductions in error compared to other popular network models.
Article
Agronomy
Joseph Wanjala Kilwake, Muhammad Jawad Umer, Yangyang Wei, Teame Gereziher Mehari, Richard Odongo Magwanga, Yanchao Xu, Yuqing Hou, Yuhong Wang, Margaret Linyerera Shiraku, Joy Nyangasi Kirungu, Xiaoyan Cai, Zhongli Zhou, Renhai Peng, Fang Liu
Summary: Drought and salinity are the most devastating abiotic factors worldwide, causing severe problems in agriculture. Plants have developed complex molecular mechanisms, including S-adenosyl-L-methionine synthetase (SAMS) genes, to adapt to these harsh conditions. In this study, we identified and characterized 16 SAMS genes in upland cotton and found that GhSAMS2 was highly induced under drought and salt stress. Knockdown of GhSAMS2 expression through virus-induced gene silencing (VGIS) led to increased susceptibility of transgenic plants to drought and salt stress. Our findings provide insights into the structure, classification, and role of SAMS genes in abiotic stress response in upland cotton.
Article
Food Science & Technology
Zihan Song, Yanli Zhang, Yongrui Ti, Huitian Qiao, Chen Niu, Yuqian Ban, Xiaoxiao Wang, Yuqing Hou, Ruiwen Lu
Summary: To prevent drug-resistant fungi, cinnamic acid was combined with poly-dopamine magnetic nanoparticles to create an effective antifungal material. The resulting CA-DMPs showed great antifungal activity and could be reused multiple times. The composite particles damaged fungal cells and affected energy metabolism and genetic expression. In vitro antifungal experiments on cherry tomatoes confirmed the material's application potential in food preservation.
Article
Plant Sciences
Xiaoyan Cai, Yuqing Hou, Umer Jawad Muhammad, Heng Wang, Yanchao Xu, Jie Zheng, Yuhong Wang, Fang Liu, Zhongli Zhou, Jinping Hua, Kunbo Wang
Summary: This study conducted a comprehensive analysis of the pollen morphology of 33 cotton species and varieties. The results showed that these samples had common morphological features, but also exhibited differences in size, spine shape, spine density, and length. In addition, the cotton species and varieties were grouped into three clusters based on genetic distance. These findings provide important information for future systematic and phylogenetic studies of the Gossypium genus.
JOURNAL OF COTTON RESEARCH
(2023)
Article
Medicine, Research & Experimental
Yintang Wang, Yu Geng, Ou Zhang, Qin Xu, Yajun Xue, Boda Zhou, Ping Zhang, Aihua Li, Bao Li, Biao Xu, Guangshu Han, Bin Li, Bin Liu, Bin Wang, Bing Fu, Bo Yu, Bosong Yang, Caidong Luo, Changqian Wang, Changyong Liu, Chuanliang Liang, Chuanyu Gao, Chunlin Lai, Chuntong Wang, Chunyan Zhang, Chunyang Wu, Congliang Zhang, Cui Bin, Lan Huang, Daoquan Peng, Dawen Xu, Di Wu, Dongmei Zhu, Dongsheng Chai, Dongyan Li, Fakuan Tang, Jun Xiao, Fang Zhao, Fangfang Huang, Fanju Meng, Fengwei