Article
Food Science & Technology
Dj'essica Tatiane Raspe, Camila da Silva, Silvio Claudio da Costa
Summary: This review analyzes and discusses emerging techniques for extracting natural sweeteners and active compounds from Stevia rebaudiana Bertoni, demonstrating their impacts on extraction and comparing them with conventional techniques. These new technologies offer the advantages of simplicity, reduced energy and solvent usage, making them potentially suitable for industrial applications, but they still face limitations.
Article
Agricultural Engineering
Marcos Vinicius Silva de Andrade, Renato Delmondez de Castro, Diego da Silva Cunha, Valdir Gomes Neto, Maria Gabriela Aparecida Carosio, Antonio Gilberto Ferreira, Lourdes Cardoso de Souza-Neta, Luzimar Gonzaga Fernandez, Paulo Roberto Ribeiro
Summary: Research demonstrates that Stevia rebaudiana's response to light conditions affects the accumulation of antioxidant-like metabolites in its leaves, possibly linked to total phenolic content. Variations in SOD activity among plants under different photoperiods suggest a synergistic interaction between SOD isoforms and phenolic compounds to mitigate oxidative stress caused by light exposure.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Agronomy
Asish Kumar Ghose, Siti Nor Akmar Abdullah, Muhammad Asyraf Md Hatta, Puteri Edaroyati Megat Wahab
Summary: A plant tissue culture protocol from stevia was optimized for the production of planting materials and rebaudioside A, a natural sweetener. By adjusting the culture medium and supplement concentrations, the survival rate and callus production were improved, resulting in higher yields of shoots and roots. The highest concentration of rebaudioside A was obtained using specific media conditions and correlated with the expression level of a biosynthetic gene.
Article
Agriculture, Multidisciplinary
Sun Yu-ming, Huang Xiao-lei, Zhang Ting, Yang Yong-heng, Cheng Xiao-fang, Xu Xiao-yang, Yuan Hai-yan
Summary: The study found that potassium deficiency significantly reduces steviol glycosides content in stevia leaves, inhibits the expression of genes related to steviol glycosides synthesis, and affects the activity of sugar metabolism-related enzymes. There is a significant positive correlation between sucrose, glucose, and steviol glycosides content.
JOURNAL OF INTEGRATIVE AGRICULTURE
(2021)
Article
Horticulture
Monika Tuleja, Michal Santocki, Michal Dziurka, Krystyna Musial, Ewa Capecka, Marta Libik-Konieczny
Summary: Seed germination in Stevia rebaudiana is poor, limiting generative reproduction. The study suggests that improper levels of gibberellins and redirection of metabolic pathways for secondary metabolites biosynthesis may cause difficulties in pollen tube germination, affecting seed development in the plant.
SCIENTIA HORTICULTURAE
(2021)
Article
Agricultural Engineering
Fatemeh Afshari, Fatemeh Nakhaei, Seyedgholamreza Mosavi, Mohammadjavad Seghatoleslami
Summary: The poor germination capacity of stevia is a major problem in cultivation, and water restrictions stress negatively affects its germination, growth, yield, and secondary metabolites. Nutri-priming and foliar nutrition treatments with PBZ, NKF, and SA improved morpho-physiological traits of stevia under water restriction stress. The integrated application of NKF, PBZ, and SA can promote germination performance, protect from negative effects of WRS, and enhance stress tolerance, growth, yield, and secondary metabolite synthesis in stevia.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Chemistry, Multidisciplinary
Teresa Leszczynska, Barbara Pieklo, Aneta Kopec, Benno F. Zimmermann
Summary: This study compares the basic nutrients, steviol glycosides, antioxidants, and antioxidant activity in dried leaves of Stevia rebaudiana Bertoni from Poland, Paraguay, and Brazil. The findings show that leaves from Poland have higher dietary fiber content, while those from Brazil and Paraguay have lower steviol glycoside contents. Furthermore, dried leaves from Poland have higher vitamin C content but lower antioxidant activity compared to the other two countries.
