Article
Agriculture, Multidisciplinary
Pei Shan, Chi-Tang Ho, Lei Zhang, Xianli Gao, Hong Lin, Ting Xu, Bo Wang, Jiangyan Fu, Ronghai He, Yaqiong Zhang
Summary: This study isolated and identified an APE from Aspergillus niger that can degrade approximately 50% of the B3 subunit and increase proline content in soy sauce. APE is an acidic salt-tolerant serine protease that is optimally active at 40 degrees C and pH 4.0. High salinity affects the spatial structures and catalytic activity of APE, as well as the rigidity of the B3 subunit.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Applied
Yan Ma, Tie-jun Ling, Xiao-qin Su, Bin Jiang, Bo Nian, Li-jiao Chen, Ming-li Liu, Zheng-yan Zhang, Dao-ping Wang, Yong-ying Mu, Wen-wen Jiao, Qian-ting Liu, Ying-hong Pan, Ming Zhao
Summary: The study revealed the important functions of Aspergillus in post-fermented Pu-erh tea, showing its ability to alter the concentrations and compositions of metabolites in tea leaves, mainly involving degradation and production of various compounds.
Article
Microbiology
Thanaporn Laothanachareon, Enrique Asin-Garcia, Rita J. M. Volkers, Juan Antonio Tamayo-Ramos, Vitor A. P. Martins dos Santos, Peter J. Schaap
Summary: Aspergillus niger is a powerful microbial cell factory for producing organic acids. The regulation of the glucose oxidase (Gox) expression system, which is involved in gluconic acid biosynthesis, has been recently discovered. The study found that hydrogen peroxide plays a crucial role as a signaling molecule in inducing this system. The facilitated diffusion of hydrogen peroxide via aquaporin water channels (AQPs) was studied in this research.
Article
Microbiology
Xuejie Li, Lianggang Huang, Lijie Pan, Bin Wang, Li Pan
Summary: The CRISPR/dCas9-mediated histone epigenetic modification system efficiently reprogrammed the expression of target genes in A. niger, leading to increased production of secondary metabolites. Targeted epigenome editing is a promising technology for regulating gene expression in filamentous fungi.
MICROBIOLOGICAL RESEARCH
(2021)
Article
Plant Sciences
Yina Xiao, Fubo Han, Myeong Ji Kim, Kwang Youl Lee, Ik-Soo Lee
Summary: Major bioactive constituents BCA (1) and BCB (2) from Broussonetia papyrifera were synthesized synthetically and subjected to microbial transformation, yielding 10 previously undescribed chalcones. Among them, metabolite 1e showed the highest cytotoxicity in human cancer cell lines.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Environmental Sciences
Tan Phat Chau, Laura Bulgariu, Mythili Saravanan, R. Rajkumar, Arunachalam Chinnathambi, Saleh H. Salmen, G. K. Jhanani
Summary: This research investigates the potential of Aspergillus niger and Aspergillus tubigenesis in bioremediation of tannery effluent. The fungal biomass immobilized on corncob and coir material demonstrated significant multi-pollutant adsorption potential. The study also showed reduced toxicity and improved seed germination of Oriza sativa seeds. Further field investigation is needed to assess the feasibility of this strategy.
ENVIRONMENTAL RESEARCH
(2023)
Article
Food Science & Technology
Arzu Cagri Mehmetoglu, Elif Sezer, Sumeyye Erol
Summary: The study confirmed that silver nanoparticles synthesised by Aspergillus niger have significant antimicrobial and mechanical properties enhancing effects on whey protein concentrate (WPC) based films, while also improving the tensile strength and water barrier properties of the films.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Mengyuan Wei, Pengcheng Chen, Pu Zheng, Xiumei Tao, Xiaowei Yu, Dan Wu
Summary: In this study, a high expression level of aspartic protease from Aspergillus niger was successfully achieved in P. pastoris. Additionally, the highest protein hydrolysis rate for soybean isolate protein degradation was also achieved. The acid protease in this study provides a new protease suitable for the feed industry, which will greatly improve feed utilization and promote the development of the breeding industry.
