Pooled In Vitro and In Vivo CRISPR-Cas9 Screening Identifies Tumor Suppressors in Human Colon Organoids

出版年份 2020 全文链接

标题

Pooled In Vitro and In Vivo CRISPR-Cas9 Screening Identifies Tumor Suppressors in Human Colon Organoids

作者

关键词

patient-derived organoids, human colonic stem cells, colorectal cancer, tumor suppressor genes, pooled-barcoded CRISPR-Cas9 screening, clonal drift, lentiviral barcoding, non-homologous end joining, unique molecular identifiers, tumor microenvironment

出版物

Cell Stem Cell

Volume 26, Issue 5, Pages 782-792.e7

出版商

Elsevier BV

发表日期

2020-04-28

DOI

10.1016/j.stem.2020.04.003

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Protocol

Reagent

Reprint

联系作者

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