APPLIED SCIENCES-BASEL
(2021)
Article
Plant Sciences
Auguste Judickaite, Veronika Lyushkevich, Irina Filatova, Vida Mildaziene, Rasa Zukiene
Summary: This study aimed to determine the effect of pre-sowing seed treatment with cold plasma and electromagnetic field on the amount and ratio of steviol glycosides in the leaves of Stevia rabaudiana Bertoni. The results showed that these treatments could increase the accumulation of steviol glycosides but may have a negative impact on the content of other secondary metabolites.
Article
Agricultural Engineering
Zoe Le Bihan, Cecile Hastoy, Patrick Cosson, Philippe Boutie, Dominique Rolin, Valerie Schurdi-Levraud
Summary: The study on cultivating Stevia rebaudiana in temperate conditions found that avoiding harvest in the first year can facilitate better crop establishment, overwintering, and overall yield. Variability in response to overwintering, SG yield, and dry leaf biomass among different genotypes was observed, with a decrease in these traits for plants harvested in the first year.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Medicine, Research & Experimental
Jakub Michal Kurek, Joanna Mikolajczyk-Stecyna, Zbigniew Krejpcio
Summary: This study reveals the potential of steviol glycosides in regulating lipid metabolism and shows their impact on specific gene expression. However, further research, including human trials, is needed to confirm these effects.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Morteza Sheikhalipour, Behrooz Esmaielpour, Gholamreza Gohari, Maryam Haghighi, Hessam Jafari, Habib Farhadi, Muhittin Kulak, Andrzej Kalisz
Summary: The study investigated the effects of TiO2 NPs and Cs-Se NPs on the growth and physiological characteristics of stevia under salt stress.
Article
Biochemistry & Molecular Biology
Roberta Ascrizzi, Marinella De Leo, Laura Pistelli, Claudia Giuliani, Ylenia Pieracci, Barbara Ruffoni, Carlo Mascarello, Gelsomina Fico, Guido Flamini, Luisa Pistelli
Summary: The Nemo's Garden (R) project is an interesting intervention to address the climate crisis in areas with scarce cultivable land but abundant water resources. This study found differences in the micromorphological, biochemical, and phytochemical characteristics between Stevia rebaudiana grown underwater and on land.
Article
Plant Sciences
Vali Ollah Ghasemi-Omran, Abazar Ghorbani, Seyyed Abdolkarim Sajjadi-Otaghsara
Summary: Melatonin at 5 and 10 μM can improve growth parameters, photosynthetic pigments, proline, and soluble sugar contents, reduce electrolyte leakage, and decrease malondialdehyde and ROS accumulation in stevia under 25 mM NaCl toxicity. Additionally, melatonin can maintain ionic balance by increasing the accumulation of K, N, Ca, and P and decreasing the accumulation of Cl and Na. Moreover, melatonin can enhance the accumulation of rebaudioside A and stevioside by upregulating the expression level of KAH and UGTs genes, indicating its protective role against NaCl toxicity in stevia under appropriate concentrations.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2021)
Article
Biochemical Research Methods
Soo Yee Lee, Khozirah Shaari
Summary: This study investigated the metabolite changes along different developmental stages of a local stevia cultivar, revealing differences in metabolite profiles at different stages, with higher levels of phenolic acids in early stages and higher levels of flavonoids and steviol glycosides in later stages. The findings provide valuable insights for decision making in optimizing harvesting times for higher yields or desired metabolite profiles.