MICROBIAL CELL FACTORIES
(2023)
Article
Biochemistry & Molecular Biology
Mark Arentshorst, Prajeesh Kooloth Valappil, Laszlo Mozsik, Tonny J. G. Regensburg-Tuink, Sjoerd J. Seekles, Gwen Tjallinks, Marco W. Fraaije, Jaap Visser, Arthur F. J. Ram
Summary: To improve protein production in Aspergillus niger, researchers used CRISPR/Cas9-mediated genome editing to construct strains with up to 10 glucoamylase landing sites (GLSs) in predetermined locations. Each GLS contains the promoter and terminator region of the highly expressed glucoamylase gene (glaA). By introducing unique DNA sequences and designing Cas9-compatible single guide RNAs, targeted integration of a gene of interest can be achieved at specific GLSs.
Review
Plant Sciences
Maria Camila Rodriguez Gallo, R. Glen Uhrig
Summary: This mini-review summarizes the current knowledge of plant-specific protein kinase families involved in alternative splicing (AS) regulation and compares them to their counterparts in animals. The extensive phosphorylation of splicing-related proteins in plants is highlighted, underscoring the importance of investigating the impact of AS on protein and post-translational modifications.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Food Science & Technology
Wenkang Hu, Zeping Liu, Bin Fu, Xiaolong Zhang, Yonggang Qi, Yong Hu, Chao Wang, Dongsheng Li, Ning Xu
Summary: The study found that Aspergillus oryzae was the dominant strain in koji mixed fermentation, with both fungal species growing rapidly during the initial 24 hours of fermentation and then stabilizing. Inoculation of Aspergillus niger led to changes in the contents of various metabolites in koji.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2022)
Review
Developmental Biology
Nikolai Slavov
Summary: Robust single-cell mass spectrometry methods allow accurate quantification of proteins and their modification, providing insights into post-transcriptional regulatory mechanisms and their role in developmental cell fate specification. These methods also enable the analysis of protein conformations and activities, linking protein functions to developmental processes in single cells.
Review
Chemistry, Multidisciplinary
Ronglu Yu, Jia Liu, Yi Wang, Hong Wang, Huawei Zhang
Summary: Aspergillus niger, a common and important fungal species, produces various biomolecules with wide applications as secondary metabolites. This review comprehensively summarizes the biological and chemical aspects of A. niger strains, including their sources, BGCs, secondary metabolites, biological properties, and biosynthetic pathways. Future perspectives on the discovery of more functional biomolecules derived from A. niger are also provided.
FRONTIERS IN CHEMISTRY
(2021)
Article
Agronomy
Ruizhe Zhang, Zefen Yu, Hexin Cui, Chuwen Ye, Min Qiao
Summary: Aspergillus niger and its metabolites exhibit significant nematocidal activity and promote plant growth.
PEST MANAGEMENT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Weiling Lin, Xiaohong Xu, Ruirui Lv, Wei Huang, Hafeez ul Haq, Yuanyuan Gao, Hongli Ren, Canhua Lan, Baoyu Tian
Summary: The improved pectinase activity in Aspergillus niger mutant EIMU2 was likely due to the up-regulated expression of rhamnogalacturonan lyase or pectinesterase, which optimized the synergy amongst different components of pectin-degrading enzymes. The proteomic analysis revealed a multiple enzyme system in mutant EIMU2 for pectin degradation, with higher quantities of certain proteins compared to the wild-type strain. The sequences of key pectin-degrading enzyme genes were found to be consistent between A. niger EIM-6 and mutant EIMU2.
BIOTECHNOLOGY LETTERS
(2021)
Article
Mycology
Nohemi Garcia-Ortiz, Francisco J. Figueroa-Martinez, Ulises Carrasco-Navarro, Ernesto Favela-Torres, Octavio Loera
Article
Genetics & Heredity
Eduardo Zuniga-Leon, Ulises Carrasco-Navarro, Francisco Fierro
Article
Biochemistry & Molecular Biology
A. Ruiz-Lara, F. Fierro, U. Carrasco, J. A. Oria, A. Tomasini
PROCESS BIOCHEMISTRY
(2020)
Article
Chemistry, Applied
C. Mendarte-Alquisira, M. Gutierrez-Rojast, T. Volke-Sepulveda
REVISTA MEXICANA DE INGENIERIA QUIMICA
(2020)
Article
Biochemistry & Molecular Biology
C. Guerrero-Urrutia, T. Volke-Sepulveda, F. Figueroa-Martinez, E. Favela-Torres
Summary: In solid-state fermentation (SSF) with a high sucrose concentration, Aspergillus brasiliensis showed higher efficiency in producing extracellular inulinase and invertase compared to submerged fermentation (SmF), with a shorter lag phase observed in SSF. Enzyme activities varied under different conditions.
