Journal
EMBO REPORTS
Volume 21, Issue 5, Pages -Publisher
WILEY
DOI: 10.15252/embr.201948977
Keywords
core splicing factors; flagellin; long noncoding RNA; PRP8a; SmD1b
Categories
Funding
- ANPCyT (Argentina) [PICT 2016-0289, -0007]
- CNRS (Laboratoire International Associe NOCOSYM)
- Laboratoire d'Excellence (LABEX) Saclay Plant Sciences (SPS) [ANR-10-LABX-40]
- Ministere Francais de l'Enseignement Superieur et de la Recherche
- ANR [ANR-15-CE20-0002-01 EPISYM, ANR 16-CE20-0003-04 SPLISIL]
- IPS2, France
- KAUST University, Saudi Arabia
- LabEx Saclay Plant Sciences-SPS [ANR-10-LABX-0040-SPS]
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Alternative splicing (AS) is a major source of transcriptome diversity. Long noncoding RNAs (lncRNAs) have emerged as regulators of AS through different molecular mechanisms. In Arabidopsis thaliana, the AS regulators NSRs interact with the ALTERNATIVE SPLICING COMPETITOR (ASCO) lncRNA. Here, we analyze the effect of the knock-down and overexpression of ASCO at the genome-wide level and find a large number of deregulated and differentially spliced genes related to flagellin responses and biotic stress. In agreement, ASCO-silenced plants are more sensitive to flagellin. However, only a minor subset of deregulated genes overlaps with the AS defects of the nsra/b double mutant, suggesting an alternative way of action for ASCO. Using biotin-labeled oligonucleotides for RNA-mediated ribonucleoprotein purification, we show that ASCO binds to the highly conserved spliceosome component PRP8a. ASCO overaccumulation impairs the recognition of specific flagellin-related transcripts by PRP8a. We further show that ASCO also binds to another spliceosome component, SmD1b, indicating that it interacts with multiple splicing factors. Hence, lncRNAs may integrate a dynamic network including spliceosome core proteins, to modulate transcriptome reprogramming in eukaryotes.
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