PHYTOCHEMICAL ANALYSIS
(2022)
Article
Plant Sciences
Morteza Sheikhalipour, Gholamreza Gohari, Behrooz Esmaielpour, Sima Panahirad, Maryam Haghmadad Milani, Muhittin Kulak, Tibor Janda
Summary: Drought stress is a major constraint on crop growth and productivity. This study investigated the effects of melatonin and nano-titanium dioxide nanoparticles (TiO2 NPs) on the agronomic, physiological, and biochemical traits of stevia under moderate and severe drought stress. The results showed that the application of melatonin and TiO2 NPs improved plant traits, increased antioxidant activities, proline content, and secondary metabolites, and decreased malondialdehyde levels. The best treatment for alleviating the negative effects of drought stress and increasing secondary metabolites in stevia was foliar application of 100 μM melatonin and 10 mg/L TiO2 NPs.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Article
Biochemistry & Molecular Biology
Katarzyna Sulkowska-Ziaja, Gokhan Zengin, Agnieszka Gunia-Krzyzak, Justyna Popiol, Agnieszka Szewczyk, Magdalena Jaszek, Jerzy Rogalski, Bozena Muszynska
Summary: This study assessed the biosynthetic potential of mycelial cultures of six Ganoderma species and found various bioactive compounds in the extracts. These cultures may be promising candidates for the development of new dietary supplements or pharmaceutical preparations.
Article
Biochemistry & Molecular Biology
Agnieszka Szewczyk, Andreana Marino, Jessica Molinari, Halina Ekiert, Natalizia Miceli
Summary: In this study, in vitro cultures of three species of the genus Ruta were examined. The growth dynamics and secondary metabolite accumulation were analyzed, and the antioxidant capacity and antimicrobial potential were evaluated. The results showed variations in antioxidant capacity and antimicrobial activity among the tested species.
Article
Multidisciplinary Sciences
Agata Ptak, Emilia Moranska, Marzena Warcho, Artur Gurgul, Edyta Skrzypek, Michal Dziurka, Dominique Laurain-Mattar, Rosella Spina, Anita Jaglarz, Magdalena Simlat
Summary: A novel strain of endophytic bacterium Paenibacillus lautus was isolated from Leucojum aestivum plants and found to have the ability to produce alkaloids and growth regulators. Elicitation with the bacterium increased alkaloid production and had various effects on plant growth and metabolism.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Barbara Borczak, Marek Sikora, Joanna Kapusta-Duch, Maria Folta, Agnieszka Szewczyk, Gabriela Ziec, Ivo Doskocil, Teresa Leszczynska
Summary: This study evaluated the impact of using widely grown fruits as functional ingredients in wheat-flour cookies on their antioxidative properties and reduction of acrylamide content. The results showed that fruit-enriched cookies had higher antioxidative properties and significantly reduced acrylamide content compared to control cookies. Wild rose and rowan cookies were the most highly rated and widely accepted by the panelists. This suggests that cookies enriched with wild-grown fruits could be a promising novel snack food.
Article
Biochemistry & Molecular Biology
Karol Jedrejko, Katarzyna Kala, Katarzyna Sulkowska-Ziaja, Agata Krakowska, Piotr Zieba, Krystian Marzec, Agnieszka Szewczyk, Agnieszka Sekara, Jolanta Pytko-Polonczyk, Bozena Muszynska
Summary: This study aimed to determine the content of biologically active substances, total phenolic content, and antioxidant activity of Cordyceps militaris fruiting bodies, mycelia, and food supplements. The results showed that substrate composition and cultivation method influenced the properties of the mushroom materials. An important aspect of the study was the evaluation of bioactive substance content after extraction in a simulated digestive system, which allowed for determining potential bioavailability. The mycelium and fruiting bodies of C. militaris were found to be ideal food supplements and pharmaceutical agents that could provide prohealth substances for humans.