PROCESS BIOCHEMISTRY
(2021)
Article
Microbiology
Ulises Carrasco-Navarro, Jesus Aguirre
Summary: The study reports the impact of H2O2 on protein phosphorylation in Aspergillus nidulans during mycelial growth, revealing the importance of H2O2 in cell signaling and involvement of multiple signaling pathways.
Article
Biotechnology & Applied Microbiology
W. D. Perez-Perez, U. Carrasco-Navarro, C. Garcia-Estrada, K. Kosalkova, M. C. Gutierrez-Ruiz, J. Barrios-Gonzalez, F. Fierro
Summary: This study characterized two effectors in Penicillium chrysogenum and revealed their roles in oxidative stress response and secondary metabolism regulation. The two effectors, PcYap1 and PcRsmA, were found to bind to specific regulatory elements in key gene promoters, mediating the regulation of penicillin biosynthesis and conidiation by reactive oxygen species (ROS).
MICROBIAL CELL FACTORIES
(2022)
Review
Biochemistry & Molecular Biology
Jessica J. Hurtado-Rios, Ulises Carrasco-Navarro, Julio Cesar Almanza-Perez, Edith Ponce-Alquicira
Summary: Moonlighting proteins refer to proteins capable of performing multiple functions within the same polypeptide chain. They have potential applications in various fields, including health, pharmacology, and nutrition. Some ribosomal proteins involved in protein assembly and translation also exhibit antimicrobial activity and may have potential as alternatives to antibiotics. However, the mechanism of action of ribosomal proteins as antimicrobial peptides is not completely understood.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Fernanda J. Calderon-de la Sancha, Ulises Carrasco-Navarro, Gerardo Santander, Javier Barrios-Gonzalez, Armando Mejia
Summary: Antimicrobial activity was found in a low-molecular-weight protein secreted naturally by Streptomyces lividans TK24 when glucose or glycerol were used as carbon sources, inhibiting the growth of several bacterial species. LC-MS/MS identified a protein fraction with antimicrobial activity, and further analysis suggested that 50S ribosomal protein L19 may be the main candidate responsible for this activity.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Food Science & Technology
Monserrat Escobar-Sanchez, Ulises Carrasco-Navarro, Carmen Juarez-Castelan, Luis Lozano-Aguirre Beltran, M. Lourdes Perez-Chabela, Edith Ponce-Alquicira
Summary: Pediococcus pentosaceus 1101 was identified and evaluated for its probiotic properties under gastrointestinal conditions. The strain showed antagonistic activity, high resistance to acidity and bile salts, hydrophobic binding, and sensitivity to certain antibiotics. Genomic and proteomic analyses revealed proteins involved in acid and bile salt sensing, as well as the production of a lytic enzyme that may contribute to antimicrobial activity. These proteins may play a crucial role in the adaptation and probiotic properties of P. pentosaceus 1101 in the gastrointestinal tract.
Article
Genetics & Heredity
Elizabeth Medina-Castellanos, Daniel A. Salgado-Bautista, Juan M. Martinez-Andrade, Ruben Dario Cadena-Nava, Meritxell Riquelme
Summary: Extracellular vesicles (EVs) are nanosized structures released by living cells, playing roles in intercellular communication, virulence, and disease. This study focused on analyzing EVs in a non-pathogenic filamentous fungus, Neurospora crassa, identifying spherical membranous structures containing various proteins involved in metabolic processes, stress response, cell wall organization/remodeling, and circadian clock regulation. The findings suggest a potential role for EVs in cell wall biosynthesis and vegetative development in N. crassa.
FUNGAL GENETICS AND BIOLOGY
(2023)
Article
Microbiology
Nemi Alcantara-Martinez, Francisco Figueroa-Martinez, Fernando Rivera-Cabrera, Tania Volke-Sepulveda
Summary: This study discovered an endophytic arsenic-tolerant microalga associated with the arsenic-tolerant shrub Acacia farnesiana, expanding the known variety of plant endophytes.
FEMS MICROBIOLOGY ECOLOGY
(2022)