Li, Fudong Gan, Gang Xu, Gengsheng Sang, Genshan Ma, Guixia Zhang, Guizhou Tao, Guo Li, Guoduo Chen, Guoqin Xin, Guoqing Li, Guosheng Fu, Guoxiong Chen, Hailong Lin, Haiping Guo, Haiyun Lin, Hong Jiang, Hong Liu, Hong Luan, Hong Zhang, Honghua Deng, Hongwei Li, Honhju Wang, Hualing Liu, Hui Dong, Hui Liu, Huifang Zhang, Huifeng Wang, Huimin Chu, Jiabin Xi, Jian Yang, Jianfeng Ye, Jianhao Li, Jianhong Tao, Jianwen Liu, JiaoMei Yang, Jiawang Ding, Jiayi Tong, Jie Chen, Jie Jiang, Jie Yang, Jifu Li, Jinchuan Yan, Jing Hu, Jing Xu, Jingfeng Wang, Jinglan Diao, Jingshan Zhao, Jinru Wei, Jinxing Yi, Jinzi Su, Jiong Tang, Jiyan Chen, Jiyan Yin, Juexin Fan, Jun Guan, Junbo Ge, Junming Liu, Junping Deng, Junping Fang, Junxia Li, Kaihong Chen, Kalan Luo, Keng Wu, Lang Ji, Lang Li, Li Jiang, Li Wei, Lijun Meng, Likun Ma, Lilong Tang, Lin Wang, Lin Wei, Ling Li, Ling Tao, Liqiong Yang, Lirong Wu, Man Zhang, Kaiming Chen, Meisheng Lai, Miao Tian, Mingcheng Bai, Minghua Han, Moshui Chen, Naiyi Liang, Nan Jia, Peiying Zhang, Peng Qu, Pengfei Zhang, Ping Chen, Ping Hou, Ping Xie, Pingshuan Dong, Qiang Wu, Qiang Xie, Qiaoqing Zhong, Qichun Wang, Qinfeng Su, Rong Chang, Rong Lin, Ruiping Zhao, Shaobin Jia, Shaoping Nie, Shaowu Ye, Shenghu He, Shengyong Chen, Shixin Ma, Shuangbin Li, Shuanli Xin, Shudong Xia, Shuhua Zhang, Shuqiu Qu, Shuren Ma, Siding Wang, Songbai Li, Suxin Luo, Tao Liu, Tao Zhang, Tian Tuo, Tianchang Li, Tianlun Yang, Tianmin Du, Tongguo Wu, Wei Liu, Wei Mao, Wei Tuo, Wei Wang, Weihong Jiang, Weijian Huang, Weijun Liu, Weiqing Fan, Weiting Xu, Wenhua Lin, Xi Su, Xia Chen, Xianan Zhang, Xianghua Fu, Xiangjun Yang, Xianxian Zhao, Xiaochuan Ma, Xiaofei Sun, Xiaojun Wang, Xiaolan Li, Xiaolei Li, Xiaoli Yang, Xiaoping Chen, Xiaoqin Zhang, Xiaoshu Cheng, Xiaowei Peng, Xiaowen Ma, Xiaoyong Qi, Xiaoyun Feng, Ximing Chen, Xin Tang, Xingsheng Tang, Xingsheng Zhao, Xiufeng Chen, Xudong Li, Xue Li, Xuebo Liu, Xuemei Peng, Yaling Han, Yan Wang, Yanbo Niu, Yang Yu, Yang Zheng, Yanli Wang, Yanlie Zheng, Yansong Guo, Yanzong Yang, Yi Huang, Yin Liu, Ying Guo, Yingchao Luo, Yinglu Hao, Yingxian Sun, Yingzhong Lin, Yitong Ma, Yong Guo, Yong Li, Yongdong Li, Yonglin Zhang, Yuanzhe Jin, Yue Li, Yuehua Huang, Yuemin Sun, Yuheng Yang, Yuhua Zhu, Yuhuan Shi, Yulan Zhao, Yuqing Hou, Zeqi Zheng, Zesheng Xu, Zewei Ouyang, Zeyuan He, Zhan Lv, Zhanquan Li, Zhaofa He, Zheng Ji, Zheng Zhang, Zhenguo Ji, Zhenqi Su, Zhenyu Yang, Zhihong Ou, Zhijian Yang, Zhiming Yang, Zhirong Wang, Zhiyuan Song, Zhongshan Wang, Zuyi Yuan
Summary: In patients with NSTEACS who were already taking P2Y12 receptor inhibitors, reloading of these inhibitors was associated with a decreased risk of in-hospital major adverse cardiac events (MACE) and all-cause death, without an increased risk of major bleeding.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2023)