Article
Biochemistry & Molecular Biology
Magdalena Simlat, Agata Ptak, Anita Jaglarz, Agnieszka Szewczyk, Michal Dziurka, Artur Gurgul
Summary: In this study, a new strain of Pantoea vagans, SRS89, was isolated from surface-sterilized stevia seeds and evaluated using various methods. The strain exhibited certain properties, such as yellow color and Gram-negative classification. Genome sequencing and annotation revealed numerous genes involved in transcription, amino acid and carbohydrate metabolism, as well as carotenoid and terpenoid biosynthesis. The strain also showed the ability to synthesize rebaudioside A, a valuable metabolite.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Katarzyna Kala, Wojciech Pajak, Katarzyna Sulkowska-Ziaja, Agata Krakowska, Jan Lazur, Maciej Fidurski, Krystian Marzec, Piotr Zieba, Agata Fijalkowska, Agnieszka Szewczyk, Bozena Muszynska
Summary: This study evaluated the chemical composition of Hypsizygus marmoreus fruiting bodies, mycelium, and in vitro cultures, and found that H. marmoreus mycelium is a good source of indole compounds, bioelements, glucans, and lovastatin. In vitro cultures enriched with zinc and magnesium salts showed increased levels of certain compounds, including 5-methyltryptamine, melatonin, protocatechuic acid, sterols, and total glucans.
Article
Plant Sciences
Katarzyna Sulkowska-Ziaja, Agnieszka Galanty, Agnieszka Szewczyk, Pawel Pasko, Katarzyna Kala, Anna Apola, Irma Podolak, Bozena Muszynska
Summary: This study aimed to evaluate the effects of methyl jasmonate (MeJA) on the production of triterpenes in submerged cultures of Ganoderma applanatum. The results showed that MeJA promoted the production of ganoderic acid A and ganoderic acid C and exhibited significant cytotoxic activity against prostate cancer cells.
Article
Biochemistry & Molecular Biology
Agnieszka Szewczyk, Andreana Marino, Maria Fernanda Taviano, Lucia Cambria, Federica Davi, Monika Trepa, Mariusz Grabowski, Natalizia Miceli
Summary: This study evaluated the effects of cultivation time and different concentrations of plant growth regulators on the biomass and secondary metabolite accumulation of R. montana. The major secondary metabolites in R. montana cultures were coumarins, with xanthotoxin and bergapten as the dominant compounds. The extract obtained from the biomass grown on the 0.1/0.1 LS medium showed the best antioxidant activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Katarzyna Sulkowska-Ziaja, Justyna Robak, Andrzej Szczepkowski, Agnieszka Gunia-Krzyzak, Justyna Popiol, Joanna Piotrowska, Bartlomiej Rospond, Agnieszka Szewczyk, Katarzyna Kala, Bozena Muszynska
Summary: This study analyzed the bioelements and selected metabolites produced in mycelial cultures obtained from different host species. The results showed that mycelial cultures can be a potential source of bioactive compounds and promising naturally derived cytotoxic agents.
Article
Agronomy
Piotr Palka, Bozena Muszynska, Agnieszka Szewczyk, Bozena Pawlowska
Summary: In this study, the phenolic compound content and composition of Lilium candidum adventitious bulbs formed in vitro were determined. The use of zinc oxide nanoparticles (ZnO NPs) in the culture medium, along with LED light spectra dominated by red and blue light, significantly increased the content of phenolic compounds.
Article
Plant Sciences
Magdalena Simlat, Agata Ptak, Tomasz Wojtowicz, Agnieszka Szewczyk
Summary: This study investigated the content of phenolic acids and flavonoids in stevia leaves as affected by treatment with melatonin and germination in NaCl conditions. The results showed that phenolic acids were more abundant than flavonoids in stevia leaves. Treatment with melatonin increased the content of both phenolic acids and flavonoids, and 500μM melatonin under salinity conditions had the most favorable effect on the synthesis of phenolic acids. The content of phenolic compounds also varied depending on the age of the leaves.
Article
Biology
Agnieszka Szewczyk, Mariusz Grabowski, Dominika Zych
Summary: Due to decreasing crop acreages and climate change, there has been increasing interest in alternative sources of bioactive compounds. Plant biotechnology offers the potential for cultivating valuable medicinal plant species using in vitro methods, which are not dependent on climate and soil conditions. This study investigated the ability of Ruta chalepensis bioreactor cultures to produce important linear furanocoumarins and furoquinoline alkaloids.
