Pooled In Vitro and In Vivo CRISPR-Cas9 Screening Identifies Tumor Suppressors in Human Colon Organoids

Published 2020 View Full Article

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Title
Pooled In Vitro and In Vivo CRISPR-Cas9 Screening Identifies Tumor Suppressors in Human Colon Organoids
Authors
Keywords
patient-derived organoids, human colonic stem cells, colorectal cancer, tumor suppressor genes, pooled-barcoded CRISPR-Cas9 screening, clonal drift, lentiviral barcoding, non-homologous end joining, unique molecular identifiers, tumor microenvironment
Journal
Cell Stem Cell
Volume 26, Issue 5, Pages 782-792.e7
Publisher
Elsevier BV
Online
2020-04-28
DOI
10.1016/j.stem.2020.04.003

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